Impact of crystallinity and crystal size of nanostructured carbonated hydroxyapatite on pre‐osteoblast in vitro biocompatibility
Nanostructured carbonated hydroxyapatite (nCHA) is a promising biomaterial for bone tissue engineering due to its chemical properties, similar to those of the bone mineral phase and its enhanced in vivo bioresorption. However, the biological effects of nCHA nanoparticles on cells and tissues are not...
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| Veröffentlicht in: | Journal of biomedical materials research. Part A 2019-09, Vol.107 (9), p.1965-1976 |
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| container_end_page | 1976 |
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| container_issue | 9 |
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| container_title | Journal of biomedical materials research. Part A |
| container_volume | 107 |
| creator | dos Anjos, Suzana Mavropoulos, Elena Alves, Gutemberg G. Costa, Andrea M. de Alencar Hausen, Moema Spiegel, Carolina N. Longuinho, Mariana M. Mir, Mirta Granjeiro, José M. Rossi, Alexandre M. |
| description | Nanostructured carbonated hydroxyapatite (nCHA) is a promising biomaterial for bone tissue engineering due to its chemical properties, similar to those of the bone mineral phase and its enhanced in vivo bioresorption. However, the biological effects of nCHA nanoparticles on cells and tissues are not sufficiently known. This study assessed the impact of exposing pre‐osteoblasts to suspensions with high doses of nCHA nanoparticles with high or low crystallinity. MC3T3‐E1 pre‐osteoblasts were cultured for 1 or 7 days in a culture medium previously exposed to CHA nanoparticles for 1 day. Control groups were produced by centrifugation for removal of bigger nCHA aggregates before exposure. Interaction of nanoparticles with the culture medium drastically changed medium composition, promoting Ca, P, and protein adsorption. Transmission Electron microscopy revealed that exposed cells were able to internalize both materials, which seemed concentrated inside endosomes. No cytotoxicity was observed for both materials, regardless of centrifugation, and the exposure did not induce alterations in the release of pro‐and anti‐inflammatory cytokines. Morphological analysis revealed strong interactions of nCHA aggregates with cell surfaces, however without marked alterations in morphological features and cytoskeleton ultrastructure. The overall in vitro biocompatibility of nCHA materials, regardless of physicochemical characteristics such as crystallinity, encourages further studies on their clinical applications. |
| doi_str_mv | 10.1002/jbm.a.36709 |
| format | Article |
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However, the biological effects of nCHA nanoparticles on cells and tissues are not sufficiently known. This study assessed the impact of exposing pre‐osteoblasts to suspensions with high doses of nCHA nanoparticles with high or low crystallinity. MC3T3‐E1 pre‐osteoblasts were cultured for 1 or 7 days in a culture medium previously exposed to CHA nanoparticles for 1 day. Control groups were produced by centrifugation for removal of bigger nCHA aggregates before exposure. Interaction of nanoparticles with the culture medium drastically changed medium composition, promoting Ca, P, and protein adsorption. Transmission Electron microscopy revealed that exposed cells were able to internalize both materials, which seemed concentrated inside endosomes. No cytotoxicity was observed for both materials, regardless of centrifugation, and the exposure did not induce alterations in the release of pro‐and anti‐inflammatory cytokines. Morphological analysis revealed strong interactions of nCHA aggregates with cell surfaces, however without marked alterations in morphological features and cytoskeleton ultrastructure. The overall in vitro biocompatibility of nCHA materials, regardless of physicochemical characteristics such as crystallinity, encourages further studies on their clinical applications.</description><identifier>ISSN: 1549-3296</identifier><identifier>EISSN: 1552-4965</identifier><identifier>DOI: 10.1002/jbm.a.36709</identifier><identifier>PMID: 31035306</identifier><language>eng</language><publisher>Hoboken, USA: John Wiley & Sons, Inc</publisher><subject>Aggregates ; Biocompatibility ; Biological effects ; Biomaterials ; Biomedical materials ; carbonated hydroxiapatite ; Carbonation ; Cell culture ; Centrifugation ; Chemical properties ; Crystal structure ; Crystallinity ; Cytokines ; Cytoskeleton ; Cytotoxicity ; Endosomes ; Exposure ; Hydroxyapatite ; Inflammation ; Morphology ; Nanoparticles ; Nanostructure ; Organic chemistry ; osteoblast ; Osteoblasts ; Protein adsorption ; Surgical implants ; Therapeutic applications ; Tissue engineering ; Toxicity ; Transmission electron microscopy ; Ultrastructure</subject><ispartof>Journal of biomedical materials research. 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Part A</title><addtitle>J Biomed Mater Res A</addtitle><description>Nanostructured carbonated hydroxyapatite (nCHA) is a promising biomaterial for bone tissue engineering due to its chemical properties, similar to those of the bone mineral phase and its enhanced in vivo bioresorption. However, the biological effects of nCHA nanoparticles on cells and tissues are not sufficiently known. This study assessed the impact of exposing pre‐osteoblasts to suspensions with high doses of nCHA nanoparticles with high or low crystallinity. MC3T3‐E1 pre‐osteoblasts were cultured for 1 or 7 days in a culture medium previously exposed to CHA nanoparticles for 1 day. Control groups were produced by centrifugation for removal of bigger nCHA aggregates before exposure. Interaction of nanoparticles with the culture medium drastically changed medium composition, promoting Ca, P, and protein adsorption. Transmission Electron microscopy revealed that exposed cells were able to internalize both materials, which seemed concentrated inside endosomes. No cytotoxicity was observed for both materials, regardless of centrifugation, and the exposure did not induce alterations in the release of pro‐and anti‐inflammatory cytokines. Morphological analysis revealed strong interactions of nCHA aggregates with cell surfaces, however without marked alterations in morphological features and cytoskeleton ultrastructure. The overall in vitro biocompatibility of nCHA materials, regardless of physicochemical characteristics such as crystallinity, encourages further studies on their clinical applications.</description><subject>Aggregates</subject><subject>Biocompatibility</subject><subject>Biological effects</subject><subject>Biomaterials</subject><subject>Biomedical materials</subject><subject>carbonated hydroxiapatite</subject><subject>Carbonation</subject><subject>Cell culture</subject><subject>Centrifugation</subject><subject>Chemical properties</subject><subject>Crystal structure</subject><subject>Crystallinity</subject><subject>Cytokines</subject><subject>Cytoskeleton</subject><subject>Cytotoxicity</subject><subject>Endosomes</subject><subject>Exposure</subject><subject>Hydroxyapatite</subject><subject>Inflammation</subject><subject>Morphology</subject><subject>Nanoparticles</subject><subject>Nanostructure</subject><subject>Organic chemistry</subject><subject>osteoblast</subject><subject>Osteoblasts</subject><subject>Protein adsorption</subject><subject>Surgical implants</subject><subject>Therapeutic applications</subject><subject>Tissue engineering</subject><subject>Toxicity</subject><subject>Transmission electron microscopy</subject><subject>Ultrastructure</subject><issn>1549-3296</issn><issn>1552-4965</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNp90b1u1TAYBmALgWhpO3VHkbogoRz8EzvHY6n4KSpiaWfrs-OoPkrsYDtAmBBXwDVyJTiclqEDkz_Zj17behE6JXhDMKavdnrcwIaJFstH6JBwTutGCv54nRtZMyrFAXqW0q5ggTl9ig4YwYwzLA7Rz8txApOr0FcmLinDMDjv8lKB7-53quS-21V48CHlOJs8R1uOIergIZfxduli-LbABNnlYn01Rfv7x6_CbdADpFw5X31xOYZKu2DCuErthnLVMXrSw5Dsyd16hG7evrm-eF9ffXp3eXF-VRsmsKxp0zXQ09YA5b00Gjrd8EZoYThrCZOcsh4M2TLLQVrRlc8zth71tmuo3LIj9GKfO8XwebYpq9ElY4cBvA1zUpSStpGMbGWhZw_oLszRl9cVxQWXQra8qJd7ZWJIKdpeTdGNEBdFsFqrUaUaBepvNUU_v8uc9Wi7f_a-iwLoHnx1g13-l6U-vP54vk_9A99ZnWs</recordid><startdate>201909</startdate><enddate>201909</enddate><creator>dos Anjos, Suzana</creator><creator>Mavropoulos, Elena</creator><creator>Alves, Gutemberg G.</creator><creator>Costa, Andrea M.</creator><creator>de Alencar Hausen, Moema</creator><creator>Spiegel, Carolina N.</creator><creator>Longuinho, Mariana M.</creator><creator>Mir, Mirta</creator><creator>Granjeiro, José M.</creator><creator>Rossi, Alexandre M.</creator><general>John Wiley & Sons, Inc</general><general>Wiley Subscription Services, Inc</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>JG9</scope><scope>JQ2</scope><scope>K9.</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>201909</creationdate><title>Impact of crystallinity and crystal size of nanostructured carbonated hydroxyapatite on pre‐osteoblast in vitro biocompatibility</title><author>dos Anjos, Suzana ; Mavropoulos, Elena ; Alves, Gutemberg G. ; Costa, Andrea M. ; de Alencar Hausen, Moema ; Spiegel, Carolina N. ; Longuinho, Mariana M. ; Mir, Mirta ; Granjeiro, José M. ; Rossi, Alexandre M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3609-24d4af27ca25f9cbadb4546b6c537139523fac183e5a9e6d154335371fed42983</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Aggregates</topic><topic>Biocompatibility</topic><topic>Biological effects</topic><topic>Biomaterials</topic><topic>Biomedical materials</topic><topic>carbonated hydroxiapatite</topic><topic>Carbonation</topic><topic>Cell culture</topic><topic>Centrifugation</topic><topic>Chemical properties</topic><topic>Crystal structure</topic><topic>Crystallinity</topic><topic>Cytokines</topic><topic>Cytoskeleton</topic><topic>Cytotoxicity</topic><topic>Endosomes</topic><topic>Exposure</topic><topic>Hydroxyapatite</topic><topic>Inflammation</topic><topic>Morphology</topic><topic>Nanoparticles</topic><topic>Nanostructure</topic><topic>Organic chemistry</topic><topic>osteoblast</topic><topic>Osteoblasts</topic><topic>Protein adsorption</topic><topic>Surgical implants</topic><topic>Therapeutic applications</topic><topic>Tissue engineering</topic><topic>Toxicity</topic><topic>Transmission electron microscopy</topic><topic>Ultrastructure</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>dos Anjos, Suzana</creatorcontrib><creatorcontrib>Mavropoulos, Elena</creatorcontrib><creatorcontrib>Alves, Gutemberg G.</creatorcontrib><creatorcontrib>Costa, Andrea M.</creatorcontrib><creatorcontrib>de Alencar Hausen, Moema</creatorcontrib><creatorcontrib>Spiegel, Carolina N.</creatorcontrib><creatorcontrib>Longuinho, Mariana M.</creatorcontrib><creatorcontrib>Mir, Mirta</creatorcontrib><creatorcontrib>Granjeiro, José M.</creatorcontrib><creatorcontrib>Rossi, Alexandre M.</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of biomedical materials research. Part A</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>dos Anjos, Suzana</au><au>Mavropoulos, Elena</au><au>Alves, Gutemberg G.</au><au>Costa, Andrea M.</au><au>de Alencar Hausen, Moema</au><au>Spiegel, Carolina N.</au><au>Longuinho, Mariana M.</au><au>Mir, Mirta</au><au>Granjeiro, José M.</au><au>Rossi, Alexandre M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Impact of crystallinity and crystal size of nanostructured carbonated hydroxyapatite on pre‐osteoblast in vitro biocompatibility</atitle><jtitle>Journal of biomedical materials research. Part A</jtitle><addtitle>J Biomed Mater Res A</addtitle><date>2019-09</date><risdate>2019</risdate><volume>107</volume><issue>9</issue><spage>1965</spage><epage>1976</epage><pages>1965-1976</pages><issn>1549-3296</issn><eissn>1552-4965</eissn><abstract>Nanostructured carbonated hydroxyapatite (nCHA) is a promising biomaterial for bone tissue engineering due to its chemical properties, similar to those of the bone mineral phase and its enhanced in vivo bioresorption. However, the biological effects of nCHA nanoparticles on cells and tissues are not sufficiently known. This study assessed the impact of exposing pre‐osteoblasts to suspensions with high doses of nCHA nanoparticles with high or low crystallinity. MC3T3‐E1 pre‐osteoblasts were cultured for 1 or 7 days in a culture medium previously exposed to CHA nanoparticles for 1 day. Control groups were produced by centrifugation for removal of bigger nCHA aggregates before exposure. Interaction of nanoparticles with the culture medium drastically changed medium composition, promoting Ca, P, and protein adsorption. Transmission Electron microscopy revealed that exposed cells were able to internalize both materials, which seemed concentrated inside endosomes. No cytotoxicity was observed for both materials, regardless of centrifugation, and the exposure did not induce alterations in the release of pro‐and anti‐inflammatory cytokines. Morphological analysis revealed strong interactions of nCHA aggregates with cell surfaces, however without marked alterations in morphological features and cytoskeleton ultrastructure. The overall in vitro biocompatibility of nCHA materials, regardless of physicochemical characteristics such as crystallinity, encourages further studies on their clinical applications.</abstract><cop>Hoboken, USA</cop><pub>John Wiley & Sons, Inc</pub><pmid>31035306</pmid><doi>10.1002/jbm.a.36709</doi><tpages>12</tpages></addata></record> |
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| subjects | Aggregates Biocompatibility Biological effects Biomaterials Biomedical materials carbonated hydroxiapatite Carbonation Cell culture Centrifugation Chemical properties Crystal structure Crystallinity Cytokines Cytoskeleton Cytotoxicity Endosomes Exposure Hydroxyapatite Inflammation Morphology Nanoparticles Nanostructure Organic chemistry osteoblast Osteoblasts Protein adsorption Surgical implants Therapeutic applications Tissue engineering Toxicity Transmission electron microscopy Ultrastructure |
| title | Impact of crystallinity and crystal size of nanostructured carbonated hydroxyapatite on pre‐osteoblast in vitro biocompatibility |
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