Identification of reference genes for microRNAs of extracellular vesicles isolated from plasma samples of healthy dogs by ultracentrifugation, precipitation, and membrane affinity chromatography methods

To compare ultracentrifugation, precipitation, and membrane affinity chromatography methods for isolation of extracellular vesicles (EVs) from canine plasma samples and to identify suitable reference genes for incorporation into a quantitative reverse transcription PCR assay of microRNA expression i...

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Veröffentlicht in:	American journal of veterinary research 2019-05, Vol.80 (5), p.449-454
Hauptverfasser:	Narita, Momoko, Nishida, Hidetaka, Asahina, Ryota, Nakata, Kohei, Yano, Hirohito, Ueda, Tomoyuki, Inden, Masatoshi, Akiyoshi, Hideo, Maeda, Sadatoshi, Kamishina, Hiroaki
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Chromatography, Affinity - veterinary Dogs - genetics Extracellular Vesicles - genetics Female Male MicroRNAs - genetics Nanoparticles Real-Time Polymerase Chain Reaction - veterinary Ultracentrifugation - veterinary
Online-Zugang:	Volltext
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