Instantaneous isotropic volumetric imaging of fast biological processes

To capture highly dynamic biological processes at cellular resolution is a recurring challenge in biology. Here we show that combining selective-volume illumination with simultaneous acquisition of orthogonal light fields yields three-dimensional images with high, isotropic spatial resolution and a...

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Veröffentlicht in:	Nature methods 2019-06, Vol.16 (6), p.497-500
Hauptverfasser:	Wagner, Nils, Norlin, Nils, Gierten, Jakob, de Medeiros, Gustavo, Balázs, Bálint, Wittbrodt, Joachim, Hufnagel, Lars, Prevedel, Robert
Format:	Artikel
Sprache:	eng
Schlagworte:	631/136 631/1647/245 631/1647/328 631/1647/328/2237 631/57/2266 Algorithms Analysis Animals Animals, Genetically Modified Artefacts Bioinformatics Biological activity Biological Microscopy Biological systems Biological Techniques Biomedical and Life Sciences Biomedical Engineering/Biotechnology Biophysics Blood flow Blood Flow Velocity Brief Communication Cameras Heart Heart - diagnostic imaging Heart - physiology Humans Image acquisition Image Processing, Computer-Assisted - methods Image reconstruction Imaging, Three-Dimensional - methods Laboratories Life Sciences Light Methods Microscope and microscopy Microscopy Microscopy - methods Molecular biology Neurosciences Objectives Oryzias Performance evaluation Proteomics Single-Cell Analysis - methods Spatial discrimination Spatial resolution Three dimensional imaging
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