Involvement of the epithelial sodium channel in initiation of mechanically evoked swallows in anaesthetized rats
Key points Afferents carried by the superior laryngeal nerve play a primary role in the initiation of laryngeal mechanically evoked swallows in anaesthetized rats. Amiloride and its analogues inhibit swallowing evoked by mechanical stimulation, but not swallowing evoked by chemical and electrical st...
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| Veröffentlicht in: | The Journal of physiology 2019-06, Vol.597 (11), p.2949-2963 |
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| creator | Tsujimura, Takanori Ueha, Rumi Yoshihara, Midori Takei, Eri Nagoya, Kouta Shiraishi, Naru Magara, Jin Inoue, Makoto |
| description | Key points
Afferents carried by the superior laryngeal nerve play a primary role in the initiation of laryngeal mechanically evoked swallows in anaesthetized rats.
Amiloride and its analogues inhibit swallowing evoked by mechanical stimulation, but not swallowing evoked by chemical and electrical stimulation.
The epithelial sodium channel is probably involved in the initiation of laryngeal mechanically evoked swallows.
The swallowing reflex plays a critical role in airway protection. Because impaired laryngeal mechanosensation is associated with food bolus aspiration, it is important to know how the laryngeal sensory system regulates swallowing initiation. This study was performed to clarify the neuronal mechanism of mechanically evoked swallows. Urethane‐anaesthetized Sprague–Dawley male rats were used. A swallow was identified by activation of the suprahyoid and thyrohyoid muscles on electromyography. The swallowing threshold was measured by von Frey filament and electrical stimulation of the larynx. The number of swallows induced by upper airway distension and capsaicin application (0.03 nmol, 3 μl) to the vocal folds was counted. The effects of topical application (0.3–30 nmol, 3 μl) of the epithelial sodium channel (ENaC) blocker amiloride and its analogues (benzamil and dimethylamiloride), acid‐sensing ion channel (ASIC) inhibitors (mambalgine‐1 and diminazene) and gadolinium to the laryngeal mucosa on swallowing initiation were evaluated. A nerve transection study indicated that afferents carried by the superior laryngeal nerve play a primary role in the initiation of laryngeal mechanically evoked swallows. The mechanical threshold of swallowing was increased in a dose‐dependent manner by amiloride and its analogues and gadolinium, but not by ASIC inhibitors. The number of swallows by upper airway distension was significantly decreased by benzamil application. However, the initiation of swallows evoked by capsaicin and electrical stimulation was not affected by benzamil application. We speculate that the ENaC is involved in the initiation of laryngeal mechanically evoked swallows.
Key points
Afferents carried by the superior laryngeal nerve play a primary role in the initiation of laryngeal mechanically evoked swallows in anaesthetized rats.
Amiloride and its analogues inhibit swallowing evoked by mechanical stimulation, but not swallowing evoked by chemical and electrical stimulation.
The epithelial sodium channel is probably involved in the initia |
| doi_str_mv | 10.1113/JP277895 |
| format | Article |
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Afferents carried by the superior laryngeal nerve play a primary role in the initiation of laryngeal mechanically evoked swallows in anaesthetized rats.
Amiloride and its analogues inhibit swallowing evoked by mechanical stimulation, but not swallowing evoked by chemical and electrical stimulation.
The epithelial sodium channel is probably involved in the initiation of laryngeal mechanically evoked swallows.
The swallowing reflex plays a critical role in airway protection. Because impaired laryngeal mechanosensation is associated with food bolus aspiration, it is important to know how the laryngeal sensory system regulates swallowing initiation. This study was performed to clarify the neuronal mechanism of mechanically evoked swallows. Urethane‐anaesthetized Sprague–Dawley male rats were used. A swallow was identified by activation of the suprahyoid and thyrohyoid muscles on electromyography. The swallowing threshold was measured by von Frey filament and electrical stimulation of the larynx. The number of swallows induced by upper airway distension and capsaicin application (0.03 nmol, 3 μl) to the vocal folds was counted. The effects of topical application (0.3–30 nmol, 3 μl) of the epithelial sodium channel (ENaC) blocker amiloride and its analogues (benzamil and dimethylamiloride), acid‐sensing ion channel (ASIC) inhibitors (mambalgine‐1 and diminazene) and gadolinium to the laryngeal mucosa on swallowing initiation were evaluated. A nerve transection study indicated that afferents carried by the superior laryngeal nerve play a primary role in the initiation of laryngeal mechanically evoked swallows. The mechanical threshold of swallowing was increased in a dose‐dependent manner by amiloride and its analogues and gadolinium, but not by ASIC inhibitors. The number of swallows by upper airway distension was significantly decreased by benzamil application. However, the initiation of swallows evoked by capsaicin and electrical stimulation was not affected by benzamil application. We speculate that the ENaC is involved in the initiation of laryngeal mechanically evoked swallows.
Key points
Afferents carried by the superior laryngeal nerve play a primary role in the initiation of laryngeal mechanically evoked swallows in anaesthetized rats.
Amiloride and its analogues inhibit swallowing evoked by mechanical stimulation, but not swallowing evoked by chemical and electrical stimulation.
The epithelial sodium channel is probably involved in the initiation of laryngeal mechanically evoked swallows.</description><identifier>ISSN: 0022-3751</identifier><identifier>EISSN: 1469-7793</identifier><identifier>DOI: 10.1113/JP277895</identifier><identifier>PMID: 31032906</identifier><language>eng</language><publisher>England: Wiley Subscription Services, Inc</publisher><subject>Acidity ; Amiloride ; Capsaicin ; Distension ; Electrical stimuli ; Electromyography ; epithelial sodium channel ; Ethyl carbamate ; Gadolinium ; Larynx ; mechanical stimulation ; Mechanotransduction ; Mucosa ; Muscles ; Respiratory tract ; Sodium ; Sodium channels ; Superior laryngeal nerve ; Swallowing ; Topical application</subject><ispartof>The Journal of physiology, 2019-06, Vol.597 (11), p.2949-2963</ispartof><rights>2019 The Authors. The Journal of Physiology © 2019 The Physiological Society</rights><rights>2019 The Authors. The Journal of Physiology © 2019 The Physiological Society.</rights><rights>Journal compilation © 2019 The Physiological Society</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3844-427316d30ac0478401aaed3f08ae6120bf6c36320b26bfe43d8773b69c967a3c3</citedby><cites>FETCH-LOGICAL-c3844-427316d30ac0478401aaed3f08ae6120bf6c36320b26bfe43d8773b69c967a3c3</cites><orcidid>0000-0002-0608-7237 ; 0000-0003-4552-8813 ; 0000-0002-6503-2973</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1113%2FJP277895$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1113%2FJP277895$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,1427,27901,27902,45550,45551,46384,46808</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31032906$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tsujimura, Takanori</creatorcontrib><creatorcontrib>Ueha, Rumi</creatorcontrib><creatorcontrib>Yoshihara, Midori</creatorcontrib><creatorcontrib>Takei, Eri</creatorcontrib><creatorcontrib>Nagoya, Kouta</creatorcontrib><creatorcontrib>Shiraishi, Naru</creatorcontrib><creatorcontrib>Magara, Jin</creatorcontrib><creatorcontrib>Inoue, Makoto</creatorcontrib><title>Involvement of the epithelial sodium channel in initiation of mechanically evoked swallows in anaesthetized rats</title><title>The Journal of physiology</title><addtitle>J Physiol</addtitle><description>Key points
Afferents carried by the superior laryngeal nerve play a primary role in the initiation of laryngeal mechanically evoked swallows in anaesthetized rats.
Amiloride and its analogues inhibit swallowing evoked by mechanical stimulation, but not swallowing evoked by chemical and electrical stimulation.
The epithelial sodium channel is probably involved in the initiation of laryngeal mechanically evoked swallows.
The swallowing reflex plays a critical role in airway protection. Because impaired laryngeal mechanosensation is associated with food bolus aspiration, it is important to know how the laryngeal sensory system regulates swallowing initiation. This study was performed to clarify the neuronal mechanism of mechanically evoked swallows. Urethane‐anaesthetized Sprague–Dawley male rats were used. A swallow was identified by activation of the suprahyoid and thyrohyoid muscles on electromyography. The swallowing threshold was measured by von Frey filament and electrical stimulation of the larynx. The number of swallows induced by upper airway distension and capsaicin application (0.03 nmol, 3 μl) to the vocal folds was counted. The effects of topical application (0.3–30 nmol, 3 μl) of the epithelial sodium channel (ENaC) blocker amiloride and its analogues (benzamil and dimethylamiloride), acid‐sensing ion channel (ASIC) inhibitors (mambalgine‐1 and diminazene) and gadolinium to the laryngeal mucosa on swallowing initiation were evaluated. A nerve transection study indicated that afferents carried by the superior laryngeal nerve play a primary role in the initiation of laryngeal mechanically evoked swallows. The mechanical threshold of swallowing was increased in a dose‐dependent manner by amiloride and its analogues and gadolinium, but not by ASIC inhibitors. The number of swallows by upper airway distension was significantly decreased by benzamil application. However, the initiation of swallows evoked by capsaicin and electrical stimulation was not affected by benzamil application. We speculate that the ENaC is involved in the initiation of laryngeal mechanically evoked swallows.
Key points
Afferents carried by the superior laryngeal nerve play a primary role in the initiation of laryngeal mechanically evoked swallows in anaesthetized rats.
Amiloride and its analogues inhibit swallowing evoked by mechanical stimulation, but not swallowing evoked by chemical and electrical stimulation.
The epithelial sodium channel is probably involved in the initiation of laryngeal mechanically evoked swallows.</description><subject>Acidity</subject><subject>Amiloride</subject><subject>Capsaicin</subject><subject>Distension</subject><subject>Electrical stimuli</subject><subject>Electromyography</subject><subject>epithelial sodium channel</subject><subject>Ethyl carbamate</subject><subject>Gadolinium</subject><subject>Larynx</subject><subject>mechanical stimulation</subject><subject>Mechanotransduction</subject><subject>Mucosa</subject><subject>Muscles</subject><subject>Respiratory tract</subject><subject>Sodium</subject><subject>Sodium channels</subject><subject>Superior laryngeal nerve</subject><subject>Swallowing</subject><subject>Topical application</subject><issn>0022-3751</issn><issn>1469-7793</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNp1kdtKxDAQhoMouh7AJ5CCN95Uk0w2aS9FPCK4F-t1yaZTjKbJ2rS7rE9vlvUAgjDwT5iPjyFDyDGj54wxuHiYcKWKcrxFRkzIMleqhG0yopTzHNSY7ZH9GF8pZUDLcpfsAaPASypHZH7vF8EtsEXfZ6HJ-hfMcG5TOKtdFkNthzYzL9p7dJn1qWxvdW-DX-MtrkfWaOdWGS7CG9ZZXKZXWMY1rb3GmGS9_UiTTvfxkOw02kU8-soD8nxzPb26yx-fbu-vLh9zA4UQueAKmKyBakOFKgRlWmMNDS00SsbprJEGJKSGy1mDAupCKZjJ0pRSaTBwQM423nkX3oe0RNXaaNA57TEMseKcSaUESJXQ0z_oaxg6n7ZLFHAuxyl-haYLMXbYVPPOtrpbVYxW6ytU31dI6MmXcJi1WP-A39-egPMNsLQOV_-KqunDhMEYBHwCipOP4g</recordid><startdate>20190601</startdate><enddate>20190601</enddate><creator>Tsujimura, Takanori</creator><creator>Ueha, Rumi</creator><creator>Yoshihara, Midori</creator><creator>Takei, Eri</creator><creator>Nagoya, Kouta</creator><creator>Shiraishi, Naru</creator><creator>Magara, Jin</creator><creator>Inoue, Makoto</creator><general>Wiley Subscription Services, Inc</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>7TS</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-0608-7237</orcidid><orcidid>https://orcid.org/0000-0003-4552-8813</orcidid><orcidid>https://orcid.org/0000-0002-6503-2973</orcidid></search><sort><creationdate>20190601</creationdate><title>Involvement of the epithelial sodium channel in initiation of mechanically evoked swallows in anaesthetized rats</title><author>Tsujimura, Takanori ; Ueha, Rumi ; Yoshihara, Midori ; Takei, Eri ; Nagoya, Kouta ; Shiraishi, Naru ; Magara, Jin ; Inoue, Makoto</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3844-427316d30ac0478401aaed3f08ae6120bf6c36320b26bfe43d8773b69c967a3c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Acidity</topic><topic>Amiloride</topic><topic>Capsaicin</topic><topic>Distension</topic><topic>Electrical stimuli</topic><topic>Electromyography</topic><topic>epithelial sodium channel</topic><topic>Ethyl carbamate</topic><topic>Gadolinium</topic><topic>Larynx</topic><topic>mechanical stimulation</topic><topic>Mechanotransduction</topic><topic>Mucosa</topic><topic>Muscles</topic><topic>Respiratory tract</topic><topic>Sodium</topic><topic>Sodium channels</topic><topic>Superior laryngeal nerve</topic><topic>Swallowing</topic><topic>Topical application</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tsujimura, Takanori</creatorcontrib><creatorcontrib>Ueha, Rumi</creatorcontrib><creatorcontrib>Yoshihara, Midori</creatorcontrib><creatorcontrib>Takei, Eri</creatorcontrib><creatorcontrib>Nagoya, Kouta</creatorcontrib><creatorcontrib>Shiraishi, Naru</creatorcontrib><creatorcontrib>Magara, Jin</creatorcontrib><creatorcontrib>Inoue, Makoto</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Physical Education Index</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tsujimura, Takanori</au><au>Ueha, Rumi</au><au>Yoshihara, Midori</au><au>Takei, Eri</au><au>Nagoya, Kouta</au><au>Shiraishi, Naru</au><au>Magara, Jin</au><au>Inoue, Makoto</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Involvement of the epithelial sodium channel in initiation of mechanically evoked swallows in anaesthetized rats</atitle><jtitle>The Journal of physiology</jtitle><addtitle>J Physiol</addtitle><date>2019-06-01</date><risdate>2019</risdate><volume>597</volume><issue>11</issue><spage>2949</spage><epage>2963</epage><pages>2949-2963</pages><issn>0022-3751</issn><eissn>1469-7793</eissn><abstract>Key points
Afferents carried by the superior laryngeal nerve play a primary role in the initiation of laryngeal mechanically evoked swallows in anaesthetized rats.
Amiloride and its analogues inhibit swallowing evoked by mechanical stimulation, but not swallowing evoked by chemical and electrical stimulation.
The epithelial sodium channel is probably involved in the initiation of laryngeal mechanically evoked swallows.
The swallowing reflex plays a critical role in airway protection. Because impaired laryngeal mechanosensation is associated with food bolus aspiration, it is important to know how the laryngeal sensory system regulates swallowing initiation. This study was performed to clarify the neuronal mechanism of mechanically evoked swallows. Urethane‐anaesthetized Sprague–Dawley male rats were used. A swallow was identified by activation of the suprahyoid and thyrohyoid muscles on electromyography. The swallowing threshold was measured by von Frey filament and electrical stimulation of the larynx. The number of swallows induced by upper airway distension and capsaicin application (0.03 nmol, 3 μl) to the vocal folds was counted. The effects of topical application (0.3–30 nmol, 3 μl) of the epithelial sodium channel (ENaC) blocker amiloride and its analogues (benzamil and dimethylamiloride), acid‐sensing ion channel (ASIC) inhibitors (mambalgine‐1 and diminazene) and gadolinium to the laryngeal mucosa on swallowing initiation were evaluated. A nerve transection study indicated that afferents carried by the superior laryngeal nerve play a primary role in the initiation of laryngeal mechanically evoked swallows. The mechanical threshold of swallowing was increased in a dose‐dependent manner by amiloride and its analogues and gadolinium, but not by ASIC inhibitors. The number of swallows by upper airway distension was significantly decreased by benzamil application. However, the initiation of swallows evoked by capsaicin and electrical stimulation was not affected by benzamil application. We speculate that the ENaC is involved in the initiation of laryngeal mechanically evoked swallows.
Key points
Afferents carried by the superior laryngeal nerve play a primary role in the initiation of laryngeal mechanically evoked swallows in anaesthetized rats.
Amiloride and its analogues inhibit swallowing evoked by mechanical stimulation, but not swallowing evoked by chemical and electrical stimulation.
The epithelial sodium channel is probably involved in the initiation of laryngeal mechanically evoked swallows.</abstract><cop>England</cop><pub>Wiley Subscription Services, Inc</pub><pmid>31032906</pmid><doi>10.1113/JP277895</doi><tpages>15</tpages><orcidid>https://orcid.org/0000-0002-0608-7237</orcidid><orcidid>https://orcid.org/0000-0003-4552-8813</orcidid><orcidid>https://orcid.org/0000-0002-6503-2973</orcidid><oa>free_for_read</oa></addata></record> |
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| source | Wiley Online Library Journals Frontfile Complete; Wiley Online Library Free Content; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central |
| subjects | Acidity Amiloride Capsaicin Distension Electrical stimuli Electromyography epithelial sodium channel Ethyl carbamate Gadolinium Larynx mechanical stimulation Mechanotransduction Mucosa Muscles Respiratory tract Sodium Sodium channels Superior laryngeal nerve Swallowing Topical application |
| title | Involvement of the epithelial sodium channel in initiation of mechanically evoked swallows in anaesthetized rats |
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