Identification and functional characterization of polymorphisms in promoter sequences of porcine NOD1 and NOD2 genes
NOD-like receptors (NLRs) play a key role in the innate immune system, acting as a second line of surveillance against pathogens. NLRs detect particular bacteria that have gained access to the cytoplasm, evading recognition by other pattern recognition receptors, such as Toll-like receptors. It has...
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| Veröffentlicht in: | Research in veterinary science 2019-06, Vol.124, p.310-316 |
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| creator | Domínguez, Miguel A. Landi, Vincenzo Morera, Luis Martínez, Amparo Jiménez-Marín, Ángeles Garrido, Juan J. |
| description | NOD-like receptors (NLRs) play a key role in the innate immune system, acting as a second line of surveillance against pathogens. NLRs detect particular bacteria that have gained access to the cytoplasm, evading recognition by other pattern recognition receptors, such as Toll-like receptors. It has been demonstrated that coding sequence-single nucleotide polymorphisms may alter the ligand recognition ability of NLRs, affecting their pathogen-sensing function. However, there have been no data relating to the identification and functional analysis of SNPs in porcine NLR promoters. We examined the promoter sequences of the porcine NOD1 and NOD2 genes with the aim to identify and to evaluate the effect of genetic variations on promoter activity. Six SNPs in NOD1 and three SNPs in NOD2 were identified. Luciferase reporter gene assays showed significant differences in promoter activity between allele variants of NOD1 -920G>A (NC_010460.4:g.42431413G>A) and NOD2 -1670G>A (NC_010448.4:g.34169122T>C) SNPs. The results suggest that promoter polymorphisms could modify the expression levels of porcine NOD1 and NOD2 genes.
•NOD genes play a critical role in initiating the development of immune response•Analysis of SNP will expand the knowledge of genetic resistance to pathogens•Promoter polymorphisms modify the expression levels of porcine NOD genes |
| doi_str_mv | 10.1016/j.rvsc.2019.04.009 |
| format | Article |
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•NOD genes play a critical role in initiating the development of immune response•Analysis of SNP will expand the knowledge of genetic resistance to pathogens•Promoter polymorphisms modify the expression levels of porcine NOD genes</description><identifier>ISSN: 0034-5288</identifier><identifier>EISSN: 1532-2661</identifier><identifier>DOI: 10.1016/j.rvsc.2019.04.009</identifier><identifier>PMID: 31030118</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Bacterial infections ; Binding sites ; Chromosomes ; Cytoplasm ; Deoxyribonucleic acid ; DNA ; Functional analysis ; Gene expression ; Gene sequencing ; Genes ; Genetic diversity ; Genetic testing ; Haplotypes ; Hogs ; Immune system ; Innate immunity ; Mutation ; NOD ; Nod1 protein ; NOD2 protein ; Pathogen receptor ; Pathogens ; Pattern recognition ; Pattern recognition receptors ; Polymorphism ; Porcine ; Promoter ; Proteins ; Receptors ; Reporter gene ; Single-nucleotide polymorphism ; Toll-like receptors ; Transcription factors ; Veterinary medicine</subject><ispartof>Research in veterinary science, 2019-06, Vol.124, p.310-316</ispartof><rights>2019 Elsevier Ltd</rights><rights>Copyright © 2019 Elsevier Ltd. All rights reserved.</rights><rights>2019. Elsevier Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c384t-7672948938364e6dea039dac290773bbb0a607e797e97a7e6b5a05c8b91a635e3</citedby><cites>FETCH-LOGICAL-c384t-7672948938364e6dea039dac290773bbb0a607e797e97a7e6b5a05c8b91a635e3</cites><orcidid>0000-0003-1385-8439</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.rvsc.2019.04.009$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,781,785,3551,27929,27930,46000</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31030118$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Domínguez, Miguel A.</creatorcontrib><creatorcontrib>Landi, Vincenzo</creatorcontrib><creatorcontrib>Morera, Luis</creatorcontrib><creatorcontrib>Martínez, Amparo</creatorcontrib><creatorcontrib>Jiménez-Marín, Ángeles</creatorcontrib><creatorcontrib>Garrido, Juan J.</creatorcontrib><title>Identification and functional characterization of polymorphisms in promoter sequences of porcine NOD1 and NOD2 genes</title><title>Research in veterinary science</title><addtitle>Res Vet Sci</addtitle><description>NOD-like receptors (NLRs) play a key role in the innate immune system, acting as a second line of surveillance against pathogens. NLRs detect particular bacteria that have gained access to the cytoplasm, evading recognition by other pattern recognition receptors, such as Toll-like receptors. It has been demonstrated that coding sequence-single nucleotide polymorphisms may alter the ligand recognition ability of NLRs, affecting their pathogen-sensing function. However, there have been no data relating to the identification and functional analysis of SNPs in porcine NLR promoters. We examined the promoter sequences of the porcine NOD1 and NOD2 genes with the aim to identify and to evaluate the effect of genetic variations on promoter activity. Six SNPs in NOD1 and three SNPs in NOD2 were identified. Luciferase reporter gene assays showed significant differences in promoter activity between allele variants of NOD1 -920G>A (NC_010460.4:g.42431413G>A) and NOD2 -1670G>A (NC_010448.4:g.34169122T>C) SNPs. The results suggest that promoter polymorphisms could modify the expression levels of porcine NOD1 and NOD2 genes.
•NOD genes play a critical role in initiating the development of immune response•Analysis of SNP will expand the knowledge of genetic resistance to pathogens•Promoter polymorphisms modify the expression levels of porcine NOD genes</description><subject>Bacterial infections</subject><subject>Binding sites</subject><subject>Chromosomes</subject><subject>Cytoplasm</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>Functional analysis</subject><subject>Gene expression</subject><subject>Gene sequencing</subject><subject>Genes</subject><subject>Genetic diversity</subject><subject>Genetic testing</subject><subject>Haplotypes</subject><subject>Hogs</subject><subject>Immune system</subject><subject>Innate immunity</subject><subject>Mutation</subject><subject>NOD</subject><subject>Nod1 protein</subject><subject>NOD2 protein</subject><subject>Pathogen receptor</subject><subject>Pathogens</subject><subject>Pattern recognition</subject><subject>Pattern recognition receptors</subject><subject>Polymorphism</subject><subject>Porcine</subject><subject>Promoter</subject><subject>Proteins</subject><subject>Receptors</subject><subject>Reporter gene</subject><subject>Single-nucleotide polymorphism</subject><subject>Toll-like receptors</subject><subject>Transcription factors</subject><subject>Veterinary medicine</subject><issn>0034-5288</issn><issn>1532-2661</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNp9kT1v1TAUhi0EopfCH2BAllhYEo4_YscSC2r5qFTRBWbLcU6or5L4YieVyq_HIYWBgcm2_JxXR-9DyEsGNQOm3h7rdJd9zYGZGmQNYB6RA2sEr7hS7DE5AAhZNbxtz8iznI8AIBnTT8mZYCCAsfZAlqse5yUMwbslxJm6uafDOvvt4Ubqb11yfsEUfu7_caCnON5PMZ1uQ54yDTM9pTjFwtCMP1acPeYdSz7MSL_cXLLfseXC6XecMT8nTwY3ZnzxcJ6Tbx8_fL34XF3ffLq6eH9dedHKpdJKcyNbI1qhJKoeHQjTO88NaC26rgOnQKM2Go12GlXXOGh82xnmlGhQnJM3e27ZsGyWFzuF7HEc3YxxzZZzprTmgrcFff0PeoxrKhVslAAFEuRG8Z3yKeaccLCnFCaX7i0DuzmxR7s5sZsTC9IWJ2Xo1UP02k3Y_x35I6EA73YASxd3AZPNPmw99iGhX2wfw__yfwGWfJ1m</recordid><startdate>20190601</startdate><enddate>20190601</enddate><creator>Domínguez, Miguel A.</creator><creator>Landi, Vincenzo</creator><creator>Morera, Luis</creator><creator>Martínez, Amparo</creator><creator>Jiménez-Marín, Ángeles</creator><creator>Garrido, Juan J.</creator><general>Elsevier Ltd</general><general>Elsevier Limited</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QP</scope><scope>7QR</scope><scope>7T5</scope><scope>7T7</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-1385-8439</orcidid></search><sort><creationdate>20190601</creationdate><title>Identification and functional characterization of polymorphisms in promoter sequences of porcine NOD1 and NOD2 genes</title><author>Domínguez, Miguel A. ; Landi, Vincenzo ; Morera, Luis ; Martínez, Amparo ; Jiménez-Marín, Ángeles ; Garrido, Juan J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c384t-7672948938364e6dea039dac290773bbb0a607e797e97a7e6b5a05c8b91a635e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Bacterial infections</topic><topic>Binding sites</topic><topic>Chromosomes</topic><topic>Cytoplasm</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>Functional analysis</topic><topic>Gene expression</topic><topic>Gene sequencing</topic><topic>Genes</topic><topic>Genetic diversity</topic><topic>Genetic testing</topic><topic>Haplotypes</topic><topic>Hogs</topic><topic>Immune system</topic><topic>Innate immunity</topic><topic>Mutation</topic><topic>NOD</topic><topic>Nod1 protein</topic><topic>NOD2 protein</topic><topic>Pathogen receptor</topic><topic>Pathogens</topic><topic>Pattern recognition</topic><topic>Pattern recognition receptors</topic><topic>Polymorphism</topic><topic>Porcine</topic><topic>Promoter</topic><topic>Proteins</topic><topic>Receptors</topic><topic>Reporter gene</topic><topic>Single-nucleotide polymorphism</topic><topic>Toll-like receptors</topic><topic>Transcription factors</topic><topic>Veterinary medicine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Domínguez, Miguel A.</creatorcontrib><creatorcontrib>Landi, Vincenzo</creatorcontrib><creatorcontrib>Morera, Luis</creatorcontrib><creatorcontrib>Martínez, Amparo</creatorcontrib><creatorcontrib>Jiménez-Marín, Ángeles</creatorcontrib><creatorcontrib>Garrido, Juan J.</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Animal Behavior Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Research in veterinary science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Domínguez, Miguel A.</au><au>Landi, Vincenzo</au><au>Morera, Luis</au><au>Martínez, Amparo</au><au>Jiménez-Marín, Ángeles</au><au>Garrido, Juan J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification and functional characterization of polymorphisms in promoter sequences of porcine NOD1 and NOD2 genes</atitle><jtitle>Research in veterinary science</jtitle><addtitle>Res Vet Sci</addtitle><date>2019-06-01</date><risdate>2019</risdate><volume>124</volume><spage>310</spage><epage>316</epage><pages>310-316</pages><issn>0034-5288</issn><eissn>1532-2661</eissn><abstract>NOD-like receptors (NLRs) play a key role in the innate immune system, acting as a second line of surveillance against pathogens. NLRs detect particular bacteria that have gained access to the cytoplasm, evading recognition by other pattern recognition receptors, such as Toll-like receptors. It has been demonstrated that coding sequence-single nucleotide polymorphisms may alter the ligand recognition ability of NLRs, affecting their pathogen-sensing function. However, there have been no data relating to the identification and functional analysis of SNPs in porcine NLR promoters. We examined the promoter sequences of the porcine NOD1 and NOD2 genes with the aim to identify and to evaluate the effect of genetic variations on promoter activity. Six SNPs in NOD1 and three SNPs in NOD2 were identified. Luciferase reporter gene assays showed significant differences in promoter activity between allele variants of NOD1 -920G>A (NC_010460.4:g.42431413G>A) and NOD2 -1670G>A (NC_010448.4:g.34169122T>C) SNPs. The results suggest that promoter polymorphisms could modify the expression levels of porcine NOD1 and NOD2 genes.
•NOD genes play a critical role in initiating the development of immune response•Analysis of SNP will expand the knowledge of genetic resistance to pathogens•Promoter polymorphisms modify the expression levels of porcine NOD genes</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>31030118</pmid><doi>10.1016/j.rvsc.2019.04.009</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0003-1385-8439</orcidid></addata></record> |
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| subjects | Bacterial infections Binding sites Chromosomes Cytoplasm Deoxyribonucleic acid DNA Functional analysis Gene expression Gene sequencing Genes Genetic diversity Genetic testing Haplotypes Hogs Immune system Innate immunity Mutation NOD Nod1 protein NOD2 protein Pathogen receptor Pathogens Pattern recognition Pattern recognition receptors Polymorphism Porcine Promoter Proteins Receptors Reporter gene Single-nucleotide polymorphism Toll-like receptors Transcription factors Veterinary medicine |
| title | Identification and functional characterization of polymorphisms in promoter sequences of porcine NOD1 and NOD2 genes |
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