Inhibitory effects of sildenafil and tadalafil on inflammation, oxidative stress and nitrosative stress in animal model of bronchial asthma
Cyclic neucleotides are involved in many cellular functions including smooth muscle relaxation, inflammation, and signal transduction. Sildenafil and tadalafil are phosphodiesterase-5 (PDE-5) inhibitors which prevent the degradation of cyclic neucleotide i.e. guanosine 3′,5′ cyclic monophosphate (cG...
Gespeichert in:
| Veröffentlicht in: | Pharmacological reports 2019-06, Vol.71 (3), p.517-521 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 521 |
|---|---|
| container_issue | 3 |
| container_start_page | 517 |
| container_title | Pharmacological reports |
| container_volume | 71 |
| creator | Laxmi, Vijaya Gupta, Rachna Bhattacharya, Swapan K. Ray, Arunabha Gulati, Kavita |
| description | Cyclic neucleotides are involved in many cellular functions including smooth muscle relaxation, inflammation, and signal transduction. Sildenafil and tadalafil are phosphodiesterase-5 (PDE-5) inhibitors which prevent the degradation of cyclic neucleotide i.e. guanosine 3′,5′ cyclic monophosphate (cGMP) and increase the levels of cGMP. In this study sildenafil and tadalafil were evaluated for their anti-inflammatory, anti-oxidative and anti-nitrosative stress potential in animal model of bronchial asthma.
Wistar rats were sensitized with 10 mg intraperitoneal (ip) ovalbumin adsorbed to 10 μg of aluminum hydroxide on day 0. Animals were given sildenafil (1 and 3 mg/kg ip) and tadalafil (1 and 3 mg/kg ip) from day 1 to day 14. Also, on day 14 animals were challenged with ovalbumin (1 mg ip). After 24 h, samples were collected to analyze interleukin-4 (IL-4) and tumour necrosis factor-α (TNF-α), in serum and bronchoalveolar lavage fluid (BALF). The oxidative stress markers malondialdehyde (MDA), reduced glutathione (GSH) and nitric oxide metabolites (NOx) were also measured in serum.
Pre-treatment with sildenafil (1 and 3 mg/kg ip) and tadalafil (1 and 3 mg/kg ip) significantly reduced the levels of pro-inflammatory cytokines IL-4 and TNF-α in rat serum and BALF. In addition, pre-treatment with both the drugs decreased the levels of MDA and NOx and increased the levels of GSH in serum.
Sildenafil and tadalafil decreased pro-inflammatory cytokines in serum and BALF. Both drugs inhibit oxidative and nitrosative stress in animal model of bronchial asthma and could have a therapeutic potential in bronchial asthma. |
| doi_str_mv | 10.1016/j.pharep.2019.02.008 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2213154614</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S1734114018303487</els_id><sourcerecordid>2213154614</sourcerecordid><originalsourceid>FETCH-LOGICAL-c408t-cb3471f48e69078f0a785293c28fdf921ba8ea40c148cc630d0794692f1f7e63</originalsourceid><addsrcrecordid>eNqFUcFu1DAQtRCo3Zb-AUI5ciDp2HES54KEKqCVKvXSu-XYY9Yrx17sbEW_oT-NtylIXODk8cx7b_TmEfKOQkOB9pe7Zr9VCfcNAzo2wBoA8YpsGBvHuusFf002dGh5TSmHU3KW8w6AU9Z2J-S0pQCj4O2GPN2ErZvcEtNjhdaiXnIVbZWdNxiUdb5SwVSLMso__2KoXLBezbNaXAwfq_jTmVI-YJWXhDk_44NbUsx_tV0oEzcrX83RoD8umVIMeutKS-VlO6u35I1VPuPFy3tO7r9-ub-6rm_vvt1cfb6tNQex1Hpq-UAtF9iPMAgLahAdG1vNhDV2ZHRSAhUHTbnQum_BwDDyfmSW2gH79px8WGX3Kf44YF7k7LJG71XAeMiSMdrSjveUFyhfobrYyQmt3KfiIT1KCvKYgtzJNQV5TEECkyWFQnv_suEwzWj-kH6fvQC6FZDLKHzHJHfxkEIx_T_hTysPy3keXOFl7TBoNC6V6KSJ7t8CvwBe-a7V</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2213154614</pqid></control><display><type>article</type><title>Inhibitory effects of sildenafil and tadalafil on inflammation, oxidative stress and nitrosative stress in animal model of bronchial asthma</title><source>MEDLINE</source><source>SpringerLink (Online service)</source><creator>Laxmi, Vijaya ; Gupta, Rachna ; Bhattacharya, Swapan K. ; Ray, Arunabha ; Gulati, Kavita</creator><creatorcontrib>Laxmi, Vijaya ; Gupta, Rachna ; Bhattacharya, Swapan K. ; Ray, Arunabha ; Gulati, Kavita</creatorcontrib><description>Cyclic neucleotides are involved in many cellular functions including smooth muscle relaxation, inflammation, and signal transduction. Sildenafil and tadalafil are phosphodiesterase-5 (PDE-5) inhibitors which prevent the degradation of cyclic neucleotide i.e. guanosine 3′,5′ cyclic monophosphate (cGMP) and increase the levels of cGMP. In this study sildenafil and tadalafil were evaluated for their anti-inflammatory, anti-oxidative and anti-nitrosative stress potential in animal model of bronchial asthma.
Wistar rats were sensitized with 10 mg intraperitoneal (ip) ovalbumin adsorbed to 10 μg of aluminum hydroxide on day 0. Animals were given sildenafil (1 and 3 mg/kg ip) and tadalafil (1 and 3 mg/kg ip) from day 1 to day 14. Also, on day 14 animals were challenged with ovalbumin (1 mg ip). After 24 h, samples were collected to analyze interleukin-4 (IL-4) and tumour necrosis factor-α (TNF-α), in serum and bronchoalveolar lavage fluid (BALF). The oxidative stress markers malondialdehyde (MDA), reduced glutathione (GSH) and nitric oxide metabolites (NOx) were also measured in serum.
Pre-treatment with sildenafil (1 and 3 mg/kg ip) and tadalafil (1 and 3 mg/kg ip) significantly reduced the levels of pro-inflammatory cytokines IL-4 and TNF-α in rat serum and BALF. In addition, pre-treatment with both the drugs decreased the levels of MDA and NOx and increased the levels of GSH in serum.
Sildenafil and tadalafil decreased pro-inflammatory cytokines in serum and BALF. Both drugs inhibit oxidative and nitrosative stress in animal model of bronchial asthma and could have a therapeutic potential in bronchial asthma.</description><identifier>ISSN: 1734-1140</identifier><identifier>EISSN: 2299-5684</identifier><identifier>DOI: 10.1016/j.pharep.2019.02.008</identifier><identifier>PMID: 31009843</identifier><language>eng</language><publisher>Cham: Elsevier B.V</publisher><subject>Animals ; Asthma - drug therapy ; Asthma - metabolism ; Bronchi - drug effects ; Bronchi - metabolism ; Bronchial asthma ; Bronchoalveolar Lavage Fluid - chemistry ; Cytokines - metabolism ; Disease Models, Animal ; Drug Safety and Pharmacovigilance ; Female ; Glutathione - metabolism ; Inflammation ; Inflammation - drug therapy ; Inflammation - metabolism ; Lung - drug effects ; Lung - metabolism ; Male ; Malondialdehyde - metabolism ; Nitric Oxide - metabolism ; Nitrosative Stress - drug effects ; Original Article ; Ovalbumin ; Oxidative stress ; Oxidative Stress - drug effects ; Pharmacotherapy ; Pharmacy ; Rats ; Rats, Wistar ; Sildenafil ; Sildenafil Citrate - pharmacology ; Tadalafil ; Tadalafil - pharmacology</subject><ispartof>Pharmacological reports, 2019-06, Vol.71 (3), p.517-521</ispartof><rights>2019 Institute of Pharmacology, Polish Academy of Sciences</rights><rights>Maj Institute of Pharmacology Polish Academy of Sciences 2019</rights><rights>Copyright © 2019 Institute of Pharmacology, Polish Academy of Sciences. Published by Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c408t-cb3471f48e69078f0a785293c28fdf921ba8ea40c148cc630d0794692f1f7e63</citedby><cites>FETCH-LOGICAL-c408t-cb3471f48e69078f0a785293c28fdf921ba8ea40c148cc630d0794692f1f7e63</cites><orcidid>0000-0001-6496-8617</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1016/j.pharep.2019.02.008$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1016/j.pharep.2019.02.008$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,777,781,27905,27906,41469,42538,51300</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31009843$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Laxmi, Vijaya</creatorcontrib><creatorcontrib>Gupta, Rachna</creatorcontrib><creatorcontrib>Bhattacharya, Swapan K.</creatorcontrib><creatorcontrib>Ray, Arunabha</creatorcontrib><creatorcontrib>Gulati, Kavita</creatorcontrib><title>Inhibitory effects of sildenafil and tadalafil on inflammation, oxidative stress and nitrosative stress in animal model of bronchial asthma</title><title>Pharmacological reports</title><addtitle>Pharmacol. Rep</addtitle><addtitle>Pharmacol Rep</addtitle><description>Cyclic neucleotides are involved in many cellular functions including smooth muscle relaxation, inflammation, and signal transduction. Sildenafil and tadalafil are phosphodiesterase-5 (PDE-5) inhibitors which prevent the degradation of cyclic neucleotide i.e. guanosine 3′,5′ cyclic monophosphate (cGMP) and increase the levels of cGMP. In this study sildenafil and tadalafil were evaluated for their anti-inflammatory, anti-oxidative and anti-nitrosative stress potential in animal model of bronchial asthma.
Wistar rats were sensitized with 10 mg intraperitoneal (ip) ovalbumin adsorbed to 10 μg of aluminum hydroxide on day 0. Animals were given sildenafil (1 and 3 mg/kg ip) and tadalafil (1 and 3 mg/kg ip) from day 1 to day 14. Also, on day 14 animals were challenged with ovalbumin (1 mg ip). After 24 h, samples were collected to analyze interleukin-4 (IL-4) and tumour necrosis factor-α (TNF-α), in serum and bronchoalveolar lavage fluid (BALF). The oxidative stress markers malondialdehyde (MDA), reduced glutathione (GSH) and nitric oxide metabolites (NOx) were also measured in serum.
Pre-treatment with sildenafil (1 and 3 mg/kg ip) and tadalafil (1 and 3 mg/kg ip) significantly reduced the levels of pro-inflammatory cytokines IL-4 and TNF-α in rat serum and BALF. In addition, pre-treatment with both the drugs decreased the levels of MDA and NOx and increased the levels of GSH in serum.
Sildenafil and tadalafil decreased pro-inflammatory cytokines in serum and BALF. Both drugs inhibit oxidative and nitrosative stress in animal model of bronchial asthma and could have a therapeutic potential in bronchial asthma.</description><subject>Animals</subject><subject>Asthma - drug therapy</subject><subject>Asthma - metabolism</subject><subject>Bronchi - drug effects</subject><subject>Bronchi - metabolism</subject><subject>Bronchial asthma</subject><subject>Bronchoalveolar Lavage Fluid - chemistry</subject><subject>Cytokines - metabolism</subject><subject>Disease Models, Animal</subject><subject>Drug Safety and Pharmacovigilance</subject><subject>Female</subject><subject>Glutathione - metabolism</subject><subject>Inflammation</subject><subject>Inflammation - drug therapy</subject><subject>Inflammation - metabolism</subject><subject>Lung - drug effects</subject><subject>Lung - metabolism</subject><subject>Male</subject><subject>Malondialdehyde - metabolism</subject><subject>Nitric Oxide - metabolism</subject><subject>Nitrosative Stress - drug effects</subject><subject>Original Article</subject><subject>Ovalbumin</subject><subject>Oxidative stress</subject><subject>Oxidative Stress - drug effects</subject><subject>Pharmacotherapy</subject><subject>Pharmacy</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Sildenafil</subject><subject>Sildenafil Citrate - pharmacology</subject><subject>Tadalafil</subject><subject>Tadalafil - pharmacology</subject><issn>1734-1140</issn><issn>2299-5684</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFUcFu1DAQtRCo3Zb-AUI5ciDp2HES54KEKqCVKvXSu-XYY9Yrx17sbEW_oT-NtylIXODk8cx7b_TmEfKOQkOB9pe7Zr9VCfcNAzo2wBoA8YpsGBvHuusFf002dGh5TSmHU3KW8w6AU9Z2J-S0pQCj4O2GPN2ErZvcEtNjhdaiXnIVbZWdNxiUdb5SwVSLMso__2KoXLBezbNaXAwfq_jTmVI-YJWXhDk_44NbUsx_tV0oEzcrX83RoD8umVIMeutKS-VlO6u35I1VPuPFy3tO7r9-ub-6rm_vvt1cfb6tNQex1Hpq-UAtF9iPMAgLahAdG1vNhDV2ZHRSAhUHTbnQum_BwDDyfmSW2gH79px8WGX3Kf44YF7k7LJG71XAeMiSMdrSjveUFyhfobrYyQmt3KfiIT1KCvKYgtzJNQV5TEECkyWFQnv_suEwzWj-kH6fvQC6FZDLKHzHJHfxkEIx_T_hTysPy3keXOFl7TBoNC6V6KSJ7t8CvwBe-a7V</recordid><startdate>20190601</startdate><enddate>20190601</enddate><creator>Laxmi, Vijaya</creator><creator>Gupta, Rachna</creator><creator>Bhattacharya, Swapan K.</creator><creator>Ray, Arunabha</creator><creator>Gulati, Kavita</creator><general>Elsevier B.V</general><general>Springer International Publishing</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-6496-8617</orcidid></search><sort><creationdate>20190601</creationdate><title>Inhibitory effects of sildenafil and tadalafil on inflammation, oxidative stress and nitrosative stress in animal model of bronchial asthma</title><author>Laxmi, Vijaya ; Gupta, Rachna ; Bhattacharya, Swapan K. ; Ray, Arunabha ; Gulati, Kavita</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c408t-cb3471f48e69078f0a785293c28fdf921ba8ea40c148cc630d0794692f1f7e63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Animals</topic><topic>Asthma - drug therapy</topic><topic>Asthma - metabolism</topic><topic>Bronchi - drug effects</topic><topic>Bronchi - metabolism</topic><topic>Bronchial asthma</topic><topic>Bronchoalveolar Lavage Fluid - chemistry</topic><topic>Cytokines - metabolism</topic><topic>Disease Models, Animal</topic><topic>Drug Safety and Pharmacovigilance</topic><topic>Female</topic><topic>Glutathione - metabolism</topic><topic>Inflammation</topic><topic>Inflammation - drug therapy</topic><topic>Inflammation - metabolism</topic><topic>Lung - drug effects</topic><topic>Lung - metabolism</topic><topic>Male</topic><topic>Malondialdehyde - metabolism</topic><topic>Nitric Oxide - metabolism</topic><topic>Nitrosative Stress - drug effects</topic><topic>Original Article</topic><topic>Ovalbumin</topic><topic>Oxidative stress</topic><topic>Oxidative Stress - drug effects</topic><topic>Pharmacotherapy</topic><topic>Pharmacy</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Sildenafil</topic><topic>Sildenafil Citrate - pharmacology</topic><topic>Tadalafil</topic><topic>Tadalafil - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Laxmi, Vijaya</creatorcontrib><creatorcontrib>Gupta, Rachna</creatorcontrib><creatorcontrib>Bhattacharya, Swapan K.</creatorcontrib><creatorcontrib>Ray, Arunabha</creatorcontrib><creatorcontrib>Gulati, Kavita</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Pharmacological reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Laxmi, Vijaya</au><au>Gupta, Rachna</au><au>Bhattacharya, Swapan K.</au><au>Ray, Arunabha</au><au>Gulati, Kavita</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Inhibitory effects of sildenafil and tadalafil on inflammation, oxidative stress and nitrosative stress in animal model of bronchial asthma</atitle><jtitle>Pharmacological reports</jtitle><stitle>Pharmacol. Rep</stitle><addtitle>Pharmacol Rep</addtitle><date>2019-06-01</date><risdate>2019</risdate><volume>71</volume><issue>3</issue><spage>517</spage><epage>521</epage><pages>517-521</pages><issn>1734-1140</issn><eissn>2299-5684</eissn><abstract>Cyclic neucleotides are involved in many cellular functions including smooth muscle relaxation, inflammation, and signal transduction. Sildenafil and tadalafil are phosphodiesterase-5 (PDE-5) inhibitors which prevent the degradation of cyclic neucleotide i.e. guanosine 3′,5′ cyclic monophosphate (cGMP) and increase the levels of cGMP. In this study sildenafil and tadalafil were evaluated for their anti-inflammatory, anti-oxidative and anti-nitrosative stress potential in animal model of bronchial asthma.
Wistar rats were sensitized with 10 mg intraperitoneal (ip) ovalbumin adsorbed to 10 μg of aluminum hydroxide on day 0. Animals were given sildenafil (1 and 3 mg/kg ip) and tadalafil (1 and 3 mg/kg ip) from day 1 to day 14. Also, on day 14 animals were challenged with ovalbumin (1 mg ip). After 24 h, samples were collected to analyze interleukin-4 (IL-4) and tumour necrosis factor-α (TNF-α), in serum and bronchoalveolar lavage fluid (BALF). The oxidative stress markers malondialdehyde (MDA), reduced glutathione (GSH) and nitric oxide metabolites (NOx) were also measured in serum.
Pre-treatment with sildenafil (1 and 3 mg/kg ip) and tadalafil (1 and 3 mg/kg ip) significantly reduced the levels of pro-inflammatory cytokines IL-4 and TNF-α in rat serum and BALF. In addition, pre-treatment with both the drugs decreased the levels of MDA and NOx and increased the levels of GSH in serum.
Sildenafil and tadalafil decreased pro-inflammatory cytokines in serum and BALF. Both drugs inhibit oxidative and nitrosative stress in animal model of bronchial asthma and could have a therapeutic potential in bronchial asthma.</abstract><cop>Cham</cop><pub>Elsevier B.V</pub><pmid>31009843</pmid><doi>10.1016/j.pharep.2019.02.008</doi><tpages>5</tpages><orcidid>https://orcid.org/0000-0001-6496-8617</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1734-1140 |
| ispartof | Pharmacological reports, 2019-06, Vol.71 (3), p.517-521 |
| issn | 1734-1140 2299-5684 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2213154614 |
| source | MEDLINE; SpringerLink (Online service) |
| subjects | Animals Asthma - drug therapy Asthma - metabolism Bronchi - drug effects Bronchi - metabolism Bronchial asthma Bronchoalveolar Lavage Fluid - chemistry Cytokines - metabolism Disease Models, Animal Drug Safety and Pharmacovigilance Female Glutathione - metabolism Inflammation Inflammation - drug therapy Inflammation - metabolism Lung - drug effects Lung - metabolism Male Malondialdehyde - metabolism Nitric Oxide - metabolism Nitrosative Stress - drug effects Original Article Ovalbumin Oxidative stress Oxidative Stress - drug effects Pharmacotherapy Pharmacy Rats Rats, Wistar Sildenafil Sildenafil Citrate - pharmacology Tadalafil Tadalafil - pharmacology |
| title | Inhibitory effects of sildenafil and tadalafil on inflammation, oxidative stress and nitrosative stress in animal model of bronchial asthma |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-17T19%3A10%3A53IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Inhibitory%20effects%20of%20sildenafil%20and%20tadalafil%20on%20inflammation,%20oxidative%20stress%20and%20nitrosative%20stress%20in%20animal%20model%20of%20bronchial%20asthma&rft.jtitle=Pharmacological%20reports&rft.au=Laxmi,%20Vijaya&rft.date=2019-06-01&rft.volume=71&rft.issue=3&rft.spage=517&rft.epage=521&rft.pages=517-521&rft.issn=1734-1140&rft.eissn=2299-5684&rft_id=info:doi/10.1016/j.pharep.2019.02.008&rft_dat=%3Cproquest_cross%3E2213154614%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2213154614&rft_id=info:pmid/31009843&rft_els_id=S1734114018303487&rfr_iscdi=true |