Fate of Mycobacterium avium subsp. paratuberculosis and changes in bacterial diversity populations in dairy slurry after chemical treatments
Aims A major drawback of using dairy slurry as fertilizer is that it may contains pathogens such as Mycobacterium avium subsp. paratuberculosis (MAP), and it could represent a risk to animal and public health. Thus, the aim of this study was to evaluate the fate of MAP and bacterial communities in d...
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| Veröffentlicht in: | Journal of applied microbiology 2019-08, Vol.127 (2), p.370-378 |
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| container_title | Journal of applied microbiology |
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| creator | Avilez, C. Alfaro, M.A. Salazar, F. Encina, C. Verdugo, C. Martínez, O. Collins, M.T. Salgado, M. |
| description | Aims
A major drawback of using dairy slurry as fertilizer is that it may contains pathogens such as Mycobacterium avium subsp. paratuberculosis (MAP), and it could represent a risk to animal and public health. Thus, the aim of this study was to evaluate the fate of MAP and bacterial communities in dairy slurry after chemical treatments.
Methods and Results
Cattle slurry, naturally contaminated with MAP, was collected from a dairy herd and divided into 32 glass bottles which were assigned to eight different treatments (control, 3·0% CaO, 0·5% NaOH; 0·087%, 0·11% and 0·14% H2SO4; and 1·0 and 2·5% KMnO4). Treated dairy slurry samples were evaluated at 0, 1, 3, 7, 15, 30 and 60‐days following treatment application for viable MAP and dairy slurry pH, and in addition temperature in this material was monitored continuously. Bacterial counts were estimated at each sampling time. A Bayesian zero‐inflated Poisson mixed model was fitted to assess the effect of each treatment on the count of MAP cells. Model results indicated that only the 3·0% CaO treatment had a statistically important negative effect on MAP counts during the study period. For most treatments, MAP was undetectable immediately after chemical treatment but re‐appeared over time, in some replicates at low concentrations. However, in those cases MAP counts were not statistically different than the control treatment. Regarding the fate of the other bacterial populations, the Firmicutes phylum was the dominant population in the un‐treated slurry while Clostridia class members were among the most prevalent bacteria after the application of most chemical treatments.
Conclusion
Only 3% CaO treatment had a statistically important negative effect on MAP viability in cattle slurry.
Significance and Impact of the Study
This study provides evidence of MAP partial control in dairy slurry. This information should be considered as a best management practice to reduce MAP and other pathogens for slurry management on dairy farms. |
| doi_str_mv | 10.1111/jam.14288 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2212719546</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2212719546</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3538-8f6a3ea383aae27fce71324f2fc4e7f5cf81d642535f050979c9f4e97ccebe853</originalsourceid><addsrcrecordid>eNp10ctu1DAUBmALgWgpLHgBZIkNLDL1JY6dZVVRLmrFBtbRiXMMHiVxsOOivAMPjTszsEDCCx8vvvPL0k_IS852vJzLPUw7XgtjHpFzLhtViUaLx4d3XSmmxRl5ltKeMS6Zap6SM8kZa3mtz8mvG1iRBkfvNht6sCtGnycK9w93yn1adnSBCGvuMdo8huQThXmg9jvM3zBRP9PTGox08PcYk183uoQlj7D6MB_IAD5uNI05lgGu8BKAk7dlaY0I64Tzmp6TJw7GhC9O84J8vXn35fpDdfv5_cfrq9vKSiVNZVwDEkEaCYBCO4uaS1E74WyN2inrDB-aWiipHFOs1a1tXY2tthZ7NEpekDfH3CWGHxnT2k0-WRxHmDHk1AnBheatqptCX_9D9yHHufyuKCVa02pTF_X2qGwMKUV03RL9BHHrOOseKupKRd2homJfnRJzP-HwV_7ppIDLI_jpR9z-n9R9uro7Rv4GwMad0g</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2252989784</pqid></control><display><type>article</type><title>Fate of Mycobacterium avium subsp. paratuberculosis and changes in bacterial diversity populations in dairy slurry after chemical treatments</title><source>Wiley Online Library - AutoHoldings Journals</source><source>MEDLINE</source><source>Oxford University Press Journals All Titles (1996-Current)</source><creator>Avilez, C. ; Alfaro, M.A. ; Salazar, F. ; Encina, C. ; Verdugo, C. ; Martínez, O. ; Collins, M.T. ; Salgado, M.</creator><creatorcontrib>Avilez, C. ; Alfaro, M.A. ; Salazar, F. ; Encina, C. ; Verdugo, C. ; Martínez, O. ; Collins, M.T. ; Salgado, M.</creatorcontrib><description>Aims
A major drawback of using dairy slurry as fertilizer is that it may contains pathogens such as Mycobacterium avium subsp. paratuberculosis (MAP), and it could represent a risk to animal and public health. Thus, the aim of this study was to evaluate the fate of MAP and bacterial communities in dairy slurry after chemical treatments.
Methods and Results
Cattle slurry, naturally contaminated with MAP, was collected from a dairy herd and divided into 32 glass bottles which were assigned to eight different treatments (control, 3·0% CaO, 0·5% NaOH; 0·087%, 0·11% and 0·14% H2SO4; and 1·0 and 2·5% KMnO4). Treated dairy slurry samples were evaluated at 0, 1, 3, 7, 15, 30 and 60‐days following treatment application for viable MAP and dairy slurry pH, and in addition temperature in this material was monitored continuously. Bacterial counts were estimated at each sampling time. A Bayesian zero‐inflated Poisson mixed model was fitted to assess the effect of each treatment on the count of MAP cells. Model results indicated that only the 3·0% CaO treatment had a statistically important negative effect on MAP counts during the study period. For most treatments, MAP was undetectable immediately after chemical treatment but re‐appeared over time, in some replicates at low concentrations. However, in those cases MAP counts were not statistically different than the control treatment. Regarding the fate of the other bacterial populations, the Firmicutes phylum was the dominant population in the un‐treated slurry while Clostridia class members were among the most prevalent bacteria after the application of most chemical treatments.
Conclusion
Only 3% CaO treatment had a statistically important negative effect on MAP viability in cattle slurry.
Significance and Impact of the Study
This study provides evidence of MAP partial control in dairy slurry. This information should be considered as a best management practice to reduce MAP and other pathogens for slurry management on dairy farms.</description><identifier>ISSN: 1364-5072</identifier><identifier>EISSN: 1365-2672</identifier><identifier>DOI: 10.1111/jam.14288</identifier><identifier>PMID: 31009147</identifier><language>eng</language><publisher>England: Oxford University Press</publisher><subject>Animals ; Anti-Bacterial Agents - pharmacology ; Bacteria ; Bayes Theorem ; Bayesian analysis ; Best management practices ; Calcium Compounds - pharmacology ; Cattle ; Cattle manure ; Chemical treatment ; Dairy cattle ; Dairy farms ; dairy slurry ; Dairying ; Farm management ; Farms ; Female ; Fertilizers ; Firmicutes ; Low concentrations ; Manure - microbiology ; Mycobacterium avium ; Mycobacterium avium subsp. paratuberculosis - drug effects ; Mycobacterium avium subsp. paratuberculosis - isolation & purification ; Mycobacterium avium subspecies paratuberculosis ; Organic chemistry ; Oxides - pharmacology ; Paratuberculosis ; Pathogens ; Populations ; Potassium permanganate ; Public health ; Slurries ; Sodium hydroxide ; Statistical methods ; Sulfuric acid ; Viability</subject><ispartof>Journal of applied microbiology, 2019-08, Vol.127 (2), p.370-378</ispartof><rights>2019 The Society for Applied Microbiology</rights><rights>2019 The Society for Applied Microbiology.</rights><rights>Copyright © 2019 The Society for Applied Microbiology</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3538-8f6a3ea383aae27fce71324f2fc4e7f5cf81d642535f050979c9f4e97ccebe853</citedby><cites>FETCH-LOGICAL-c3538-8f6a3ea383aae27fce71324f2fc4e7f5cf81d642535f050979c9f4e97ccebe853</cites><orcidid>0000-0002-2144-7982</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fjam.14288$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fjam.14288$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27903,27904,45553,45554</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31009147$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Avilez, C.</creatorcontrib><creatorcontrib>Alfaro, M.A.</creatorcontrib><creatorcontrib>Salazar, F.</creatorcontrib><creatorcontrib>Encina, C.</creatorcontrib><creatorcontrib>Verdugo, C.</creatorcontrib><creatorcontrib>Martínez, O.</creatorcontrib><creatorcontrib>Collins, M.T.</creatorcontrib><creatorcontrib>Salgado, M.</creatorcontrib><title>Fate of Mycobacterium avium subsp. paratuberculosis and changes in bacterial diversity populations in dairy slurry after chemical treatments</title><title>Journal of applied microbiology</title><addtitle>J Appl Microbiol</addtitle><description>Aims
A major drawback of using dairy slurry as fertilizer is that it may contains pathogens such as Mycobacterium avium subsp. paratuberculosis (MAP), and it could represent a risk to animal and public health. Thus, the aim of this study was to evaluate the fate of MAP and bacterial communities in dairy slurry after chemical treatments.
Methods and Results
Cattle slurry, naturally contaminated with MAP, was collected from a dairy herd and divided into 32 glass bottles which were assigned to eight different treatments (control, 3·0% CaO, 0·5% NaOH; 0·087%, 0·11% and 0·14% H2SO4; and 1·0 and 2·5% KMnO4). Treated dairy slurry samples were evaluated at 0, 1, 3, 7, 15, 30 and 60‐days following treatment application for viable MAP and dairy slurry pH, and in addition temperature in this material was monitored continuously. Bacterial counts were estimated at each sampling time. A Bayesian zero‐inflated Poisson mixed model was fitted to assess the effect of each treatment on the count of MAP cells. Model results indicated that only the 3·0% CaO treatment had a statistically important negative effect on MAP counts during the study period. For most treatments, MAP was undetectable immediately after chemical treatment but re‐appeared over time, in some replicates at low concentrations. However, in those cases MAP counts were not statistically different than the control treatment. Regarding the fate of the other bacterial populations, the Firmicutes phylum was the dominant population in the un‐treated slurry while Clostridia class members were among the most prevalent bacteria after the application of most chemical treatments.
Conclusion
Only 3% CaO treatment had a statistically important negative effect on MAP viability in cattle slurry.
Significance and Impact of the Study
This study provides evidence of MAP partial control in dairy slurry. This information should be considered as a best management practice to reduce MAP and other pathogens for slurry management on dairy farms.</description><subject>Animals</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Bacteria</subject><subject>Bayes Theorem</subject><subject>Bayesian analysis</subject><subject>Best management practices</subject><subject>Calcium Compounds - pharmacology</subject><subject>Cattle</subject><subject>Cattle manure</subject><subject>Chemical treatment</subject><subject>Dairy cattle</subject><subject>Dairy farms</subject><subject>dairy slurry</subject><subject>Dairying</subject><subject>Farm management</subject><subject>Farms</subject><subject>Female</subject><subject>Fertilizers</subject><subject>Firmicutes</subject><subject>Low concentrations</subject><subject>Manure - microbiology</subject><subject>Mycobacterium avium</subject><subject>Mycobacterium avium subsp. paratuberculosis - drug effects</subject><subject>Mycobacterium avium subsp. paratuberculosis - isolation & purification</subject><subject>Mycobacterium avium subspecies paratuberculosis</subject><subject>Organic chemistry</subject><subject>Oxides - pharmacology</subject><subject>Paratuberculosis</subject><subject>Pathogens</subject><subject>Populations</subject><subject>Potassium permanganate</subject><subject>Public health</subject><subject>Slurries</subject><subject>Sodium hydroxide</subject><subject>Statistical methods</subject><subject>Sulfuric acid</subject><subject>Viability</subject><issn>1364-5072</issn><issn>1365-2672</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp10ctu1DAUBmALgWgpLHgBZIkNLDL1JY6dZVVRLmrFBtbRiXMMHiVxsOOivAMPjTszsEDCCx8vvvPL0k_IS852vJzLPUw7XgtjHpFzLhtViUaLx4d3XSmmxRl5ltKeMS6Zap6SM8kZa3mtz8mvG1iRBkfvNht6sCtGnycK9w93yn1adnSBCGvuMdo8huQThXmg9jvM3zBRP9PTGox08PcYk183uoQlj7D6MB_IAD5uNI05lgGu8BKAk7dlaY0I64Tzmp6TJw7GhC9O84J8vXn35fpDdfv5_cfrq9vKSiVNZVwDEkEaCYBCO4uaS1E74WyN2inrDB-aWiipHFOs1a1tXY2tthZ7NEpekDfH3CWGHxnT2k0-WRxHmDHk1AnBheatqptCX_9D9yHHufyuKCVa02pTF_X2qGwMKUV03RL9BHHrOOseKupKRd2homJfnRJzP-HwV_7ppIDLI_jpR9z-n9R9uro7Rv4GwMad0g</recordid><startdate>201908</startdate><enddate>201908</enddate><creator>Avilez, C.</creator><creator>Alfaro, M.A.</creator><creator>Salazar, F.</creator><creator>Encina, C.</creator><creator>Verdugo, C.</creator><creator>Martínez, O.</creator><creator>Collins, M.T.</creator><creator>Salgado, M.</creator><general>Oxford University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>7TM</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-2144-7982</orcidid></search><sort><creationdate>201908</creationdate><title>Fate of Mycobacterium avium subsp. paratuberculosis and changes in bacterial diversity populations in dairy slurry after chemical treatments</title><author>Avilez, C. ; Alfaro, M.A. ; Salazar, F. ; Encina, C. ; Verdugo, C. ; Martínez, O. ; Collins, M.T. ; Salgado, M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3538-8f6a3ea383aae27fce71324f2fc4e7f5cf81d642535f050979c9f4e97ccebe853</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Animals</topic><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Bacteria</topic><topic>Bayes Theorem</topic><topic>Bayesian analysis</topic><topic>Best management practices</topic><topic>Calcium Compounds - pharmacology</topic><topic>Cattle</topic><topic>Cattle manure</topic><topic>Chemical treatment</topic><topic>Dairy cattle</topic><topic>Dairy farms</topic><topic>dairy slurry</topic><topic>Dairying</topic><topic>Farm management</topic><topic>Farms</topic><topic>Female</topic><topic>Fertilizers</topic><topic>Firmicutes</topic><topic>Low concentrations</topic><topic>Manure - microbiology</topic><topic>Mycobacterium avium</topic><topic>Mycobacterium avium subsp. paratuberculosis - drug effects</topic><topic>Mycobacterium avium subsp. paratuberculosis - isolation & purification</topic><topic>Mycobacterium avium subspecies paratuberculosis</topic><topic>Organic chemistry</topic><topic>Oxides - pharmacology</topic><topic>Paratuberculosis</topic><topic>Pathogens</topic><topic>Populations</topic><topic>Potassium permanganate</topic><topic>Public health</topic><topic>Slurries</topic><topic>Sodium hydroxide</topic><topic>Statistical methods</topic><topic>Sulfuric acid</topic><topic>Viability</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Avilez, C.</creatorcontrib><creatorcontrib>Alfaro, M.A.</creatorcontrib><creatorcontrib>Salazar, F.</creatorcontrib><creatorcontrib>Encina, C.</creatorcontrib><creatorcontrib>Verdugo, C.</creatorcontrib><creatorcontrib>Martínez, O.</creatorcontrib><creatorcontrib>Collins, M.T.</creatorcontrib><creatorcontrib>Salgado, M.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of applied microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Avilez, C.</au><au>Alfaro, M.A.</au><au>Salazar, F.</au><au>Encina, C.</au><au>Verdugo, C.</au><au>Martínez, O.</au><au>Collins, M.T.</au><au>Salgado, M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Fate of Mycobacterium avium subsp. paratuberculosis and changes in bacterial diversity populations in dairy slurry after chemical treatments</atitle><jtitle>Journal of applied microbiology</jtitle><addtitle>J Appl Microbiol</addtitle><date>2019-08</date><risdate>2019</risdate><volume>127</volume><issue>2</issue><spage>370</spage><epage>378</epage><pages>370-378</pages><issn>1364-5072</issn><eissn>1365-2672</eissn><abstract>Aims
A major drawback of using dairy slurry as fertilizer is that it may contains pathogens such as Mycobacterium avium subsp. paratuberculosis (MAP), and it could represent a risk to animal and public health. Thus, the aim of this study was to evaluate the fate of MAP and bacterial communities in dairy slurry after chemical treatments.
Methods and Results
Cattle slurry, naturally contaminated with MAP, was collected from a dairy herd and divided into 32 glass bottles which were assigned to eight different treatments (control, 3·0% CaO, 0·5% NaOH; 0·087%, 0·11% and 0·14% H2SO4; and 1·0 and 2·5% KMnO4). Treated dairy slurry samples were evaluated at 0, 1, 3, 7, 15, 30 and 60‐days following treatment application for viable MAP and dairy slurry pH, and in addition temperature in this material was monitored continuously. Bacterial counts were estimated at each sampling time. A Bayesian zero‐inflated Poisson mixed model was fitted to assess the effect of each treatment on the count of MAP cells. Model results indicated that only the 3·0% CaO treatment had a statistically important negative effect on MAP counts during the study period. For most treatments, MAP was undetectable immediately after chemical treatment but re‐appeared over time, in some replicates at low concentrations. However, in those cases MAP counts were not statistically different than the control treatment. Regarding the fate of the other bacterial populations, the Firmicutes phylum was the dominant population in the un‐treated slurry while Clostridia class members were among the most prevalent bacteria after the application of most chemical treatments.
Conclusion
Only 3% CaO treatment had a statistically important negative effect on MAP viability in cattle slurry.
Significance and Impact of the Study
This study provides evidence of MAP partial control in dairy slurry. This information should be considered as a best management practice to reduce MAP and other pathogens for slurry management on dairy farms.</abstract><cop>England</cop><pub>Oxford University Press</pub><pmid>31009147</pmid><doi>10.1111/jam.14288</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0002-2144-7982</orcidid></addata></record> |
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| ispartof | Journal of applied microbiology, 2019-08, Vol.127 (2), p.370-378 |
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| source | Wiley Online Library - AutoHoldings Journals; MEDLINE; Oxford University Press Journals All Titles (1996-Current) |
| subjects | Animals Anti-Bacterial Agents - pharmacology Bacteria Bayes Theorem Bayesian analysis Best management practices Calcium Compounds - pharmacology Cattle Cattle manure Chemical treatment Dairy cattle Dairy farms dairy slurry Dairying Farm management Farms Female Fertilizers Firmicutes Low concentrations Manure - microbiology Mycobacterium avium Mycobacterium avium subsp. paratuberculosis - drug effects Mycobacterium avium subsp. paratuberculosis - isolation & purification Mycobacterium avium subspecies paratuberculosis Organic chemistry Oxides - pharmacology Paratuberculosis Pathogens Populations Potassium permanganate Public health Slurries Sodium hydroxide Statistical methods Sulfuric acid Viability |
| title | Fate of Mycobacterium avium subsp. paratuberculosis and changes in bacterial diversity populations in dairy slurry after chemical treatments |
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