Prevalence and molecular epidemiology of Johne’s disease in Egyptian cattle
•Mycobacterium avium subsp. paractuberculosis circulating among cattle in Egypt.•Culture and real-time PCR have slight agreement for detection of MAP in feces.•Egyptian MAP strain is closely related to German and Netherland strains.•The importation of cattle must be from Johne’s free countries. John...
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| Veröffentlicht in: | Acta tropica 2019-07, Vol.195, p.1-5 |
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| creator | Selim, Abdelfattah Ali, Abdel-Fattah Ramadan, Eman |
| description | •Mycobacterium avium subsp. paractuberculosis circulating among cattle in Egypt.•Culture and real-time PCR have slight agreement for detection of MAP in feces.•Egyptian MAP strain is closely related to German and Netherland strains.•The importation of cattle must be from Johne’s free countries.
Johne’s disease is chronic enteric incurable disease in ruminant, is caused by Mycobacterium avium subsp. paratuberculosis (MAP). The data about incidence and economic importance of the disease in Egypt are scare. The present study investigated the prevalence of Johne`s disease among cattle in some localities in Egypt. The results revealed that the infection was significantly higher in older animals than young animals (P = |
| doi_str_mv | 10.1016/j.actatropica.2019.04.019 |
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Johne’s disease is chronic enteric incurable disease in ruminant, is caused by Mycobacterium avium subsp. paratuberculosis (MAP). The data about incidence and economic importance of the disease in Egypt are scare. The present study investigated the prevalence of Johne`s disease among cattle in some localities in Egypt. The results revealed that the infection was significantly higher in older animals than young animals (P = <0.05). The prevalence of the disease was higher in Gharbia governorate (19.6%) in comparison with other studied area. To determine the efficiency of diagnostic tests, 138 fecal samples were examined by culture and real-time PCR, the result showed slight agreement between both tests for detection of MAP in feces. Sequence analysis of IS1311 gene for isolated MAP strain from Egypt revealed high identity (98%) with other MAP strains from Germany and Netherland. MAP belongs to a subspecies genetically different from Mycobacterium avium subsp. Hominissuis (MAH) and Mycobacterium avium subsp. avium (MAA), therefore these results are as would be expected. This study confirms the circulation of MAP among cattle in Egypt and highlights the need for more study on the disease in the Middle East.</description><identifier>ISSN: 0001-706X</identifier><identifier>EISSN: 1873-6254</identifier><identifier>DOI: 10.1016/j.actatropica.2019.04.019</identifier><identifier>PMID: 31002806</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Culture ; ELISA ; Feces ; Paratuberculosis ; PCR ; Phylogenetic analysis</subject><ispartof>Acta tropica, 2019-07, Vol.195, p.1-5</ispartof><rights>2019 Elsevier B.V.</rights><rights>Copyright © 2019 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c377t-3f8fbe6ee6cc5070e37c0cfb458a308e9812860685bc2443682fb858b0a6f0b83</citedby><cites>FETCH-LOGICAL-c377t-3f8fbe6ee6cc5070e37c0cfb458a308e9812860685bc2443682fb858b0a6f0b83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0001706X18313779$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65534</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31002806$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Selim, Abdelfattah</creatorcontrib><creatorcontrib>Ali, Abdel-Fattah</creatorcontrib><creatorcontrib>Ramadan, Eman</creatorcontrib><title>Prevalence and molecular epidemiology of Johne’s disease in Egyptian cattle</title><title>Acta tropica</title><addtitle>Acta Trop</addtitle><description>•Mycobacterium avium subsp. paractuberculosis circulating among cattle in Egypt.•Culture and real-time PCR have slight agreement for detection of MAP in feces.•Egyptian MAP strain is closely related to German and Netherland strains.•The importation of cattle must be from Johne’s free countries.
Johne’s disease is chronic enteric incurable disease in ruminant, is caused by Mycobacterium avium subsp. paratuberculosis (MAP). The data about incidence and economic importance of the disease in Egypt are scare. The present study investigated the prevalence of Johne`s disease among cattle in some localities in Egypt. The results revealed that the infection was significantly higher in older animals than young animals (P = <0.05). The prevalence of the disease was higher in Gharbia governorate (19.6%) in comparison with other studied area. To determine the efficiency of diagnostic tests, 138 fecal samples were examined by culture and real-time PCR, the result showed slight agreement between both tests for detection of MAP in feces. Sequence analysis of IS1311 gene for isolated MAP strain from Egypt revealed high identity (98%) with other MAP strains from Germany and Netherland. MAP belongs to a subspecies genetically different from Mycobacterium avium subsp. Hominissuis (MAH) and Mycobacterium avium subsp. avium (MAA), therefore these results are as would be expected. This study confirms the circulation of MAP among cattle in Egypt and highlights the need for more study on the disease in the Middle East.</description><subject>Culture</subject><subject>ELISA</subject><subject>Feces</subject><subject>Paratuberculosis</subject><subject>PCR</subject><subject>Phylogenetic analysis</subject><issn>0001-706X</issn><issn>1873-6254</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNqNkM9KxDAQh4Mouq6-gsSbl9ZJ2qbZoyz-RdGDgreQplPN0jY1aRf25mv4ej6JXVbFo6cfA99vhvkIOWYQM2DidBFr0-veu84aHXNgsxjSeIwtMmEyTyLBs3SbTACARTmI5z2yH8JinHie8V2ylzAALkFMyN2Dx6WusTVIdVvSxtVohlp7ip0tsbGudi8r6ip6415b_Hz_CLS0AXVAalt6_rLqeqtbanTf13hAdipdBzz8zil5ujh_nF9Ft_eX1_Oz28gked5HSSWrAgWiMCaDHDDJDZiqSDOpE5A4k4xLAUJmheFpmgjJq0JmsgAtKihkMiUnm72dd28Dhl41Nhisa92iG4LinLFZKrMsHdHZBjXeheCxUp23jfYrxUCtbaqF-mNTrW0qSNUYY_fo-8xQNFj-Nn_0jcB8A-D47NKiV8HYtcvSejS9Kp39x5kvFDKNJw</recordid><startdate>20190701</startdate><enddate>20190701</enddate><creator>Selim, Abdelfattah</creator><creator>Ali, Abdel-Fattah</creator><creator>Ramadan, Eman</creator><general>Elsevier B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20190701</creationdate><title>Prevalence and molecular epidemiology of Johne’s disease in Egyptian cattle</title><author>Selim, Abdelfattah ; Ali, Abdel-Fattah ; Ramadan, Eman</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c377t-3f8fbe6ee6cc5070e37c0cfb458a308e9812860685bc2443682fb858b0a6f0b83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Culture</topic><topic>ELISA</topic><topic>Feces</topic><topic>Paratuberculosis</topic><topic>PCR</topic><topic>Phylogenetic analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Selim, Abdelfattah</creatorcontrib><creatorcontrib>Ali, Abdel-Fattah</creatorcontrib><creatorcontrib>Ramadan, Eman</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Acta tropica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Selim, Abdelfattah</au><au>Ali, Abdel-Fattah</au><au>Ramadan, Eman</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Prevalence and molecular epidemiology of Johne’s disease in Egyptian cattle</atitle><jtitle>Acta tropica</jtitle><addtitle>Acta Trop</addtitle><date>2019-07-01</date><risdate>2019</risdate><volume>195</volume><spage>1</spage><epage>5</epage><pages>1-5</pages><issn>0001-706X</issn><eissn>1873-6254</eissn><abstract>•Mycobacterium avium subsp. paractuberculosis circulating among cattle in Egypt.•Culture and real-time PCR have slight agreement for detection of MAP in feces.•Egyptian MAP strain is closely related to German and Netherland strains.•The importation of cattle must be from Johne’s free countries.
Johne’s disease is chronic enteric incurable disease in ruminant, is caused by Mycobacterium avium subsp. paratuberculosis (MAP). The data about incidence and economic importance of the disease in Egypt are scare. The present study investigated the prevalence of Johne`s disease among cattle in some localities in Egypt. The results revealed that the infection was significantly higher in older animals than young animals (P = <0.05). The prevalence of the disease was higher in Gharbia governorate (19.6%) in comparison with other studied area. To determine the efficiency of diagnostic tests, 138 fecal samples were examined by culture and real-time PCR, the result showed slight agreement between both tests for detection of MAP in feces. Sequence analysis of IS1311 gene for isolated MAP strain from Egypt revealed high identity (98%) with other MAP strains from Germany and Netherland. MAP belongs to a subspecies genetically different from Mycobacterium avium subsp. Hominissuis (MAH) and Mycobacterium avium subsp. avium (MAA), therefore these results are as would be expected. This study confirms the circulation of MAP among cattle in Egypt and highlights the need for more study on the disease in the Middle East.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>31002806</pmid><doi>10.1016/j.actatropica.2019.04.019</doi><tpages>5</tpages></addata></record> |
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| source | Elsevier ScienceDirect Journals Complete |
| subjects | Culture ELISA Feces Paratuberculosis PCR Phylogenetic analysis |
| title | Prevalence and molecular epidemiology of Johne’s disease in Egyptian cattle |
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