Studies on long term behavioural changes in group-housed rat models of brain and spinal cord injury using an automated home cage recording system
•Automated behaviour systems allow long-term assessment of complex CNS injury models.•Home cage grouped-housed animal are relevant for subtle spontaneous behaviour studies.•Spinal cord injury affects locomotion and the grooming /rearing ability of animals.•Group behaviour analysis facilitates the st...
Gespeichert in:
| Veröffentlicht in: | Journal of neuroscience methods 2019-06, Vol.321, p.49-63 |
|---|---|
| Hauptverfasser: | , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 63 |
|---|---|
| container_issue | |
| container_start_page | 49 |
| container_title | Journal of neuroscience methods |
| container_volume | 321 |
| creator | Yip, Ping K. Chapman, George E. Sillito, Rowland R. Ip, T.H. Richard Akhigbe, Georgia Becker, Stephanie C. Price, Anthony W. Michael-Titus, Adina T. Armstrong, J. Douglas Tremoleda, Jordi L. |
| description | •Automated behaviour systems allow long-term assessment of complex CNS injury models.•Home cage grouped-housed animal are relevant for subtle spontaneous behaviour studies.•Spinal cord injury affects locomotion and the grooming /rearing ability of animals.•Group behaviour analysis facilitates the study of aggression in brain injury models.•Home cage non-invasive behaviour systems provides a unique 3Rs advantage.
Neurotrauma patients face major neurological sequelae. The failure in the preclinical-to-clinical translation of candidate therapies could be due to poor evaluation of rodent behaviours after neurotrauma.
A home cage automated system was used to study the long term behaviour of individual rats with traumatic brain injury (TBI), spinal cord injury (SCI) and non-CNS injured controls, whilst group-housed in their home cages. Naïve rats were used as baseline controls. Automated locomotor activity and body temperature recordings were carried out 24 h /day for 3 days/week during 12 weeks post-injury. Behavioural patterns, including aggression, rearing, grooming, feeding and drinking were analysed from automated video recordings during week 1, 6 and 12.
SCI animals showed a lower locomotor activity compared to TBI or control animals during light and dark phases. TBI animals showed a higher aggression during the dark phase in the first week post-injury compared to SCI or control animals. Individual grooming and rearing were reduced in SCI animals compared to TBI and control animals in the first week post-injury during the dark phase. No differences in drinking or feeding were detected between groups. Locomotor activity did not differ between naïve male and female rats, but body temperature differ between light and dark phases for both.
Injury severity was compared to standard SCI and TBI behaviour scores (BBB and mNSS, respectively) and histological analysis.
This study demonstrates the practical benefits of using a non-intrusive automated home cage recording system to observe long term individual behaviour of group-housed SCI and TBI rats. |
| doi_str_mv | 10.1016/j.jneumeth.2019.04.005 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2210951967</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0165027019301165</els_id><sourcerecordid>2210951967</sourcerecordid><originalsourceid>FETCH-LOGICAL-c416t-ed4465570e717f53e69c9822c2de04947ec3f5f7f57a49868bb993ce73964c053</originalsourceid><addsrcrecordid>eNqFkc2O1DAQhC0EYoeFV1j5yCWhnR87voFWLCCtxAGQuFmO05k4SuLBPyvNY_DGOMwuV0596K-61FWE3DAoGTD-bi7nDdOKcSorYLKEpgRon5ED60RVcNH9fE4OGWwLqARckVchzADQSOAvyVUNUjKo4UB-f4tpsBio2-jitiON6Ffa46QfrEteL9RMejtmwG706F06FZNLAQfqdaSrG3DJ2pH2XmdAbwMNJ7vtMueHrJmTP9MUbL6s8z5Ft-qY1ZNbkRp9ROpxR3cgnEPE9TV5Meol4JvHeU1-3H38fvu5uP_66cvth_vCNIzHAoem4W0rAAUTY1sjl0Z2VWWqAfObjUBTj-2YV0I3suNd30tZGxS15I2Btr4mby93T979ShiiWm0wuCx6w_yhqioGsmWSi4zyC2q8C8HjqE7ertqfFQO116Fm9VSH2utQ0Cj463Hz6JH6FYd_sqf8M_D-AuQY8cGiV8FY3AwONucS1eDs_zz-AKcpobw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2210951967</pqid></control><display><type>article</type><title>Studies on long term behavioural changes in group-housed rat models of brain and spinal cord injury using an automated home cage recording system</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Yip, Ping K. ; Chapman, George E. ; Sillito, Rowland R. ; Ip, T.H. Richard ; Akhigbe, Georgia ; Becker, Stephanie C. ; Price, Anthony W. ; Michael-Titus, Adina T. ; Armstrong, J. Douglas ; Tremoleda, Jordi L.</creator><creatorcontrib>Yip, Ping K. ; Chapman, George E. ; Sillito, Rowland R. ; Ip, T.H. Richard ; Akhigbe, Georgia ; Becker, Stephanie C. ; Price, Anthony W. ; Michael-Titus, Adina T. ; Armstrong, J. Douglas ; Tremoleda, Jordi L.</creatorcontrib><description>•Automated behaviour systems allow long-term assessment of complex CNS injury models.•Home cage grouped-housed animal are relevant for subtle spontaneous behaviour studies.•Spinal cord injury affects locomotion and the grooming /rearing ability of animals.•Group behaviour analysis facilitates the study of aggression in brain injury models.•Home cage non-invasive behaviour systems provides a unique 3Rs advantage.
Neurotrauma patients face major neurological sequelae. The failure in the preclinical-to-clinical translation of candidate therapies could be due to poor evaluation of rodent behaviours after neurotrauma.
A home cage automated system was used to study the long term behaviour of individual rats with traumatic brain injury (TBI), spinal cord injury (SCI) and non-CNS injured controls, whilst group-housed in their home cages. Naïve rats were used as baseline controls. Automated locomotor activity and body temperature recordings were carried out 24 h /day for 3 days/week during 12 weeks post-injury. Behavioural patterns, including aggression, rearing, grooming, feeding and drinking were analysed from automated video recordings during week 1, 6 and 12.
SCI animals showed a lower locomotor activity compared to TBI or control animals during light and dark phases. TBI animals showed a higher aggression during the dark phase in the first week post-injury compared to SCI or control animals. Individual grooming and rearing were reduced in SCI animals compared to TBI and control animals in the first week post-injury during the dark phase. No differences in drinking or feeding were detected between groups. Locomotor activity did not differ between naïve male and female rats, but body temperature differ between light and dark phases for both.
Injury severity was compared to standard SCI and TBI behaviour scores (BBB and mNSS, respectively) and histological analysis.
This study demonstrates the practical benefits of using a non-intrusive automated home cage recording system to observe long term individual behaviour of group-housed SCI and TBI rats.</description><identifier>ISSN: 0165-0270</identifier><identifier>EISSN: 1872-678X</identifier><identifier>DOI: 10.1016/j.jneumeth.2019.04.005</identifier><identifier>PMID: 30991030</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>3Rs ; Animals ; Behavior, Animal ; Behaviour ; Body Temperature ; Brain Injuries, Traumatic - diagnosis ; Brain Injuries, Traumatic - psychology ; Disease Models, Animal ; Female ; Grooming ; Locomotion ; Male ; Neurotrauma ; Pattern Recognition, Automated ; Rats, Sprague-Dawley ; Spinal Cord Injuries - diagnosis ; Spinal Cord Injuries - psychology ; Spinal cord injury ; Traumatic brain injury ; Video Recording - methods</subject><ispartof>Journal of neuroscience methods, 2019-06, Vol.321, p.49-63</ispartof><rights>2019 Elsevier B.V.</rights><rights>Copyright © 2019 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c416t-ed4465570e717f53e69c9822c2de04947ec3f5f7f57a49868bb993ce73964c053</citedby><cites>FETCH-LOGICAL-c416t-ed4465570e717f53e69c9822c2de04947ec3f5f7f57a49868bb993ce73964c053</cites><orcidid>0000-0002-8846-9043</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0165027019301165$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30991030$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yip, Ping K.</creatorcontrib><creatorcontrib>Chapman, George E.</creatorcontrib><creatorcontrib>Sillito, Rowland R.</creatorcontrib><creatorcontrib>Ip, T.H. Richard</creatorcontrib><creatorcontrib>Akhigbe, Georgia</creatorcontrib><creatorcontrib>Becker, Stephanie C.</creatorcontrib><creatorcontrib>Price, Anthony W.</creatorcontrib><creatorcontrib>Michael-Titus, Adina T.</creatorcontrib><creatorcontrib>Armstrong, J. Douglas</creatorcontrib><creatorcontrib>Tremoleda, Jordi L.</creatorcontrib><title>Studies on long term behavioural changes in group-housed rat models of brain and spinal cord injury using an automated home cage recording system</title><title>Journal of neuroscience methods</title><addtitle>J Neurosci Methods</addtitle><description>•Automated behaviour systems allow long-term assessment of complex CNS injury models.•Home cage grouped-housed animal are relevant for subtle spontaneous behaviour studies.•Spinal cord injury affects locomotion and the grooming /rearing ability of animals.•Group behaviour analysis facilitates the study of aggression in brain injury models.•Home cage non-invasive behaviour systems provides a unique 3Rs advantage.
Neurotrauma patients face major neurological sequelae. The failure in the preclinical-to-clinical translation of candidate therapies could be due to poor evaluation of rodent behaviours after neurotrauma.
A home cage automated system was used to study the long term behaviour of individual rats with traumatic brain injury (TBI), spinal cord injury (SCI) and non-CNS injured controls, whilst group-housed in their home cages. Naïve rats were used as baseline controls. Automated locomotor activity and body temperature recordings were carried out 24 h /day for 3 days/week during 12 weeks post-injury. Behavioural patterns, including aggression, rearing, grooming, feeding and drinking were analysed from automated video recordings during week 1, 6 and 12.
SCI animals showed a lower locomotor activity compared to TBI or control animals during light and dark phases. TBI animals showed a higher aggression during the dark phase in the first week post-injury compared to SCI or control animals. Individual grooming and rearing were reduced in SCI animals compared to TBI and control animals in the first week post-injury during the dark phase. No differences in drinking or feeding were detected between groups. Locomotor activity did not differ between naïve male and female rats, but body temperature differ between light and dark phases for both.
Injury severity was compared to standard SCI and TBI behaviour scores (BBB and mNSS, respectively) and histological analysis.
This study demonstrates the practical benefits of using a non-intrusive automated home cage recording system to observe long term individual behaviour of group-housed SCI and TBI rats.</description><subject>3Rs</subject><subject>Animals</subject><subject>Behavior, Animal</subject><subject>Behaviour</subject><subject>Body Temperature</subject><subject>Brain Injuries, Traumatic - diagnosis</subject><subject>Brain Injuries, Traumatic - psychology</subject><subject>Disease Models, Animal</subject><subject>Female</subject><subject>Grooming</subject><subject>Locomotion</subject><subject>Male</subject><subject>Neurotrauma</subject><subject>Pattern Recognition, Automated</subject><subject>Rats, Sprague-Dawley</subject><subject>Spinal Cord Injuries - diagnosis</subject><subject>Spinal Cord Injuries - psychology</subject><subject>Spinal cord injury</subject><subject>Traumatic brain injury</subject><subject>Video Recording - methods</subject><issn>0165-0270</issn><issn>1872-678X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc2O1DAQhC0EYoeFV1j5yCWhnR87voFWLCCtxAGQuFmO05k4SuLBPyvNY_DGOMwuV0596K-61FWE3DAoGTD-bi7nDdOKcSorYLKEpgRon5ED60RVcNH9fE4OGWwLqARckVchzADQSOAvyVUNUjKo4UB-f4tpsBio2-jitiON6Ffa46QfrEteL9RMejtmwG706F06FZNLAQfqdaSrG3DJ2pH2XmdAbwMNJ7vtMueHrJmTP9MUbL6s8z5Ft-qY1ZNbkRp9ROpxR3cgnEPE9TV5Meol4JvHeU1-3H38fvu5uP_66cvth_vCNIzHAoem4W0rAAUTY1sjl0Z2VWWqAfObjUBTj-2YV0I3suNd30tZGxS15I2Btr4mby93T979ShiiWm0wuCx6w_yhqioGsmWSi4zyC2q8C8HjqE7ertqfFQO116Fm9VSH2utQ0Cj463Hz6JH6FYd_sqf8M_D-AuQY8cGiV8FY3AwONucS1eDs_zz-AKcpobw</recordid><startdate>20190601</startdate><enddate>20190601</enddate><creator>Yip, Ping K.</creator><creator>Chapman, George E.</creator><creator>Sillito, Rowland R.</creator><creator>Ip, T.H. Richard</creator><creator>Akhigbe, Georgia</creator><creator>Becker, Stephanie C.</creator><creator>Price, Anthony W.</creator><creator>Michael-Titus, Adina T.</creator><creator>Armstrong, J. Douglas</creator><creator>Tremoleda, Jordi L.</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-8846-9043</orcidid></search><sort><creationdate>20190601</creationdate><title>Studies on long term behavioural changes in group-housed rat models of brain and spinal cord injury using an automated home cage recording system</title><author>Yip, Ping K. ; Chapman, George E. ; Sillito, Rowland R. ; Ip, T.H. Richard ; Akhigbe, Georgia ; Becker, Stephanie C. ; Price, Anthony W. ; Michael-Titus, Adina T. ; Armstrong, J. Douglas ; Tremoleda, Jordi L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c416t-ed4465570e717f53e69c9822c2de04947ec3f5f7f57a49868bb993ce73964c053</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>3Rs</topic><topic>Animals</topic><topic>Behavior, Animal</topic><topic>Behaviour</topic><topic>Body Temperature</topic><topic>Brain Injuries, Traumatic - diagnosis</topic><topic>Brain Injuries, Traumatic - psychology</topic><topic>Disease Models, Animal</topic><topic>Female</topic><topic>Grooming</topic><topic>Locomotion</topic><topic>Male</topic><topic>Neurotrauma</topic><topic>Pattern Recognition, Automated</topic><topic>Rats, Sprague-Dawley</topic><topic>Spinal Cord Injuries - diagnosis</topic><topic>Spinal Cord Injuries - psychology</topic><topic>Spinal cord injury</topic><topic>Traumatic brain injury</topic><topic>Video Recording - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yip, Ping K.</creatorcontrib><creatorcontrib>Chapman, George E.</creatorcontrib><creatorcontrib>Sillito, Rowland R.</creatorcontrib><creatorcontrib>Ip, T.H. Richard</creatorcontrib><creatorcontrib>Akhigbe, Georgia</creatorcontrib><creatorcontrib>Becker, Stephanie C.</creatorcontrib><creatorcontrib>Price, Anthony W.</creatorcontrib><creatorcontrib>Michael-Titus, Adina T.</creatorcontrib><creatorcontrib>Armstrong, J. Douglas</creatorcontrib><creatorcontrib>Tremoleda, Jordi L.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of neuroscience methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yip, Ping K.</au><au>Chapman, George E.</au><au>Sillito, Rowland R.</au><au>Ip, T.H. Richard</au><au>Akhigbe, Georgia</au><au>Becker, Stephanie C.</au><au>Price, Anthony W.</au><au>Michael-Titus, Adina T.</au><au>Armstrong, J. Douglas</au><au>Tremoleda, Jordi L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Studies on long term behavioural changes in group-housed rat models of brain and spinal cord injury using an automated home cage recording system</atitle><jtitle>Journal of neuroscience methods</jtitle><addtitle>J Neurosci Methods</addtitle><date>2019-06-01</date><risdate>2019</risdate><volume>321</volume><spage>49</spage><epage>63</epage><pages>49-63</pages><issn>0165-0270</issn><eissn>1872-678X</eissn><abstract>•Automated behaviour systems allow long-term assessment of complex CNS injury models.•Home cage grouped-housed animal are relevant for subtle spontaneous behaviour studies.•Spinal cord injury affects locomotion and the grooming /rearing ability of animals.•Group behaviour analysis facilitates the study of aggression in brain injury models.•Home cage non-invasive behaviour systems provides a unique 3Rs advantage.
Neurotrauma patients face major neurological sequelae. The failure in the preclinical-to-clinical translation of candidate therapies could be due to poor evaluation of rodent behaviours after neurotrauma.
A home cage automated system was used to study the long term behaviour of individual rats with traumatic brain injury (TBI), spinal cord injury (SCI) and non-CNS injured controls, whilst group-housed in their home cages. Naïve rats were used as baseline controls. Automated locomotor activity and body temperature recordings were carried out 24 h /day for 3 days/week during 12 weeks post-injury. Behavioural patterns, including aggression, rearing, grooming, feeding and drinking were analysed from automated video recordings during week 1, 6 and 12.
SCI animals showed a lower locomotor activity compared to TBI or control animals during light and dark phases. TBI animals showed a higher aggression during the dark phase in the first week post-injury compared to SCI or control animals. Individual grooming and rearing were reduced in SCI animals compared to TBI and control animals in the first week post-injury during the dark phase. No differences in drinking or feeding were detected between groups. Locomotor activity did not differ between naïve male and female rats, but body temperature differ between light and dark phases for both.
Injury severity was compared to standard SCI and TBI behaviour scores (BBB and mNSS, respectively) and histological analysis.
This study demonstrates the practical benefits of using a non-intrusive automated home cage recording system to observe long term individual behaviour of group-housed SCI and TBI rats.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>30991030</pmid><doi>10.1016/j.jneumeth.2019.04.005</doi><tpages>15</tpages><orcidid>https://orcid.org/0000-0002-8846-9043</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0165-0270 |
| ispartof | Journal of neuroscience methods, 2019-06, Vol.321, p.49-63 |
| issn | 0165-0270 1872-678X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2210951967 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | 3Rs Animals Behavior, Animal Behaviour Body Temperature Brain Injuries, Traumatic - diagnosis Brain Injuries, Traumatic - psychology Disease Models, Animal Female Grooming Locomotion Male Neurotrauma Pattern Recognition, Automated Rats, Sprague-Dawley Spinal Cord Injuries - diagnosis Spinal Cord Injuries - psychology Spinal cord injury Traumatic brain injury Video Recording - methods |
| title | Studies on long term behavioural changes in group-housed rat models of brain and spinal cord injury using an automated home cage recording system |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-01T18%3A15%3A38IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Studies%20on%20long%20term%20behavioural%20changes%20in%20group-housed%20rat%20models%20of%20brain%20and%20spinal%20cord%20injury%20using%20an%20automated%20home%20cage%20recording%20system&rft.jtitle=Journal%20of%20neuroscience%20methods&rft.au=Yip,%20Ping%20K.&rft.date=2019-06-01&rft.volume=321&rft.spage=49&rft.epage=63&rft.pages=49-63&rft.issn=0165-0270&rft.eissn=1872-678X&rft_id=info:doi/10.1016/j.jneumeth.2019.04.005&rft_dat=%3Cproquest_cross%3E2210951967%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2210951967&rft_id=info:pmid/30991030&rft_els_id=S0165027019301165&rfr_iscdi=true |