Norovirus Detection at Oualidia Lagoon, a Moroccan Shellfish Harvesting Area, by Reverse Transcription PCR Analysis
Norovirus (NoV) is the leading cause of acute viral gastroenteritis outbreaks in the world. These outbreaks are frequently associated with bivalve shellfish consumption, particularly because these products are often eaten raw or only slightly cooked. In Morocco, regulations concerning the acceptable...
Gespeichert in:
| Veröffentlicht in: | Food and environmental virology 2019-09, Vol.11 (3), p.268-273 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 273 |
|---|---|
| container_issue | 3 |
| container_start_page | 268 |
| container_title | Food and environmental virology |
| container_volume | 11 |
| creator | El Moqri, N. El Mellouli, F. Hassou, N. Benhafid, M. Abouchoaib, N. Etahiri, S. |
| description | Norovirus (NoV) is the leading cause of acute viral gastroenteritis outbreaks in the world. These outbreaks are frequently associated with bivalve shellfish consumption, particularly because these products are often eaten raw or only slightly cooked. In Morocco, regulations concerning the acceptable levels of enteric bacteria indicator organisms in these products have been put in place. However, these regulations do not take into account the risk of viral contamination, and many gastroenteritis outbreaks have been linked to the ingestion of bivalve shellfish from areas that comply with the current food safety criteria. The aim of this study was to investigate NoV presence in shellfish samples (
n
= 104) collected at four sites owcff Oualidia lagoon (Moroccan Atlantic coast) from November 2015 to February 2017. Samples were analysed using real-time RT-PCR in accordance with the ISO 15216-2 method. NoVs of the genogroup II were detected in 7% of samples that were all collected during the winter months. Moreover, 71% of NoV-positive samples were harvested at sites upstream of the lagoon. These results highlight the need of regularly monitoring viral contamination in bivalve shellfish to limit the risk of viral gastroenteritis outbreaks. |
| doi_str_mv | 10.1007/s12560-019-09386-0 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2210008502</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2210008502</sourcerecordid><originalsourceid>FETCH-LOGICAL-c375t-a63aec989b6e6fa808e4cf91d1627a5850f2bd63f29cf10359590a9e8e226eca3</originalsourceid><addsrcrecordid>eNp9kU1PGzEQhi0E4qv9AxyQpV44sO3YznrXxyhAqRRKRenZmjizwWizDvZupPx7DKFU6oGTLfmZ16_mYexEwFcBUH1LQpYaChCmAKNqXcAOOxS1rgpQI7n7flejA3aU0iOAVrJU--xAgamlEPKQpZ8hhrWPQ-IX1JPrfeg49vx2wNbPPfIpLkLozjnym0w6hx3__UBt2_j0wK8xrin1vlvwcSQ857MNv6M1xUT8PmKXXPSr18hfkzs-7rDdJJ8-sb0G20Sf385j9ufq8n5yXUxvv_-YjKeFU1XZF6gVkjO1mWnSDdZQ08g1RsyFlhWWdQmNnM21aqRxjQBVmtIAGqpJSk0O1TE72-auYngack-79Mnl7thRGJKVMq8Rco7M6Jf_0McwxNz3haqg1EaMTKbklnIxpBSpsavolxg3VoB9UWK3SmxWYl-VWMhDp2_Rw2xJ8_eRvw4yoLZAyk_dguK_vz-IfQZ4QpaK</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2270569149</pqid></control><display><type>article</type><title>Norovirus Detection at Oualidia Lagoon, a Moroccan Shellfish Harvesting Area, by Reverse Transcription PCR Analysis</title><source>MEDLINE</source><source>SpringerLink Journals - AutoHoldings</source><creator>El Moqri, N. ; El Mellouli, F. ; Hassou, N. ; Benhafid, M. ; Abouchoaib, N. ; Etahiri, S.</creator><creatorcontrib>El Moqri, N. ; El Mellouli, F. ; Hassou, N. ; Benhafid, M. ; Abouchoaib, N. ; Etahiri, S.</creatorcontrib><description>Norovirus (NoV) is the leading cause of acute viral gastroenteritis outbreaks in the world. These outbreaks are frequently associated with bivalve shellfish consumption, particularly because these products are often eaten raw or only slightly cooked. In Morocco, regulations concerning the acceptable levels of enteric bacteria indicator organisms in these products have been put in place. However, these regulations do not take into account the risk of viral contamination, and many gastroenteritis outbreaks have been linked to the ingestion of bivalve shellfish from areas that comply with the current food safety criteria. The aim of this study was to investigate NoV presence in shellfish samples (
n
= 104) collected at four sites owcff Oualidia lagoon (Moroccan Atlantic coast) from November 2015 to February 2017. Samples were analysed using real-time RT-PCR in accordance with the ISO 15216-2 method. NoVs of the genogroup II were detected in 7% of samples that were all collected during the winter months. Moreover, 71% of NoV-positive samples were harvested at sites upstream of the lagoon. These results highlight the need of regularly monitoring viral contamination in bivalve shellfish to limit the risk of viral gastroenteritis outbreaks.</description><identifier>ISSN: 1867-0334</identifier><identifier>EISSN: 1867-0342</identifier><identifier>DOI: 10.1007/s12560-019-09386-0</identifier><identifier>PMID: 30982112</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Animals ; Biomedical and Life Sciences ; Biomedicine ; Bivalvia ; Bivalvia - growth & development ; Bivalvia - virology ; Chemistry/Food Science ; Contamination ; Environmental monitoring ; Food contamination ; Food Contamination - analysis ; Food safety ; Food Science ; Gastroenteritis ; Gastroenteritis - virology ; Harvesting ; Humans ; Ingestion ; Lagoons ; Morocco ; Norovirus ; Norovirus - classification ; Norovirus - genetics ; Norovirus - isolation & purification ; Original Paper ; Outbreaks ; Polymerase chain reaction ; Real-Time Polymerase Chain Reaction ; Regulations ; Reverse transcription ; Seasons ; Seawater - virology ; Shellfish ; Shellfish - virology ; Virology</subject><ispartof>Food and environmental virology, 2019-09, Vol.11 (3), p.268-273</ispartof><rights>Springer Science+Business Media, LLC, part of Springer Nature 2019</rights><rights>Copyright Springer Nature B.V. 2019</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c375t-a63aec989b6e6fa808e4cf91d1627a5850f2bd63f29cf10359590a9e8e226eca3</citedby><cites>FETCH-LOGICAL-c375t-a63aec989b6e6fa808e4cf91d1627a5850f2bd63f29cf10359590a9e8e226eca3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s12560-019-09386-0$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s12560-019-09386-0$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30982112$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>El Moqri, N.</creatorcontrib><creatorcontrib>El Mellouli, F.</creatorcontrib><creatorcontrib>Hassou, N.</creatorcontrib><creatorcontrib>Benhafid, M.</creatorcontrib><creatorcontrib>Abouchoaib, N.</creatorcontrib><creatorcontrib>Etahiri, S.</creatorcontrib><title>Norovirus Detection at Oualidia Lagoon, a Moroccan Shellfish Harvesting Area, by Reverse Transcription PCR Analysis</title><title>Food and environmental virology</title><addtitle>Food Environ Virol</addtitle><addtitle>Food Environ Virol</addtitle><description>Norovirus (NoV) is the leading cause of acute viral gastroenteritis outbreaks in the world. These outbreaks are frequently associated with bivalve shellfish consumption, particularly because these products are often eaten raw or only slightly cooked. In Morocco, regulations concerning the acceptable levels of enteric bacteria indicator organisms in these products have been put in place. However, these regulations do not take into account the risk of viral contamination, and many gastroenteritis outbreaks have been linked to the ingestion of bivalve shellfish from areas that comply with the current food safety criteria. The aim of this study was to investigate NoV presence in shellfish samples (
n
= 104) collected at four sites owcff Oualidia lagoon (Moroccan Atlantic coast) from November 2015 to February 2017. Samples were analysed using real-time RT-PCR in accordance with the ISO 15216-2 method. NoVs of the genogroup II were detected in 7% of samples that were all collected during the winter months. Moreover, 71% of NoV-positive samples were harvested at sites upstream of the lagoon. These results highlight the need of regularly monitoring viral contamination in bivalve shellfish to limit the risk of viral gastroenteritis outbreaks.</description><subject>Animals</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Bivalvia</subject><subject>Bivalvia - growth & development</subject><subject>Bivalvia - virology</subject><subject>Chemistry/Food Science</subject><subject>Contamination</subject><subject>Environmental monitoring</subject><subject>Food contamination</subject><subject>Food Contamination - analysis</subject><subject>Food safety</subject><subject>Food Science</subject><subject>Gastroenteritis</subject><subject>Gastroenteritis - virology</subject><subject>Harvesting</subject><subject>Humans</subject><subject>Ingestion</subject><subject>Lagoons</subject><subject>Morocco</subject><subject>Norovirus</subject><subject>Norovirus - classification</subject><subject>Norovirus - genetics</subject><subject>Norovirus - isolation & purification</subject><subject>Original Paper</subject><subject>Outbreaks</subject><subject>Polymerase chain reaction</subject><subject>Real-Time Polymerase Chain Reaction</subject><subject>Regulations</subject><subject>Reverse transcription</subject><subject>Seasons</subject><subject>Seawater - virology</subject><subject>Shellfish</subject><subject>Shellfish - virology</subject><subject>Virology</subject><issn>1867-0334</issn><issn>1867-0342</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kU1PGzEQhi0E4qv9AxyQpV44sO3YznrXxyhAqRRKRenZmjizwWizDvZupPx7DKFU6oGTLfmZ16_mYexEwFcBUH1LQpYaChCmAKNqXcAOOxS1rgpQI7n7flejA3aU0iOAVrJU--xAgamlEPKQpZ8hhrWPQ-IX1JPrfeg49vx2wNbPPfIpLkLozjnym0w6hx3__UBt2_j0wK8xrin1vlvwcSQ857MNv6M1xUT8PmKXXPSr18hfkzs-7rDdJJ8-sb0G20Sf385j9ufq8n5yXUxvv_-YjKeFU1XZF6gVkjO1mWnSDdZQ08g1RsyFlhWWdQmNnM21aqRxjQBVmtIAGqpJSk0O1TE72-auYngack-79Mnl7thRGJKVMq8Rco7M6Jf_0McwxNz3haqg1EaMTKbklnIxpBSpsavolxg3VoB9UWK3SmxWYl-VWMhDp2_Rw2xJ8_eRvw4yoLZAyk_dguK_vz-IfQZ4QpaK</recordid><startdate>20190901</startdate><enddate>20190901</enddate><creator>El Moqri, N.</creator><creator>El Mellouli, F.</creator><creator>Hassou, N.</creator><creator>Benhafid, M.</creator><creator>Abouchoaib, N.</creator><creator>Etahiri, S.</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20190901</creationdate><title>Norovirus Detection at Oualidia Lagoon, a Moroccan Shellfish Harvesting Area, by Reverse Transcription PCR Analysis</title><author>El Moqri, N. ; El Mellouli, F. ; Hassou, N. ; Benhafid, M. ; Abouchoaib, N. ; Etahiri, S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c375t-a63aec989b6e6fa808e4cf91d1627a5850f2bd63f29cf10359590a9e8e226eca3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Animals</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Bivalvia</topic><topic>Bivalvia - growth & development</topic><topic>Bivalvia - virology</topic><topic>Chemistry/Food Science</topic><topic>Contamination</topic><topic>Environmental monitoring</topic><topic>Food contamination</topic><topic>Food Contamination - analysis</topic><topic>Food safety</topic><topic>Food Science</topic><topic>Gastroenteritis</topic><topic>Gastroenteritis - virology</topic><topic>Harvesting</topic><topic>Humans</topic><topic>Ingestion</topic><topic>Lagoons</topic><topic>Morocco</topic><topic>Norovirus</topic><topic>Norovirus - classification</topic><topic>Norovirus - genetics</topic><topic>Norovirus - isolation & purification</topic><topic>Original Paper</topic><topic>Outbreaks</topic><topic>Polymerase chain reaction</topic><topic>Real-Time Polymerase Chain Reaction</topic><topic>Regulations</topic><topic>Reverse transcription</topic><topic>Seasons</topic><topic>Seawater - virology</topic><topic>Shellfish</topic><topic>Shellfish - virology</topic><topic>Virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>El Moqri, N.</creatorcontrib><creatorcontrib>El Mellouli, F.</creatorcontrib><creatorcontrib>Hassou, N.</creatorcontrib><creatorcontrib>Benhafid, M.</creatorcontrib><creatorcontrib>Abouchoaib, N.</creatorcontrib><creatorcontrib>Etahiri, S.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Food and environmental virology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>El Moqri, N.</au><au>El Mellouli, F.</au><au>Hassou, N.</au><au>Benhafid, M.</au><au>Abouchoaib, N.</au><au>Etahiri, S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Norovirus Detection at Oualidia Lagoon, a Moroccan Shellfish Harvesting Area, by Reverse Transcription PCR Analysis</atitle><jtitle>Food and environmental virology</jtitle><stitle>Food Environ Virol</stitle><addtitle>Food Environ Virol</addtitle><date>2019-09-01</date><risdate>2019</risdate><volume>11</volume><issue>3</issue><spage>268</spage><epage>273</epage><pages>268-273</pages><issn>1867-0334</issn><eissn>1867-0342</eissn><abstract>Norovirus (NoV) is the leading cause of acute viral gastroenteritis outbreaks in the world. These outbreaks are frequently associated with bivalve shellfish consumption, particularly because these products are often eaten raw or only slightly cooked. In Morocco, regulations concerning the acceptable levels of enteric bacteria indicator organisms in these products have been put in place. However, these regulations do not take into account the risk of viral contamination, and many gastroenteritis outbreaks have been linked to the ingestion of bivalve shellfish from areas that comply with the current food safety criteria. The aim of this study was to investigate NoV presence in shellfish samples (
n
= 104) collected at four sites owcff Oualidia lagoon (Moroccan Atlantic coast) from November 2015 to February 2017. Samples were analysed using real-time RT-PCR in accordance with the ISO 15216-2 method. NoVs of the genogroup II were detected in 7% of samples that were all collected during the winter months. Moreover, 71% of NoV-positive samples were harvested at sites upstream of the lagoon. These results highlight the need of regularly monitoring viral contamination in bivalve shellfish to limit the risk of viral gastroenteritis outbreaks.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>30982112</pmid><doi>10.1007/s12560-019-09386-0</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1867-0334 |
| ispartof | Food and environmental virology, 2019-09, Vol.11 (3), p.268-273 |
| issn | 1867-0334 1867-0342 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2210008502 |
| source | MEDLINE; SpringerLink Journals - AutoHoldings |
| subjects | Animals Biomedical and Life Sciences Biomedicine Bivalvia Bivalvia - growth & development Bivalvia - virology Chemistry/Food Science Contamination Environmental monitoring Food contamination Food Contamination - analysis Food safety Food Science Gastroenteritis Gastroenteritis - virology Harvesting Humans Ingestion Lagoons Morocco Norovirus Norovirus - classification Norovirus - genetics Norovirus - isolation & purification Original Paper Outbreaks Polymerase chain reaction Real-Time Polymerase Chain Reaction Regulations Reverse transcription Seasons Seawater - virology Shellfish Shellfish - virology Virology |
| title | Norovirus Detection at Oualidia Lagoon, a Moroccan Shellfish Harvesting Area, by Reverse Transcription PCR Analysis |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-07T17%3A17%3A38IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Norovirus%20Detection%20at%20Oualidia%20Lagoon,%20a%20Moroccan%20Shellfish%20Harvesting%20Area,%20by%20Reverse%20Transcription%20PCR%20Analysis&rft.jtitle=Food%20and%20environmental%20virology&rft.au=El%20Moqri,%20N.&rft.date=2019-09-01&rft.volume=11&rft.issue=3&rft.spage=268&rft.epage=273&rft.pages=268-273&rft.issn=1867-0334&rft.eissn=1867-0342&rft_id=info:doi/10.1007/s12560-019-09386-0&rft_dat=%3Cproquest_cross%3E2210008502%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2270569149&rft_id=info:pmid/30982112&rfr_iscdi=true |