Minichromosomal Maintenance Component Complex 5 (MCM5) as a Marker of Barrett’s Esophagus-Related Neoplasia: A Feasibility Study
Background The endoscopic detection of esophageal cancer is suboptimal in both patients referred with dyspeptic symptoms and those enrolled in Barrett’s surveillance programs. MCM5 expression in cells collected from gastric fluid may be correlated with the presence of dysplasia or adenocarcinoma. An...
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| Veröffentlicht in: | Digestive diseases and sciences 2019-10, Vol.64 (10), p.2815-2822 |
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| creator | Everson, M. Magee, C. Alzoubaidi, D. Brogden, S. Graham, D. Lovat, L. B. Novelli, M. Haidry, R. |
| description | Background
The endoscopic detection of esophageal cancer is suboptimal in both patients referred with dyspeptic symptoms and those enrolled in Barrett’s surveillance programs. MCM5 expression in cells collected from gastric fluid may be correlated with the presence of dysplasia or adenocarcinoma. Analysis of this biomarker may improve the detection of cancer.
Methods
Sixty-one patients were enrolled at a single UK referral center. From each patient, 5–10 ml of gastric fluid was aspirated endoscopically. Patients were categorized according to their histology, normal, non-dysplastic Barrett’s (NDBE), high-grade dysplastic Barrett’s (HGD), and esophageal adenocarcinoma (EAC). All histology was confirmed by Seattle protocol biopsies or endoscopic mucosal resection. Samples were centrifuged, and the cell pellet was lysed. MCM5 expression levels were quantified using a proprietary immunoassay. The mean MCM5 expression was compared between groups by Kruskal–Wallis test. ROC curves were also used to assess diagnostic utility.
Results
The mean expression of MCM5 increases as patients progress from a normal esophagus to NDBE, HGD, and EAC (14.4; 49.8; 112.3; and 154.1, respectively). There was a significant difference in the MCM5 expression of patients with a normal esophagus compared to those with EAC (
p
= 0.04). There was a trend toward higher MCM5 expression in patients with EAC compared to those with NDBE (
p
= 0.34). MCM5 expression was a fair discriminator (AUC 0.70 [95% CI 0.57–0.83]) between patients without neoplasia (normal and NDBE) and those with early neoplasia (HGD and EAC).
Conclusion
MCM5 expression in gastric fluid samples can differentiate patients with a histologically normal esophagus compared to those with early adenocarcinoma. Larger, powered studies are needed to assess whether it can be used to differentiate those with HGD from NDBE. |
| doi_str_mv | 10.1007/s10620-019-05607-5 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_proqu</sourceid><recordid>TN_cdi_proquest_miscellaneous_2210007253</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A712936703</galeid><sourcerecordid>A712936703</sourcerecordid><originalsourceid>FETCH-LOGICAL-c486t-b69e4f1558af2724b77d2ecb764d36ee43ff800b3e72884d6987751ab5fde1413</originalsourceid><addsrcrecordid>eNp9kc1u1DAUhS0EokPhBVggS2zKIsV2YjthN4xaQOqAxM_acpLrqUtip7YjMbuqb8Hr8SR4OoUKhJAXvnK-c3RzDkJPKTmmhMiXkRLBSEFoUxAuiCz4PbSgXJYF46K-jxaEijxTKg7QoxgvCCGNpOIhOihJUzNGyQJdr62z3Xnwo49-1ANea-sSOO06wCs_Tt6BSzfTAN8wx0fr1Zq_wDpindnwFQL2Br_WIUBKP66-R3wS_XSuN3MsPsKgE_T4Pfhp0NHqV3iJTyFPrR1s2uJPae63j9EDo4cIT27vQ_Tl9OTz6m1x9uHNu9XyrOiqWqSiFQ1UhnJea8Mkq1opewZdK0XVlwKgKo2pCWlLkKyuq140tZSc6pabHmhFy0N0tPedgr-cISY12tjBMGgHfo5qF0iOlfEyo8__Qi_8HFzeLlOkoTnyWtxRGz2Ass74FHS3M1VLSVlTCkl2Xsf_oPLpYbRdjtfY_P6HgO0FXfAxBjBqCnbUYasoUbvi1b54lbdQN8UrnkXPbjee2xH635JfTWeg3AMxf3IbCHe_9B_bn_uStts</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2209101986</pqid></control><display><type>article</type><title>Minichromosomal Maintenance Component Complex 5 (MCM5) as a Marker of Barrett’s Esophagus-Related Neoplasia: A Feasibility Study</title><source>MEDLINE</source><source>SpringerLink Journals</source><creator>Everson, M. ; Magee, C. ; Alzoubaidi, D. ; Brogden, S. ; Graham, D. ; Lovat, L. B. ; Novelli, M. ; Haidry, R.</creator><creatorcontrib>Everson, M. ; Magee, C. ; Alzoubaidi, D. ; Brogden, S. ; Graham, D. ; Lovat, L. B. ; Novelli, M. ; Haidry, R.</creatorcontrib><description>Background
The endoscopic detection of esophageal cancer is suboptimal in both patients referred with dyspeptic symptoms and those enrolled in Barrett’s surveillance programs. MCM5 expression in cells collected from gastric fluid may be correlated with the presence of dysplasia or adenocarcinoma. Analysis of this biomarker may improve the detection of cancer.
Methods
Sixty-one patients were enrolled at a single UK referral center. From each patient, 5–10 ml of gastric fluid was aspirated endoscopically. Patients were categorized according to their histology, normal, non-dysplastic Barrett’s (NDBE), high-grade dysplastic Barrett’s (HGD), and esophageal adenocarcinoma (EAC). All histology was confirmed by Seattle protocol biopsies or endoscopic mucosal resection. Samples were centrifuged, and the cell pellet was lysed. MCM5 expression levels were quantified using a proprietary immunoassay. The mean MCM5 expression was compared between groups by Kruskal–Wallis test. ROC curves were also used to assess diagnostic utility.
Results
The mean expression of MCM5 increases as patients progress from a normal esophagus to NDBE, HGD, and EAC (14.4; 49.8; 112.3; and 154.1, respectively). There was a significant difference in the MCM5 expression of patients with a normal esophagus compared to those with EAC (
p
= 0.04). There was a trend toward higher MCM5 expression in patients with EAC compared to those with NDBE (
p
= 0.34). MCM5 expression was a fair discriminator (AUC 0.70 [95% CI 0.57–0.83]) between patients without neoplasia (normal and NDBE) and those with early neoplasia (HGD and EAC).
Conclusion
MCM5 expression in gastric fluid samples can differentiate patients with a histologically normal esophagus compared to those with early adenocarcinoma. Larger, powered studies are needed to assess whether it can be used to differentiate those with HGD from NDBE.</description><identifier>ISSN: 0163-2116</identifier><identifier>EISSN: 1573-2568</identifier><identifier>DOI: 10.1007/s10620-019-05607-5</identifier><identifier>PMID: 30982210</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Adenocarcinoma - diagnosis ; Adenocarcinoma - metabolism ; Adenocarcinoma - pathology ; Barrett Esophagus - diagnosis ; Barrett Esophagus - metabolism ; Barrett Esophagus - pathology ; Biochemistry ; Biomarkers ; Biomarkers - analysis ; Biopsy - methods ; Cancer ; Cell Cycle Proteins - analysis ; Cost analysis ; Deoxyribonucleic acid ; Diagnosis ; Disease Progression ; DNA ; DNA Replication ; Dysplasia ; Early Detection of Cancer - methods ; Endoscopy ; Endoscopy, Digestive System - methods ; Esophageal cancer ; Esophageal Neoplasms - diagnosis ; Esophageal Neoplasms - metabolism ; Esophageal Neoplasms - pathology ; Feasibility Studies ; Female ; Gastric Juice - metabolism ; Gastroenterology ; Hepatology ; Histology ; Humans ; Immunoassay ; Male ; Medicine ; Medicine & Public Health ; Middle Aged ; Oncology ; Original Article ; Pathology ; Patients ; Precancerous Conditions - diagnosis ; Precancerous Conditions - metabolism ; Precancerous Conditions - pathology ; Proteins ; Surveillance ; Systematic review ; Transplant Surgery ; University colleges ; Urine</subject><ispartof>Digestive diseases and sciences, 2019-10, Vol.64 (10), p.2815-2822</ispartof><rights>Springer Science+Business Media, LLC, part of Springer Nature 2019</rights><rights>COPYRIGHT 2019 Springer</rights><rights>Digestive Diseases and Sciences is a copyright of Springer, (2019). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c486t-b69e4f1558af2724b77d2ecb764d36ee43ff800b3e72884d6987751ab5fde1413</citedby><cites>FETCH-LOGICAL-c486t-b69e4f1558af2724b77d2ecb764d36ee43ff800b3e72884d6987751ab5fde1413</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10620-019-05607-5$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10620-019-05607-5$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30982210$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Everson, M.</creatorcontrib><creatorcontrib>Magee, C.</creatorcontrib><creatorcontrib>Alzoubaidi, D.</creatorcontrib><creatorcontrib>Brogden, S.</creatorcontrib><creatorcontrib>Graham, D.</creatorcontrib><creatorcontrib>Lovat, L. B.</creatorcontrib><creatorcontrib>Novelli, M.</creatorcontrib><creatorcontrib>Haidry, R.</creatorcontrib><title>Minichromosomal Maintenance Component Complex 5 (MCM5) as a Marker of Barrett’s Esophagus-Related Neoplasia: A Feasibility Study</title><title>Digestive diseases and sciences</title><addtitle>Dig Dis Sci</addtitle><addtitle>Dig Dis Sci</addtitle><description>Background
The endoscopic detection of esophageal cancer is suboptimal in both patients referred with dyspeptic symptoms and those enrolled in Barrett’s surveillance programs. MCM5 expression in cells collected from gastric fluid may be correlated with the presence of dysplasia or adenocarcinoma. Analysis of this biomarker may improve the detection of cancer.
Methods
Sixty-one patients were enrolled at a single UK referral center. From each patient, 5–10 ml of gastric fluid was aspirated endoscopically. Patients were categorized according to their histology, normal, non-dysplastic Barrett’s (NDBE), high-grade dysplastic Barrett’s (HGD), and esophageal adenocarcinoma (EAC). All histology was confirmed by Seattle protocol biopsies or endoscopic mucosal resection. Samples were centrifuged, and the cell pellet was lysed. MCM5 expression levels were quantified using a proprietary immunoassay. The mean MCM5 expression was compared between groups by Kruskal–Wallis test. ROC curves were also used to assess diagnostic utility.
Results
The mean expression of MCM5 increases as patients progress from a normal esophagus to NDBE, HGD, and EAC (14.4; 49.8; 112.3; and 154.1, respectively). There was a significant difference in the MCM5 expression of patients with a normal esophagus compared to those with EAC (
p
= 0.04). There was a trend toward higher MCM5 expression in patients with EAC compared to those with NDBE (
p
= 0.34). MCM5 expression was a fair discriminator (AUC 0.70 [95% CI 0.57–0.83]) between patients without neoplasia (normal and NDBE) and those with early neoplasia (HGD and EAC).
Conclusion
MCM5 expression in gastric fluid samples can differentiate patients with a histologically normal esophagus compared to those with early adenocarcinoma. Larger, powered studies are needed to assess whether it can be used to differentiate those with HGD from NDBE.</description><subject>Adenocarcinoma - diagnosis</subject><subject>Adenocarcinoma - metabolism</subject><subject>Adenocarcinoma - pathology</subject><subject>Barrett Esophagus - diagnosis</subject><subject>Barrett Esophagus - metabolism</subject><subject>Barrett Esophagus - pathology</subject><subject>Biochemistry</subject><subject>Biomarkers</subject><subject>Biomarkers - analysis</subject><subject>Biopsy - methods</subject><subject>Cancer</subject><subject>Cell Cycle Proteins - analysis</subject><subject>Cost analysis</subject><subject>Deoxyribonucleic acid</subject><subject>Diagnosis</subject><subject>Disease Progression</subject><subject>DNA</subject><subject>DNA Replication</subject><subject>Dysplasia</subject><subject>Early Detection of Cancer - methods</subject><subject>Endoscopy</subject><subject>Endoscopy, Digestive System - methods</subject><subject>Esophageal cancer</subject><subject>Esophageal Neoplasms - diagnosis</subject><subject>Esophageal Neoplasms - metabolism</subject><subject>Esophageal Neoplasms - pathology</subject><subject>Feasibility Studies</subject><subject>Female</subject><subject>Gastric Juice - metabolism</subject><subject>Gastroenterology</subject><subject>Hepatology</subject><subject>Histology</subject><subject>Humans</subject><subject>Immunoassay</subject><subject>Male</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Middle Aged</subject><subject>Oncology</subject><subject>Original Article</subject><subject>Pathology</subject><subject>Patients</subject><subject>Precancerous Conditions - diagnosis</subject><subject>Precancerous Conditions - metabolism</subject><subject>Precancerous Conditions - pathology</subject><subject>Proteins</subject><subject>Surveillance</subject><subject>Systematic review</subject><subject>Transplant Surgery</subject><subject>University colleges</subject><subject>Urine</subject><issn>0163-2116</issn><issn>1573-2568</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp9kc1u1DAUhS0EokPhBVggS2zKIsV2YjthN4xaQOqAxM_acpLrqUtip7YjMbuqb8Hr8SR4OoUKhJAXvnK-c3RzDkJPKTmmhMiXkRLBSEFoUxAuiCz4PbSgXJYF46K-jxaEijxTKg7QoxgvCCGNpOIhOihJUzNGyQJdr62z3Xnwo49-1ANea-sSOO06wCs_Tt6BSzfTAN8wx0fr1Zq_wDpindnwFQL2Br_WIUBKP66-R3wS_XSuN3MsPsKgE_T4Pfhp0NHqV3iJTyFPrR1s2uJPae63j9EDo4cIT27vQ_Tl9OTz6m1x9uHNu9XyrOiqWqSiFQ1UhnJea8Mkq1opewZdK0XVlwKgKo2pCWlLkKyuq140tZSc6pabHmhFy0N0tPedgr-cISY12tjBMGgHfo5qF0iOlfEyo8__Qi_8HFzeLlOkoTnyWtxRGz2Ass74FHS3M1VLSVlTCkl2Xsf_oPLpYbRdjtfY_P6HgO0FXfAxBjBqCnbUYasoUbvi1b54lbdQN8UrnkXPbjee2xH635JfTWeg3AMxf3IbCHe_9B_bn_uStts</recordid><startdate>20191001</startdate><enddate>20191001</enddate><creator>Everson, M.</creator><creator>Magee, C.</creator><creator>Alzoubaidi, D.</creator><creator>Brogden, S.</creator><creator>Graham, D.</creator><creator>Lovat, L. B.</creator><creator>Novelli, M.</creator><creator>Haidry, R.</creator><general>Springer US</general><general>Springer</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9-</scope><scope>K9.</scope><scope>KB0</scope><scope>M0R</scope><scope>M0S</scope><scope>M1P</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>20191001</creationdate><title>Minichromosomal Maintenance Component Complex 5 (MCM5) as a Marker of Barrett’s Esophagus-Related Neoplasia: A Feasibility Study</title><author>Everson, M. ; Magee, C. ; Alzoubaidi, D. ; Brogden, S. ; Graham, D. ; Lovat, L. B. ; Novelli, M. ; Haidry, R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c486t-b69e4f1558af2724b77d2ecb764d36ee43ff800b3e72884d6987751ab5fde1413</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Adenocarcinoma - diagnosis</topic><topic>Adenocarcinoma - metabolism</topic><topic>Adenocarcinoma - pathology</topic><topic>Barrett Esophagus - diagnosis</topic><topic>Barrett Esophagus - metabolism</topic><topic>Barrett Esophagus - pathology</topic><topic>Biochemistry</topic><topic>Biomarkers</topic><topic>Biomarkers - analysis</topic><topic>Biopsy - methods</topic><topic>Cancer</topic><topic>Cell Cycle Proteins - analysis</topic><topic>Cost analysis</topic><topic>Deoxyribonucleic acid</topic><topic>Diagnosis</topic><topic>Disease Progression</topic><topic>DNA</topic><topic>DNA Replication</topic><topic>Dysplasia</topic><topic>Early Detection of Cancer - methods</topic><topic>Endoscopy</topic><topic>Endoscopy, Digestive System - methods</topic><topic>Esophageal cancer</topic><topic>Esophageal Neoplasms - diagnosis</topic><topic>Esophageal Neoplasms - metabolism</topic><topic>Esophageal Neoplasms - pathology</topic><topic>Feasibility Studies</topic><topic>Female</topic><topic>Gastric Juice - metabolism</topic><topic>Gastroenterology</topic><topic>Hepatology</topic><topic>Histology</topic><topic>Humans</topic><topic>Immunoassay</topic><topic>Male</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Middle Aged</topic><topic>Oncology</topic><topic>Original Article</topic><topic>Pathology</topic><topic>Patients</topic><topic>Precancerous Conditions - diagnosis</topic><topic>Precancerous Conditions - metabolism</topic><topic>Precancerous Conditions - pathology</topic><topic>Proteins</topic><topic>Surveillance</topic><topic>Systematic review</topic><topic>Transplant Surgery</topic><topic>University colleges</topic><topic>Urine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Everson, M.</creatorcontrib><creatorcontrib>Magee, C.</creatorcontrib><creatorcontrib>Alzoubaidi, D.</creatorcontrib><creatorcontrib>Brogden, S.</creatorcontrib><creatorcontrib>Graham, D.</creatorcontrib><creatorcontrib>Lovat, L. B.</creatorcontrib><creatorcontrib>Novelli, M.</creatorcontrib><creatorcontrib>Haidry, R.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nursing & Allied Health Database</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>Consumer Health Database (Alumni Edition)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Consumer Health Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>Digestive diseases and sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Everson, M.</au><au>Magee, C.</au><au>Alzoubaidi, D.</au><au>Brogden, S.</au><au>Graham, D.</au><au>Lovat, L. B.</au><au>Novelli, M.</au><au>Haidry, R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Minichromosomal Maintenance Component Complex 5 (MCM5) as a Marker of Barrett’s Esophagus-Related Neoplasia: A Feasibility Study</atitle><jtitle>Digestive diseases and sciences</jtitle><stitle>Dig Dis Sci</stitle><addtitle>Dig Dis Sci</addtitle><date>2019-10-01</date><risdate>2019</risdate><volume>64</volume><issue>10</issue><spage>2815</spage><epage>2822</epage><pages>2815-2822</pages><issn>0163-2116</issn><eissn>1573-2568</eissn><abstract>Background
The endoscopic detection of esophageal cancer is suboptimal in both patients referred with dyspeptic symptoms and those enrolled in Barrett’s surveillance programs. MCM5 expression in cells collected from gastric fluid may be correlated with the presence of dysplasia or adenocarcinoma. Analysis of this biomarker may improve the detection of cancer.
Methods
Sixty-one patients were enrolled at a single UK referral center. From each patient, 5–10 ml of gastric fluid was aspirated endoscopically. Patients were categorized according to their histology, normal, non-dysplastic Barrett’s (NDBE), high-grade dysplastic Barrett’s (HGD), and esophageal adenocarcinoma (EAC). All histology was confirmed by Seattle protocol biopsies or endoscopic mucosal resection. Samples were centrifuged, and the cell pellet was lysed. MCM5 expression levels were quantified using a proprietary immunoassay. The mean MCM5 expression was compared between groups by Kruskal–Wallis test. ROC curves were also used to assess diagnostic utility.
Results
The mean expression of MCM5 increases as patients progress from a normal esophagus to NDBE, HGD, and EAC (14.4; 49.8; 112.3; and 154.1, respectively). There was a significant difference in the MCM5 expression of patients with a normal esophagus compared to those with EAC (
p
= 0.04). There was a trend toward higher MCM5 expression in patients with EAC compared to those with NDBE (
p
= 0.34). MCM5 expression was a fair discriminator (AUC 0.70 [95% CI 0.57–0.83]) between patients without neoplasia (normal and NDBE) and those with early neoplasia (HGD and EAC).
Conclusion
MCM5 expression in gastric fluid samples can differentiate patients with a histologically normal esophagus compared to those with early adenocarcinoma. Larger, powered studies are needed to assess whether it can be used to differentiate those with HGD from NDBE.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>30982210</pmid><doi>10.1007/s10620-019-05607-5</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
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| ispartof | Digestive diseases and sciences, 2019-10, Vol.64 (10), p.2815-2822 |
| issn | 0163-2116 1573-2568 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2210007253 |
| source | MEDLINE; SpringerLink Journals |
| subjects | Adenocarcinoma - diagnosis Adenocarcinoma - metabolism Adenocarcinoma - pathology Barrett Esophagus - diagnosis Barrett Esophagus - metabolism Barrett Esophagus - pathology Biochemistry Biomarkers Biomarkers - analysis Biopsy - methods Cancer Cell Cycle Proteins - analysis Cost analysis Deoxyribonucleic acid Diagnosis Disease Progression DNA DNA Replication Dysplasia Early Detection of Cancer - methods Endoscopy Endoscopy, Digestive System - methods Esophageal cancer Esophageal Neoplasms - diagnosis Esophageal Neoplasms - metabolism Esophageal Neoplasms - pathology Feasibility Studies Female Gastric Juice - metabolism Gastroenterology Hepatology Histology Humans Immunoassay Male Medicine Medicine & Public Health Middle Aged Oncology Original Article Pathology Patients Precancerous Conditions - diagnosis Precancerous Conditions - metabolism Precancerous Conditions - pathology Proteins Surveillance Systematic review Transplant Surgery University colleges Urine |
| title | Minichromosomal Maintenance Component Complex 5 (MCM5) as a Marker of Barrett’s Esophagus-Related Neoplasia: A Feasibility Study |
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