A Bacillus pumilus originated β-N-acetylglucosaminidase for chitin combinatory hydrolysis and exploration of its thermostable mechanism

β-N-acetylglucosaminase (NAGase) plays pivotal roles in industrial applications. Here, a GH3 family NAGase encoding gene from Bacillus pumilus was cloned and expressed in Escherichia coli. The optimal temperature and pH of the recombinant BpNagZ were 70 °C and 6.0, respectively, and kept more than 4...

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Veröffentlicht in:International journal of biological macromolecules 2019-07, Vol.132, p.1282-1289
Hauptverfasser: Du, Chao, Jiang, Shun, Jiang, Sijing, Zhou, Yuling, Zhang, Guimin
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Sprache:eng
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