The role of CD44H molecule in the interactions between human monocytes and pancreatic adenocarcinoma-derived microvesicles
CD44H is a transmembrane molecule important for cell-cell and cell-extracellular matrix interactions. In monocytes, CD44H is implicated in phagocytosis of particles coated by hyaluronan (HA). HA fragments were shown to induce chemokine secretion by monocytes. Tumour derived microvesicles (TMVs), whi...
Gespeichert in:
| Veröffentlicht in: | Folia histochemica et cytobiologica 2019-01, Vol.57 (1), p.28-34 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 34 |
|---|---|
| container_issue | 1 |
| container_start_page | 28 |
| container_title | Folia histochemica et cytobiologica |
| container_volume | 57 |
| creator | Baj-Krzyworzeka, Monika Weglarczyk, Kazimierz Szatanek, Rafal Mytar, Bozenna Baran, Jaroslaw Siedlar, Maciej |
| description | CD44H is a transmembrane molecule important for cell-cell and cell-extracellular matrix interactions. In monocytes, CD44H is implicated in phagocytosis of particles coated by hyaluronan (HA). HA fragments were shown to induce chemokine secretion by monocytes. Tumour derived microvesicles (TMVs), which are small membrane fragments derived from tumour cells can carry fragments of HA. The aim of the study was to examine whether monocyte's CD44H is involved in the engulfment of pancreatic adenocarcinoma-derived microvesicles and in the production of chemokines induced by TMVs.
TMVs engulfment and chemokines' secretion stimulated with TMVs were determined in control human monocytes and cells incubated with anti-CD44H monoclonal antibody (mAb) by flow cytometry and ELISA, respectively. Phosphorylation of STAT3, transcription factor essential for chemokines' production and CD44 signal transduction, was determined by Western blotting.
Blocking of CD44H by anti-CD44H mAb on monocytes decreased the engulfment of TMVs and the secretion of CCL4 and CCL5, but had no effect on CCL2, CCL3 and CXCL8. STAT-3 phosphorylation in monocytes incubated with TMVs after CD44 blocking was also reduced.
The results suggest that tumour-derived microvesicles (TMVs) may carry bioactive cargo(s) which induces STAT3 dependent signalling pathway in human monocytes via CD44 molecules. |
| doi_str_mv | 10.5603/FHC.a2019.0005 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2205410564</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2205410564</sourcerecordid><originalsourceid>FETCH-LOGICAL-c351t-90a1b86582625bdf9ee433c1c918290af90965ff411d8306b107b18f804118883</originalsourceid><addsrcrecordid>eNp9kUFvFSEQgImxsc_Wq0dD4sXLvjKwsHA0T-szaeKlPROWnc2j2YUn7NbUXy_PVg8ePE1gPmaY-Qh5C2wrFRNX1_vd1nEGZssYky_IBrTpGqkEvCQbxoVptGT6nLwu5b4CirXwipwLZmSnO9iQn7cHpDlNSNNId5_adk_nevJrvQmRLodTWDA7v4QUC-1x-YEY6WGdXaxoTP5xwUJdHOjRRZ_RLcFTN2DNuOxDTLNrBszhAQc6B5_TA5bgJyyX5Gx0U8E3z_GC3F1_vt3tm5tvX77uPt40XkhYGsMc9FpJzRWX_TAaxFYID96A5jU5GmaUHMcWYNCCqR5Y14MedR0VtNbignx4qnvM6fuKZbFzKB6nyUVMa7GcM9lC3U1b0ff_oPdpzbH-znJleCc6qDv9H8WhNYyBOrXdPlF15FIyjvaYw-zyowVmT-5sdWd_u7Mnd_XBu-eyaz_j8Bf_I0v8AjC_kuk</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2214900168</pqid></control><display><type>article</type><title>The role of CD44H molecule in the interactions between human monocytes and pancreatic adenocarcinoma-derived microvesicles</title><source>EZB-FREE-00999 freely available EZB journals</source><creator>Baj-Krzyworzeka, Monika ; Weglarczyk, Kazimierz ; Szatanek, Rafal ; Mytar, Bozenna ; Baran, Jaroslaw ; Siedlar, Maciej</creator><creatorcontrib>Baj-Krzyworzeka, Monika ; Weglarczyk, Kazimierz ; Szatanek, Rafal ; Mytar, Bozenna ; Baran, Jaroslaw ; Siedlar, Maciej</creatorcontrib><description>CD44H is a transmembrane molecule important for cell-cell and cell-extracellular matrix interactions. In monocytes, CD44H is implicated in phagocytosis of particles coated by hyaluronan (HA). HA fragments were shown to induce chemokine secretion by monocytes. Tumour derived microvesicles (TMVs), which are small membrane fragments derived from tumour cells can carry fragments of HA. The aim of the study was to examine whether monocyte's CD44H is involved in the engulfment of pancreatic adenocarcinoma-derived microvesicles and in the production of chemokines induced by TMVs.
TMVs engulfment and chemokines' secretion stimulated with TMVs were determined in control human monocytes and cells incubated with anti-CD44H monoclonal antibody (mAb) by flow cytometry and ELISA, respectively. Phosphorylation of STAT3, transcription factor essential for chemokines' production and CD44 signal transduction, was determined by Western blotting.
Blocking of CD44H by anti-CD44H mAb on monocytes decreased the engulfment of TMVs and the secretion of CCL4 and CCL5, but had no effect on CCL2, CCL3 and CXCL8. STAT-3 phosphorylation in monocytes incubated with TMVs after CD44 blocking was also reduced.
The results suggest that tumour-derived microvesicles (TMVs) may carry bioactive cargo(s) which induces STAT3 dependent signalling pathway in human monocytes via CD44 molecules.</description><identifier>ISSN: 0239-8508</identifier><identifier>EISSN: 1897-5631</identifier><identifier>DOI: 10.5603/FHC.a2019.0005</identifier><identifier>PMID: 30957871</identifier><language>eng</language><publisher>Poland: Wydawnictwo Via Medica</publisher><subject>Adenocarcinoma ; Biomedical materials ; Cancer ; Carbon tetrachloride ; CCL3 protein ; CCL4 protein ; CD44 antigen ; Chemokines ; Cytokines ; Enzyme-linked immunosorbent assay ; Extracellular matrix ; Flow cytometry ; Fragmentation ; Fragments ; Hyaluronic acid ; Laboratories ; Metastasis ; Molecular weight ; Monoclonal antibodies ; Monocyte chemoattractant protein 1 ; Monocytes ; Pancreas ; Pancreatic cancer ; Phagocytosis ; Phosphorylation ; Signal transduction ; Stat3 protein ; Stem cells ; Tumors ; Western blotting</subject><ispartof>Folia histochemica et cytobiologica, 2019-01, Vol.57 (1), p.28-34</ispartof><rights>2019. This work is published under https://creativecommons.org/licenses/by-nc-nd/4.0 (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2019. This work is published under https://creativecommons.org/licenses/by-nc-nd/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c351t-90a1b86582625bdf9ee433c1c918290af90965ff411d8306b107b18f804118883</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27915,27916</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30957871$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Baj-Krzyworzeka, Monika</creatorcontrib><creatorcontrib>Weglarczyk, Kazimierz</creatorcontrib><creatorcontrib>Szatanek, Rafal</creatorcontrib><creatorcontrib>Mytar, Bozenna</creatorcontrib><creatorcontrib>Baran, Jaroslaw</creatorcontrib><creatorcontrib>Siedlar, Maciej</creatorcontrib><title>The role of CD44H molecule in the interactions between human monocytes and pancreatic adenocarcinoma-derived microvesicles</title><title>Folia histochemica et cytobiologica</title><addtitle>Folia Histochem Cytobiol</addtitle><description>CD44H is a transmembrane molecule important for cell-cell and cell-extracellular matrix interactions. In monocytes, CD44H is implicated in phagocytosis of particles coated by hyaluronan (HA). HA fragments were shown to induce chemokine secretion by monocytes. Tumour derived microvesicles (TMVs), which are small membrane fragments derived from tumour cells can carry fragments of HA. The aim of the study was to examine whether monocyte's CD44H is involved in the engulfment of pancreatic adenocarcinoma-derived microvesicles and in the production of chemokines induced by TMVs.
TMVs engulfment and chemokines' secretion stimulated with TMVs were determined in control human monocytes and cells incubated with anti-CD44H monoclonal antibody (mAb) by flow cytometry and ELISA, respectively. Phosphorylation of STAT3, transcription factor essential for chemokines' production and CD44 signal transduction, was determined by Western blotting.
Blocking of CD44H by anti-CD44H mAb on monocytes decreased the engulfment of TMVs and the secretion of CCL4 and CCL5, but had no effect on CCL2, CCL3 and CXCL8. STAT-3 phosphorylation in monocytes incubated with TMVs after CD44 blocking was also reduced.
The results suggest that tumour-derived microvesicles (TMVs) may carry bioactive cargo(s) which induces STAT3 dependent signalling pathway in human monocytes via CD44 molecules.</description><subject>Adenocarcinoma</subject><subject>Biomedical materials</subject><subject>Cancer</subject><subject>Carbon tetrachloride</subject><subject>CCL3 protein</subject><subject>CCL4 protein</subject><subject>CD44 antigen</subject><subject>Chemokines</subject><subject>Cytokines</subject><subject>Enzyme-linked immunosorbent assay</subject><subject>Extracellular matrix</subject><subject>Flow cytometry</subject><subject>Fragmentation</subject><subject>Fragments</subject><subject>Hyaluronic acid</subject><subject>Laboratories</subject><subject>Metastasis</subject><subject>Molecular weight</subject><subject>Monoclonal antibodies</subject><subject>Monocyte chemoattractant protein 1</subject><subject>Monocytes</subject><subject>Pancreas</subject><subject>Pancreatic cancer</subject><subject>Phagocytosis</subject><subject>Phosphorylation</subject><subject>Signal transduction</subject><subject>Stat3 protein</subject><subject>Stem cells</subject><subject>Tumors</subject><subject>Western blotting</subject><issn>0239-8508</issn><issn>1897-5631</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNp9kUFvFSEQgImxsc_Wq0dD4sXLvjKwsHA0T-szaeKlPROWnc2j2YUn7NbUXy_PVg8ePE1gPmaY-Qh5C2wrFRNX1_vd1nEGZssYky_IBrTpGqkEvCQbxoVptGT6nLwu5b4CirXwipwLZmSnO9iQn7cHpDlNSNNId5_adk_nevJrvQmRLodTWDA7v4QUC-1x-YEY6WGdXaxoTP5xwUJdHOjRRZ_RLcFTN2DNuOxDTLNrBszhAQc6B5_TA5bgJyyX5Gx0U8E3z_GC3F1_vt3tm5tvX77uPt40XkhYGsMc9FpJzRWX_TAaxFYID96A5jU5GmaUHMcWYNCCqR5Y14MedR0VtNbignx4qnvM6fuKZbFzKB6nyUVMa7GcM9lC3U1b0ff_oPdpzbH-znJleCc6qDv9H8WhNYyBOrXdPlF15FIyjvaYw-zyowVmT-5sdWd_u7Mnd_XBu-eyaz_j8Bf_I0v8AjC_kuk</recordid><startdate>20190101</startdate><enddate>20190101</enddate><creator>Baj-Krzyworzeka, Monika</creator><creator>Weglarczyk, Kazimierz</creator><creator>Szatanek, Rafal</creator><creator>Mytar, Bozenna</creator><creator>Baran, Jaroslaw</creator><creator>Siedlar, Maciej</creator><general>Wydawnictwo Via Medica</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>4T-</scope><scope>7QL</scope><scope>7QP</scope><scope>7T5</scope><scope>7TK</scope><scope>7TO</scope><scope>7U7</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>BYOGL</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M7P</scope><scope>MBDVC</scope><scope>P64</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20190101</creationdate><title>The role of CD44H molecule in the interactions between human monocytes and pancreatic adenocarcinoma-derived microvesicles</title><author>Baj-Krzyworzeka, Monika ; Weglarczyk, Kazimierz ; Szatanek, Rafal ; Mytar, Bozenna ; Baran, Jaroslaw ; Siedlar, Maciej</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c351t-90a1b86582625bdf9ee433c1c918290af90965ff411d8306b107b18f804118883</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Adenocarcinoma</topic><topic>Biomedical materials</topic><topic>Cancer</topic><topic>Carbon tetrachloride</topic><topic>CCL3 protein</topic><topic>CCL4 protein</topic><topic>CD44 antigen</topic><topic>Chemokines</topic><topic>Cytokines</topic><topic>Enzyme-linked immunosorbent assay</topic><topic>Extracellular matrix</topic><topic>Flow cytometry</topic><topic>Fragmentation</topic><topic>Fragments</topic><topic>Hyaluronic acid</topic><topic>Laboratories</topic><topic>Metastasis</topic><topic>Molecular weight</topic><topic>Monoclonal antibodies</topic><topic>Monocyte chemoattractant protein 1</topic><topic>Monocytes</topic><topic>Pancreas</topic><topic>Pancreatic cancer</topic><topic>Phagocytosis</topic><topic>Phosphorylation</topic><topic>Signal transduction</topic><topic>Stat3 protein</topic><topic>Stem cells</topic><topic>Tumors</topic><topic>Western blotting</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Baj-Krzyworzeka, Monika</creatorcontrib><creatorcontrib>Weglarczyk, Kazimierz</creatorcontrib><creatorcontrib>Szatanek, Rafal</creatorcontrib><creatorcontrib>Mytar, Bozenna</creatorcontrib><creatorcontrib>Baran, Jaroslaw</creatorcontrib><creatorcontrib>Siedlar, Maciej</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Docstoc</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Immunology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Toxicology Abstracts</collection><collection>ProQuest Health and Medical</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>East Europe, Central Europe Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest Research Library</collection><collection>ProQuest Biological Science Journals</collection><collection>Research Library (Corporate)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Folia histochemica et cytobiologica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Baj-Krzyworzeka, Monika</au><au>Weglarczyk, Kazimierz</au><au>Szatanek, Rafal</au><au>Mytar, Bozenna</au><au>Baran, Jaroslaw</au><au>Siedlar, Maciej</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The role of CD44H molecule in the interactions between human monocytes and pancreatic adenocarcinoma-derived microvesicles</atitle><jtitle>Folia histochemica et cytobiologica</jtitle><addtitle>Folia Histochem Cytobiol</addtitle><date>2019-01-01</date><risdate>2019</risdate><volume>57</volume><issue>1</issue><spage>28</spage><epage>34</epage><pages>28-34</pages><issn>0239-8508</issn><eissn>1897-5631</eissn><abstract>CD44H is a transmembrane molecule important for cell-cell and cell-extracellular matrix interactions. In monocytes, CD44H is implicated in phagocytosis of particles coated by hyaluronan (HA). HA fragments were shown to induce chemokine secretion by monocytes. Tumour derived microvesicles (TMVs), which are small membrane fragments derived from tumour cells can carry fragments of HA. The aim of the study was to examine whether monocyte's CD44H is involved in the engulfment of pancreatic adenocarcinoma-derived microvesicles and in the production of chemokines induced by TMVs.
TMVs engulfment and chemokines' secretion stimulated with TMVs were determined in control human monocytes and cells incubated with anti-CD44H monoclonal antibody (mAb) by flow cytometry and ELISA, respectively. Phosphorylation of STAT3, transcription factor essential for chemokines' production and CD44 signal transduction, was determined by Western blotting.
Blocking of CD44H by anti-CD44H mAb on monocytes decreased the engulfment of TMVs and the secretion of CCL4 and CCL5, but had no effect on CCL2, CCL3 and CXCL8. STAT-3 phosphorylation in monocytes incubated with TMVs after CD44 blocking was also reduced.
The results suggest that tumour-derived microvesicles (TMVs) may carry bioactive cargo(s) which induces STAT3 dependent signalling pathway in human monocytes via CD44 molecules.</abstract><cop>Poland</cop><pub>Wydawnictwo Via Medica</pub><pmid>30957871</pmid><doi>10.5603/FHC.a2019.0005</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0239-8508 |
| ispartof | Folia histochemica et cytobiologica, 2019-01, Vol.57 (1), p.28-34 |
| issn | 0239-8508 1897-5631 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2205410564 |
| source | EZB-FREE-00999 freely available EZB journals |
| subjects | Adenocarcinoma Biomedical materials Cancer Carbon tetrachloride CCL3 protein CCL4 protein CD44 antigen Chemokines Cytokines Enzyme-linked immunosorbent assay Extracellular matrix Flow cytometry Fragmentation Fragments Hyaluronic acid Laboratories Metastasis Molecular weight Monoclonal antibodies Monocyte chemoattractant protein 1 Monocytes Pancreas Pancreatic cancer Phagocytosis Phosphorylation Signal transduction Stat3 protein Stem cells Tumors Western blotting |
| title | The role of CD44H molecule in the interactions between human monocytes and pancreatic adenocarcinoma-derived microvesicles |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-14T23%3A58%3A27IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=The%20role%20of%20CD44H%20molecule%20in%20the%20interactions%20between%20human%20monocytes%20and%20pancreatic%20adenocarcinoma-derived%20microvesicles&rft.jtitle=Folia%20histochemica%20et%20cytobiologica&rft.au=Baj-Krzyworzeka,%20Monika&rft.date=2019-01-01&rft.volume=57&rft.issue=1&rft.spage=28&rft.epage=34&rft.pages=28-34&rft.issn=0239-8508&rft.eissn=1897-5631&rft_id=info:doi/10.5603/FHC.a2019.0005&rft_dat=%3Cproquest_cross%3E2205410564%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2214900168&rft_id=info:pmid/30957871&rfr_iscdi=true |