Quantification of viable bacterial load in artificial sputum spiked with Mycobacterium tuberculosis

Artificial sputum spiked with Mycobacterium tuberculosis could serve for validation of procedures that determine viable mycobacterial load. Artificial sputum specimens prepared in-house were spiked with low, medium or high concentrations of Mycobacterium tuberculosis H37Rv stock solution. In a first...

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Veröffentlicht in:	Tuberculosis (Edinburgh, Scotland) Scotland), 2019-03, Vol.115, p.140-145
Hauptverfasser:	Friedrich, Sven O., Kolwijck, Eva, Karinja, Miriam N., van der Merwe, Lize, Diacon, Andreas H.
Format:	Artikel
Sprache:	eng
Schlagworte:	Artificial sputum Bacterial Load Culture Media - chemistry Feasibility Studies Mycobacterium tuberculosis Mycobacterium tuberculosis - isolation & purification Proficiency Reproducibility of Results Specimen Handling Sputum Sputum - microbiology Storage Storage temperature Technologists Tuberculosis Validation Variance Viability
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