Undernourishment and recurrent seizures early in life impair Long-Term Potentiation and alter NMDAR and AMPAR expression in rat hippocampus
•Animals exposed to undernourishment or recurrent seizures failed to promote LTP after stimulation.•Seizure exposure early in life leads to increased expression of hippocampal NR1A, NR2A, NR2B, NR2C and NR2D.•When animals are exposed to undernourishment paradigm early in life, upregulation of NDMA s...
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| Veröffentlicht in: | International journal of developmental neuroscience 2019-06, Vol.75 (1), p.13-18 |
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| creator | Wearick-Silva, L.E. Sebben, A.D. Costa-Ferro, Z.S.M. Marinowic, D.R. Nunes, M.L. |
| description | •Animals exposed to undernourishment or recurrent seizures failed to promote LTP after stimulation.•Seizure exposure early in life leads to increased expression of hippocampal NR1A, NR2A, NR2B, NR2C and NR2D.•When animals are exposed to undernourishment paradigm early in life, upregulation of NDMA subunits was absent.
Undernourishment is a global issue, especially in developing countries, affecting newborns and children in a vulnerable period of brain development. Previous studies of undernourishment models suggested a relationship between undernourishment and epilepsy. The exposure to both undernourishment and recurrent seizures early in life appears to have detrimental effects on the developing brain. This study aims to investigate the neurobiological consequences of undernourishment and recurrent seizures exposure early in life, investigating Long-Term Potentiation (LTP) induction and gene expression of NMDA receptor subunits in the hippocampus during adulthood (P60). Animals were exposed to maternal deprivation protocol from P2 to P15 to control food intake in rat pups and Flurothyl-induced seizures from P7 to P10. Electrophysiological records of hippocampal slices were recorded and gene expression of NR1A, NR2A, NR2B, NR2C, NR2D and BDNF were investigated. Animals exposed to undernourishment or recurrent seizures failed to promote LTP after stimulation. Furthermore, seizure exposure early in life led to increased expression of hippocampal NR1A, NR2A, NR2B, NR2C and NR2D when compared to controls. Interestingly, when animals were exposed to undernourishment paradigm early in life, this upregulation of NDMA subunits was absent. In conclusion, our study showed impaired LTP after undernourishment and recurrent seizures early in life, together with differential expression of NDMA expression in the hippocampus during adulthood. |
| doi_str_mv | 10.1016/j.ijdevneu.2019.03.005 |
| format | Article |
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Undernourishment is a global issue, especially in developing countries, affecting newborns and children in a vulnerable period of brain development. Previous studies of undernourishment models suggested a relationship between undernourishment and epilepsy. The exposure to both undernourishment and recurrent seizures early in life appears to have detrimental effects on the developing brain. This study aims to investigate the neurobiological consequences of undernourishment and recurrent seizures exposure early in life, investigating Long-Term Potentiation (LTP) induction and gene expression of NMDA receptor subunits in the hippocampus during adulthood (P60). Animals were exposed to maternal deprivation protocol from P2 to P15 to control food intake in rat pups and Flurothyl-induced seizures from P7 to P10. Electrophysiological records of hippocampal slices were recorded and gene expression of NR1A, NR2A, NR2B, NR2C, NR2D and BDNF were investigated. Animals exposed to undernourishment or recurrent seizures failed to promote LTP after stimulation. Furthermore, seizure exposure early in life led to increased expression of hippocampal NR1A, NR2A, NR2B, NR2C and NR2D when compared to controls. Interestingly, when animals were exposed to undernourishment paradigm early in life, this upregulation of NDMA subunits was absent. In conclusion, our study showed impaired LTP after undernourishment and recurrent seizures early in life, together with differential expression of NDMA expression in the hippocampus during adulthood.</description><identifier>ISSN: 0736-5748</identifier><identifier>EISSN: 1873-474X</identifier><identifier>DOI: 10.1016/j.ijdevneu.2019.03.005</identifier><identifier>PMID: 30940500</identifier><language>eng</language><publisher>United States: Elsevier Ltd</publisher><subject>Animals ; Brain ; Brain slice preparation ; Brain-derived neurotrophic factor ; Brain-Derived Neurotrophic Factor - genetics ; Brain-Derived Neurotrophic Factor - metabolism ; Convulsions & seizures ; Deprivation ; Developing countries ; Epilepsy ; Exposure ; Flurothyl ; Food intake ; Gene Expression ; Glutamic acid receptors (ionotropic) ; Hippocampus ; Hippocampus - metabolism ; LDCs ; Long-Term Potentiation ; Long-Term Potentiation - physiology ; Malnutrition - metabolism ; Malnutrition - physiopathology ; Maternal Deprivation ; N-Methyl-D-aspartic acid receptors ; Neonates ; NMDA receptors ; Rats ; Receptors, AMPA - genetics ; Receptors, AMPA - metabolism ; Receptors, N-Methyl-D-Aspartate - genetics ; Receptors, N-Methyl-D-Aspartate - metabolism ; Seizures ; Seizures - chemically induced ; Seizures - metabolism ; Seizures - physiopathology ; Undernourishment ; α-Amino-3-hydroxy-5-methyl-4-isoxazole propionic acid receptors</subject><ispartof>International journal of developmental neuroscience, 2019-06, Vol.75 (1), p.13-18</ispartof><rights>2019 ISDN</rights><rights>Copyright © 2019 ISDN. Published by Elsevier Ltd. All rights reserved.</rights><rights>Copyright Elsevier BV Jun 2019</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4625-fb546c269ccad0cde3401d140fee2234c886d57023e9103eba04875ecb5e40973</citedby><cites>FETCH-LOGICAL-c4625-fb546c269ccad0cde3401d140fee2234c886d57023e9103eba04875ecb5e40973</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1016%2Fj.ijdevneu.2019.03.005$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1016%2Fj.ijdevneu.2019.03.005$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30940500$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wearick-Silva, L.E.</creatorcontrib><creatorcontrib>Sebben, A.D.</creatorcontrib><creatorcontrib>Costa-Ferro, Z.S.M.</creatorcontrib><creatorcontrib>Marinowic, D.R.</creatorcontrib><creatorcontrib>Nunes, M.L.</creatorcontrib><title>Undernourishment and recurrent seizures early in life impair Long-Term Potentiation and alter NMDAR and AMPAR expression in rat hippocampus</title><title>International journal of developmental neuroscience</title><addtitle>Int J Dev Neurosci</addtitle><description>•Animals exposed to undernourishment or recurrent seizures failed to promote LTP after stimulation.•Seizure exposure early in life leads to increased expression of hippocampal NR1A, NR2A, NR2B, NR2C and NR2D.•When animals are exposed to undernourishment paradigm early in life, upregulation of NDMA subunits was absent.
Undernourishment is a global issue, especially in developing countries, affecting newborns and children in a vulnerable period of brain development. Previous studies of undernourishment models suggested a relationship between undernourishment and epilepsy. The exposure to both undernourishment and recurrent seizures early in life appears to have detrimental effects on the developing brain. This study aims to investigate the neurobiological consequences of undernourishment and recurrent seizures exposure early in life, investigating Long-Term Potentiation (LTP) induction and gene expression of NMDA receptor subunits in the hippocampus during adulthood (P60). Animals were exposed to maternal deprivation protocol from P2 to P15 to control food intake in rat pups and Flurothyl-induced seizures from P7 to P10. Electrophysiological records of hippocampal slices were recorded and gene expression of NR1A, NR2A, NR2B, NR2C, NR2D and BDNF were investigated. Animals exposed to undernourishment or recurrent seizures failed to promote LTP after stimulation. Furthermore, seizure exposure early in life led to increased expression of hippocampal NR1A, NR2A, NR2B, NR2C and NR2D when compared to controls. Interestingly, when animals were exposed to undernourishment paradigm early in life, this upregulation of NDMA subunits was absent. In conclusion, our study showed impaired LTP after undernourishment and recurrent seizures early in life, together with differential expression of NDMA expression in the hippocampus during adulthood.</description><subject>Animals</subject><subject>Brain</subject><subject>Brain slice preparation</subject><subject>Brain-derived neurotrophic factor</subject><subject>Brain-Derived Neurotrophic Factor - genetics</subject><subject>Brain-Derived Neurotrophic Factor - metabolism</subject><subject>Convulsions & seizures</subject><subject>Deprivation</subject><subject>Developing countries</subject><subject>Epilepsy</subject><subject>Exposure</subject><subject>Flurothyl</subject><subject>Food intake</subject><subject>Gene Expression</subject><subject>Glutamic acid receptors (ionotropic)</subject><subject>Hippocampus</subject><subject>Hippocampus - metabolism</subject><subject>LDCs</subject><subject>Long-Term Potentiation</subject><subject>Long-Term Potentiation - physiology</subject><subject>Malnutrition - metabolism</subject><subject>Malnutrition - physiopathology</subject><subject>Maternal Deprivation</subject><subject>N-Methyl-D-aspartic acid receptors</subject><subject>Neonates</subject><subject>NMDA receptors</subject><subject>Rats</subject><subject>Receptors, AMPA - genetics</subject><subject>Receptors, AMPA - metabolism</subject><subject>Receptors, N-Methyl-D-Aspartate - genetics</subject><subject>Receptors, N-Methyl-D-Aspartate - metabolism</subject><subject>Seizures</subject><subject>Seizures - chemically induced</subject><subject>Seizures - metabolism</subject><subject>Seizures - physiopathology</subject><subject>Undernourishment</subject><subject>α-Amino-3-hydroxy-5-methyl-4-isoxazole propionic acid receptors</subject><issn>0736-5748</issn><issn>1873-474X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc9u1DAQhyMEokvhFapIXLgkTGI7Tm6sugvd1XapUBdxs7zOhHqVOKmdlC6vwEvjNC0HLnDyH33zzWh-QXCWQJxAkr0_xPpQ4p3BIU4hKWIgMQB7FsySnJOIcvrteTADTrKIcZqfBK-cO4AnGNCXwQmBggIDmAW_dqZEa9rBanfToOlDacrQohqsHV8O9c_BogtR2voYahPWusJQN53UNty05nt0jbYJr9re41r2ujUPCln3aMPt5WL-5eE9v7zyN7zvvMyNkFdZ2Yc3uutaJZtucK-DF5WsHb55PE-D3cfl9flFtPn8aXU-30SKZimLqj2jmUqzQilZgiqRUEjKhEKFmKaEqjzPSsYhJVgkQHAvgeacodozpFBwchq8m7ydbW8HdL1otFNY19JgOziRppBm3DcgHn37F3rwqzJ-Ok-N03DGM09lE6Vs65zFSnRWN9IeRQJijEscxFNcYoxLABE-DF949qgf9g2Wf8qe8vHAagJ-6BqP_6kV68V2vVovll-3y934D2Rq9mFyod_tnUYrnNJoFJba592LstX_mvc3LHjBfg</recordid><startdate>201906</startdate><enddate>201906</enddate><creator>Wearick-Silva, L.E.</creator><creator>Sebben, A.D.</creator><creator>Costa-Ferro, Z.S.M.</creator><creator>Marinowic, D.R.</creator><creator>Nunes, M.L.</creator><general>Elsevier Ltd</general><general>Elsevier BV</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QR</scope><scope>7TK</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>201906</creationdate><title>Undernourishment and recurrent seizures early in life impair Long-Term Potentiation and alter NMDAR and AMPAR expression in rat hippocampus</title><author>Wearick-Silva, L.E. ; Sebben, A.D. ; Costa-Ferro, Z.S.M. ; Marinowic, D.R. ; Nunes, M.L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4625-fb546c269ccad0cde3401d140fee2234c886d57023e9103eba04875ecb5e40973</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Animals</topic><topic>Brain</topic><topic>Brain slice preparation</topic><topic>Brain-derived neurotrophic factor</topic><topic>Brain-Derived Neurotrophic Factor - genetics</topic><topic>Brain-Derived Neurotrophic Factor - metabolism</topic><topic>Convulsions & seizures</topic><topic>Deprivation</topic><topic>Developing countries</topic><topic>Epilepsy</topic><topic>Exposure</topic><topic>Flurothyl</topic><topic>Food intake</topic><topic>Gene Expression</topic><topic>Glutamic acid receptors (ionotropic)</topic><topic>Hippocampus</topic><topic>Hippocampus - metabolism</topic><topic>LDCs</topic><topic>Long-Term Potentiation</topic><topic>Long-Term Potentiation - physiology</topic><topic>Malnutrition - metabolism</topic><topic>Malnutrition - physiopathology</topic><topic>Maternal Deprivation</topic><topic>N-Methyl-D-aspartic acid receptors</topic><topic>Neonates</topic><topic>NMDA receptors</topic><topic>Rats</topic><topic>Receptors, AMPA - genetics</topic><topic>Receptors, AMPA - metabolism</topic><topic>Receptors, N-Methyl-D-Aspartate - genetics</topic><topic>Receptors, N-Methyl-D-Aspartate - metabolism</topic><topic>Seizures</topic><topic>Seizures - chemically induced</topic><topic>Seizures - metabolism</topic><topic>Seizures - physiopathology</topic><topic>Undernourishment</topic><topic>α-Amino-3-hydroxy-5-methyl-4-isoxazole propionic acid receptors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wearick-Silva, L.E.</creatorcontrib><creatorcontrib>Sebben, A.D.</creatorcontrib><creatorcontrib>Costa-Ferro, Z.S.M.</creatorcontrib><creatorcontrib>Marinowic, D.R.</creatorcontrib><creatorcontrib>Nunes, M.L.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>International journal of developmental neuroscience</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wearick-Silva, L.E.</au><au>Sebben, A.D.</au><au>Costa-Ferro, Z.S.M.</au><au>Marinowic, D.R.</au><au>Nunes, M.L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Undernourishment and recurrent seizures early in life impair Long-Term Potentiation and alter NMDAR and AMPAR expression in rat hippocampus</atitle><jtitle>International journal of developmental neuroscience</jtitle><addtitle>Int J Dev Neurosci</addtitle><date>2019-06</date><risdate>2019</risdate><volume>75</volume><issue>1</issue><spage>13</spage><epage>18</epage><pages>13-18</pages><issn>0736-5748</issn><eissn>1873-474X</eissn><abstract>•Animals exposed to undernourishment or recurrent seizures failed to promote LTP after stimulation.•Seizure exposure early in life leads to increased expression of hippocampal NR1A, NR2A, NR2B, NR2C and NR2D.•When animals are exposed to undernourishment paradigm early in life, upregulation of NDMA subunits was absent.
Undernourishment is a global issue, especially in developing countries, affecting newborns and children in a vulnerable period of brain development. Previous studies of undernourishment models suggested a relationship between undernourishment and epilepsy. The exposure to both undernourishment and recurrent seizures early in life appears to have detrimental effects on the developing brain. This study aims to investigate the neurobiological consequences of undernourishment and recurrent seizures exposure early in life, investigating Long-Term Potentiation (LTP) induction and gene expression of NMDA receptor subunits in the hippocampus during adulthood (P60). Animals were exposed to maternal deprivation protocol from P2 to P15 to control food intake in rat pups and Flurothyl-induced seizures from P7 to P10. Electrophysiological records of hippocampal slices were recorded and gene expression of NR1A, NR2A, NR2B, NR2C, NR2D and BDNF were investigated. Animals exposed to undernourishment or recurrent seizures failed to promote LTP after stimulation. Furthermore, seizure exposure early in life led to increased expression of hippocampal NR1A, NR2A, NR2B, NR2C and NR2D when compared to controls. Interestingly, when animals were exposed to undernourishment paradigm early in life, this upregulation of NDMA subunits was absent. In conclusion, our study showed impaired LTP after undernourishment and recurrent seizures early in life, together with differential expression of NDMA expression in the hippocampus during adulthood.</abstract><cop>United States</cop><pub>Elsevier Ltd</pub><pmid>30940500</pmid><doi>10.1016/j.ijdevneu.2019.03.005</doi><tpages>6</tpages></addata></record> |
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| subjects | Animals Brain Brain slice preparation Brain-derived neurotrophic factor Brain-Derived Neurotrophic Factor - genetics Brain-Derived Neurotrophic Factor - metabolism Convulsions & seizures Deprivation Developing countries Epilepsy Exposure Flurothyl Food intake Gene Expression Glutamic acid receptors (ionotropic) Hippocampus Hippocampus - metabolism LDCs Long-Term Potentiation Long-Term Potentiation - physiology Malnutrition - metabolism Malnutrition - physiopathology Maternal Deprivation N-Methyl-D-aspartic acid receptors Neonates NMDA receptors Rats Receptors, AMPA - genetics Receptors, AMPA - metabolism Receptors, N-Methyl-D-Aspartate - genetics Receptors, N-Methyl-D-Aspartate - metabolism Seizures Seizures - chemically induced Seizures - metabolism Seizures - physiopathology Undernourishment α-Amino-3-hydroxy-5-methyl-4-isoxazole propionic acid receptors |
| title | Undernourishment and recurrent seizures early in life impair Long-Term Potentiation and alter NMDAR and AMPAR expression in rat hippocampus |
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