Homeobox D10, a tumor suppressor, inhibits the proliferation and migration of esophageal squamous cell carcinoma
Esophageal squamous cell carcinoma (ESCC) is one of the most common types of esophageal cancer, which is the sixth leading cause of cancer death globally. Homeobox D10 (HOXD10) is a member of the homeobox (HOX) gene family and has been reported to act as a tumor suppressor. However, the potential ro...
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| Veröffentlicht in: | Journal of cellular biochemistry 2019-08, Vol.120 (8), p.13717-13725 |
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| creator | Zhang, Jin Liu, Shiyuan Zhang, Danjie Ma, Zhenchuan Sun, Liangzhang |
| description | Esophageal squamous cell carcinoma (ESCC) is one of the most common types of esophageal cancer, which is the sixth leading cause of cancer death globally. Homeobox D10 (HOXD10) is a member of the homeobox (HOX) gene family and has been reported to act as a tumor suppressor. However, the potential role of HOXD10 in ESCC has not been reported. Thus, the aim of this study was to examine the expression and function of HOXD10 in ESCC. The expressions of HOXD10 in human ESCC tissues and cell lines were detected by quantitative reverse transcription polymerase chain reaction and Western blot. The HOXD10 overexpressing cell lines were established, then CCK‐8 and Transwell assays were performed to examine cell proliferation, migration, and invasion, respectively. The expression of EMT‐related proteins and signaling pathway‐related proteins were detected by Western blot. Our results showed that HOXD10 is lowly expressed in ESCC tissues as well as in ESCC cell lines. Ectopic overexpression of HOXD10 inhibited cell proliferation, migration, and invasion of ESCC cells (P |
| doi_str_mv | 10.1002/jcb.28644 |
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Overexpression of HOXD10 suppressed the proliferation, migration, and invasion via regulating the PI3K/AKT/mTOR signaling pathway in ESCC cells. Thus, targeting HOXD10 may be considered as a therapeutic strategy for ESCC treatment.</description><identifier>ISSN: 0730-2312</identifier><identifier>EISSN: 1097-4644</identifier><identifier>DOI: 10.1002/jcb.28644</identifier><identifier>PMID: 30938888</identifier><language>eng</language><publisher>United States: Wiley Subscription Services, Inc</publisher><subject>1-Phosphatidylinositol 3-kinase ; AKT protein ; Biotechnology ; Cancer ; Cell growth ; Cell migration ; Cell proliferation ; Cholecystokinin ; Ectopic expression ; epithelial‐mesenchymal transition (EMT) ; Esophageal cancer ; esophageal squamous cell carcinoma (ESCC) ; Esophagus ; Growth factors ; Homeobox ; Homeobox D10 (HOXD10) ; In vivo methods and tests ; Insulin ; invasion ; Kinases ; Mesenchyme ; migration ; PI3K/AKT/mTOR signaling pathway ; Polymerase chain reaction ; Proteins ; Reverse transcription ; Signal transduction ; Signaling ; Squamous cell carcinoma ; TOR protein ; Tumor suppressor genes ; Tumors</subject><ispartof>Journal of cellular biochemistry, 2019-08, Vol.120 (8), p.13717-13725</ispartof><rights>2019 Wiley Periodicals, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3534-504ae0676230a9fa84ac6b55be073a0217a3830d133d0d276ace86ea464c97cf3</citedby><cites>FETCH-LOGICAL-c3534-504ae0676230a9fa84ac6b55be073a0217a3830d133d0d276ace86ea464c97cf3</cites><orcidid>0000-0003-0093-4121</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjcb.28644$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjcb.28644$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30938888$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhang, Jin</creatorcontrib><creatorcontrib>Liu, Shiyuan</creatorcontrib><creatorcontrib>Zhang, Danjie</creatorcontrib><creatorcontrib>Ma, Zhenchuan</creatorcontrib><creatorcontrib>Sun, Liangzhang</creatorcontrib><title>Homeobox D10, a tumor suppressor, inhibits the proliferation and migration of esophageal squamous cell carcinoma</title><title>Journal of cellular biochemistry</title><addtitle>J Cell Biochem</addtitle><description>Esophageal squamous cell carcinoma (ESCC) is one of the most common types of esophageal cancer, which is the sixth leading cause of cancer death globally. Homeobox D10 (HOXD10) is a member of the homeobox (HOX) gene family and has been reported to act as a tumor suppressor. However, the potential role of HOXD10 in ESCC has not been reported. Thus, the aim of this study was to examine the expression and function of HOXD10 in ESCC. The expressions of HOXD10 in human ESCC tissues and cell lines were detected by quantitative reverse transcription polymerase chain reaction and Western blot. The HOXD10 overexpressing cell lines were established, then CCK‐8 and Transwell assays were performed to examine cell proliferation, migration, and invasion, respectively. The expression of EMT‐related proteins and signaling pathway‐related proteins were detected by Western blot. Our results showed that HOXD10 is lowly expressed in ESCC tissues as well as in ESCC cell lines. Ectopic overexpression of HOXD10 inhibited cell proliferation, migration, and invasion of ESCC cells (P < 0.05). HOXD10 overexpression repressed the epithelial‐mesenchymal transition (EMT) process in ESCC cells. Besides, HOXD10 overexpression suppressed the activation of PI3K/AKT/mTOR signaling pathway. PI3K/Akt agonist, insulin‐like growth factor‐1, reversed the inhibitory effects of HOXD10 on cell proliferation and migration in ESCC cells. Additional in vivo study proved that ectopic expression of HOXD10 caused an obvious inhibitory effect on the tumor growth. These findings indicated that overexpression of HOXD10 suppressed the proliferation, migration, and invasion via regulating the PI3K/AKT/mTOR signaling pathway in ESCC cells. Thus, targeting HOXD10 may be considered as a therapeutic strategy for ESCC treatment.
Overexpression of HOXD10 suppressed the proliferation, migration, and invasion via regulating the PI3K/AKT/mTOR signaling pathway in ESCC cells. Thus, targeting HOXD10 may be considered as a therapeutic strategy for ESCC treatment.</description><subject>1-Phosphatidylinositol 3-kinase</subject><subject>AKT protein</subject><subject>Biotechnology</subject><subject>Cancer</subject><subject>Cell growth</subject><subject>Cell migration</subject><subject>Cell proliferation</subject><subject>Cholecystokinin</subject><subject>Ectopic expression</subject><subject>epithelial‐mesenchymal transition (EMT)</subject><subject>Esophageal cancer</subject><subject>esophageal squamous cell carcinoma (ESCC)</subject><subject>Esophagus</subject><subject>Growth factors</subject><subject>Homeobox</subject><subject>Homeobox D10 (HOXD10)</subject><subject>In vivo methods and tests</subject><subject>Insulin</subject><subject>invasion</subject><subject>Kinases</subject><subject>Mesenchyme</subject><subject>migration</subject><subject>PI3K/AKT/mTOR signaling pathway</subject><subject>Polymerase chain reaction</subject><subject>Proteins</subject><subject>Reverse transcription</subject><subject>Signal transduction</subject><subject>Signaling</subject><subject>Squamous cell carcinoma</subject><subject>TOR protein</subject><subject>Tumor suppressor genes</subject><subject>Tumors</subject><issn>0730-2312</issn><issn>1097-4644</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNp1kU1P3DAQhi1UxG63HPgDyFIvrbSBsZ3PI91-AELiUs7RxJnsepXEwU7U8u_xsgsHJHyxLT169M68jJ0JuBAA8nKrqwuZp3F8xOYCiiyKw_sTm0OmIJJKyBn77P0WAIpCyRM2U1CoPJw5G65tR7ay__lPAUuOfJw667ifhsGR99Ytuek3pjKj5-OG-OBsaxpyOBrbc-xr3pn14WcbTt4OG1wTttw_TtjZyXNNbcs1Om162-EXdtxg6-n0cC_Yw-9ff1fX0d39n5vV1V2kVaLiKIEYCdIslQqwaDCPUadVklQUhkKQIkOVK6iFUjXUMktRU54Shsl1kelGLdi3vTckfpzIj2Vn_C4K9hRSlVIGSZFnSgX06zt0ayfXh3SBUoWIpUx21Pc9pZ313lFTDs506J5KAeWuhjLUUL7UENjzg3GqOqrfyNe9B-ByD_wzLT19bCpvVz_2ymeO1JEP</recordid><startdate>201908</startdate><enddate>201908</enddate><creator>Zhang, Jin</creator><creator>Liu, Shiyuan</creator><creator>Zhang, Danjie</creator><creator>Ma, Zhenchuan</creator><creator>Sun, Liangzhang</creator><general>Wiley Subscription Services, Inc</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7T7</scope><scope>7TK</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-0093-4121</orcidid></search><sort><creationdate>201908</creationdate><title>Homeobox D10, a tumor suppressor, inhibits the proliferation and migration of esophageal squamous cell carcinoma</title><author>Zhang, Jin ; Liu, Shiyuan ; Zhang, Danjie ; Ma, Zhenchuan ; Sun, Liangzhang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3534-504ae0676230a9fa84ac6b55be073a0217a3830d133d0d276ace86ea464c97cf3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>1-Phosphatidylinositol 3-kinase</topic><topic>AKT protein</topic><topic>Biotechnology</topic><topic>Cancer</topic><topic>Cell growth</topic><topic>Cell migration</topic><topic>Cell proliferation</topic><topic>Cholecystokinin</topic><topic>Ectopic expression</topic><topic>epithelial‐mesenchymal transition (EMT)</topic><topic>Esophageal cancer</topic><topic>esophageal squamous cell carcinoma (ESCC)</topic><topic>Esophagus</topic><topic>Growth factors</topic><topic>Homeobox</topic><topic>Homeobox D10 (HOXD10)</topic><topic>In vivo methods and tests</topic><topic>Insulin</topic><topic>invasion</topic><topic>Kinases</topic><topic>Mesenchyme</topic><topic>migration</topic><topic>PI3K/AKT/mTOR signaling pathway</topic><topic>Polymerase chain reaction</topic><topic>Proteins</topic><topic>Reverse transcription</topic><topic>Signal transduction</topic><topic>Signaling</topic><topic>Squamous cell carcinoma</topic><topic>TOR protein</topic><topic>Tumor suppressor genes</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Jin</creatorcontrib><creatorcontrib>Liu, Shiyuan</creatorcontrib><creatorcontrib>Zhang, Danjie</creatorcontrib><creatorcontrib>Ma, Zhenchuan</creatorcontrib><creatorcontrib>Sun, Liangzhang</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of cellular biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, Jin</au><au>Liu, Shiyuan</au><au>Zhang, Danjie</au><au>Ma, Zhenchuan</au><au>Sun, Liangzhang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Homeobox D10, a tumor suppressor, inhibits the proliferation and migration of esophageal squamous cell carcinoma</atitle><jtitle>Journal of cellular biochemistry</jtitle><addtitle>J Cell Biochem</addtitle><date>2019-08</date><risdate>2019</risdate><volume>120</volume><issue>8</issue><spage>13717</spage><epage>13725</epage><pages>13717-13725</pages><issn>0730-2312</issn><eissn>1097-4644</eissn><abstract>Esophageal squamous cell carcinoma (ESCC) is one of the most common types of esophageal cancer, which is the sixth leading cause of cancer death globally. Homeobox D10 (HOXD10) is a member of the homeobox (HOX) gene family and has been reported to act as a tumor suppressor. However, the potential role of HOXD10 in ESCC has not been reported. Thus, the aim of this study was to examine the expression and function of HOXD10 in ESCC. The expressions of HOXD10 in human ESCC tissues and cell lines were detected by quantitative reverse transcription polymerase chain reaction and Western blot. The HOXD10 overexpressing cell lines were established, then CCK‐8 and Transwell assays were performed to examine cell proliferation, migration, and invasion, respectively. The expression of EMT‐related proteins and signaling pathway‐related proteins were detected by Western blot. Our results showed that HOXD10 is lowly expressed in ESCC tissues as well as in ESCC cell lines. Ectopic overexpression of HOXD10 inhibited cell proliferation, migration, and invasion of ESCC cells (P < 0.05). HOXD10 overexpression repressed the epithelial‐mesenchymal transition (EMT) process in ESCC cells. Besides, HOXD10 overexpression suppressed the activation of PI3K/AKT/mTOR signaling pathway. PI3K/Akt agonist, insulin‐like growth factor‐1, reversed the inhibitory effects of HOXD10 on cell proliferation and migration in ESCC cells. Additional in vivo study proved that ectopic expression of HOXD10 caused an obvious inhibitory effect on the tumor growth. These findings indicated that overexpression of HOXD10 suppressed the proliferation, migration, and invasion via regulating the PI3K/AKT/mTOR signaling pathway in ESCC cells. Thus, targeting HOXD10 may be considered as a therapeutic strategy for ESCC treatment.
Overexpression of HOXD10 suppressed the proliferation, migration, and invasion via regulating the PI3K/AKT/mTOR signaling pathway in ESCC cells. Thus, targeting HOXD10 may be considered as a therapeutic strategy for ESCC treatment.</abstract><cop>United States</cop><pub>Wiley Subscription Services, Inc</pub><pmid>30938888</pmid><doi>10.1002/jcb.28644</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0003-0093-4121</orcidid></addata></record> |
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| subjects | 1-Phosphatidylinositol 3-kinase AKT protein Biotechnology Cancer Cell growth Cell migration Cell proliferation Cholecystokinin Ectopic expression epithelial‐mesenchymal transition (EMT) Esophageal cancer esophageal squamous cell carcinoma (ESCC) Esophagus Growth factors Homeobox Homeobox D10 (HOXD10) In vivo methods and tests Insulin invasion Kinases Mesenchyme migration PI3K/AKT/mTOR signaling pathway Polymerase chain reaction Proteins Reverse transcription Signal transduction Signaling Squamous cell carcinoma TOR protein Tumor suppressor genes Tumors |
| title | Homeobox D10, a tumor suppressor, inhibits the proliferation and migration of esophageal squamous cell carcinoma |
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