Room Temperature Detection of Plasma Epstein-Barr Virus DNA with CRISPR-Cas13

[...]quantitative PCR requires complex and expensive instrumentation, restricting its use in point-of-care testing and remote regions (4). [...]a simple, portable, and inexpensive EBV DNA detection assay is needed. According to a previous study (5), the SHERLOCK platform works at 37 °C. We optimized...

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Veröffentlicht in:	Clinical chemistry (Baltimore, Md.) Md.), 2019-04, Vol.65 (4), p.591-592
Hauptverfasser:	Wu, Yetao, Liu, Shang-Xin, Wang, Fang, Zeng, Mu-Sheng
Format:	Artikel
Sprache:	eng
Schlagworte:	Assaying Base Sequence Cancer Collaboration Correlation coefficient Correlation coefficients CRISPR CRISPR-Associated Proteins - genetics CRISPR-Cas Systems - genetics Deoxyribonucleic acid DNA DNA, Viral - blood Epstein-Barr virus Genetic testing Guanosine triphosphate Herpesvirus 4, Human - genetics Humans Instrumentation Laboratories Leptotrichia - chemistry Limit of Detection Magnesium chloride Nucleic Acid Amplification Techniques - methods Polymerase chain reaction Primers Rehydration Remote regions Ribonucleic acid RNA Substrates Temperature Temperature effects Viruses
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