Synthesis and biological evaluation of new bisindole-imidazopyridine hybrids as apoptosis inducers
[Display omitted] •Imidazo[1,5-a]pyridine-bisindole conjugates(5a-t) as apoptis inducedrs.•Conjugate 5k IC50 value 1.6 µM againt Lung cancer cell line.•Arrest cell cycle at G2/M phase. A series of diindolylmethanes (5a-t) were designed, synthesized, and examined for their cytotoxicity against four h...
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| Veröffentlicht in: | Bioorganic chemistry 2019-06, Vol.87, p.484-494 |
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| creator | Sunkari, Satish Bonam, Srinivasa Reddy Rao, A.V. Subba Riyaz, Sd Lakshma Nayak, V. Kumar, Halmuthur Mahabalarao Sampath Kamal, Ahmed Nagendra Babu, Bathini |
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•Imidazo[1,5-a]pyridine-bisindole conjugates(5a-t) as apoptis inducedrs.•Conjugate 5k IC50 value 1.6 µM againt Lung cancer cell line.•Arrest cell cycle at G2/M phase.
A series of diindolylmethanes (5a-t) were designed, synthesized, and examined for their cytotoxicity against four human cancer cell lines like prostate (DU-145), lung (A549), breast (MCF-7) and cervical cancer (HeLa). These results revealed that among all the hybrids, two (5k and 5r) were identified and exhibited significant cytotoxic effect against A549 cancer cells with IC50 values of 1.65 ± 0.3 and 1.80 ± 0.8 µM respectively. To investigate the reasons for the cytotoxic activity, the conventional biological assays were carried out with 5k and 5r on the A549 cancer cells. Both hybrids led to the arrest of A549 cell lines at the G2/M phase of the cell cycle and strongly induced apoptosis. Further the apoptotic effects of 5k and 5r were confirmed by ROS, annexin-V FITC, and mitochondrial membrane potential. Moreover, structure–activity relationships were elucidated with various substitutions on these hybrids. |
| doi_str_mv | 10.1016/j.bioorg.2019.03.061 |
| format | Article |
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•Imidazo[1,5-a]pyridine-bisindole conjugates(5a-t) as apoptis inducedrs.•Conjugate 5k IC50 value 1.6 µM againt Lung cancer cell line.•Arrest cell cycle at G2/M phase.
A series of diindolylmethanes (5a-t) were designed, synthesized, and examined for their cytotoxicity against four human cancer cell lines like prostate (DU-145), lung (A549), breast (MCF-7) and cervical cancer (HeLa). These results revealed that among all the hybrids, two (5k and 5r) were identified and exhibited significant cytotoxic effect against A549 cancer cells with IC50 values of 1.65 ± 0.3 and 1.80 ± 0.8 µM respectively. To investigate the reasons for the cytotoxic activity, the conventional biological assays were carried out with 5k and 5r on the A549 cancer cells. Both hybrids led to the arrest of A549 cell lines at the G2/M phase of the cell cycle and strongly induced apoptosis. Further the apoptotic effects of 5k and 5r were confirmed by ROS, annexin-V FITC, and mitochondrial membrane potential. Moreover, structure–activity relationships were elucidated with various substitutions on these hybrids.</description><identifier>ISSN: 0045-2068</identifier><identifier>EISSN: 1090-2120</identifier><identifier>DOI: 10.1016/j.bioorg.2019.03.061</identifier><identifier>PMID: 30927589</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Apoptosis ; Bisindole-imidazopyridine ; Cytotoxicity ; Heterocyclic ; ROS</subject><ispartof>Bioorganic chemistry, 2019-06, Vol.87, p.484-494</ispartof><rights>2019 Elsevier Inc.</rights><rights>Copyright © 2019 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c362t-43fbb524f5859886cbaf84977d26e329a9324ff05d27c57d790a778308b6668a3</citedby><cites>FETCH-LOGICAL-c362t-43fbb524f5859886cbaf84977d26e329a9324ff05d27c57d790a778308b6668a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.bioorg.2019.03.061$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3541,27915,27916,45986</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30927589$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sunkari, Satish</creatorcontrib><creatorcontrib>Bonam, Srinivasa Reddy</creatorcontrib><creatorcontrib>Rao, A.V. Subba</creatorcontrib><creatorcontrib>Riyaz, Sd</creatorcontrib><creatorcontrib>Lakshma Nayak, V.</creatorcontrib><creatorcontrib>Kumar, Halmuthur Mahabalarao Sampath</creatorcontrib><creatorcontrib>Kamal, Ahmed</creatorcontrib><creatorcontrib>Nagendra Babu, Bathini</creatorcontrib><title>Synthesis and biological evaluation of new bisindole-imidazopyridine hybrids as apoptosis inducers</title><title>Bioorganic chemistry</title><addtitle>Bioorg Chem</addtitle><description>[Display omitted]
•Imidazo[1,5-a]pyridine-bisindole conjugates(5a-t) as apoptis inducedrs.•Conjugate 5k IC50 value 1.6 µM againt Lung cancer cell line.•Arrest cell cycle at G2/M phase.
A series of diindolylmethanes (5a-t) were designed, synthesized, and examined for their cytotoxicity against four human cancer cell lines like prostate (DU-145), lung (A549), breast (MCF-7) and cervical cancer (HeLa). These results revealed that among all the hybrids, two (5k and 5r) were identified and exhibited significant cytotoxic effect against A549 cancer cells with IC50 values of 1.65 ± 0.3 and 1.80 ± 0.8 µM respectively. To investigate the reasons for the cytotoxic activity, the conventional biological assays were carried out with 5k and 5r on the A549 cancer cells. Both hybrids led to the arrest of A549 cell lines at the G2/M phase of the cell cycle and strongly induced apoptosis. Further the apoptotic effects of 5k and 5r were confirmed by ROS, annexin-V FITC, and mitochondrial membrane potential. Moreover, structure–activity relationships were elucidated with various substitutions on these hybrids.</description><subject>Apoptosis</subject><subject>Bisindole-imidazopyridine</subject><subject>Cytotoxicity</subject><subject>Heterocyclic</subject><subject>ROS</subject><issn>0045-2068</issn><issn>1090-2120</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNp9kE1r3DAQhkVJaTZp_0EpPuZiZyTZ-rgUQkjbQCCHtmchS-NEi9dyJTth--urZdMcAwMamGfeQQ8hnyk0FKi43DZ9iDE9NAyoboA3IOg7sqGgoWaUwQnZALRdzUCoU3KW8xaA0laKD-SUg2ayU3pD-p_7aXnEHHJlJ1-VyDE-BGfHCp_suNolxKmKQzXhcxnmMPk4Yh12wdu_cd6n4MOE1eO-L12JKDXHeYmHvMKuDlP-SN4Pdsz46eU9J7-_3fy6_lHf3X-_vb66qx0XbKlbPvR9x9qhU51WSrjeDqrVUnomkDNtNS_DATrPpOuklxqslIqD6oUQyvJzcnHMnVP8s2JezC5kh-NoJ4xrNowBSCELW9D2iLoUc044mDmFnU17Q8Ec7JqtOdo1B7sGuCl2y9qXlwtrv0P_uvRfZwG-HgEs_3wKmEx2ASeHPiR0i_ExvH3hH5Vzjn4</recordid><startdate>201906</startdate><enddate>201906</enddate><creator>Sunkari, Satish</creator><creator>Bonam, Srinivasa Reddy</creator><creator>Rao, A.V. Subba</creator><creator>Riyaz, Sd</creator><creator>Lakshma Nayak, V.</creator><creator>Kumar, Halmuthur Mahabalarao Sampath</creator><creator>Kamal, Ahmed</creator><creator>Nagendra Babu, Bathini</creator><general>Elsevier Inc</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201906</creationdate><title>Synthesis and biological evaluation of new bisindole-imidazopyridine hybrids as apoptosis inducers</title><author>Sunkari, Satish ; Bonam, Srinivasa Reddy ; Rao, A.V. Subba ; Riyaz, Sd ; Lakshma Nayak, V. ; Kumar, Halmuthur Mahabalarao Sampath ; Kamal, Ahmed ; Nagendra Babu, Bathini</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c362t-43fbb524f5859886cbaf84977d26e329a9324ff05d27c57d790a778308b6668a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Apoptosis</topic><topic>Bisindole-imidazopyridine</topic><topic>Cytotoxicity</topic><topic>Heterocyclic</topic><topic>ROS</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sunkari, Satish</creatorcontrib><creatorcontrib>Bonam, Srinivasa Reddy</creatorcontrib><creatorcontrib>Rao, A.V. Subba</creatorcontrib><creatorcontrib>Riyaz, Sd</creatorcontrib><creatorcontrib>Lakshma Nayak, V.</creatorcontrib><creatorcontrib>Kumar, Halmuthur Mahabalarao Sampath</creatorcontrib><creatorcontrib>Kamal, Ahmed</creatorcontrib><creatorcontrib>Nagendra Babu, Bathini</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Bioorganic chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sunkari, Satish</au><au>Bonam, Srinivasa Reddy</au><au>Rao, A.V. Subba</au><au>Riyaz, Sd</au><au>Lakshma Nayak, V.</au><au>Kumar, Halmuthur Mahabalarao Sampath</au><au>Kamal, Ahmed</au><au>Nagendra Babu, Bathini</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Synthesis and biological evaluation of new bisindole-imidazopyridine hybrids as apoptosis inducers</atitle><jtitle>Bioorganic chemistry</jtitle><addtitle>Bioorg Chem</addtitle><date>2019-06</date><risdate>2019</risdate><volume>87</volume><spage>484</spage><epage>494</epage><pages>484-494</pages><issn>0045-2068</issn><eissn>1090-2120</eissn><abstract>[Display omitted]
•Imidazo[1,5-a]pyridine-bisindole conjugates(5a-t) as apoptis inducedrs.•Conjugate 5k IC50 value 1.6 µM againt Lung cancer cell line.•Arrest cell cycle at G2/M phase.
A series of diindolylmethanes (5a-t) were designed, synthesized, and examined for their cytotoxicity against four human cancer cell lines like prostate (DU-145), lung (A549), breast (MCF-7) and cervical cancer (HeLa). These results revealed that among all the hybrids, two (5k and 5r) were identified and exhibited significant cytotoxic effect against A549 cancer cells with IC50 values of 1.65 ± 0.3 and 1.80 ± 0.8 µM respectively. To investigate the reasons for the cytotoxic activity, the conventional biological assays were carried out with 5k and 5r on the A549 cancer cells. Both hybrids led to the arrest of A549 cell lines at the G2/M phase of the cell cycle and strongly induced apoptosis. Further the apoptotic effects of 5k and 5r were confirmed by ROS, annexin-V FITC, and mitochondrial membrane potential. Moreover, structure–activity relationships were elucidated with various substitutions on these hybrids.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>30927589</pmid><doi>10.1016/j.bioorg.2019.03.061</doi><tpages>11</tpages></addata></record> |
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| subjects | Apoptosis Bisindole-imidazopyridine Cytotoxicity Heterocyclic ROS |
| title | Synthesis and biological evaluation of new bisindole-imidazopyridine hybrids as apoptosis inducers |
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