Colorimetric detection of glutathione based on its inhibitory effect on the peroxidase-mimicking properties of WS2 nanosheets
A highly sensitive colorimetric assay is described for the determination of glutathione (GSH). It is based on the use of tungsten disulfide (WS 2 ) which is a peroxidase mimic. It catalyzes the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) by hydrogen peroxide to form a blue-colored product (oxT...
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| Veröffentlicht in: | Mikrochimica acta (1966) 2019-04, Vol.186 (4), p.257-257, Article 257 |
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| container_title | Mikrochimica acta (1966) |
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| creator | Li, Li Wang, Qianna Chen, Zhengbo |
| description | A highly sensitive colorimetric assay is described for the determination of glutathione (GSH). It is based on the use of tungsten disulfide (WS
2
) which is a peroxidase mimic. It catalyzes the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) by hydrogen peroxide to form a blue-colored product (oxTMB) at near neutral pH values. In the presence of the analyte GSH, it is transformed to its oxidized form (GSSG), while the blue oxTMB is increasingly reduced and eventually is converted to colorless TMB. This can readily be detected with bare eyes. GSH can be determined spectroscopically (at 652 nm) by this method at concentrations down to 61 pM, and the response is linear in the 100 pM to 10 nM GSH concentration range. The assay is cost-effective and simple. Conceivably, it provides a promising tool for the determination of GSH in food and medical samples.
Graphical abstract
Schemaric of a sensitive method for colorimetric detection of glutathione (GSH) based on WS
2
-catalyzed oxidation of TMB by H
2
O
2
and the reduction of blue oxTMB by GSH. |
| doi_str_mv | 10.1007/s00604-019-3365-1 |
| format | Article |
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2
) which is a peroxidase mimic. It catalyzes the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) by hydrogen peroxide to form a blue-colored product (oxTMB) at near neutral pH values. In the presence of the analyte GSH, it is transformed to its oxidized form (GSSG), while the blue oxTMB is increasingly reduced and eventually is converted to colorless TMB. This can readily be detected with bare eyes. GSH can be determined spectroscopically (at 652 nm) by this method at concentrations down to 61 pM, and the response is linear in the 100 pM to 10 nM GSH concentration range. The assay is cost-effective and simple. Conceivably, it provides a promising tool for the determination of GSH in food and medical samples.
Graphical abstract
Schemaric of a sensitive method for colorimetric detection of glutathione (GSH) based on WS
2
-catalyzed oxidation of TMB by H
2
O
2
and the reduction of blue oxTMB by GSH.</description><identifier>ISSN: 0026-3672</identifier><identifier>EISSN: 1436-5073</identifier><identifier>DOI: 10.1007/s00604-019-3365-1</identifier><language>eng</language><publisher>Vienna: Springer Vienna</publisher><subject>Analytical Chemistry ; Characterization and Evaluation of Materials ; Chemistry ; Chemistry and Materials Science ; Microengineering ; Nanochemistry ; Nanotechnology ; Original Paper</subject><ispartof>Mikrochimica acta (1966), 2019-04, Vol.186 (4), p.257-257, Article 257</ispartof><rights>Springer-Verlag GmbH Austria, part of Springer Nature 2019</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c251t-4d4d390f3f53b3b8df53b21a026c291aef88d7546a08c0ffb63e28252ab4aa063</citedby><cites>FETCH-LOGICAL-c251t-4d4d390f3f53b3b8df53b21a026c291aef88d7546a08c0ffb63e28252ab4aa063</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00604-019-3365-1$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00604-019-3365-1$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids></links><search><creatorcontrib>Li, Li</creatorcontrib><creatorcontrib>Wang, Qianna</creatorcontrib><creatorcontrib>Chen, Zhengbo</creatorcontrib><title>Colorimetric detection of glutathione based on its inhibitory effect on the peroxidase-mimicking properties of WS2 nanosheets</title><title>Mikrochimica acta (1966)</title><addtitle>Microchim Acta</addtitle><description>A highly sensitive colorimetric assay is described for the determination of glutathione (GSH). It is based on the use of tungsten disulfide (WS
2
) which is a peroxidase mimic. It catalyzes the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) by hydrogen peroxide to form a blue-colored product (oxTMB) at near neutral pH values. In the presence of the analyte GSH, it is transformed to its oxidized form (GSSG), while the blue oxTMB is increasingly reduced and eventually is converted to colorless TMB. This can readily be detected with bare eyes. GSH can be determined spectroscopically (at 652 nm) by this method at concentrations down to 61 pM, and the response is linear in the 100 pM to 10 nM GSH concentration range. The assay is cost-effective and simple. Conceivably, it provides a promising tool for the determination of GSH in food and medical samples.
Graphical abstract
Schemaric of a sensitive method for colorimetric detection of glutathione (GSH) based on WS
2
-catalyzed oxidation of TMB by H
2
O
2
and the reduction of blue oxTMB by GSH.</description><subject>Analytical Chemistry</subject><subject>Characterization and Evaluation of Materials</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Microengineering</subject><subject>Nanochemistry</subject><subject>Nanotechnology</subject><subject>Original Paper</subject><issn>0026-3672</issn><issn>1436-5073</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNp9kD9PwzAQxS0EEqXwAdg8shjOduKkI6r4JyExAGK0nOTcuqRxsV2JDnx3HIWZ6enu3jvd_Qi55HDNAaqbCKCgYMAXTEpVMn5EZryQipVQyWMyAxCKSVWJU3IW4waAV0oUM_Kz9L0PbospuJZ2mLBNzg_UW7rq98mkda6QNiZiR3PfpUjdsHaNSz4cKFqbA-MgrZHuMPhv12Uv27qtaz_dsKK74HM_OYzj0o9XQQcz-LhGTPGcnFjTR7z40zl5v797Wz6y55eHp-XtM2tFyRMruqKTC7DSlrKRTd2NKrjJP7ViwQ3auu6qslAG6hasbZREUYtSmKYwBpSck6tpbz7ma48x6a2LLfa9GdDvoxZ8UZcKMrls5ZO1DT7GgFbvMh4TDpqDHlHrCbXOqPWIWvOcEVMmZu-wwqA3fh-G_NE_oV--e4Pb</recordid><startdate>20190401</startdate><enddate>20190401</enddate><creator>Li, Li</creator><creator>Wang, Qianna</creator><creator>Chen, Zhengbo</creator><general>Springer Vienna</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20190401</creationdate><title>Colorimetric detection of glutathione based on its inhibitory effect on the peroxidase-mimicking properties of WS2 nanosheets</title><author>Li, Li ; Wang, Qianna ; Chen, Zhengbo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c251t-4d4d390f3f53b3b8df53b21a026c291aef88d7546a08c0ffb63e28252ab4aa063</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Analytical Chemistry</topic><topic>Characterization and Evaluation of Materials</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Microengineering</topic><topic>Nanochemistry</topic><topic>Nanotechnology</topic><topic>Original Paper</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Li</creatorcontrib><creatorcontrib>Wang, Qianna</creatorcontrib><creatorcontrib>Chen, Zhengbo</creatorcontrib><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Mikrochimica acta (1966)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Li</au><au>Wang, Qianna</au><au>Chen, Zhengbo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Colorimetric detection of glutathione based on its inhibitory effect on the peroxidase-mimicking properties of WS2 nanosheets</atitle><jtitle>Mikrochimica acta (1966)</jtitle><stitle>Microchim Acta</stitle><date>2019-04-01</date><risdate>2019</risdate><volume>186</volume><issue>4</issue><spage>257</spage><epage>257</epage><pages>257-257</pages><artnum>257</artnum><issn>0026-3672</issn><eissn>1436-5073</eissn><abstract>A highly sensitive colorimetric assay is described for the determination of glutathione (GSH). It is based on the use of tungsten disulfide (WS
2
) which is a peroxidase mimic. It catalyzes the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) by hydrogen peroxide to form a blue-colored product (oxTMB) at near neutral pH values. In the presence of the analyte GSH, it is transformed to its oxidized form (GSSG), while the blue oxTMB is increasingly reduced and eventually is converted to colorless TMB. This can readily be detected with bare eyes. GSH can be determined spectroscopically (at 652 nm) by this method at concentrations down to 61 pM, and the response is linear in the 100 pM to 10 nM GSH concentration range. The assay is cost-effective and simple. Conceivably, it provides a promising tool for the determination of GSH in food and medical samples.
Graphical abstract
Schemaric of a sensitive method for colorimetric detection of glutathione (GSH) based on WS
2
-catalyzed oxidation of TMB by H
2
O
2
and the reduction of blue oxTMB by GSH.</abstract><cop>Vienna</cop><pub>Springer Vienna</pub><doi>10.1007/s00604-019-3365-1</doi><tpages>1</tpages></addata></record> |
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| language | eng |
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| source | SpringerLink Journals - AutoHoldings |
| subjects | Analytical Chemistry Characterization and Evaluation of Materials Chemistry Chemistry and Materials Science Microengineering Nanochemistry Nanotechnology Original Paper |
| title | Colorimetric detection of glutathione based on its inhibitory effect on the peroxidase-mimicking properties of WS2 nanosheets |
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