Insights into the ubiquinol/dioxygen binding and proton relay pathways of the alternative oxidase
The alternative oxidase (AOX) is a monotopic diiron carboxylate protein which catalyzes the four-electron reduction of dioxygen to water by ubiquinol. Although we have recently determined the crystal structure of Trypanosoma brucei AOX (TAO) in the presence and absence of ascofuranone (AF) derivativ...
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| Veröffentlicht in: | Biochimica et biophysica acta. Bioenergetics 2019-05, Vol.1860 (5), p.375-382 |
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| creator | Shiba, Tomoo Inaoka, Daniel Ken Takahashi, Gen Tsuge, Chiaki Kido, Yasutoshi Young, Luke Ueda, Satoshi Balogun, Emmanuel Oluwadare Nara, Takeshi Honma, Teruki Tanaka, Akiko Inoue, Masayuki Saimoto, Hiroyuki Harada, Shigeharu Moore, Anthony L. Kita, Kiyoshi |
| description | The alternative oxidase (AOX) is a monotopic diiron carboxylate protein which catalyzes the four-electron reduction of dioxygen to water by ubiquinol. Although we have recently determined the crystal structure of Trypanosoma brucei AOX (TAO) in the presence and absence of ascofuranone (AF) derivatives (which are potent mixed type inhibitors) the mechanism by which ubiquinol and dioxygen binds to TAO remain inconclusive. In this article, ferulenol was identified as the first competitive inhibitor of AOX which has been used to probe the binding of ubiquinol. Surface plasmon resonance reveals that AF is a quasi-irreversible inhibitor of TAO whilst ferulenol binding is completely reversible. The structure of the TAO-ferulenol complex, determined at 2.7 Å, provided insights into ubiquinol binding and has also identified a potential dioxygen molecule bound in a side-on conformation to the diiron center for the first time. |
| doi_str_mv | 10.1016/j.bbabio.2019.03.008 |
| format | Article |
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| source | Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; ScienceDirect Journals (5 years ago - present) |
| subjects | Alternative oxidase Competitive inhibitor Ferulenol Proton relay pathway Trypanosoma brucei Ubiquinol/dioxygen binding |
| title | Insights into the ubiquinol/dioxygen binding and proton relay pathways of the alternative oxidase |
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