Genetically Encoded Biotin Analogues: Incorporation and Application in Bacterial and Mammalian Cells

The biotin–streptavidin interaction is among the strongest known in nature. Herein, the site‐directed incorporation of biotin and 2‐iminobiotin composed of noncanonical amino acids (ncAAs) into proteins is reported. 2‐Iminobiotin lysine was employed for protein purification based on the pH‐dependent...

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Veröffentlicht in:	Chembiochem : a European journal of chemical biology 2019-07, Vol.20 (14), p.1795-1798
Hauptverfasser:	Hohl, Adrian, Mideksa, Yonatan G., Karan, Ram, Akal, Anastassja, Vogler, Malvina, Groll, Michael, Rueping, Magnus, Lang, Kathrin, Feige, Matthias J., Eppinger, Jörg
Format:	Artikel
Sprache:	eng
Schlagworte:	Affinity Amino acids Biotin Biotinylation Genetic code Immobilization Lysine Mammalian cells Mammals mutagenesis noncovalent interactions Protein purification Proteins RecA protein Streptavidin
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