Diversity of the Fusarium pathogens associated with crown rot in the Huanghuai wheat‐growing region of China
Summary To investigate the distribution and diversity of the pathogens associated with Fusarium crown rot in the Huanghuai wheat‐growing region (HHWGR) of China, we collected wheat samples with symptomatic stem bases from seven provinces in the HHWGR between 2013 and 2016. A total of 1196 isolates o...
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| Veröffentlicht in: | Environmental microbiology 2019-08, Vol.21 (8), p.2740-2754 |
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| creator | Zhou, Haifeng He, Xiaolun Wang, Shuo Ma, Qingzhou Sun, Bingjian Ding, Shengli Chen, Linlin Zhang, Meng Li, Honglian |
| description | Summary
To investigate the distribution and diversity of the pathogens associated with Fusarium crown rot in the Huanghuai wheat‐growing region (HHWGR) of China, we collected wheat samples with symptomatic stem bases from seven provinces in the HHWGR between 2013 and 2016. A total of 1196 isolates obtained from 222 locations were identified as 9 Fusarium species based on morphological and molecular identification. Of these pathogen species, F. pseudograminearum was the dominant species. Furthermore, F. sinensis was isolated from the disease specimens and tested for virulence to wheat. The result of the pathogenicity revealed that an intraspecific differentiation existed in F. pseudograminearum; sequence analysis of the EF‐1α gene showed that 194 F. pseudograminearum isolates were differentiated into two distinct clades which closed to the strains from Australia and China respectively, but neither pathogenicity nor EF‐1α sequence was related to the geographic origins of these isolates. However, universal rice primers‐polymerase chain reaction showed a correlation with the geographical origins of the 194 isolates, which were divided into eight subclusters, the level of genetic diversity was higher within a geographical population than among the different populations. The results of these analyses can be directly used to facilitate disease monitoring and development of control strategies. |
| doi_str_mv | 10.1111/1462-2920.14602 |
| format | Article |
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To investigate the distribution and diversity of the pathogens associated with Fusarium crown rot in the Huanghuai wheat‐growing region (HHWGR) of China, we collected wheat samples with symptomatic stem bases from seven provinces in the HHWGR between 2013 and 2016. A total of 1196 isolates obtained from 222 locations were identified as 9 Fusarium species based on morphological and molecular identification. Of these pathogen species, F. pseudograminearum was the dominant species. Furthermore, F. sinensis was isolated from the disease specimens and tested for virulence to wheat. The result of the pathogenicity revealed that an intraspecific differentiation existed in F. pseudograminearum; sequence analysis of the EF‐1α gene showed that 194 F. pseudograminearum isolates were differentiated into two distinct clades which closed to the strains from Australia and China respectively, but neither pathogenicity nor EF‐1α sequence was related to the geographic origins of these isolates. However, universal rice primers‐polymerase chain reaction showed a correlation with the geographical origins of the 194 isolates, which were divided into eight subclusters, the level of genetic diversity was higher within a geographical population than among the different populations. The results of these analyses can be directly used to facilitate disease monitoring and development of control strategies.</description><identifier>ISSN: 1462-2912</identifier><identifier>EISSN: 1462-2920</identifier><identifier>DOI: 10.1111/1462-2920.14602</identifier><identifier>PMID: 30897256</identifier><language>eng</language><publisher>Hoboken, USA: John Wiley & Sons, Inc</publisher><subject>China ; Crown rot ; Disease control ; Dominant species ; Fusarium ; Fusarium - genetics ; Genetic diversity ; Genetic Variation ; Geographical distribution ; Nucleotide sequence ; Origins ; Pathogenicity ; Pathogens ; PCR ; Plant Diseases - microbiology ; Polymerase Chain Reaction ; Primers ; Sequencing ; Triticum - microbiology ; Virulence ; Wheat</subject><ispartof>Environmental microbiology, 2019-08, Vol.21 (8), p.2740-2754</ispartof><rights>2019 Society for Applied Microbiology and John Wiley & Sons Ltd.</rights><rights>2019 Society for Applied Microbiology and John Wiley & Sons Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3712-be405237a1d57822322c97d994cdf10060b7b359ba0d630154f446988a2d95053</citedby><cites>FETCH-LOGICAL-c3712-be405237a1d57822322c97d994cdf10060b7b359ba0d630154f446988a2d95053</cites><orcidid>0000-0002-0985-506X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2F1462-2920.14602$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2F1462-2920.14602$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30897256$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhou, Haifeng</creatorcontrib><creatorcontrib>He, Xiaolun</creatorcontrib><creatorcontrib>Wang, Shuo</creatorcontrib><creatorcontrib>Ma, Qingzhou</creatorcontrib><creatorcontrib>Sun, Bingjian</creatorcontrib><creatorcontrib>Ding, Shengli</creatorcontrib><creatorcontrib>Chen, Linlin</creatorcontrib><creatorcontrib>Zhang, Meng</creatorcontrib><creatorcontrib>Li, Honglian</creatorcontrib><title>Diversity of the Fusarium pathogens associated with crown rot in the Huanghuai wheat‐growing region of China</title><title>Environmental microbiology</title><addtitle>Environ Microbiol</addtitle><description>Summary
To investigate the distribution and diversity of the pathogens associated with Fusarium crown rot in the Huanghuai wheat‐growing region (HHWGR) of China, we collected wheat samples with symptomatic stem bases from seven provinces in the HHWGR between 2013 and 2016. A total of 1196 isolates obtained from 222 locations were identified as 9 Fusarium species based on morphological and molecular identification. Of these pathogen species, F. pseudograminearum was the dominant species. Furthermore, F. sinensis was isolated from the disease specimens and tested for virulence to wheat. The result of the pathogenicity revealed that an intraspecific differentiation existed in F. pseudograminearum; sequence analysis of the EF‐1α gene showed that 194 F. pseudograminearum isolates were differentiated into two distinct clades which closed to the strains from Australia and China respectively, but neither pathogenicity nor EF‐1α sequence was related to the geographic origins of these isolates. However, universal rice primers‐polymerase chain reaction showed a correlation with the geographical origins of the 194 isolates, which were divided into eight subclusters, the level of genetic diversity was higher within a geographical population than among the different populations. The results of these analyses can be directly used to facilitate disease monitoring and development of control strategies.</description><subject>China</subject><subject>Crown rot</subject><subject>Disease control</subject><subject>Dominant species</subject><subject>Fusarium</subject><subject>Fusarium - genetics</subject><subject>Genetic diversity</subject><subject>Genetic Variation</subject><subject>Geographical distribution</subject><subject>Nucleotide sequence</subject><subject>Origins</subject><subject>Pathogenicity</subject><subject>Pathogens</subject><subject>PCR</subject><subject>Plant Diseases - microbiology</subject><subject>Polymerase Chain Reaction</subject><subject>Primers</subject><subject>Sequencing</subject><subject>Triticum - microbiology</subject><subject>Virulence</subject><subject>Wheat</subject><issn>1462-2912</issn><issn>1462-2920</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkbtOwzAUhi0E4j6zIUssLAX7JLbrEZWrBGKB2XISJzFq7WInVN14BJ6RJ8G90IEFL77oO5-O_4PQCSUXNK1LmnMYgIR0zTmBLbS_ednenCnsoYMY3wihIhNkF-1lZCgFML6P3LX9MCHabo59jbvW4Ns-6mD7CZ7qrvWNcRHrGH1pdWcqPLNdi8vgZw4H32HrljX3vXZN22uLZ63R3ffnV5MQ6xocTGO9W7hHrXX6CO3UehzN8Xo_RK-3Ny-j-8Hj893D6OpxUGaCwqAwOWGQCU0rJoYAGUApRSVlXlY1JYSTQhQZk4UmFc8IZXmd51wOhxoqyQjLDtH5yjsN_r03sVMTG0szHmtnfB8VUMkZMEZ5Qs_-oG--Dy51pwC44ERAThJ1uaLS32MMplbTYCc6zBUlajEKtQhbLYJXy1GkitO1ty8mptrwv9kngK2AmR2b-X8-dfP0sBL_AHk9kmU</recordid><startdate>201908</startdate><enddate>201908</enddate><creator>Zhou, Haifeng</creator><creator>He, Xiaolun</creator><creator>Wang, Shuo</creator><creator>Ma, Qingzhou</creator><creator>Sun, Bingjian</creator><creator>Ding, Shengli</creator><creator>Chen, Linlin</creator><creator>Zhang, Meng</creator><creator>Li, Honglian</creator><general>John Wiley & Sons, Inc</general><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QH</scope><scope>7QL</scope><scope>7ST</scope><scope>7T7</scope><scope>7TN</scope><scope>7U9</scope><scope>7UA</scope><scope>8FD</scope><scope>C1K</scope><scope>F1W</scope><scope>FR3</scope><scope>H94</scope><scope>H95</scope><scope>H97</scope><scope>L.G</scope><scope>M7N</scope><scope>P64</scope><scope>SOI</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-0985-506X</orcidid></search><sort><creationdate>201908</creationdate><title>Diversity of the Fusarium pathogens associated with crown rot in the Huanghuai wheat‐growing region of China</title><author>Zhou, Haifeng ; He, Xiaolun ; Wang, Shuo ; Ma, Qingzhou ; Sun, Bingjian ; Ding, Shengli ; Chen, Linlin ; Zhang, Meng ; Li, Honglian</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3712-be405237a1d57822322c97d994cdf10060b7b359ba0d630154f446988a2d95053</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>China</topic><topic>Crown rot</topic><topic>Disease control</topic><topic>Dominant species</topic><topic>Fusarium</topic><topic>Fusarium - genetics</topic><topic>Genetic diversity</topic><topic>Genetic Variation</topic><topic>Geographical distribution</topic><topic>Nucleotide sequence</topic><topic>Origins</topic><topic>Pathogenicity</topic><topic>Pathogens</topic><topic>PCR</topic><topic>Plant Diseases - microbiology</topic><topic>Polymerase Chain Reaction</topic><topic>Primers</topic><topic>Sequencing</topic><topic>Triticum - microbiology</topic><topic>Virulence</topic><topic>Wheat</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhou, Haifeng</creatorcontrib><creatorcontrib>He, Xiaolun</creatorcontrib><creatorcontrib>Wang, Shuo</creatorcontrib><creatorcontrib>Ma, Qingzhou</creatorcontrib><creatorcontrib>Sun, Bingjian</creatorcontrib><creatorcontrib>Ding, Shengli</creatorcontrib><creatorcontrib>Chen, Linlin</creatorcontrib><creatorcontrib>Zhang, Meng</creatorcontrib><creatorcontrib>Li, Honglian</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Aqualine</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environment Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Oceanic Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Water Resources Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 3: Aquatic Pollution & Environmental Quality</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environment Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Environmental microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhou, Haifeng</au><au>He, Xiaolun</au><au>Wang, Shuo</au><au>Ma, Qingzhou</au><au>Sun, Bingjian</au><au>Ding, Shengli</au><au>Chen, Linlin</au><au>Zhang, Meng</au><au>Li, Honglian</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Diversity of the Fusarium pathogens associated with crown rot in the Huanghuai wheat‐growing region of China</atitle><jtitle>Environmental microbiology</jtitle><addtitle>Environ Microbiol</addtitle><date>2019-08</date><risdate>2019</risdate><volume>21</volume><issue>8</issue><spage>2740</spage><epage>2754</epage><pages>2740-2754</pages><issn>1462-2912</issn><eissn>1462-2920</eissn><abstract>Summary
To investigate the distribution and diversity of the pathogens associated with Fusarium crown rot in the Huanghuai wheat‐growing region (HHWGR) of China, we collected wheat samples with symptomatic stem bases from seven provinces in the HHWGR between 2013 and 2016. A total of 1196 isolates obtained from 222 locations were identified as 9 Fusarium species based on morphological and molecular identification. Of these pathogen species, F. pseudograminearum was the dominant species. Furthermore, F. sinensis was isolated from the disease specimens and tested for virulence to wheat. The result of the pathogenicity revealed that an intraspecific differentiation existed in F. pseudograminearum; sequence analysis of the EF‐1α gene showed that 194 F. pseudograminearum isolates were differentiated into two distinct clades which closed to the strains from Australia and China respectively, but neither pathogenicity nor EF‐1α sequence was related to the geographic origins of these isolates. However, universal rice primers‐polymerase chain reaction showed a correlation with the geographical origins of the 194 isolates, which were divided into eight subclusters, the level of genetic diversity was higher within a geographical population than among the different populations. The results of these analyses can be directly used to facilitate disease monitoring and development of control strategies.</abstract><cop>Hoboken, USA</cop><pub>John Wiley & Sons, Inc</pub><pmid>30897256</pmid><doi>10.1111/1462-2920.14602</doi><tpages>15</tpages><orcidid>https://orcid.org/0000-0002-0985-506X</orcidid></addata></record> |
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| subjects | China Crown rot Disease control Dominant species Fusarium Fusarium - genetics Genetic diversity Genetic Variation Geographical distribution Nucleotide sequence Origins Pathogenicity Pathogens PCR Plant Diseases - microbiology Polymerase Chain Reaction Primers Sequencing Triticum - microbiology Virulence Wheat |
| title | Diversity of the Fusarium pathogens associated with crown rot in the Huanghuai wheat‐growing region of China |
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