A multi-analyte LC–MS/MS method for screening and quantification of 16 synthetic cathinones in hair: Application to postmortem cases

[Display omitted] •A method for the determination of 16 synthetic cathinones in hair.•Though a low amount of sample, a high sensitivity was achieved.•2 out of 17 postmortem samples provided positive results for 9 different cathinones. A multi-analyte method for detection and quantification of 16 syn...

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Veröffentlicht in:Forensic science international 2019-05, Vol.298, p.115-120
Hauptverfasser: Freni, Francesca, Bianco, Sara, Vignali, Claudia, Groppi, Angelo, Moretti, Matteo, Osculati, Antonio Marco Maria, Morini, Luca
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container_title Forensic science international
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creator Freni, Francesca
Bianco, Sara
Vignali, Claudia
Groppi, Angelo
Moretti, Matteo
Osculati, Antonio Marco Maria
Morini, Luca
description [Display omitted] •A method for the determination of 16 synthetic cathinones in hair.•Though a low amount of sample, a high sensitivity was achieved.•2 out of 17 postmortem samples provided positive results for 9 different cathinones. A multi-analyte method for detection and quantification of 16 synthetic cathinones (known also as “bath salts”) in human hair has been developed and fully validated using liquid chromatography–tandem mass spectrometry system. About 20 mg of hair samples, previously washed and homogenized, were ultrasonicated with 1 mL HCl 0.1 M solution. Samples were then extracted using a solid phase extraction procedure (SPE), taken to dryness and reconstituted in 100 μL mobile phase. Finally, they were directly injected into a liquid chromatographic system, coupled with tandem mass spectrometer detector. The validation criteria parameters were satisfactory according with the international guidelines. A LOQ of 5 pg/mg was obtained for 4-fluoromethcathinone (4-FMC), buphedrone, ethcathinone, methcathinone, mephedrone and naphyrone, while the method proved to be more sensitive for 4-methylethcathinone (4-MEC), methedrone, alpha-pyrrolidinopentiophenone (α-PVP), alpha-pyrrolidinohexiophenone (α-PHP), methylenedioxypyrovalerone (MDPV), butylone, ethylone, 3,4-dimethylmethcathinone (3,4—DMMC), pentedrone and pentylone, reaching a LOQ of 1 pg/mg. Potential use of bath salts was investigated in postmortem cases of young subjects previously tested positive at least to one traditional drug of abuse. Two samples out of 17 cases analyzed provided positive results for synthetic cathinones. One sample has been divided in two segments of 2.5 cm length each. Both segments were positive for 8 different cathinone derivatives, namely: 3,4-DMMC, 4-FMC, 4-MEC, α-PHP, α-PVP, methcathinone, methedrone and pentedrone. The second case provided positive results for ethcathinone.
doi_str_mv 10.1016/j.forsciint.2019.02.036
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A multi-analyte method for detection and quantification of 16 synthetic cathinones (known also as “bath salts”) in human hair has been developed and fully validated using liquid chromatography–tandem mass spectrometry system. About 20 mg of hair samples, previously washed and homogenized, were ultrasonicated with 1 mL HCl 0.1 M solution. Samples were then extracted using a solid phase extraction procedure (SPE), taken to dryness and reconstituted in 100 μL mobile phase. Finally, they were directly injected into a liquid chromatographic system, coupled with tandem mass spectrometer detector. The validation criteria parameters were satisfactory according with the international guidelines. 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A multi-analyte method for detection and quantification of 16 synthetic cathinones (known also as “bath salts”) in human hair has been developed and fully validated using liquid chromatography–tandem mass spectrometry system. About 20 mg of hair samples, previously washed and homogenized, were ultrasonicated with 1 mL HCl 0.1 M solution. Samples were then extracted using a solid phase extraction procedure (SPE), taken to dryness and reconstituted in 100 μL mobile phase. Finally, they were directly injected into a liquid chromatographic system, coupled with tandem mass spectrometer detector. The validation criteria parameters were satisfactory according with the international guidelines. 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subjects Accuracy
Calibration
Chromatography
Cocaine
Forensic sciences
Hair
Identification
LC–MS/MS
Medical screening
Methadone
Nitrogen
Postmortem cases
Synthetic cathinones
Urine
title A multi-analyte LC–MS/MS method for screening and quantification of 16 synthetic cathinones in hair: Application to postmortem cases
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