Genetic toolkits of the red alga Pyropia tenera against the three most common diseases in Pyropia farms
Disease outbreaks devastate Pyropia aquaculture farms every year. The three most common and serious diseases are Olpidiopsis‐blight and red‐rot disease caused by oomycete pathogens and green‐spot disease caused by the PyroV1 virus. We hypothesized that a basic genetic profile of molecular defenses w...
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| Veröffentlicht in: | Journal of phycology 2019-08, Vol.55 (4), p.801-815 |
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| creator | Im, Soo Hyun Klochkova, Tatyana A. Lee, Da Jeoung Gachon, Claire M. M. Kim, Gwang Hoon Buschmann, A. |
| description | Disease outbreaks devastate Pyropia aquaculture farms every year. The three most common and serious diseases are Olpidiopsis‐blight and red‐rot disease caused by oomycete pathogens and green‐spot disease caused by the PyroV1 virus. We hypothesized that a basic genetic profile of molecular defenses will be revealed by comparing and analyzing the genetic response of Pyropia tenera against the above three pathogens. RNAs isolated from infected thalli were hybridized onto an oligochip containing 15,115 primers designed from P. tenera expressed sequence tags (EST)s. Microarray profiles of the three diseases were compared and interpreted together with histochemical observation. Massive amounts of reactive oxygen species accumulated in P. tenera cells exposed to oomycete pathogens. Heat shock genes and serine proteases were the most highly up‐regulated genes in all infection experiments. Genes involved in RNA metabolism, ribosomal proteins and antioxidant metabolism were also highly up‐regulated. Genetic profiles of P. tenera in response to pathogens were most similar between the two biotrophic pathogens, Olpidiopsis pyropiae and PyroV1 virus. A group of plant resistance genes were specifically regulated against each pathogen. Our results suggested that disease response in P. tenera consists of a general constitutive defense and a genetic toolkit against specific pathogens. |
| doi_str_mv | 10.1111/jpy.12857 |
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M. ; Kim, Gwang Hoon ; Buschmann, A.</creator><contributor>Buschmann, A.</contributor><creatorcontrib>Im, Soo Hyun ; Klochkova, Tatyana A. ; Lee, Da Jeoung ; Gachon, Claire M. M. ; Kim, Gwang Hoon ; Buschmann, A. ; Buschmann, A.</creatorcontrib><description>Disease outbreaks devastate Pyropia aquaculture farms every year. The three most common and serious diseases are Olpidiopsis‐blight and red‐rot disease caused by oomycete pathogens and green‐spot disease caused by the PyroV1 virus. We hypothesized that a basic genetic profile of molecular defenses will be revealed by comparing and analyzing the genetic response of Pyropia tenera against the above three pathogens. RNAs isolated from infected thalli were hybridized onto an oligochip containing 15,115 primers designed from P. tenera expressed sequence tags (EST)s. Microarray profiles of the three diseases were compared and interpreted together with histochemical observation. Massive amounts of reactive oxygen species accumulated in P. tenera cells exposed to oomycete pathogens. Heat shock genes and serine proteases were the most highly up‐regulated genes in all infection experiments. Genes involved in RNA metabolism, ribosomal proteins and antioxidant metabolism were also highly up‐regulated. Genetic profiles of P. tenera in response to pathogens were most similar between the two biotrophic pathogens, Olpidiopsis pyropiae and PyroV1 virus. A group of plant resistance genes were specifically regulated against each pathogen. Our results suggested that disease response in P. tenera consists of a general constitutive defense and a genetic toolkit against specific pathogens.</description><identifier>ISSN: 0022-3646</identifier><identifier>EISSN: 1529-8817</identifier><identifier>DOI: 10.1111/jpy.12857</identifier><identifier>PMID: 30897208</identifier><language>eng</language><publisher>United States: Wiley Subscription Services, Inc</publisher><subject>Antioxidants ; Aquaculture ; Blight ; disease ; Disease outbreaks ; Disease resistance ; Expressed sequence tags ; Farms ; Fish diseases ; Genes ; Genes, Plant ; Heat shock ; innate immunity ; Metabolism ; microarray ; Nucleic acids ; Nucleotide sequence ; Olpidiopsis ; Oomycete ; Outbreaks ; Pathogens ; Plant resistance ; Plant viruses ; Primers ; Profiles ; Pyropia ; Pyropia tenera ; Reactive oxygen species ; Rhodophyta ; Ribonucleic acid ; Ribosomal proteins ; RNA ; Rot ; rRNA ; Serine ; Thalli ; Toolkits ; virus ; Viruses</subject><ispartof>Journal of phycology, 2019-08, Vol.55 (4), p.801-815</ispartof><rights>2019 Phycological Society of America</rights><rights>2019 Phycological Society of America.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4197-dd014e90c6755f9b88a771a1f3165001fee5797b31846316794055792326e5cb3</citedby><cites>FETCH-LOGICAL-c4197-dd014e90c6755f9b88a771a1f3165001fee5797b31846316794055792326e5cb3</cites><orcidid>0000-0002-0009-8277</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fjpy.12857$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fjpy.12857$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30897208$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Buschmann, A.</contributor><creatorcontrib>Im, Soo Hyun</creatorcontrib><creatorcontrib>Klochkova, Tatyana A.</creatorcontrib><creatorcontrib>Lee, Da Jeoung</creatorcontrib><creatorcontrib>Gachon, Claire M. M.</creatorcontrib><creatorcontrib>Kim, Gwang Hoon</creatorcontrib><creatorcontrib>Buschmann, A.</creatorcontrib><title>Genetic toolkits of the red alga Pyropia tenera against the three most common diseases in Pyropia farms</title><title>Journal of phycology</title><addtitle>J Phycol</addtitle><description>Disease outbreaks devastate Pyropia aquaculture farms every year. The three most common and serious diseases are Olpidiopsis‐blight and red‐rot disease caused by oomycete pathogens and green‐spot disease caused by the PyroV1 virus. We hypothesized that a basic genetic profile of molecular defenses will be revealed by comparing and analyzing the genetic response of Pyropia tenera against the above three pathogens. RNAs isolated from infected thalli were hybridized onto an oligochip containing 15,115 primers designed from P. tenera expressed sequence tags (EST)s. Microarray profiles of the three diseases were compared and interpreted together with histochemical observation. Massive amounts of reactive oxygen species accumulated in P. tenera cells exposed to oomycete pathogens. Heat shock genes and serine proteases were the most highly up‐regulated genes in all infection experiments. Genes involved in RNA metabolism, ribosomal proteins and antioxidant metabolism were also highly up‐regulated. Genetic profiles of P. tenera in response to pathogens were most similar between the two biotrophic pathogens, Olpidiopsis pyropiae and PyroV1 virus. A group of plant resistance genes were specifically regulated against each pathogen. Our results suggested that disease response in P. tenera consists of a general constitutive defense and a genetic toolkit against specific pathogens.</description><subject>Antioxidants</subject><subject>Aquaculture</subject><subject>Blight</subject><subject>disease</subject><subject>Disease outbreaks</subject><subject>Disease resistance</subject><subject>Expressed sequence tags</subject><subject>Farms</subject><subject>Fish diseases</subject><subject>Genes</subject><subject>Genes, Plant</subject><subject>Heat shock</subject><subject>innate immunity</subject><subject>Metabolism</subject><subject>microarray</subject><subject>Nucleic acids</subject><subject>Nucleotide sequence</subject><subject>Olpidiopsis</subject><subject>Oomycete</subject><subject>Outbreaks</subject><subject>Pathogens</subject><subject>Plant resistance</subject><subject>Plant viruses</subject><subject>Primers</subject><subject>Profiles</subject><subject>Pyropia</subject><subject>Pyropia tenera</subject><subject>Reactive oxygen species</subject><subject>Rhodophyta</subject><subject>Ribonucleic acid</subject><subject>Ribosomal proteins</subject><subject>RNA</subject><subject>Rot</subject><subject>rRNA</subject><subject>Serine</subject><subject>Thalli</subject><subject>Toolkits</subject><subject>virus</subject><subject>Viruses</subject><issn>0022-3646</issn><issn>1529-8817</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp10EFLwzAUB_Agis7pwS8gAS966JaXNk16lKFTGehBD55C1r7OzLaZSYfs2xudehDMJeTxy5_Hn5ATYCOIZ7xcbUbAlZA7ZACCF4lSIHfJgDHOkzTP8gNyGMKSMSZzAfvkIGWqkJypAVlMscPelrR3rnm1faCupv0LUo8VNc3C0IeNdytraB-hN9QsjO1C_2X6F49IWxefpWtb19HKBjQBA7Xd78fa-DYckb3aNAGPv-8hebq-epzcJLP76e3kcpaUGRQyqSoGGRaszKUQdTFXykgJBuoUcsEY1IhCFnKegsryOJNFxkSc8JTnKMp5OiTn29yVd29rDL1ubSixaUyHbh00hyIXPM2ARXr2hy7d2ndxO8255KA4V1lUF1tVeheCx1qvvG2N32hg-rN9HdvXX-1He_qduJ63WP3Kn7ojGG_Bu21w83-Svnt43kZ-ALEvjM4</recordid><startdate>201908</startdate><enddate>201908</enddate><creator>Im, Soo Hyun</creator><creator>Klochkova, Tatyana A.</creator><creator>Lee, Da Jeoung</creator><creator>Gachon, Claire M. M.</creator><creator>Kim, Gwang Hoon</creator><creator>Buschmann, A.</creator><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TN</scope><scope>F1W</scope><scope>H95</scope><scope>L.G</scope><scope>M7N</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-0009-8277</orcidid></search><sort><creationdate>201908</creationdate><title>Genetic toolkits of the red alga Pyropia tenera against the three most common diseases in Pyropia farms</title><author>Im, Soo Hyun ; Klochkova, Tatyana A. ; Lee, Da Jeoung ; Gachon, Claire M. M. ; Kim, Gwang Hoon ; Buschmann, A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4197-dd014e90c6755f9b88a771a1f3165001fee5797b31846316794055792326e5cb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Antioxidants</topic><topic>Aquaculture</topic><topic>Blight</topic><topic>disease</topic><topic>Disease outbreaks</topic><topic>Disease resistance</topic><topic>Expressed sequence tags</topic><topic>Farms</topic><topic>Fish diseases</topic><topic>Genes</topic><topic>Genes, Plant</topic><topic>Heat shock</topic><topic>innate immunity</topic><topic>Metabolism</topic><topic>microarray</topic><topic>Nucleic acids</topic><topic>Nucleotide sequence</topic><topic>Olpidiopsis</topic><topic>Oomycete</topic><topic>Outbreaks</topic><topic>Pathogens</topic><topic>Plant resistance</topic><topic>Plant viruses</topic><topic>Primers</topic><topic>Profiles</topic><topic>Pyropia</topic><topic>Pyropia tenera</topic><topic>Reactive oxygen species</topic><topic>Rhodophyta</topic><topic>Ribonucleic acid</topic><topic>Ribosomal proteins</topic><topic>RNA</topic><topic>Rot</topic><topic>rRNA</topic><topic>Serine</topic><topic>Thalli</topic><topic>Toolkits</topic><topic>virus</topic><topic>Viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Im, Soo Hyun</creatorcontrib><creatorcontrib>Klochkova, Tatyana A.</creatorcontrib><creatorcontrib>Lee, Da Jeoung</creatorcontrib><creatorcontrib>Gachon, Claire M. M.</creatorcontrib><creatorcontrib>Kim, Gwang Hoon</creatorcontrib><creatorcontrib>Buschmann, A.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Oceanic Abstracts</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of phycology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Im, Soo Hyun</au><au>Klochkova, Tatyana A.</au><au>Lee, Da Jeoung</au><au>Gachon, Claire M. M.</au><au>Kim, Gwang Hoon</au><au>Buschmann, A.</au><au>Buschmann, A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genetic toolkits of the red alga Pyropia tenera against the three most common diseases in Pyropia farms</atitle><jtitle>Journal of phycology</jtitle><addtitle>J Phycol</addtitle><date>2019-08</date><risdate>2019</risdate><volume>55</volume><issue>4</issue><spage>801</spage><epage>815</epage><pages>801-815</pages><issn>0022-3646</issn><eissn>1529-8817</eissn><abstract>Disease outbreaks devastate Pyropia aquaculture farms every year. The three most common and serious diseases are Olpidiopsis‐blight and red‐rot disease caused by oomycete pathogens and green‐spot disease caused by the PyroV1 virus. We hypothesized that a basic genetic profile of molecular defenses will be revealed by comparing and analyzing the genetic response of Pyropia tenera against the above three pathogens. RNAs isolated from infected thalli were hybridized onto an oligochip containing 15,115 primers designed from P. tenera expressed sequence tags (EST)s. Microarray profiles of the three diseases were compared and interpreted together with histochemical observation. Massive amounts of reactive oxygen species accumulated in P. tenera cells exposed to oomycete pathogens. Heat shock genes and serine proteases were the most highly up‐regulated genes in all infection experiments. Genes involved in RNA metabolism, ribosomal proteins and antioxidant metabolism were also highly up‐regulated. Genetic profiles of P. tenera in response to pathogens were most similar between the two biotrophic pathogens, Olpidiopsis pyropiae and PyroV1 virus. A group of plant resistance genes were specifically regulated against each pathogen. Our results suggested that disease response in P. tenera consists of a general constitutive defense and a genetic toolkit against specific pathogens.</abstract><cop>United States</cop><pub>Wiley Subscription Services, Inc</pub><pmid>30897208</pmid><doi>10.1111/jpy.12857</doi><tpages>15</tpages><orcidid>https://orcid.org/0000-0002-0009-8277</orcidid></addata></record> |
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| subjects | Antioxidants Aquaculture Blight disease Disease outbreaks Disease resistance Expressed sequence tags Farms Fish diseases Genes Genes, Plant Heat shock innate immunity Metabolism microarray Nucleic acids Nucleotide sequence Olpidiopsis Oomycete Outbreaks Pathogens Plant resistance Plant viruses Primers Profiles Pyropia Pyropia tenera Reactive oxygen species Rhodophyta Ribonucleic acid Ribosomal proteins RNA Rot rRNA Serine Thalli Toolkits virus Viruses |
| title | Genetic toolkits of the red alga Pyropia tenera against the three most common diseases in Pyropia farms |
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