Differential analysis of very small particles (VSP) from the contact surfaces and recessed areas of footwear

•Particles on contact surfaces of soles are attributable to the most recent exposure.•Particles in recessed areas of soles contain particles from prior exposures.•New methods of particle combination analysis can measure degrees of mixture.•Differential analysis can be applied to specimens from contact and recessed areas.•Computational methods allow detection and deconvolution of particle mixtures. Prior work has shown that the contact surfaces of footwear rapidly lose very small particles (VSP) when walking on dry soil. Other research, with more general sampling from shoe soles, has shown that particles can persist much longer. Given rapid losses from contact surfaces, we hypothesize that non-contact, recessed areas of footwear retain particles from prior exposures. Here we conduct differential analysis of VSP recovered separately from contact and recessed surfaces, following a controlled series of environmental exposures. Work boots and tennis shoes were exposed by walking distances of 250 m, sequentially, in three environmental sites. VSP were harvested separately from contact and recessed surfaces using a moist swabbing procedure. VSP were analyzed by microscopy and the proportions attributable to each site were determined using a newly developed statistical model. The principal findings are (1) contact surfaces of footwear are dominated by VSP attributable to the most recent site of exposure, (2) recessed surfaces of footwear retain VSP from prior exposures, (3) sole type appears to be a source of major differences in the amount of VSP from prior exposures remaining in recessed areas, and (4) when VSP attributable to prior exposures are found, there is no clear trend for dominance between earlier exposures. The significance of these findings is considerable. In cases where the last site visited is of interest, VSP from contact surfaces of footwear will give a nearly pure sample of that site. In cases where prior sites visited are of interest, the VSP from the contact surfaces can be used as a background signal, providing a means for differential analysis of VSP mixtures found in the recessed areas of the sole. The strong influence of shoe sole type on the retention of VSP from prior exposures indicates that efforts to explore the rates of VSP loss from recessed areas will need to take characteristics of the sole type into account. The absence of a clear trend for dominance between earlier exposures suggests that once traces are lodged within the recess