Diacylglycerol kinase and associated lipid mediators modulate rice root architecture
Diacylglycerol kinase (DGK) phosphorylates diacylglycerol (DAG) to generate phosphatidic acid (PA), and both DAG and PA are lipid mediators in the cell. Here we show that DGK1 in rice (Oryza sativa) plays important roles in root growth and development. Two independent OsDGK1-knockout (dgk1) lines ex...
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| Veröffentlicht in: | The New phytologist 2019-07, Vol.223 (1), p.261-276 |
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| creator | Yuan, Shu Kim, Sang-Chul Deng, Xianjun Hong, Yueyun Wang, Xuemin |
| description | Diacylglycerol kinase (DGK) phosphorylates diacylglycerol (DAG) to generate phosphatidic acid (PA), and both DAG and PA are lipid mediators in the cell. Here we show that DGK1 in rice (Oryza sativa) plays important roles in root growth and development.
Two independent OsDGK1-knockout (dgk1) lines exhibited a higher density of lateral roots (LRs) and thinner seminal roots (SRs), whereas OsDGK1-overexpressing plants displayed a lower LR density and thicker SRs than wild-type (WT) plants.
Overexpression of OsDGK1 led to a decline in the DGK substrate DAG whereas specific PA species decreased in dgk1 roots. Supplementation of DAG to OsDGK1-overexpressing seedlings restored the LR density and SR thickness whereas application of PA to dgk1 seedlings restored the LR density and SR thickness to those of the WT. In addition, treatment of rice seedlings with the DGK inhibitor R59022 increased the level of DAG and decreased PA, which also restored the root phenotype of OsDGK1-overexpressing seedlings close to that of the WT.
Together, these results indicate that DGK1 and associated lipid mediators modulate rice root architecture; DAG promotes LR formation and suppresses SR growth whereas PA suppresses LR number and promotes SR thickness. |
| doi_str_mv | 10.1111/nph.15801 |
| format | Article |
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Two independent OsDGK1-knockout (dgk1) lines exhibited a higher density of lateral roots (LRs) and thinner seminal roots (SRs), whereas OsDGK1-overexpressing plants displayed a lower LR density and thicker SRs than wild-type (WT) plants.
Overexpression of OsDGK1 led to a decline in the DGK substrate DAG whereas specific PA species decreased in dgk1 roots. Supplementation of DAG to OsDGK1-overexpressing seedlings restored the LR density and SR thickness whereas application of PA to dgk1 seedlings restored the LR density and SR thickness to those of the WT. In addition, treatment of rice seedlings with the DGK inhibitor R59022 increased the level of DAG and decreased PA, which also restored the root phenotype of OsDGK1-overexpressing seedlings close to that of the WT.
Together, these results indicate that DGK1 and associated lipid mediators modulate rice root architecture; DAG promotes LR formation and suppresses SR growth whereas PA suppresses LR number and promotes SR thickness.</description><identifier>ISSN: 0028-646X</identifier><identifier>EISSN: 1469-8137</identifier><identifier>DOI: 10.1111/nph.15801</identifier><identifier>PMID: 30887532</identifier><language>eng</language><publisher>England: Wiley</publisher><subject>Aquatic plants ; Architecture ; diacylglycerol ; Diacylglycerol kinase ; Diacylglycerol Kinase - genetics ; Diacylglycerol Kinase - metabolism ; diacylglycerols ; Diglycerides ; Diglycerides - metabolism ; Gene Expression Regulation, Plant ; Homozygote ; Kinases ; Lipid Metabolism ; lipid signaling ; Lipids ; Models, Biological ; Mutation - genetics ; Oryza - enzymology ; Oryza - genetics ; Oryza sativa ; phenotype ; Phenotypes ; Phosphatidic acid ; Phosphatidic Acids - metabolism ; Plant growth ; Plant Roots - anatomy & histology ; Plant Roots - enzymology ; Plant Roots - genetics ; Plant Roots - growth & development ; Plants, Genetically Modified ; Rice ; root architecture ; root growth ; Roots ; Seedlings ; Substrates ; Supplements ; Thickness ; Transcriptome - genetics</subject><ispartof>The New phytologist, 2019-07, Vol.223 (1), p.261-276</ispartof><rights>2019 The Authors © 2019 New Phytologist Trust</rights><rights>2019 The Authors. New Phytologist © 2019 New Phytologist Trust</rights><rights>2019 The Authors. New Phytologist © 2019 New Phytologist Trust.</rights><rights>Copyright © 2019 New Phytologist Trust</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4831-cb91986a402aa1361def5df87bb1d1c72045d0be546331dcbe02ac3d0c8db5113</citedby><cites>FETCH-LOGICAL-c4831-cb91986a402aa1361def5df87bb1d1c72045d0be546331dcbe02ac3d0c8db5113</cites><orcidid>0000-0002-7185-966X ; 0000-0003-3145-7727 ; 0000-0002-6251-6745 ; 0000-0002-0301-3750</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/26675979$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/26675979$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,776,780,799,1411,1427,27903,27904,45553,45554,46387,46811,57995,58228</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30887532$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yuan, Shu</creatorcontrib><creatorcontrib>Kim, Sang-Chul</creatorcontrib><creatorcontrib>Deng, Xianjun</creatorcontrib><creatorcontrib>Hong, Yueyun</creatorcontrib><creatorcontrib>Wang, Xuemin</creatorcontrib><title>Diacylglycerol kinase and associated lipid mediators modulate rice root architecture</title><title>The New phytologist</title><addtitle>New Phytol</addtitle><description>Diacylglycerol kinase (DGK) phosphorylates diacylglycerol (DAG) to generate phosphatidic acid (PA), and both DAG and PA are lipid mediators in the cell. Here we show that DGK1 in rice (Oryza sativa) plays important roles in root growth and development.
Two independent OsDGK1-knockout (dgk1) lines exhibited a higher density of lateral roots (LRs) and thinner seminal roots (SRs), whereas OsDGK1-overexpressing plants displayed a lower LR density and thicker SRs than wild-type (WT) plants.
Overexpression of OsDGK1 led to a decline in the DGK substrate DAG whereas specific PA species decreased in dgk1 roots. Supplementation of DAG to OsDGK1-overexpressing seedlings restored the LR density and SR thickness whereas application of PA to dgk1 seedlings restored the LR density and SR thickness to those of the WT. In addition, treatment of rice seedlings with the DGK inhibitor R59022 increased the level of DAG and decreased PA, which also restored the root phenotype of OsDGK1-overexpressing seedlings close to that of the WT.
Together, these results indicate that DGK1 and associated lipid mediators modulate rice root architecture; DAG promotes LR formation and suppresses SR growth whereas PA suppresses LR number and promotes SR thickness.</description><subject>Aquatic plants</subject><subject>Architecture</subject><subject>diacylglycerol</subject><subject>Diacylglycerol kinase</subject><subject>Diacylglycerol Kinase - genetics</subject><subject>Diacylglycerol Kinase - metabolism</subject><subject>diacylglycerols</subject><subject>Diglycerides</subject><subject>Diglycerides - metabolism</subject><subject>Gene Expression Regulation, Plant</subject><subject>Homozygote</subject><subject>Kinases</subject><subject>Lipid Metabolism</subject><subject>lipid signaling</subject><subject>Lipids</subject><subject>Models, Biological</subject><subject>Mutation - genetics</subject><subject>Oryza - enzymology</subject><subject>Oryza - genetics</subject><subject>Oryza sativa</subject><subject>phenotype</subject><subject>Phenotypes</subject><subject>Phosphatidic acid</subject><subject>Phosphatidic Acids - metabolism</subject><subject>Plant growth</subject><subject>Plant Roots - anatomy & histology</subject><subject>Plant Roots - enzymology</subject><subject>Plant Roots - genetics</subject><subject>Plant Roots - growth & development</subject><subject>Plants, Genetically Modified</subject><subject>Rice</subject><subject>root architecture</subject><subject>root growth</subject><subject>Roots</subject><subject>Seedlings</subject><subject>Substrates</subject><subject>Supplements</subject><subject>Thickness</subject><subject>Transcriptome - genetics</subject><issn>0028-646X</issn><issn>1469-8137</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1LBSEUhiWKun0s-gHFQJtaTHl0dJxl9A1RLQraiaPe8uYdbzpD3H-fdatFELk4Iuc5DxxfhLYBH0I-R93s-RCYwLCERlDxphRA62U0wpiIklf8cQ2tpzTBGDeMk1W0RrEQNaNkhO5PndJz_-Tn2sbgixfXqWQL1ZlCpRS0U701hXczZ4qpNfkZYiqmwQw-d4rodC4h9IWK-tn1VvdDtJtoZax8sltf9wZ6OD-7P7ksr28vrk6Or0tdCQqlbhtoBFcVJkoB5WDsmJmxqNsWDOia4IoZ3FpWcUrB6NZmUFODtTAtA6AbaH_hncXwOtjUy6lL2nqvOhuGJAnFDHgNhP2PQlNBRTn5sO79QidhiF1eRBJCadbVDGfqYEHpGFKKdixn0U1VnEvA8iMVmVORn6lkdvfLOLT5E3_I7xgycLQA3py3879N8ubu8lu5s5iYpJzIzwThvGZN3dB3rXWftw</recordid><startdate>20190701</startdate><enddate>20190701</enddate><creator>Yuan, Shu</creator><creator>Kim, Sang-Chul</creator><creator>Deng, Xianjun</creator><creator>Hong, Yueyun</creator><creator>Wang, Xuemin</creator><general>Wiley</general><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7SN</scope><scope>8FD</scope><scope>C1K</scope><scope>F1W</scope><scope>FR3</scope><scope>H95</scope><scope>L.G</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope><orcidid>https://orcid.org/0000-0002-7185-966X</orcidid><orcidid>https://orcid.org/0000-0003-3145-7727</orcidid><orcidid>https://orcid.org/0000-0002-6251-6745</orcidid><orcidid>https://orcid.org/0000-0002-0301-3750</orcidid></search><sort><creationdate>20190701</creationdate><title>Diacylglycerol kinase and associated lipid mediators modulate rice root architecture</title><author>Yuan, Shu ; Kim, Sang-Chul ; Deng, Xianjun ; Hong, Yueyun ; Wang, Xuemin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4831-cb91986a402aa1361def5df87bb1d1c72045d0be546331dcbe02ac3d0c8db5113</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Aquatic plants</topic><topic>Architecture</topic><topic>diacylglycerol</topic><topic>Diacylglycerol kinase</topic><topic>Diacylglycerol Kinase - genetics</topic><topic>Diacylglycerol Kinase - metabolism</topic><topic>diacylglycerols</topic><topic>Diglycerides</topic><topic>Diglycerides - metabolism</topic><topic>Gene Expression Regulation, Plant</topic><topic>Homozygote</topic><topic>Kinases</topic><topic>Lipid Metabolism</topic><topic>lipid signaling</topic><topic>Lipids</topic><topic>Models, Biological</topic><topic>Mutation - genetics</topic><topic>Oryza - enzymology</topic><topic>Oryza - genetics</topic><topic>Oryza sativa</topic><topic>phenotype</topic><topic>Phenotypes</topic><topic>Phosphatidic acid</topic><topic>Phosphatidic Acids - metabolism</topic><topic>Plant growth</topic><topic>Plant Roots - anatomy & histology</topic><topic>Plant Roots - enzymology</topic><topic>Plant Roots - genetics</topic><topic>Plant Roots - growth & development</topic><topic>Plants, Genetically Modified</topic><topic>Rice</topic><topic>root architecture</topic><topic>root growth</topic><topic>Roots</topic><topic>Seedlings</topic><topic>Substrates</topic><topic>Supplements</topic><topic>Thickness</topic><topic>Transcriptome - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yuan, Shu</creatorcontrib><creatorcontrib>Kim, Sang-Chul</creatorcontrib><creatorcontrib>Deng, Xianjun</creatorcontrib><creatorcontrib>Hong, Yueyun</creatorcontrib><creatorcontrib>Wang, Xuemin</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Ecology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>The New phytologist</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yuan, Shu</au><au>Kim, Sang-Chul</au><au>Deng, Xianjun</au><au>Hong, Yueyun</au><au>Wang, Xuemin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Diacylglycerol kinase and associated lipid mediators modulate rice root architecture</atitle><jtitle>The New phytologist</jtitle><addtitle>New Phytol</addtitle><date>2019-07-01</date><risdate>2019</risdate><volume>223</volume><issue>1</issue><spage>261</spage><epage>276</epage><pages>261-276</pages><issn>0028-646X</issn><eissn>1469-8137</eissn><abstract>Diacylglycerol kinase (DGK) phosphorylates diacylglycerol (DAG) to generate phosphatidic acid (PA), and both DAG and PA are lipid mediators in the cell. Here we show that DGK1 in rice (Oryza sativa) plays important roles in root growth and development.
Two independent OsDGK1-knockout (dgk1) lines exhibited a higher density of lateral roots (LRs) and thinner seminal roots (SRs), whereas OsDGK1-overexpressing plants displayed a lower LR density and thicker SRs than wild-type (WT) plants.
Overexpression of OsDGK1 led to a decline in the DGK substrate DAG whereas specific PA species decreased in dgk1 roots. Supplementation of DAG to OsDGK1-overexpressing seedlings restored the LR density and SR thickness whereas application of PA to dgk1 seedlings restored the LR density and SR thickness to those of the WT. In addition, treatment of rice seedlings with the DGK inhibitor R59022 increased the level of DAG and decreased PA, which also restored the root phenotype of OsDGK1-overexpressing seedlings close to that of the WT.
Together, these results indicate that DGK1 and associated lipid mediators modulate rice root architecture; DAG promotes LR formation and suppresses SR growth whereas PA suppresses LR number and promotes SR thickness.</abstract><cop>England</cop><pub>Wiley</pub><pmid>30887532</pmid><doi>10.1111/nph.15801</doi><tpages>16</tpages><orcidid>https://orcid.org/0000-0002-7185-966X</orcidid><orcidid>https://orcid.org/0000-0003-3145-7727</orcidid><orcidid>https://orcid.org/0000-0002-6251-6745</orcidid><orcidid>https://orcid.org/0000-0002-0301-3750</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Aquatic plants Architecture diacylglycerol Diacylglycerol kinase Diacylglycerol Kinase - genetics Diacylglycerol Kinase - metabolism diacylglycerols Diglycerides Diglycerides - metabolism Gene Expression Regulation, Plant Homozygote Kinases Lipid Metabolism lipid signaling Lipids Models, Biological Mutation - genetics Oryza - enzymology Oryza - genetics Oryza sativa phenotype Phenotypes Phosphatidic acid Phosphatidic Acids - metabolism Plant growth Plant Roots - anatomy & histology Plant Roots - enzymology Plant Roots - genetics Plant Roots - growth & development Plants, Genetically Modified Rice root architecture root growth Roots Seedlings Substrates Supplements Thickness Transcriptome - genetics |
| title | Diacylglycerol kinase and associated lipid mediators modulate rice root architecture |
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