Lysosome-targeted photoactivation reveals local sphingosine metabolism signatures
Lipids are essential components of eukaryotic cell membranes and play crucial roles in cellular signaling and metabolism. While increasing evidence shows that the activities of lipids are dependent upon subcellular localization, tools to study local lipid metabolism and signaling are limited. Herein...
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| Veröffentlicht in: | Chemical science (Cambridge) 2019-02, Vol.10 (8), p.2253-2258 |
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| container_title | Chemical science (Cambridge) |
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| creator | Feng, Suihan Harayama, Takeshi Chang, Dalu Hannich, J Thomas Winssinger, Nicolas Riezman, Howard |
| description | Lipids are essential components of eukaryotic cell membranes and play crucial roles in cellular signaling and metabolism. While increasing evidence shows that the activities of lipids are dependent upon subcellular localization, tools to study local lipid metabolism and signaling are limited. Herein, we report an approach that enabled us to selectively deliver photo-caged lipids into lysosomes and thereafter to quickly release the lipid molecules by illumination. On combining this method with genetic techniques and lipidomics, we were able to investigate the localization-dependent metabolism of an important intermediate of sphingolipid metabolism, sphingosine. Our data reveal a distinct metabolic pattern of lysosomal sphingosine. In general, this method has the potential to serve as a platform to study lysosomal metabolism and signaling of various lipids and metabolites in living cells. |
| doi_str_mv | 10.1039/c8sc03614d |
| format | Article |
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| source | DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central Open Access; PubMed Central |
| subjects | Cell membranes Lipid metabolism Lipids Localization Lysosomes Metabolism Metabolites Signaling |
| title | Lysosome-targeted photoactivation reveals local sphingosine metabolism signatures |
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