The effects of different sampling techniques on peripheral post mortem tryptase levels: a recommended sampling method
Different sampling techniques can impact on post mortem tryptase levels. A previous study demonstrated significantly lower femoral post mortem total tryptase levels in samples collected via transcutaneous aspiration compared with directly sampling during internal examination. However, an outlier wit...
Gespeichert in:
| Veröffentlicht in: | International journal of legal medicine 2019-09, Vol.133 (5), p.1477-1483 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1483 |
|---|---|
| container_issue | 5 |
| container_start_page | 1477 |
| container_title | International journal of legal medicine |
| container_volume | 133 |
| creator | Garland, J. Philcox, W. McCarthy, S. Hensby-Bennet, S. Ondruschka, B. Woydt, L. Da Broi, U. Palmiere, C. Lam, L. Ahn, Y. Kesha, K. Stables, S. Tse, R. |
| description | Different sampling techniques can impact on post mortem tryptase levels. A previous study demonstrated significantly lower femoral post mortem total tryptase levels in samples collected via transcutaneous aspiration compared with directly sampling during internal examination. However, an outlier with high tryptase level was noted in one transcutaneous aspiration sample. This 6-month prospective study compared total post mortem tryptase levels between 21 paired aspirated venous and arterial femoral blood samples, and 19 paired aspirated and cutdown femoral venous blood samples in non-anaphylactic deaths only. No statistical differences were demonstrated between the different sampling methods. However, four outlier cases with higher tryptase levels in aspirated arterial and femoral cutdown samples compared with aspirated venous femoral samples were noted. The reasons for the outliers may be due to the bloods collected from these two methods being contaminated by central arterial and venous blood with high tryptase levels respectively. None of the aspirated venous femoral post mortem tryptase levels were above recognized post mortem tryptase cutoff to diagnose anaphylaxis. This study recommends aspirating blood samples from a clamped femoral/external iliac vein for post mortem tryptase analysis should be defined as the gold standard. Further study using the recommended sampling method on post mortem tryptase levels in non-anaphylactic and anaphylactic cases is warranted. |
| doi_str_mv | 10.1007/s00414-019-02038-9 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2193605206</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2191941786</sourcerecordid><originalsourceid>FETCH-LOGICAL-c375t-45206f5592dc00d31f6584fafa3fb6af76ec796219cc3ca08478201984bfce43</originalsourceid><addsrcrecordid>eNp9kU9P3DAQxa0KVLbQL9ADssSll5Rx7MRxbwj1n4TUy94trzNmg-I4tR0kvj0OC0XiwMkj-ffePM0j5AuDbwxAXiYAwUQFTFVQA-8q9YFsmOCyYo1qj8gGVJlVV8sT8imlOwAmW9l8JCccOqkY5xuybPdI0Tm0OdHgaD-UOeKUaTJ-Hofplma0-2n4t2ABJjpjHOY9RjPSOaRMfYgZPc3xYc4mIR3xHsf0nRoa0QbvceqxfzXzmPehPyPHzowJPz-_p2T788f2-nd18_fXn-urm8py2eRKNDW0rmlU3VuAnjPXNp1wxhnudq1xskUrVVszZS23Bjohu7qcoxM7Z1HwU_L1YDvHsObP2g_J4jiaCcOSdBHyFtYlBb14g96FJU4l3EoxJZjsVqo-UDaGlCI6PcfBm_igGei1E33oRJcQ-qkTrYro_Nl62Xns_0teSigAPwCpfE23GF93v2P7CHO5mGo</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2191941786</pqid></control><display><type>article</type><title>The effects of different sampling techniques on peripheral post mortem tryptase levels: a recommended sampling method</title><source>MEDLINE</source><source>HeinOnline Law Journal Library</source><source>SpringerLink Journals - AutoHoldings</source><creator>Garland, J. ; Philcox, W. ; McCarthy, S. ; Hensby-Bennet, S. ; Ondruschka, B. ; Woydt, L. ; Da Broi, U. ; Palmiere, C. ; Lam, L. ; Ahn, Y. ; Kesha, K. ; Stables, S. ; Tse, R.</creator><creatorcontrib>Garland, J. ; Philcox, W. ; McCarthy, S. ; Hensby-Bennet, S. ; Ondruschka, B. ; Woydt, L. ; Da Broi, U. ; Palmiere, C. ; Lam, L. ; Ahn, Y. ; Kesha, K. ; Stables, S. ; Tse, R.</creatorcontrib><description>Different sampling techniques can impact on post mortem tryptase levels. A previous study demonstrated significantly lower femoral post mortem total tryptase levels in samples collected via transcutaneous aspiration compared with directly sampling during internal examination. However, an outlier with high tryptase level was noted in one transcutaneous aspiration sample. This 6-month prospective study compared total post mortem tryptase levels between 21 paired aspirated venous and arterial femoral blood samples, and 19 paired aspirated and cutdown femoral venous blood samples in non-anaphylactic deaths only. No statistical differences were demonstrated between the different sampling methods. However, four outlier cases with higher tryptase levels in aspirated arterial and femoral cutdown samples compared with aspirated venous femoral samples were noted. The reasons for the outliers may be due to the bloods collected from these two methods being contaminated by central arterial and venous blood with high tryptase levels respectively. None of the aspirated venous femoral post mortem tryptase levels were above recognized post mortem tryptase cutoff to diagnose anaphylaxis. This study recommends aspirating blood samples from a clamped femoral/external iliac vein for post mortem tryptase analysis should be defined as the gold standard. Further study using the recommended sampling method on post mortem tryptase levels in non-anaphylactic and anaphylactic cases is warranted.</description><identifier>ISSN: 0937-9827</identifier><identifier>EISSN: 1437-1596</identifier><identifier>DOI: 10.1007/s00414-019-02038-9</identifier><identifier>PMID: 30879133</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Adult ; Aged ; Aged, 80 and over ; Anaphylaxis ; Autopsy - methods ; Blood ; Blood Specimen Collection - methods ; Constriction ; Female ; Femoral Artery ; Femoral Vein ; Forensic Medicine ; Forensic pathology ; Forensic Pathology - methods ; Humans ; Male ; Medical Law ; Medicine & Public Health ; Method Paper ; Middle Aged ; Outliers (statistics) ; Prospective Studies ; Sampling methods ; Sampling techniques ; Statistical methods ; Tryptases - blood ; Young Adult</subject><ispartof>International journal of legal medicine, 2019-09, Vol.133 (5), p.1477-1483</ispartof><rights>Springer-Verlag GmbH Germany, part of Springer Nature 2019</rights><rights>International Journal of Legal Medicine is a copyright of Springer, (2019). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c375t-45206f5592dc00d31f6584fafa3fb6af76ec796219cc3ca08478201984bfce43</citedby><cites>FETCH-LOGICAL-c375t-45206f5592dc00d31f6584fafa3fb6af76ec796219cc3ca08478201984bfce43</cites><orcidid>0000-0002-1388-9537</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00414-019-02038-9$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00414-019-02038-9$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30879133$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Garland, J.</creatorcontrib><creatorcontrib>Philcox, W.</creatorcontrib><creatorcontrib>McCarthy, S.</creatorcontrib><creatorcontrib>Hensby-Bennet, S.</creatorcontrib><creatorcontrib>Ondruschka, B.</creatorcontrib><creatorcontrib>Woydt, L.</creatorcontrib><creatorcontrib>Da Broi, U.</creatorcontrib><creatorcontrib>Palmiere, C.</creatorcontrib><creatorcontrib>Lam, L.</creatorcontrib><creatorcontrib>Ahn, Y.</creatorcontrib><creatorcontrib>Kesha, K.</creatorcontrib><creatorcontrib>Stables, S.</creatorcontrib><creatorcontrib>Tse, R.</creatorcontrib><title>The effects of different sampling techniques on peripheral post mortem tryptase levels: a recommended sampling method</title><title>International journal of legal medicine</title><addtitle>Int J Legal Med</addtitle><addtitle>Int J Legal Med</addtitle><description>Different sampling techniques can impact on post mortem tryptase levels. A previous study demonstrated significantly lower femoral post mortem total tryptase levels in samples collected via transcutaneous aspiration compared with directly sampling during internal examination. However, an outlier with high tryptase level was noted in one transcutaneous aspiration sample. This 6-month prospective study compared total post mortem tryptase levels between 21 paired aspirated venous and arterial femoral blood samples, and 19 paired aspirated and cutdown femoral venous blood samples in non-anaphylactic deaths only. No statistical differences were demonstrated between the different sampling methods. However, four outlier cases with higher tryptase levels in aspirated arterial and femoral cutdown samples compared with aspirated venous femoral samples were noted. The reasons for the outliers may be due to the bloods collected from these two methods being contaminated by central arterial and venous blood with high tryptase levels respectively. None of the aspirated venous femoral post mortem tryptase levels were above recognized post mortem tryptase cutoff to diagnose anaphylaxis. This study recommends aspirating blood samples from a clamped femoral/external iliac vein for post mortem tryptase analysis should be defined as the gold standard. Further study using the recommended sampling method on post mortem tryptase levels in non-anaphylactic and anaphylactic cases is warranted.</description><subject>Adult</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Anaphylaxis</subject><subject>Autopsy - methods</subject><subject>Blood</subject><subject>Blood Specimen Collection - methods</subject><subject>Constriction</subject><subject>Female</subject><subject>Femoral Artery</subject><subject>Femoral Vein</subject><subject>Forensic Medicine</subject><subject>Forensic pathology</subject><subject>Forensic Pathology - methods</subject><subject>Humans</subject><subject>Male</subject><subject>Medical Law</subject><subject>Medicine & Public Health</subject><subject>Method Paper</subject><subject>Middle Aged</subject><subject>Outliers (statistics)</subject><subject>Prospective Studies</subject><subject>Sampling methods</subject><subject>Sampling techniques</subject><subject>Statistical methods</subject><subject>Tryptases - blood</subject><subject>Young Adult</subject><issn>0937-9827</issn><issn>1437-1596</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp9kU9P3DAQxa0KVLbQL9ADssSll5Rx7MRxbwj1n4TUy94trzNmg-I4tR0kvj0OC0XiwMkj-ffePM0j5AuDbwxAXiYAwUQFTFVQA-8q9YFsmOCyYo1qj8gGVJlVV8sT8imlOwAmW9l8JCccOqkY5xuybPdI0Tm0OdHgaD-UOeKUaTJ-Hofplma0-2n4t2ABJjpjHOY9RjPSOaRMfYgZPc3xYc4mIR3xHsf0nRoa0QbvceqxfzXzmPehPyPHzowJPz-_p2T788f2-nd18_fXn-urm8py2eRKNDW0rmlU3VuAnjPXNp1wxhnudq1xskUrVVszZS23Bjohu7qcoxM7Z1HwU_L1YDvHsObP2g_J4jiaCcOSdBHyFtYlBb14g96FJU4l3EoxJZjsVqo-UDaGlCI6PcfBm_igGei1E33oRJcQ-qkTrYro_Nl62Xns_0teSigAPwCpfE23GF93v2P7CHO5mGo</recordid><startdate>20190901</startdate><enddate>20190901</enddate><creator>Garland, J.</creator><creator>Philcox, W.</creator><creator>McCarthy, S.</creator><creator>Hensby-Bennet, S.</creator><creator>Ondruschka, B.</creator><creator>Woydt, L.</creator><creator>Da Broi, U.</creator><creator>Palmiere, C.</creator><creator>Lam, L.</creator><creator>Ahn, Y.</creator><creator>Kesha, K.</creator><creator>Stables, S.</creator><creator>Tse, R.</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>0-V</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AM</scope><scope>8AO</scope><scope>8FE</scope><scope>8FG</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ALSLI</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BGRYB</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>HCIFZ</scope><scope>K7.</scope><scope>K9.</scope><scope>L6V</scope><scope>M0O</scope><scope>M0S</scope><scope>M1P</scope><scope>M7S</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>Q9U</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-1388-9537</orcidid></search><sort><creationdate>20190901</creationdate><title>The effects of different sampling techniques on peripheral post mortem tryptase levels: a recommended sampling method</title><author>Garland, J. ; Philcox, W. ; McCarthy, S. ; Hensby-Bennet, S. ; Ondruschka, B. ; Woydt, L. ; Da Broi, U. ; Palmiere, C. ; Lam, L. ; Ahn, Y. ; Kesha, K. ; Stables, S. ; Tse, R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c375t-45206f5592dc00d31f6584fafa3fb6af76ec796219cc3ca08478201984bfce43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Anaphylaxis</topic><topic>Autopsy - methods</topic><topic>Blood</topic><topic>Blood Specimen Collection - methods</topic><topic>Constriction</topic><topic>Female</topic><topic>Femoral Artery</topic><topic>Femoral Vein</topic><topic>Forensic Medicine</topic><topic>Forensic pathology</topic><topic>Forensic Pathology - methods</topic><topic>Humans</topic><topic>Male</topic><topic>Medical Law</topic><topic>Medicine & Public Health</topic><topic>Method Paper</topic><topic>Middle Aged</topic><topic>Outliers (statistics)</topic><topic>Prospective Studies</topic><topic>Sampling methods</topic><topic>Sampling techniques</topic><topic>Statistical methods</topic><topic>Tryptases - blood</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Garland, J.</creatorcontrib><creatorcontrib>Philcox, W.</creatorcontrib><creatorcontrib>McCarthy, S.</creatorcontrib><creatorcontrib>Hensby-Bennet, S.</creatorcontrib><creatorcontrib>Ondruschka, B.</creatorcontrib><creatorcontrib>Woydt, L.</creatorcontrib><creatorcontrib>Da Broi, U.</creatorcontrib><creatorcontrib>Palmiere, C.</creatorcontrib><creatorcontrib>Lam, L.</creatorcontrib><creatorcontrib>Ahn, Y.</creatorcontrib><creatorcontrib>Kesha, K.</creatorcontrib><creatorcontrib>Stables, S.</creatorcontrib><creatorcontrib>Tse, R.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Social Sciences Premium Collection</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Criminal Justice Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Social Science Premium Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Criminology Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Criminal Justice (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Criminal Justice</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Engineering Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><jtitle>International journal of legal medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Garland, J.</au><au>Philcox, W.</au><au>McCarthy, S.</au><au>Hensby-Bennet, S.</au><au>Ondruschka, B.</au><au>Woydt, L.</au><au>Da Broi, U.</au><au>Palmiere, C.</au><au>Lam, L.</au><au>Ahn, Y.</au><au>Kesha, K.</au><au>Stables, S.</au><au>Tse, R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The effects of different sampling techniques on peripheral post mortem tryptase levels: a recommended sampling method</atitle><jtitle>International journal of legal medicine</jtitle><stitle>Int J Legal Med</stitle><addtitle>Int J Legal Med</addtitle><date>2019-09-01</date><risdate>2019</risdate><volume>133</volume><issue>5</issue><spage>1477</spage><epage>1483</epage><pages>1477-1483</pages><issn>0937-9827</issn><eissn>1437-1596</eissn><abstract>Different sampling techniques can impact on post mortem tryptase levels. A previous study demonstrated significantly lower femoral post mortem total tryptase levels in samples collected via transcutaneous aspiration compared with directly sampling during internal examination. However, an outlier with high tryptase level was noted in one transcutaneous aspiration sample. This 6-month prospective study compared total post mortem tryptase levels between 21 paired aspirated venous and arterial femoral blood samples, and 19 paired aspirated and cutdown femoral venous blood samples in non-anaphylactic deaths only. No statistical differences were demonstrated between the different sampling methods. However, four outlier cases with higher tryptase levels in aspirated arterial and femoral cutdown samples compared with aspirated venous femoral samples were noted. The reasons for the outliers may be due to the bloods collected from these two methods being contaminated by central arterial and venous blood with high tryptase levels respectively. None of the aspirated venous femoral post mortem tryptase levels were above recognized post mortem tryptase cutoff to diagnose anaphylaxis. This study recommends aspirating blood samples from a clamped femoral/external iliac vein for post mortem tryptase analysis should be defined as the gold standard. Further study using the recommended sampling method on post mortem tryptase levels in non-anaphylactic and anaphylactic cases is warranted.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>30879133</pmid><doi>10.1007/s00414-019-02038-9</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0002-1388-9537</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0937-9827 |
| ispartof | International journal of legal medicine, 2019-09, Vol.133 (5), p.1477-1483 |
| issn | 0937-9827 1437-1596 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2193605206 |
| source | MEDLINE; HeinOnline Law Journal Library; SpringerLink Journals - AutoHoldings |
| subjects | Adult Aged Aged, 80 and over Anaphylaxis Autopsy - methods Blood Blood Specimen Collection - methods Constriction Female Femoral Artery Femoral Vein Forensic Medicine Forensic pathology Forensic Pathology - methods Humans Male Medical Law Medicine & Public Health Method Paper Middle Aged Outliers (statistics) Prospective Studies Sampling methods Sampling techniques Statistical methods Tryptases - blood Young Adult |
| title | The effects of different sampling techniques on peripheral post mortem tryptase levels: a recommended sampling method |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-08T00%3A34%3A23IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=The%20effects%20of%20different%20sampling%20techniques%20on%20peripheral%20post%20mortem%20tryptase%20levels:%20a%20recommended%20sampling%20method&rft.jtitle=International%20journal%20of%20legal%20medicine&rft.au=Garland,%20J.&rft.date=2019-09-01&rft.volume=133&rft.issue=5&rft.spage=1477&rft.epage=1483&rft.pages=1477-1483&rft.issn=0937-9827&rft.eissn=1437-1596&rft_id=info:doi/10.1007/s00414-019-02038-9&rft_dat=%3Cproquest_cross%3E2191941786%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2191941786&rft_id=info:pmid/30879133&rfr_iscdi=true |