Immunity induced by recombinant attenuated IHNV (infectious hematopoietic necrosis virus)‐GN438A expresses VP2 gene‐encoded IPNV (infectious pancreatic necrosis virus) against both pathogens in rainbow trout
Infectious hematopoietic necrosis virus (IHNV) and infectious pancreatic necrosis virus (IPNV) are important pathogens in rainbow trout farming worldwide. Their co‐infection is also common, which causes great economic loss in juvenile salmon species. Development of a universal virus vaccine providin...
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| Veröffentlicht in: | Journal of fish diseases 2019-05, Vol.42 (5), p.631-642 |
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| creator | Chen, Yaping Guo, Mengting Wang, Yanxue Hua, Xiaojing Gao, Shuai Wang, Yuting Li, Dechuan Shi, Wen Tang, Lijie Li, Yijing Liu, Min |
| description | Infectious hematopoietic necrosis virus (IHNV) and infectious pancreatic necrosis virus (IPNV) are important pathogens in rainbow trout farming worldwide. Their co‐infection is also common, which causes great economic loss in juvenile salmon species. Development of a universal virus vaccine providing broadly cross‐protective immunity will be of great importance. In this study, we generated two recombinant (r) virus (rIHNV‐N438A‐ΔNV‐EGFP and rIHNV‐N438A‐ΔNV‐VP2) replacing the NV gene of the backbone of rIHNV at the single point mutation at residue 438 with an efficient green fluorescent protein (EGFP) reporter gene and antigenic VP2 gene of IPNV. Meanwhile, we tested their efficacy against the wild‐type (wt) IHNV HLJ‐09 virus and IPNV serotype Sp virus challenge. The relative per cent survival rates of two recombinant viruses against (wt) IHNV HLJ‐09 virus challenge were 84.6% and 81.5%, respectively. Simultaneously, the relative per cent survival rate of rIHNV‐N438A‐ΔNV‐VP2 against IPNV serotype Sp virus challenge was 88.9%. It showed the two recombinant viruses had high protection rates and induced a high level of antibodies against IHNV or IPNV. Taken together, these results suggest the VP2 gene of IPNV can act as candidate gene for vaccine and attenuated multivalent live vaccines and molecular marker vaccines have potential application for viral vaccine. |
| doi_str_mv | 10.1111/jfd.12966 |
| format | Article |
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Their co‐infection is also common, which causes great economic loss in juvenile salmon species. Development of a universal virus vaccine providing broadly cross‐protective immunity will be of great importance. In this study, we generated two recombinant (r) virus (rIHNV‐N438A‐ΔNV‐EGFP and rIHNV‐N438A‐ΔNV‐VP2) replacing the NV gene of the backbone of rIHNV at the single point mutation at residue 438 with an efficient green fluorescent protein (EGFP) reporter gene and antigenic VP2 gene of IPNV. Meanwhile, we tested their efficacy against the wild‐type (wt) IHNV HLJ‐09 virus and IPNV serotype Sp virus challenge. The relative per cent survival rates of two recombinant viruses against (wt) IHNV HLJ‐09 virus challenge were 84.6% and 81.5%, respectively. Simultaneously, the relative per cent survival rate of rIHNV‐N438A‐ΔNV‐VP2 against IPNV serotype Sp virus challenge was 88.9%. It showed the two recombinant viruses had high protection rates and induced a high level of antibodies against IHNV or IPNV. Taken together, these results suggest the VP2 gene of IPNV can act as candidate gene for vaccine and attenuated multivalent live vaccines and molecular marker vaccines have potential application for viral vaccine.</description><identifier>ISSN: 0140-7775</identifier><identifier>EISSN: 1365-2761</identifier><identifier>DOI: 10.1111/jfd.12966</identifier><language>eng</language><publisher>Oxford: Blackwell Publishing Ltd</publisher><subject>Antibodies ; Antigens ; Disease control ; Economics ; Fish ; Fluorescence ; Freshwater fishes ; Green fluorescent protein ; Immunity ; immunogenicity ; Infectious diseases ; Infectious hematopoietic necrosis ; Infectious pancreatic necrosis ; infectious pancreatic necrosis virus (IPNV) ; Mutation ; Necrosis ; NV gene ; Oncorhynchus mykiss ; Pancreas ; Pathogens ; Point mutation ; Proteins ; recombinant infectious hematopoietic necrosis virus (rIHNV) ; Recombinants ; Reporter gene ; reverse genetics ; Salmon ; Survival ; Trout ; Vaccines ; Viruses ; VP2 protein</subject><ispartof>Journal of fish diseases, 2019-05, Vol.42 (5), p.631-642</ispartof><rights>2019 John Wiley & Sons Ltd</rights><rights>Copyright © 2019 John Wiley & Sons Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><orcidid>0000-0001-9732-7277</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fjfd.12966$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fjfd.12966$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27903,27904,45553,45554</link.rule.ids></links><search><creatorcontrib>Chen, Yaping</creatorcontrib><creatorcontrib>Guo, Mengting</creatorcontrib><creatorcontrib>Wang, Yanxue</creatorcontrib><creatorcontrib>Hua, Xiaojing</creatorcontrib><creatorcontrib>Gao, Shuai</creatorcontrib><creatorcontrib>Wang, Yuting</creatorcontrib><creatorcontrib>Li, Dechuan</creatorcontrib><creatorcontrib>Shi, Wen</creatorcontrib><creatorcontrib>Tang, Lijie</creatorcontrib><creatorcontrib>Li, Yijing</creatorcontrib><creatorcontrib>Liu, Min</creatorcontrib><title>Immunity induced by recombinant attenuated IHNV (infectious hematopoietic necrosis virus)‐GN438A expresses VP2 gene‐encoded IPNV (infectious pancreatic necrosis virus) against both pathogens in rainbow trout</title><title>Journal of fish diseases</title><description>Infectious hematopoietic necrosis virus (IHNV) and infectious pancreatic necrosis virus (IPNV) are important pathogens in rainbow trout farming worldwide. Their co‐infection is also common, which causes great economic loss in juvenile salmon species. Development of a universal virus vaccine providing broadly cross‐protective immunity will be of great importance. In this study, we generated two recombinant (r) virus (rIHNV‐N438A‐ΔNV‐EGFP and rIHNV‐N438A‐ΔNV‐VP2) replacing the NV gene of the backbone of rIHNV at the single point mutation at residue 438 with an efficient green fluorescent protein (EGFP) reporter gene and antigenic VP2 gene of IPNV. Meanwhile, we tested their efficacy against the wild‐type (wt) IHNV HLJ‐09 virus and IPNV serotype Sp virus challenge. The relative per cent survival rates of two recombinant viruses against (wt) IHNV HLJ‐09 virus challenge were 84.6% and 81.5%, respectively. Simultaneously, the relative per cent survival rate of rIHNV‐N438A‐ΔNV‐VP2 against IPNV serotype Sp virus challenge was 88.9%. It showed the two recombinant viruses had high protection rates and induced a high level of antibodies against IHNV or IPNV. Taken together, these results suggest the VP2 gene of IPNV can act as candidate gene for vaccine and attenuated multivalent live vaccines and molecular marker vaccines have potential application for viral vaccine.</description><subject>Antibodies</subject><subject>Antigens</subject><subject>Disease control</subject><subject>Economics</subject><subject>Fish</subject><subject>Fluorescence</subject><subject>Freshwater fishes</subject><subject>Green fluorescent protein</subject><subject>Immunity</subject><subject>immunogenicity</subject><subject>Infectious diseases</subject><subject>Infectious hematopoietic necrosis</subject><subject>Infectious pancreatic necrosis</subject><subject>infectious pancreatic necrosis virus (IPNV)</subject><subject>Mutation</subject><subject>Necrosis</subject><subject>NV gene</subject><subject>Oncorhynchus mykiss</subject><subject>Pancreas</subject><subject>Pathogens</subject><subject>Point mutation</subject><subject>Proteins</subject><subject>recombinant infectious hematopoietic necrosis virus (rIHNV)</subject><subject>Recombinants</subject><subject>Reporter gene</subject><subject>reverse genetics</subject><subject>Salmon</subject><subject>Survival</subject><subject>Trout</subject><subject>Vaccines</subject><subject>Viruses</subject><subject>VP2 protein</subject><issn>0140-7775</issn><issn>1365-2761</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNptkUFO3DAUhi3USkxpF72BJTawCNhOYjtLBAWmQpQFsLUc55nxaGIH2ymdXY_Qu_UGPUkNdIX6Nn7S_-nTs36EPlNyRMscr-1wRFnH-Q5a0Jq3FROcvkMLQhtSCSHaXfQhpTUhVLSUL9Dv5TjO3uUtdn6YDQy43-IIJoy989pnrHMGP-tckuXl9T0-cN6CyS7MCa9g1DlMwUF2BnswMSSX8HcX53T45-evi-umlicYfkwRUoKE728YfgAPJQNvwvAsvXkjnbQ3EfR_jFg_aOdTxn3Iq8LlVSiyVC7HsQR9eMI5hjl_RO-t3iT49O_dQ3fnX25PL6urbxfL05OraqKE8krIRhLZdr1oSdOQ1g7UtrS2tmegm2YQUvRGMCMJo33Hh06AbaQ2lnLS6UHXe-jg1TvF8DhDymp0ycBmoz2UnyhGu5pyIWVX0P036DrM0ZfrFGNEMMK6lhfq-JV6chvYqim6UcetokQ9d6tKt-qlW_X1_Oxlqf8CZkGepg</recordid><startdate>201905</startdate><enddate>201905</enddate><creator>Chen, Yaping</creator><creator>Guo, Mengting</creator><creator>Wang, Yanxue</creator><creator>Hua, Xiaojing</creator><creator>Gao, Shuai</creator><creator>Wang, Yuting</creator><creator>Li, Dechuan</creator><creator>Shi, Wen</creator><creator>Tang, Lijie</creator><creator>Li, Yijing</creator><creator>Liu, Min</creator><general>Blackwell Publishing Ltd</general><scope>7QL</scope><scope>7TN</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>F1W</scope><scope>FR3</scope><scope>H94</scope><scope>H95</scope><scope>H98</scope><scope>H99</scope><scope>L.F</scope><scope>L.G</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-9732-7277</orcidid></search><sort><creationdate>201905</creationdate><title>Immunity induced by recombinant attenuated IHNV (infectious hematopoietic necrosis virus)‐GN438A expresses VP2 gene‐encoded IPNV (infectious pancreatic necrosis virus) against both pathogens in rainbow trout</title><author>Chen, Yaping ; Guo, Mengting ; Wang, Yanxue ; Hua, Xiaojing ; Gao, Shuai ; Wang, Yuting ; Li, Dechuan ; Shi, Wen ; Tang, Lijie ; Li, Yijing ; Liu, Min</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p1016-78480859b7504405fd1f513ffb2ea44d787bc72c8021b96d97ef48acf1609ada3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Antibodies</topic><topic>Antigens</topic><topic>Disease control</topic><topic>Economics</topic><topic>Fish</topic><topic>Fluorescence</topic><topic>Freshwater fishes</topic><topic>Green fluorescent protein</topic><topic>Immunity</topic><topic>immunogenicity</topic><topic>Infectious diseases</topic><topic>Infectious hematopoietic necrosis</topic><topic>Infectious pancreatic necrosis</topic><topic>infectious pancreatic necrosis virus (IPNV)</topic><topic>Mutation</topic><topic>Necrosis</topic><topic>NV gene</topic><topic>Oncorhynchus mykiss</topic><topic>Pancreas</topic><topic>Pathogens</topic><topic>Point mutation</topic><topic>Proteins</topic><topic>recombinant infectious hematopoietic necrosis virus (rIHNV)</topic><topic>Recombinants</topic><topic>Reporter gene</topic><topic>reverse genetics</topic><topic>Salmon</topic><topic>Survival</topic><topic>Trout</topic><topic>Vaccines</topic><topic>Viruses</topic><topic>VP2 protein</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chen, Yaping</creatorcontrib><creatorcontrib>Guo, Mengting</creatorcontrib><creatorcontrib>Wang, Yanxue</creatorcontrib><creatorcontrib>Hua, Xiaojing</creatorcontrib><creatorcontrib>Gao, Shuai</creatorcontrib><creatorcontrib>Wang, Yuting</creatorcontrib><creatorcontrib>Li, Dechuan</creatorcontrib><creatorcontrib>Shi, Wen</creatorcontrib><creatorcontrib>Tang, Lijie</creatorcontrib><creatorcontrib>Li, Yijing</creatorcontrib><creatorcontrib>Liu, Min</creatorcontrib><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Oceanic Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Aquaculture Abstracts</collection><collection>ASFA: Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of fish diseases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chen, Yaping</au><au>Guo, Mengting</au><au>Wang, Yanxue</au><au>Hua, Xiaojing</au><au>Gao, Shuai</au><au>Wang, Yuting</au><au>Li, Dechuan</au><au>Shi, Wen</au><au>Tang, Lijie</au><au>Li, Yijing</au><au>Liu, Min</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Immunity induced by recombinant attenuated IHNV (infectious hematopoietic necrosis virus)‐GN438A expresses VP2 gene‐encoded IPNV (infectious pancreatic necrosis virus) against both pathogens in rainbow trout</atitle><jtitle>Journal of fish diseases</jtitle><date>2019-05</date><risdate>2019</risdate><volume>42</volume><issue>5</issue><spage>631</spage><epage>642</epage><pages>631-642</pages><issn>0140-7775</issn><eissn>1365-2761</eissn><abstract>Infectious hematopoietic necrosis virus (IHNV) and infectious pancreatic necrosis virus (IPNV) are important pathogens in rainbow trout farming worldwide. Their co‐infection is also common, which causes great economic loss in juvenile salmon species. Development of a universal virus vaccine providing broadly cross‐protective immunity will be of great importance. In this study, we generated two recombinant (r) virus (rIHNV‐N438A‐ΔNV‐EGFP and rIHNV‐N438A‐ΔNV‐VP2) replacing the NV gene of the backbone of rIHNV at the single point mutation at residue 438 with an efficient green fluorescent protein (EGFP) reporter gene and antigenic VP2 gene of IPNV. Meanwhile, we tested their efficacy against the wild‐type (wt) IHNV HLJ‐09 virus and IPNV serotype Sp virus challenge. The relative per cent survival rates of two recombinant viruses against (wt) IHNV HLJ‐09 virus challenge were 84.6% and 81.5%, respectively. Simultaneously, the relative per cent survival rate of rIHNV‐N438A‐ΔNV‐VP2 against IPNV serotype Sp virus challenge was 88.9%. It showed the two recombinant viruses had high protection rates and induced a high level of antibodies against IHNV or IPNV. Taken together, these results suggest the VP2 gene of IPNV can act as candidate gene for vaccine and attenuated multivalent live vaccines and molecular marker vaccines have potential application for viral vaccine.</abstract><cop>Oxford</cop><pub>Blackwell Publishing Ltd</pub><doi>10.1111/jfd.12966</doi><tpages>12</tpages><orcidid>https://orcid.org/0000-0001-9732-7277</orcidid></addata></record> |
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| subjects | Antibodies Antigens Disease control Economics Fish Fluorescence Freshwater fishes Green fluorescent protein Immunity immunogenicity Infectious diseases Infectious hematopoietic necrosis Infectious pancreatic necrosis infectious pancreatic necrosis virus (IPNV) Mutation Necrosis NV gene Oncorhynchus mykiss Pancreas Pathogens Point mutation Proteins recombinant infectious hematopoietic necrosis virus (rIHNV) Recombinants Reporter gene reverse genetics Salmon Survival Trout Vaccines Viruses VP2 protein |
| title | Immunity induced by recombinant attenuated IHNV (infectious hematopoietic necrosis virus)‐GN438A expresses VP2 gene‐encoded IPNV (infectious pancreatic necrosis virus) against both pathogens in rainbow trout |
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