Colorimetric determination of total protein content in serum based on the polydopamine/protein adsorption competition on microplates

We report a facile, low-cost and safe analytical method for estimation of total protein content, even in complex matrix like human serum, by exploiting the competition between proteins and polydopamine (PDA) for surface binding. The surface coating has been examined by using a microplate reader taki...

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Veröffentlicht in:	Talanta (Oxford) 2019-06, Vol.198, p.15-22
Hauptverfasser:	Palladino, Pasquale, Brittoli, Alvaro, Pascale, Emanuela, Minunni, Maria, Scarano, Simona
Format:	Artikel
Sprache:	eng
Schlagworte:	Adsorption competition Adsorption isotherms Colorimetric assay Polydopamine coating Total protein content
Online-Zugang:	Volltext
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