Colorimetric determination of total protein content in serum based on the polydopamine/protein adsorption competition on microplates
We report a facile, low-cost and safe analytical method for estimation of total protein content, even in complex matrix like human serum, by exploiting the competition between proteins and polydopamine (PDA) for surface binding. The surface coating has been examined by using a microplate reader taki...
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| Veröffentlicht in: | Talanta (Oxford) 2019-06, Vol.198, p.15-22 |
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| creator | Palladino, Pasquale Brittoli, Alvaro Pascale, Emanuela Minunni, Maria Scarano, Simona |
| description | We report a facile, low-cost and safe analytical method for estimation of total protein content, even in complex matrix like human serum, by exploiting the competition between proteins and polydopamine (PDA) for surface binding. The surface coating has been examined by using a microplate reader taking the advantage of the PDA absorbance in the visible region, obtaining new insights into the modelling of polydopamine deposition and polymer/protein adsorption competition. This is helpful for rational development of imprinted biosensors, and potentially offering a with broad applications ranging from diagnostic tools in medicine to food analysis. The isothermal adsorption of polydopamine on polystyrene surface of multi-well plate displays a Langmuir-shaped curve. It allows the determination of the parameters of polymer film formation useful for any analytical assay depending on the surface coating. Among these, the molecular imprinting and the optical and acoustic evanescent sensing.
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•Colorimetric determination of total protein content in serum.•Langmuir model for isothermal biopolymer film formation.•Polydopamine/protein adsorption competition on microplates. |
| doi_str_mv | 10.1016/j.talanta.2019.01.095 |
| format | Article |
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[Display omitted]
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[Display omitted]
•Colorimetric determination of total protein content in serum.•Langmuir model for isothermal biopolymer film formation.•Polydopamine/protein adsorption competition on microplates.</description><subject>Adsorption competition</subject><subject>Adsorption isotherms</subject><subject>Colorimetric assay</subject><subject>Polydopamine coating</subject><subject>Total protein content</subject><issn>0039-9140</issn><issn>1873-3573</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNqFUblu3DAQJYIY8fr4hAQs00geiqIkVkGwiA_AgBu7JihyhHAhiQpJBdg-H246u-vW1Uzxjpn3CPnKoGTAmptdmfSo56TLCpgsgZUgxSeyYV3LCy5a_plsALgsJKvhnFzEuAOAigP_Qs45dG0jar4h_7Z-9MFNmIIz1GLCMLlZJ-dn6geafHahS_AJ3UyNnxPOieY1Ylgn2uuIlmZo-o108ePe-kVnPt6cKNpGH5b_csZPCyZ3kJ7p5Ezwy6gTxityNugx4vVxXpKX21_P2_vi8enuYfvzsTA1q1JhTK_rSkvEwVYgOt4hirqxXddJXTPJRN9L08NghAHZc8nBohVVO4jBNMLwS_L9oJuv-7NiTGpy0eCYc0S_RlUxyVlTi1ZmqDhA85ExBhzUklPSYa8YqLcC1E4dC1BvBShgKheQed-OFms_oX1nnRLPgB8HAOZH_zoMKhqHs0HrApqkrHcfWLwCqteegw</recordid><startdate>20190601</startdate><enddate>20190601</enddate><creator>Palladino, Pasquale</creator><creator>Brittoli, Alvaro</creator><creator>Pascale, Emanuela</creator><creator>Minunni, Maria</creator><creator>Scarano, Simona</creator><general>Elsevier B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-0906-0862</orcidid><orcidid>https://orcid.org/0000-0002-8050-1715</orcidid></search><sort><creationdate>20190601</creationdate><title>Colorimetric determination of total protein content in serum based on the polydopamine/protein adsorption competition on microplates</title><author>Palladino, Pasquale ; Brittoli, Alvaro ; Pascale, Emanuela ; Minunni, Maria ; Scarano, Simona</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c412t-ccba42a9eefd205838ee546d8889a41915bb9cb0fc5c09b3930ded527f5fc65c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Adsorption competition</topic><topic>Adsorption isotherms</topic><topic>Colorimetric assay</topic><topic>Polydopamine coating</topic><topic>Total protein content</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Palladino, Pasquale</creatorcontrib><creatorcontrib>Brittoli, Alvaro</creatorcontrib><creatorcontrib>Pascale, Emanuela</creatorcontrib><creatorcontrib>Minunni, Maria</creatorcontrib><creatorcontrib>Scarano, Simona</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Talanta (Oxford)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Palladino, Pasquale</au><au>Brittoli, Alvaro</au><au>Pascale, Emanuela</au><au>Minunni, Maria</au><au>Scarano, Simona</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Colorimetric determination of total protein content in serum based on the polydopamine/protein adsorption competition on microplates</atitle><jtitle>Talanta (Oxford)</jtitle><addtitle>Talanta</addtitle><date>2019-06-01</date><risdate>2019</risdate><volume>198</volume><spage>15</spage><epage>22</epage><pages>15-22</pages><issn>0039-9140</issn><eissn>1873-3573</eissn><abstract>We report a facile, low-cost and safe analytical method for estimation of total protein content, even in complex matrix like human serum, by exploiting the competition between proteins and polydopamine (PDA) for surface binding. The surface coating has been examined by using a microplate reader taking the advantage of the PDA absorbance in the visible region, obtaining new insights into the modelling of polydopamine deposition and polymer/protein adsorption competition. This is helpful for rational development of imprinted biosensors, and potentially offering a with broad applications ranging from diagnostic tools in medicine to food analysis. The isothermal adsorption of polydopamine on polystyrene surface of multi-well plate displays a Langmuir-shaped curve. It allows the determination of the parameters of polymer film formation useful for any analytical assay depending on the surface coating. Among these, the molecular imprinting and the optical and acoustic evanescent sensing.
[Display omitted]
•Colorimetric determination of total protein content in serum.•Langmuir model for isothermal biopolymer film formation.•Polydopamine/protein adsorption competition on microplates.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>30876543</pmid><doi>10.1016/j.talanta.2019.01.095</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0003-0906-0862</orcidid><orcidid>https://orcid.org/0000-0002-8050-1715</orcidid><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0039-9140 |
| ispartof | Talanta (Oxford), 2019-06, Vol.198, p.15-22 |
| issn | 0039-9140 1873-3573 |
| language | eng |
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| source | Elsevier ScienceDirect Journals Complete |
| subjects | Adsorption competition Adsorption isotherms Colorimetric assay Polydopamine coating Total protein content |
| title | Colorimetric determination of total protein content in serum based on the polydopamine/protein adsorption competition on microplates |
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