Identification of upstream culture conditions and harvest time parameters that affect host cell protein clearance

During early stage bioprocess development, characterizing interactions between unit operations is a key challenge. Such interactions include the release of host cell enzymes early in the process causing losses in product quality downstream. Using a CHO‐expressed IgG1 system, the impact of cell cultu...

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Veröffentlicht in:	Biotechnology progress 2019-05, Vol.35 (3), p.e2805-n/a
Hauptverfasser:	Wilson, Louisa J., Lewis, Will, Kucia‐Tran, Richard, Bracewell, Daniel G.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antibodies Bioreactors Cell culture Cell Culture Techniques - methods Centrifugation CHO Cells Chromatography Chromatography, Affinity - methods Cricetinae Cricetulus Cultivation Developmental stages Downstream downstream processing host cell proteins Immunoglobulin G Immunoglobulin G - isolation & purification Immunoglobulin G - metabolism Monoclonal antibodies Optimization Parameter identification Polishing Product quality Protein A Protein purification Proteins Purification Shear strength Staphylococcal Protein A - chemistry Time Factors Upstream upstream processing
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