On the occurrence and molecular identification of Contracaecum larvae (Nematoda: Anisakidae) in Mugil cephalus from Turkish waters
Anisakis and Contracaecum species are fish borne zoonotic nematodes. In our previous studies, other larval anisakid and raphidascarid nematodes, Anisakis and Hysterothylacium species, were genetically identified in marine fish from Turkish waters. However, there is no information on molecular identi...
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| Veröffentlicht in: | Parasitology research (1987) 2019-05, Vol.118 (5), p.1393-1402 |
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| creator | Pekmezci, Gokmen Zafer Yardimci, Banu |
| description | Anisakis
and
Contracaecum
species are fish borne zoonotic nematodes. In our previous studies, other larval anisakid and raphidascarid nematodes,
Anisakis
and
Hysterothylacium
species, were genetically identified in marine fish from Turkish waters. However, there is no information on molecular identification of larval
Contracaecum
species in marine fish from Turkey. Therefore, the aim of this study was only to investigate the presence and molecular identification of
Contracaecum
species in commonly commercialized marine fish from Turkish waters. A total of 475 marine fish, which belong to 21 different species, were sampled from the Aegean (FAO 37.3.1), Mediterranean (FAO 37.3.2), and Black Sea (FAO 37.4.2). The prevalence of
Contracaecum
L3 larvae in the Aegean Sea was identified as 10% in
Mugil cephalus
. All
Contracaecum
L3 larvae were molecularly characterized with RFLP targeting the ITS region and
rrnS
gene. Moreover, all larvae were analyzed by sequencing of ITS region,
rrnS
and
cox2
gene. All
Contracaecum
larvae were identified as
C. overstreeti
based on the
cox2
sequence analysis. This is the first report of
C. overstreeti
larvae in
M. cephalus
as paratenic and intermediate hosts. Furthermore, the analysis reveals novel information on ITS region. Additionally, the
rrnS
gene of
C. overstreeti
was also achieved and deposited in Genbank for the first time. The PCR-RFLP patterns of the ITS region and
rrnS
gene from
C. overstreeti
were presented in the present study. Consequently, the presence of
C. overstreeti
larvae in
M. cephalus
from the Aegean Sea may also potentially capable of inducing allergic sensitization in humans. |
| doi_str_mv | 10.1007/s00436-019-06278-x |
| format | Article |
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and
Contracaecum
species are fish borne zoonotic nematodes. In our previous studies, other larval anisakid and raphidascarid nematodes,
Anisakis
and
Hysterothylacium
species, were genetically identified in marine fish from Turkish waters. However, there is no information on molecular identification of larval
Contracaecum
species in marine fish from Turkey. Therefore, the aim of this study was only to investigate the presence and molecular identification of
Contracaecum
species in commonly commercialized marine fish from Turkish waters. A total of 475 marine fish, which belong to 21 different species, were sampled from the Aegean (FAO 37.3.1), Mediterranean (FAO 37.3.2), and Black Sea (FAO 37.4.2). The prevalence of
Contracaecum
L3 larvae in the Aegean Sea was identified as 10% in
Mugil cephalus
. All
Contracaecum
L3 larvae were molecularly characterized with RFLP targeting the ITS region and
rrnS
gene. Moreover, all larvae were analyzed by sequencing of ITS region,
rrnS
and
cox2
gene. All
Contracaecum
larvae were identified as
C. overstreeti
based on the
cox2
sequence analysis. This is the first report of
C. overstreeti
larvae in
M. cephalus
as paratenic and intermediate hosts. Furthermore, the analysis reveals novel information on ITS region. Additionally, the
rrnS
gene of
C. overstreeti
was also achieved and deposited in Genbank for the first time. The PCR-RFLP patterns of the ITS region and
rrnS
gene from
C. overstreeti
were presented in the present study. Consequently, the presence of
C. overstreeti
larvae in
M. cephalus
from the Aegean Sea may also potentially capable of inducing allergic sensitization in humans. </description><identifier>ISSN: 0932-0113</identifier><identifier>EISSN: 1432-1955</identifier><identifier>DOI: 10.1007/s00436-019-06278-x</identifier><identifier>PMID: 30863896</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Anisakidae ; Anisakis ; Biomedical and Life Sciences ; Biomedicine ; Contamination ; Contracaecum ; Cyclooxygenase-2 ; Fish ; Fish Parasitology - Original Paper ; Flathead mullet ; Identification and classification ; Immunology ; Medical Microbiology ; Microbiology ; Molecular genetics ; Mugil cephalus ; Nematodes ; Parasitological research ; Polymerase chain reaction ; Restriction fragment length polymorphism ; RrnS gene ; Species</subject><ispartof>Parasitology research (1987), 2019-05, Vol.118 (5), p.1393-1402</ispartof><rights>Springer-Verlag GmbH Germany, part of Springer Nature 2019</rights><rights>COPYRIGHT 2019 Springer</rights><rights>Copyright Springer Nature B.V. 2019</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c414t-70609e9d879434178d3bd3cb4673b285d2aaf409e79bed62bff1e4ce8424d583</citedby><cites>FETCH-LOGICAL-c414t-70609e9d879434178d3bd3cb4673b285d2aaf409e79bed62bff1e4ce8424d583</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00436-019-06278-x$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00436-019-06278-x$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27923,27924,41487,42556,51318</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30863896$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pekmezci, Gokmen Zafer</creatorcontrib><creatorcontrib>Yardimci, Banu</creatorcontrib><title>On the occurrence and molecular identification of Contracaecum larvae (Nematoda: Anisakidae) in Mugil cephalus from Turkish waters</title><title>Parasitology research (1987)</title><addtitle>Parasitol Res</addtitle><addtitle>Parasitol Res</addtitle><description>Anisakis
and
Contracaecum
species are fish borne zoonotic nematodes. In our previous studies, other larval anisakid and raphidascarid nematodes,
Anisakis
and
Hysterothylacium
species, were genetically identified in marine fish from Turkish waters. However, there is no information on molecular identification of larval
Contracaecum
species in marine fish from Turkey. Therefore, the aim of this study was only to investigate the presence and molecular identification of
Contracaecum
species in commonly commercialized marine fish from Turkish waters. A total of 475 marine fish, which belong to 21 different species, were sampled from the Aegean (FAO 37.3.1), Mediterranean (FAO 37.3.2), and Black Sea (FAO 37.4.2). The prevalence of
Contracaecum
L3 larvae in the Aegean Sea was identified as 10% in
Mugil cephalus
. All
Contracaecum
L3 larvae were molecularly characterized with RFLP targeting the ITS region and
rrnS
gene. Moreover, all larvae were analyzed by sequencing of ITS region,
rrnS
and
cox2
gene. All
Contracaecum
larvae were identified as
C. overstreeti
based on the
cox2
sequence analysis. This is the first report of
C. overstreeti
larvae in
M. cephalus
as paratenic and intermediate hosts. Furthermore, the analysis reveals novel information on ITS region. Additionally, the
rrnS
gene of
C. overstreeti
was also achieved and deposited in Genbank for the first time. The PCR-RFLP patterns of the ITS region and
rrnS
gene from
C. overstreeti
were presented in the present study. Consequently, the presence of
C. overstreeti
larvae in
M. cephalus
from the Aegean Sea may also potentially capable of inducing allergic sensitization in humans. </description><subject>Anisakidae</subject><subject>Anisakis</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Contamination</subject><subject>Contracaecum</subject><subject>Cyclooxygenase-2</subject><subject>Fish</subject><subject>Fish Parasitology - Original Paper</subject><subject>Flathead mullet</subject><subject>Identification and classification</subject><subject>Immunology</subject><subject>Medical Microbiology</subject><subject>Microbiology</subject><subject>Molecular genetics</subject><subject>Mugil cephalus</subject><subject>Nematodes</subject><subject>Parasitological research</subject><subject>Polymerase chain reaction</subject><subject>Restriction fragment length polymorphism</subject><subject>RrnS gene</subject><subject>Species</subject><issn>0932-0113</issn><issn>1432-1955</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNp9kT1vFDEQhi0EIkfgD1AgSzSh2OCv213TnU58SYE011tee3znZNc-7HUILb8cJxtAoqCyZT_vzGgehF5Sck4J6d5mQgRvG0JlQ1rW9c3tI7SigrOGyvX6MVoRWe-EUn6CnuV8RQjtWiGeohNO-pb3sl2hn5cBzwfA0ZiSEgQDWAeLpziCKaNO2FsIs3fe6NnHgKPD2xjmpI2uwIQrcqMBn32FSc_R6nd4E3zW195qeIN9wF_K3o_YwPGgx5KxS3HCu5KufT7g73qGlJ-jJ06PGV48nKdo9-H9bvupubj8-Hm7uWiMoGJuOtISCdL2nRRc0K63fLDcDKLt-MD6tWVaO1GRTg5gWzY4R0EY6AUTdt3zU3S2lD2m-K1AntXks4Fx1AFiyYpRSeuuBL1DX_-DXsWSQh1OMUY5laxnpFLnC7XXIygfXLxfi9EWJm9iAOfr-6a2FoxXUzXAloBJMecETh2Tn3T6oShRd0bVYlRVo-reqLqtoVcPs5RhAvsn8lthBfgC5PoV9pD-Dvufsr8Aqk-srg</recordid><startdate>20190501</startdate><enddate>20190501</enddate><creator>Pekmezci, Gokmen Zafer</creator><creator>Yardimci, Banu</creator><general>Springer Berlin Heidelberg</general><general>Springer</general><general>Springer Nature B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20190501</creationdate><title>On the occurrence and molecular identification of Contracaecum larvae (Nematoda: Anisakidae) in Mugil cephalus from Turkish waters</title><author>Pekmezci, Gokmen Zafer ; Yardimci, Banu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c414t-70609e9d879434178d3bd3cb4673b285d2aaf409e79bed62bff1e4ce8424d583</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Anisakidae</topic><topic>Anisakis</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Contamination</topic><topic>Contracaecum</topic><topic>Cyclooxygenase-2</topic><topic>Fish</topic><topic>Fish Parasitology - Original Paper</topic><topic>Flathead mullet</topic><topic>Identification and classification</topic><topic>Immunology</topic><topic>Medical Microbiology</topic><topic>Microbiology</topic><topic>Molecular genetics</topic><topic>Mugil cephalus</topic><topic>Nematodes</topic><topic>Parasitological research</topic><topic>Polymerase chain reaction</topic><topic>Restriction fragment length polymorphism</topic><topic>RrnS gene</topic><topic>Species</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pekmezci, Gokmen Zafer</creatorcontrib><creatorcontrib>Yardimci, Banu</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Parasitology research (1987)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pekmezci, Gokmen Zafer</au><au>Yardimci, Banu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>On the occurrence and molecular identification of Contracaecum larvae (Nematoda: Anisakidae) in Mugil cephalus from Turkish waters</atitle><jtitle>Parasitology research (1987)</jtitle><stitle>Parasitol Res</stitle><addtitle>Parasitol Res</addtitle><date>2019-05-01</date><risdate>2019</risdate><volume>118</volume><issue>5</issue><spage>1393</spage><epage>1402</epage><pages>1393-1402</pages><issn>0932-0113</issn><eissn>1432-1955</eissn><abstract>Anisakis
and
Contracaecum
species are fish borne zoonotic nematodes. In our previous studies, other larval anisakid and raphidascarid nematodes,
Anisakis
and
Hysterothylacium
species, were genetically identified in marine fish from Turkish waters. However, there is no information on molecular identification of larval
Contracaecum
species in marine fish from Turkey. Therefore, the aim of this study was only to investigate the presence and molecular identification of
Contracaecum
species in commonly commercialized marine fish from Turkish waters. A total of 475 marine fish, which belong to 21 different species, were sampled from the Aegean (FAO 37.3.1), Mediterranean (FAO 37.3.2), and Black Sea (FAO 37.4.2). The prevalence of
Contracaecum
L3 larvae in the Aegean Sea was identified as 10% in
Mugil cephalus
. All
Contracaecum
L3 larvae were molecularly characterized with RFLP targeting the ITS region and
rrnS
gene. Moreover, all larvae were analyzed by sequencing of ITS region,
rrnS
and
cox2
gene. All
Contracaecum
larvae were identified as
C. overstreeti
based on the
cox2
sequence analysis. This is the first report of
C. overstreeti
larvae in
M. cephalus
as paratenic and intermediate hosts. Furthermore, the analysis reveals novel information on ITS region. Additionally, the
rrnS
gene of
C. overstreeti
was also achieved and deposited in Genbank for the first time. The PCR-RFLP patterns of the ITS region and
rrnS
gene from
C. overstreeti
were presented in the present study. Consequently, the presence of
C. overstreeti
larvae in
M. cephalus
from the Aegean Sea may also potentially capable of inducing allergic sensitization in humans. </abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>30863896</pmid><doi>10.1007/s00436-019-06278-x</doi><tpages>10</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0932-0113 |
| ispartof | Parasitology research (1987), 2019-05, Vol.118 (5), p.1393-1402 |
| issn | 0932-0113 1432-1955 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2191011418 |
| source | SpringerLink Journals - AutoHoldings |
| subjects | Anisakidae Anisakis Biomedical and Life Sciences Biomedicine Contamination Contracaecum Cyclooxygenase-2 Fish Fish Parasitology - Original Paper Flathead mullet Identification and classification Immunology Medical Microbiology Microbiology Molecular genetics Mugil cephalus Nematodes Parasitological research Polymerase chain reaction Restriction fragment length polymorphism RrnS gene Species |
| title | On the occurrence and molecular identification of Contracaecum larvae (Nematoda: Anisakidae) in Mugil cephalus from Turkish waters |
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