Reverse-transcription polymerase chain reaction detection of citrus tristeza virus in aphids
A rapid and simple reverse-transcription polymerase chain reaction (RT-PCR) method was developed for the detection of citrus tristeza virus (CTV) in three aphid species. Seven CTV isolates from a worldwide isolate collection were used for aphid acquisition feeding by three aphid species. These inclu...
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| Veröffentlicht in: | Plant disease 1997-09, Vol.81 (9), p.1066-1069 |
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| creator | Mehta, P Brlansky, R.H Gowda, S Yokomi, R.K |
| description | A rapid and simple reverse-transcription polymerase chain reaction (RT-PCR) method was developed for the detection of citrus tristeza virus (CTV) in three aphid species. Seven CTV isolates from a worldwide isolate collection were used for aphid acquisition feeding by three aphid species. These included the most efficient CTV vector, the brown citrus aphid, Toxoptera citricida; the melon aphid, Aphis gossypii; and the green peach aphid, Myzus persicae, a non-vector for CTV. A short procedure for nucleic acid extraction from single or groups of aphids was developed. Nucleic acid extracts from 1, 3, 5, and 10 aphids with acquisition-access periods of 24 and 48 h were reverse transcribed and amplified using primers for the coat protein gene of the Florida B3 (T-36) isolate of CTV. PCR-amplified fragments of approximately 670 bp were obtained from all the isolates tested and the amplified product from the aphids fed on citrus infected with isolate B3 was confirmed as the CTV coat protein gene by digesting with various restriction enzymes. This technique will be useful in investigations of CTV-vector-plant interactions and CTV epidemiology |
| doi_str_mv | 10.1094/PDIS.1997.81.9.1066 |
| format | Article |
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Seven CTV isolates from a worldwide isolate collection were used for aphid acquisition feeding by three aphid species. These included the most efficient CTV vector, the brown citrus aphid, Toxoptera citricida; the melon aphid, Aphis gossypii; and the green peach aphid, Myzus persicae, a non-vector for CTV. A short procedure for nucleic acid extraction from single or groups of aphids was developed. Nucleic acid extracts from 1, 3, 5, and 10 aphids with acquisition-access periods of 24 and 48 h were reverse transcribed and amplified using primers for the coat protein gene of the Florida B3 (T-36) isolate of CTV. PCR-amplified fragments of approximately 670 bp were obtained from all the isolates tested and the amplified product from the aphids fed on citrus infected with isolate B3 was confirmed as the CTV coat protein gene by digesting with various restriction enzymes. This technique will be useful in investigations of CTV-vector-plant interactions and CTV epidemiology</description><identifier>ISSN: 0191-2917</identifier><identifier>EISSN: 1943-7692</identifier><identifier>DOI: 10.1094/PDIS.1997.81.9.1066</identifier><identifier>PMID: 30861961</identifier><identifier>CODEN: PLDIDE</identifier><language>eng</language><publisher>St. Paul, MN: American Phytopathological Society</publisher><subject>APHIS GOSSYPII ; Biological and medical sciences ; CITRUS ; CITRUS TRISTEZA CLOSTEROVIRUS ; CLOSTEROVIRUS TRISTEZA DEL CITRUS ; CLOSTEROVIRUS TRISTEZA DU CITRUS ; COAT PROTEINS ; DISEASE VECTORS ; Fundamental and applied biological sciences. Psychology ; GENE ; Generalities. Techniques. Transmission, epidemiology, ecology. Antiviral substances, control ; GENES ; MICROBIAL PROTEINS ; MYZUS PERSICAE ; PCR ; Phytopathology. Animal pests. Plant and forest protection ; Plant viruses and viroids ; PROTEINAS MICROBIANAS ; PROTEINE MICROBIENNE ; REVERSE TRANSCRIPTION ; STRUCTURAL GENES ; TOXOPTERA CITRICIDUS ; TRANSCRIPCION INVERSA ; TRANSCRIPTION INVERSE ; VECTEUR DE MALADIE ; VECTORES ; VECTORS</subject><ispartof>Plant disease, 1997-09, Vol.81 (9), p.1066-1069</ispartof><rights>1997 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c430t-ccb04d369ec78cf2ed57959852a39d3ba78029e6e09269f8217b5b860ad179c53</citedby><cites>FETCH-LOGICAL-c430t-ccb04d369ec78cf2ed57959852a39d3ba78029e6e09269f8217b5b860ad179c53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,3724,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2830245$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30861961$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mehta, P</creatorcontrib><creatorcontrib>Brlansky, R.H</creatorcontrib><creatorcontrib>Gowda, S</creatorcontrib><creatorcontrib>Yokomi, R.K</creatorcontrib><title>Reverse-transcription polymerase chain reaction detection of citrus tristeza virus in aphids</title><title>Plant disease</title><addtitle>Plant Dis</addtitle><description>A rapid and simple reverse-transcription polymerase chain reaction (RT-PCR) method was developed for the detection of citrus tristeza virus (CTV) in three aphid species. Seven CTV isolates from a worldwide isolate collection were used for aphid acquisition feeding by three aphid species. These included the most efficient CTV vector, the brown citrus aphid, Toxoptera citricida; the melon aphid, Aphis gossypii; and the green peach aphid, Myzus persicae, a non-vector for CTV. A short procedure for nucleic acid extraction from single or groups of aphids was developed. Nucleic acid extracts from 1, 3, 5, and 10 aphids with acquisition-access periods of 24 and 48 h were reverse transcribed and amplified using primers for the coat protein gene of the Florida B3 (T-36) isolate of CTV. PCR-amplified fragments of approximately 670 bp were obtained from all the isolates tested and the amplified product from the aphids fed on citrus infected with isolate B3 was confirmed as the CTV coat protein gene by digesting with various restriction enzymes. This technique will be useful in investigations of CTV-vector-plant interactions and CTV epidemiology</description><subject>APHIS GOSSYPII</subject><subject>Biological and medical sciences</subject><subject>CITRUS</subject><subject>CITRUS TRISTEZA CLOSTEROVIRUS</subject><subject>CLOSTEROVIRUS TRISTEZA DEL CITRUS</subject><subject>CLOSTEROVIRUS TRISTEZA DU CITRUS</subject><subject>COAT PROTEINS</subject><subject>DISEASE VECTORS</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>GENE</subject><subject>Generalities. Techniques. Transmission, epidemiology, ecology. Antiviral substances, control</subject><subject>GENES</subject><subject>MICROBIAL PROTEINS</subject><subject>MYZUS PERSICAE</subject><subject>PCR</subject><subject>Phytopathology. Animal pests. Plant and forest protection</subject><subject>Plant viruses and viroids</subject><subject>PROTEINAS MICROBIANAS</subject><subject>PROTEINE MICROBIENNE</subject><subject>REVERSE TRANSCRIPTION</subject><subject>STRUCTURAL GENES</subject><subject>TOXOPTERA CITRICIDUS</subject><subject>TRANSCRIPCION INVERSA</subject><subject>TRANSCRIPTION INVERSE</subject><subject>VECTEUR DE MALADIE</subject><subject>VECTORES</subject><subject>VECTORS</subject><issn>0191-2917</issn><issn>1943-7692</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><recordid>eNp9kE1P3DAQhi1UBFvKL6ha5YCqXrId24ntOSL6hYTUqsANyXKcSXGV3QQ7iwS_vg67cOTkseeZd-SHsfcclhyw-vL76_nlkiPqpeFLzG9K7bEFx0qWWqF4wxbAkZcCuT5kb1P6BwBVpcwBO5RgFEfFF-zmD91TTFRO0a2Tj2GcwrAuxqF_WFF0iQp_68K6iOT8U6elibbV0BU-THGTiimGNNGjK-7DfM24G29Dm96x_c71iY535xG7_v7t6uxnefHrx_nZ6UXpKwlT6X0DVSsVktfGd4LaWmONphZOYisbpw0IJEWAQmFnBNdN3RgFruUafS2P2Odt7hiHuw2lya5C8tT3bk3DJlmRPQAggM7op1dRruosR_IMyi3o45BSpM6OMaxcfLAc7Ozfzv7t7N8abtHO_vPUx138pllR-zLzLDwDJzvAJe_6Llv3Ib1wwkgQ1fyjD1usc4N1f7Nfe30574LsBaT8DyeXl5E</recordid><startdate>19970901</startdate><enddate>19970901</enddate><creator>Mehta, P</creator><creator>Brlansky, R.H</creator><creator>Gowda, S</creator><creator>Yokomi, R.K</creator><general>American Phytopathological Society</general><scope>FBQ</scope><scope>IQODW</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SS</scope><scope>7T7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19970901</creationdate><title>Reverse-transcription polymerase chain reaction detection of citrus tristeza virus in aphids</title><author>Mehta, P ; Brlansky, R.H ; Gowda, S ; Yokomi, R.K</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c430t-ccb04d369ec78cf2ed57959852a39d3ba78029e6e09269f8217b5b860ad179c53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>APHIS GOSSYPII</topic><topic>Biological and medical sciences</topic><topic>CITRUS</topic><topic>CITRUS TRISTEZA CLOSTEROVIRUS</topic><topic>CLOSTEROVIRUS TRISTEZA DEL CITRUS</topic><topic>CLOSTEROVIRUS TRISTEZA DU CITRUS</topic><topic>COAT PROTEINS</topic><topic>DISEASE VECTORS</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>GENE</topic><topic>Generalities. Techniques. Transmission, epidemiology, ecology. Antiviral substances, control</topic><topic>GENES</topic><topic>MICROBIAL PROTEINS</topic><topic>MYZUS PERSICAE</topic><topic>PCR</topic><topic>Phytopathology. Animal pests. Plant and forest protection</topic><topic>Plant viruses and viroids</topic><topic>PROTEINAS MICROBIANAS</topic><topic>PROTEINE MICROBIENNE</topic><topic>REVERSE TRANSCRIPTION</topic><topic>STRUCTURAL GENES</topic><topic>TOXOPTERA CITRICIDUS</topic><topic>TRANSCRIPCION INVERSA</topic><topic>TRANSCRIPTION INVERSE</topic><topic>VECTEUR DE MALADIE</topic><topic>VECTORES</topic><topic>VECTORS</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mehta, P</creatorcontrib><creatorcontrib>Brlansky, R.H</creatorcontrib><creatorcontrib>Gowda, S</creatorcontrib><creatorcontrib>Yokomi, R.K</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Plant disease</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mehta, P</au><au>Brlansky, R.H</au><au>Gowda, S</au><au>Yokomi, R.K</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Reverse-transcription polymerase chain reaction detection of citrus tristeza virus in aphids</atitle><jtitle>Plant disease</jtitle><addtitle>Plant Dis</addtitle><date>1997-09-01</date><risdate>1997</risdate><volume>81</volume><issue>9</issue><spage>1066</spage><epage>1069</epage><pages>1066-1069</pages><issn>0191-2917</issn><eissn>1943-7692</eissn><coden>PLDIDE</coden><abstract>A rapid and simple reverse-transcription polymerase chain reaction (RT-PCR) method was developed for the detection of citrus tristeza virus (CTV) in three aphid species. Seven CTV isolates from a worldwide isolate collection were used for aphid acquisition feeding by three aphid species. These included the most efficient CTV vector, the brown citrus aphid, Toxoptera citricida; the melon aphid, Aphis gossypii; and the green peach aphid, Myzus persicae, a non-vector for CTV. A short procedure for nucleic acid extraction from single or groups of aphids was developed. Nucleic acid extracts from 1, 3, 5, and 10 aphids with acquisition-access periods of 24 and 48 h were reverse transcribed and amplified using primers for the coat protein gene of the Florida B3 (T-36) isolate of CTV. PCR-amplified fragments of approximately 670 bp were obtained from all the isolates tested and the amplified product from the aphids fed on citrus infected with isolate B3 was confirmed as the CTV coat protein gene by digesting with various restriction enzymes. This technique will be useful in investigations of CTV-vector-plant interactions and CTV epidemiology</abstract><cop>St. Paul, MN</cop><pub>American Phytopathological Society</pub><pmid>30861961</pmid><doi>10.1094/PDIS.1997.81.9.1066</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| source | EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection; American Phytopathological Society Journal Back Issues |
| subjects | APHIS GOSSYPII Biological and medical sciences CITRUS CITRUS TRISTEZA CLOSTEROVIRUS CLOSTEROVIRUS TRISTEZA DEL CITRUS CLOSTEROVIRUS TRISTEZA DU CITRUS COAT PROTEINS DISEASE VECTORS Fundamental and applied biological sciences. Psychology GENE Generalities. Techniques. Transmission, epidemiology, ecology. Antiviral substances, control GENES MICROBIAL PROTEINS MYZUS PERSICAE PCR Phytopathology. Animal pests. Plant and forest protection Plant viruses and viroids PROTEINAS MICROBIANAS PROTEINE MICROBIENNE REVERSE TRANSCRIPTION STRUCTURAL GENES TOXOPTERA CITRICIDUS TRANSCRIPCION INVERSA TRANSCRIPTION INVERSE VECTEUR DE MALADIE VECTORES VECTORS |
| title | Reverse-transcription polymerase chain reaction detection of citrus tristeza virus in aphids |
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