A novel visual-mixed-dye for LAMP and its application in the detection of foodborne pathogens

Loop-mediated isothermal amplification (LAMP) has been widely applied for the detection of foodborne pathogens. Obtaining a simple, accurate readout of LAMP reaction results is crucial. Herein, a visual-mixed-dye (VMD) containing calcein (precombined with MnCl2) and hydroxynaphthol blue (HNB) for LA...

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Veröffentlicht in:	Analytical biochemistry 2019-06, Vol.574, p.1-6
Hauptverfasser:	Pang, Bo, Yao, Shuo, Xu, Kun, Wang, Juan, Song, Xiuling, Mu, Ying, Zhao, Chao, Li, Juan
Format:	Artikel
Sprache:	eng
Schlagworte:	Coloring Agents - chemistry Fluoresceins - chemistry Food Microbiology Food safety Foodborne pathogens Gram-Negative Bacteria - isolation & purification Gram-Positive Bacteria - isolation & purification Loop-mediated isothermal amplification Mixed dye Naphthalenesulfonates - chemistry Nucleic Acid Amplification Techniques - methods Visual detection
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