Prevalence and Genotyping of Microsporidian Parasites in Dogs in Turkey: Zoonotic Concerns
Microsporidia are opportunistic pathogens that infect a wide range of invertebrates and vertebrates. To assess the potential role of dogs in the transmission of these zoonotic pathogens, a total of 282 fecal samples from dogs in the Central Anatolia Region of Turkey were analyzed by utilizing specie...
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| Veröffentlicht in: | The Journal of eukaryotic microbiology 2019-09, Vol.66 (5), p.771-777 |
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| container_title | The Journal of eukaryotic microbiology |
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| creator | Duzlu, Onder Yildirim, Alparslan Onder, Zuhal Ciloglu, Arif Yetismis, Gamze Inci, Abdullah |
| description | Microsporidia are opportunistic pathogens that infect a wide range of invertebrates and vertebrates. To assess the potential role of dogs in the transmission of these zoonotic pathogens, a total of 282 fecal samples from dogs in the Central Anatolia Region of Turkey were analyzed by utilizing species specific polymerase chain reaction for the four most frequent human microsporidia. Two microsporidia species were recognized in 41 samples (14.5%). Encephalitozoon intestinalis was detected in 35 samples (12.4%) and it was the most common microsporidium. The second microsporidium, E. cuniculi, was identified in six (2.1%) of the samples. Sequence analysis of the intergenic spacer of the ribosomal ribonucleic acid (RNA) internal transcribed spacer (ITS) gene revealed the presence of three E. intestinalis haplotypes closely associated with each other. No polymorphic region was found among the ITS sequences of E. cuniculi isolates and they were characterized as genotype III. This study provides the first data on the zoonotic microsporidia species from dogs in Turkey. |
| doi_str_mv | 10.1111/jeu.12725 |
| format | Article |
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To assess the potential role of dogs in the transmission of these zoonotic pathogens, a total of 282 fecal samples from dogs in the Central Anatolia Region of Turkey were analyzed by utilizing species specific polymerase chain reaction for the four most frequent human microsporidia. Two microsporidia species were recognized in 41 samples (14.5%). Encephalitozoon intestinalis was detected in 35 samples (12.4%) and it was the most common microsporidium. The second microsporidium, E. cuniculi, was identified in six (2.1%) of the samples. Sequence analysis of the intergenic spacer of the ribosomal ribonucleic acid (RNA) internal transcribed spacer (ITS) gene revealed the presence of three E. intestinalis haplotypes closely associated with each other. No polymorphic region was found among the ITS sequences of E. cuniculi isolates and they were characterized as genotype III. 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To assess the potential role of dogs in the transmission of these zoonotic pathogens, a total of 282 fecal samples from dogs in the Central Anatolia Region of Turkey were analyzed by utilizing species specific polymerase chain reaction for the four most frequent human microsporidia. Two microsporidia species were recognized in 41 samples (14.5%). Encephalitozoon intestinalis was detected in 35 samples (12.4%) and it was the most common microsporidium. The second microsporidium, E. cuniculi, was identified in six (2.1%) of the samples. Sequence analysis of the intergenic spacer of the ribosomal ribonucleic acid (RNA) internal transcribed spacer (ITS) gene revealed the presence of three E. intestinalis haplotypes closely associated with each other. No polymorphic region was found among the ITS sequences of E. cuniculi isolates and they were characterized as genotype III. This study provides the first data on the zoonotic microsporidia species from dogs in Turkey.</description><subject>Dog</subject><subject>Encephalitozoon cuniculi</subject><subject>Encephalitozoon intestinalis</subject><subject>Genotypes</subject><subject>Genotyping</subject><subject>Haplotypes</subject><subject>Invertebrates</subject><subject>Microsporidia</subject><subject>molecular characterization</subject><subject>Nucleic acids</subject><subject>Nucleotide sequence</subject><subject>Opportunist infection</subject><subject>Parasites</subject><subject>Pathogens</subject><subject>PCR</subject><subject>Polymerase chain reaction</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>rRNA</subject><subject>Spacer</subject><subject>Species</subject><subject>Zoonoses</subject><issn>1066-5234</issn><issn>1550-7408</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNp1kD1PwzAQhi0EonwN_AFkiQWGFNux88GGSikgEAztwmI5tlO5pHaxG1D-PaYBBiRuuRuee3T3AnCM0RDHuljodohJTtgW2MOMoSSnqNiOM8qyhJGUDsB-CAuEcEYw3gWDFBW0JDjbAy_PXr-LRlupobAKTrR1625l7By6Gj4a6V1YOW-UERY-Cy-CWesAjYXXbr7p09a_6u4SvjgXV42EIxdl3oZDsFOLJuij734AZjfj6eg2eXia3I2uHhKZspQlilaE1LWgskKZiocRqZjMRS4IyitaYMUyJOpS0wwLJFQqGSoIJayoVFVplB6As9678u6t1WHNlyZI3TTCatcGTnBRMppTmkX09A-6cK238TpOSJEjjNOSRuq8p76eD17XfOXNUviOY8S_AucxcL4JPLIn38a2Wmr1S_4kHIGLHvgwje7-N_H78axXfgL2s4mR</recordid><startdate>201909</startdate><enddate>201909</enddate><creator>Duzlu, Onder</creator><creator>Yildirim, Alparslan</creator><creator>Onder, Zuhal</creator><creator>Ciloglu, Arif</creator><creator>Yetismis, Gamze</creator><creator>Inci, Abdullah</creator><general>Wiley Subscription Services, Inc</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TN</scope><scope>8FD</scope><scope>F1W</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-6951-0901</orcidid></search><sort><creationdate>201909</creationdate><title>Prevalence and Genotyping of Microsporidian Parasites in Dogs in Turkey: Zoonotic Concerns</title><author>Duzlu, Onder ; Yildirim, Alparslan ; Onder, Zuhal ; Ciloglu, Arif ; Yetismis, Gamze ; Inci, Abdullah</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3535-d4b22ffa4cb06d0842cd5c7a7a207b481d560af9e461a0ad3c50824258bdbbe03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Dog</topic><topic>Encephalitozoon cuniculi</topic><topic>Encephalitozoon intestinalis</topic><topic>Genotypes</topic><topic>Genotyping</topic><topic>Haplotypes</topic><topic>Invertebrates</topic><topic>Microsporidia</topic><topic>molecular characterization</topic><topic>Nucleic acids</topic><topic>Nucleotide sequence</topic><topic>Opportunist infection</topic><topic>Parasites</topic><topic>Pathogens</topic><topic>PCR</topic><topic>Polymerase chain reaction</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><topic>rRNA</topic><topic>Spacer</topic><topic>Species</topic><topic>Zoonoses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Duzlu, Onder</creatorcontrib><creatorcontrib>Yildirim, Alparslan</creatorcontrib><creatorcontrib>Onder, Zuhal</creatorcontrib><creatorcontrib>Ciloglu, Arif</creatorcontrib><creatorcontrib>Yetismis, Gamze</creatorcontrib><creatorcontrib>Inci, Abdullah</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Oceanic Abstracts</collection><collection>Technology Research Database</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of eukaryotic microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Duzlu, Onder</au><au>Yildirim, Alparslan</au><au>Onder, Zuhal</au><au>Ciloglu, Arif</au><au>Yetismis, Gamze</au><au>Inci, Abdullah</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Prevalence and Genotyping of Microsporidian Parasites in Dogs in Turkey: Zoonotic Concerns</atitle><jtitle>The Journal of eukaryotic microbiology</jtitle><addtitle>J Eukaryot Microbiol</addtitle><date>2019-09</date><risdate>2019</risdate><volume>66</volume><issue>5</issue><spage>771</spage><epage>777</epage><pages>771-777</pages><issn>1066-5234</issn><eissn>1550-7408</eissn><abstract>Microsporidia are opportunistic pathogens that infect a wide range of invertebrates and vertebrates. To assess the potential role of dogs in the transmission of these zoonotic pathogens, a total of 282 fecal samples from dogs in the Central Anatolia Region of Turkey were analyzed by utilizing species specific polymerase chain reaction for the four most frequent human microsporidia. Two microsporidia species were recognized in 41 samples (14.5%). Encephalitozoon intestinalis was detected in 35 samples (12.4%) and it was the most common microsporidium. The second microsporidium, E. cuniculi, was identified in six (2.1%) of the samples. Sequence analysis of the intergenic spacer of the ribosomal ribonucleic acid (RNA) internal transcribed spacer (ITS) gene revealed the presence of three E. intestinalis haplotypes closely associated with each other. No polymorphic region was found among the ITS sequences of E. cuniculi isolates and they were characterized as genotype III. 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| source | Wiley Online Library Journals Frontfile Complete |
| subjects | Dog Encephalitozoon cuniculi Encephalitozoon intestinalis Genotypes Genotyping Haplotypes Invertebrates Microsporidia molecular characterization Nucleic acids Nucleotide sequence Opportunist infection Parasites Pathogens PCR Polymerase chain reaction Ribonucleic acid RNA rRNA Spacer Species Zoonoses |
| title | Prevalence and Genotyping of Microsporidian Parasites in Dogs in Turkey: Zoonotic Concerns |
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