Development of a Highly Sensitive Direct Competitive Fluorescence Enzyme Immunoassay Based on a Nanobody–Alkaline Phosphatase Fusion Protein for Detection of 3‑Phenoxybenzoic Acid in Urine

3-Phenoxybenzoic acid (3-PBA) is a human urinary metabolite of many pyrethroid insecticides and can be used as a biomarker to monitor human exposure to these pesticides. A rapid and sensitive direct competitive fluorescence enzyme immunoassay (dc-FEIA) for detecting 3-PBA on the basis of a nanobody...

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Veröffentlicht in:	Journal of agricultural and food chemistry 2018-10, Vol.66 (43), p.11284-11290
Hauptverfasser:	Huo, Jingqian, Li, Zhenfeng, Wan, Debin, Li, Dongyang, Qi, Meng, Barnych, Bogdan, Vasylieva, Natalia, Zhang, Jinlin, Hammock, Bruce D
Format:	Artikel
Sprache:	eng
Schlagworte:	Alkaline Phosphatase - chemistry Benzoates - urine biomarkers Biomarkers - urine Enzyme-Linked Immunosorbent Assay enzymes fluorescence Humans Immunoenzyme Techniques inhibitory concentration 50 liquid chromatography mass spectrometry metabolites Pesticide Residues - urine Pyrethrins - urine pyrethroid insecticides Recombinant Fusion Proteins - chemistry Single-Domain Antibodies - chemistry urine
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