Modified Procedure to Inoculate Micro-ID

The accuracy of a miniaturized Enterobacteriaceae system (Micro-ID) was evaluated with 10 different genera grown on 11 selective plating media. Whenever growth occurred, the correct identification was made. In addition, 12 serotypes of Salmonella and 22 other strains of Enterobacteriaceae were grown...

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Veröffentlicht in:Journal of food protection 1980-10, Vol.43 (10), p.774-777
Hauptverfasser: Cox, N A, Bailey, J S, Thomson, J E, Carson, M O, Posey, D A, Rivera, E
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container_end_page 777
container_issue 10
container_start_page 774
container_title Journal of food protection
container_volume 43
creator Cox, N A
Bailey, J S
Thomson, J E
Carson, M O
Posey, D A
Rivera, E
description The accuracy of a miniaturized Enterobacteriaceae system (Micro-ID) was evaluated with 10 different genera grown on 11 selective plating media. Whenever growth occurred, the correct identification was made. In addition, 12 serotypes of Salmonella and 22 other strains of Enterobacteriaceae were grown on the two most common selective media used by food microbiologists for isolation of Salmonella [Brilliant Green Sulfa Agar (BGS) and Bismuth Sulfite Agar (BiS)]. The correct identification was made in all instances. The manufacturer of Micro-ID originally recommended that several colonies be picked for inoculation of the system. We modified the inoculation procedure so that only one colony was selected from a plate; this minimized the chances of having a mixed-culture inoculum. With this procedure, all 21 Enterobacteriaceae tested were correctly identified.
doi_str_mv 10.4315/0362-028X-43.10.774
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title Modified Procedure to Inoculate Micro-ID
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