Staining of endothelial cells does not change the result of cell density
To determine the corneal endothelial cell density human donor corneas are usually placed in hypotonic balanced salt solution for unstained cell counting. Experimental studies often stain the corneal endothelium to highlight cells and cell borders and simplify counting. However, staining normally ter...
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| Veröffentlicht in: | Cell and tissue banking 2019-06, Vol.20 (2), p.327-328 |
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| creator | Wenzel, Daniel A. Kunzmann, Berenike C. Spitzer, Martin S. Schultheiss, Maximilian |
| description | To determine the corneal endothelial cell density human donor corneas are usually placed in hypotonic balanced salt solution for unstained cell counting. Experimental studies often stain the corneal endothelium to highlight cells and cell borders and simplify counting. However, staining normally terminates the experiments. Up to date, there is no publication comparing endothelial cell counts before and after staining. This study compared the counting results of unstained (hypotonic balanced salt solution) and stained (trypan blue & alizarin red S) assessment of the corneal endothelial cell density. No evidence for significantly differing cell counts was found between unstained and stained analysis of the corneal endothelial cell density. Therefore, we consider unstained counting non-inferior to stained counting. |
| doi_str_mv | 10.1007/s10561-019-09759-6 |
| format | Article |
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Experimental studies often stain the corneal endothelium to highlight cells and cell borders and simplify counting. However, staining normally terminates the experiments. Up to date, there is no publication comparing endothelial cell counts before and after staining. This study compared the counting results of unstained (hypotonic balanced salt solution) and stained (trypan blue & alizarin red S) assessment of the corneal endothelial cell density. No evidence for significantly differing cell counts was found between unstained and stained analysis of the corneal endothelial cell density. Therefore, we consider unstained counting non-inferior to stained counting.</description><identifier>ISSN: 1389-9333</identifier><identifier>EISSN: 1573-6814</identifier><identifier>DOI: 10.1007/s10561-019-09759-6</identifier><identifier>PMID: 30810847</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Alizarin ; Anthraquinones - pharmacology ; Biomedical and Life Sciences ; Biomedicine ; Cell Biology ; Cell Count - methods ; Cell density ; Cell Survival - drug effects ; Coloring Agents - pharmacology ; Cornea ; Counting ; Density ; Endothelial cells ; Endothelium ; Endothelium, Corneal - cytology ; Humans ; Life Sciences ; Saline solutions ; Short Communication ; Staining ; Transplant Surgery ; Trypan Blue - pharmacology</subject><ispartof>Cell and tissue banking, 2019-06, Vol.20 (2), p.327-328</ispartof><rights>Springer Nature B.V. 2019</rights><rights>Cell and Tissue Banking is a copyright of Springer, (2019). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c375t-a910802479977e69c00acba974b043c65a6ecb0133130f27a519db0450c8a0813</citedby><cites>FETCH-LOGICAL-c375t-a910802479977e69c00acba974b043c65a6ecb0133130f27a519db0450c8a0813</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10561-019-09759-6$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10561-019-09759-6$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27915,27916,41479,42548,51310</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30810847$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wenzel, Daniel A.</creatorcontrib><creatorcontrib>Kunzmann, Berenike C.</creatorcontrib><creatorcontrib>Spitzer, Martin S.</creatorcontrib><creatorcontrib>Schultheiss, Maximilian</creatorcontrib><title>Staining of endothelial cells does not change the result of cell density</title><title>Cell and tissue banking</title><addtitle>Cell Tissue Bank</addtitle><addtitle>Cell Tissue Bank</addtitle><description>To determine the corneal endothelial cell density human donor corneas are usually placed in hypotonic balanced salt solution for unstained cell counting. Experimental studies often stain the corneal endothelium to highlight cells and cell borders and simplify counting. However, staining normally terminates the experiments. Up to date, there is no publication comparing endothelial cell counts before and after staining. This study compared the counting results of unstained (hypotonic balanced salt solution) and stained (trypan blue & alizarin red S) assessment of the corneal endothelial cell density. No evidence for significantly differing cell counts was found between unstained and stained analysis of the corneal endothelial cell density. Therefore, we consider unstained counting non-inferior to stained counting.</description><subject>Alizarin</subject><subject>Anthraquinones - pharmacology</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Cell Biology</subject><subject>Cell Count - methods</subject><subject>Cell density</subject><subject>Cell Survival - drug effects</subject><subject>Coloring Agents - pharmacology</subject><subject>Cornea</subject><subject>Counting</subject><subject>Density</subject><subject>Endothelial cells</subject><subject>Endothelium</subject><subject>Endothelium, Corneal - cytology</subject><subject>Humans</subject><subject>Life Sciences</subject><subject>Saline solutions</subject><subject>Short Communication</subject><subject>Staining</subject><subject>Transplant Surgery</subject><subject>Trypan Blue - pharmacology</subject><issn>1389-9333</issn><issn>1573-6814</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kLFOwzAURS0EoqXwAwzIEguL4dlO7HhEFVCkSgzAbDmO06ZK4xInQ_8ehxSQGJhs6Z1339VB6JLCLQWQd4FCKigBqggomSoijtCUppITkdHkOP55pojinE_QWQgbAAaS8VM04ZBRyBI5RYvXzlRN1aywL7FrCt-tXV2ZGltX1wEX3gXc-A7btWlWDscpbl3o627gBwYXrglVtz9HJ6Wpg7s4vDP0_vjwNl-Q5cvT8_x-SSyXaUeMioeBJVIpKZ1QFsDY3CiZ5JBwK1IjnM2Bck45lEyalKoijlKwmYmt-QzdjLm71n_0LnR6W4WhiGmc74NmNBOC8YxmEb3-g2583zax3UiBYjBQbKRs60NoXal3bbU17V5T0INnPXrW0bP-8qxFXLo6RPf51hU_K99iI8BHIMRRNNf-3v4n9hOfioYP</recordid><startdate>20190601</startdate><enddate>20190601</enddate><creator>Wenzel, Daniel A.</creator><creator>Kunzmann, Berenike C.</creator><creator>Spitzer, Martin S.</creator><creator>Schultheiss, Maximilian</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>20190601</creationdate><title>Staining of endothelial cells does not change the result of cell density</title><author>Wenzel, Daniel A. ; 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Experimental studies often stain the corneal endothelium to highlight cells and cell borders and simplify counting. However, staining normally terminates the experiments. Up to date, there is no publication comparing endothelial cell counts before and after staining. This study compared the counting results of unstained (hypotonic balanced salt solution) and stained (trypan blue & alizarin red S) assessment of the corneal endothelial cell density. No evidence for significantly differing cell counts was found between unstained and stained analysis of the corneal endothelial cell density. Therefore, we consider unstained counting non-inferior to stained counting.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>30810847</pmid><doi>10.1007/s10561-019-09759-6</doi><tpages>2</tpages></addata></record> |
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| subjects | Alizarin Anthraquinones - pharmacology Biomedical and Life Sciences Biomedicine Cell Biology Cell Count - methods Cell density Cell Survival - drug effects Coloring Agents - pharmacology Cornea Counting Density Endothelial cells Endothelium Endothelium, Corneal - cytology Humans Life Sciences Saline solutions Short Communication Staining Transplant Surgery Trypan Blue - pharmacology |
| title | Staining of endothelial cells does not change the result of cell density |
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