Supplementation of organ culture medium with dextran is not required in pre-stripped human donor tissue for DMEK surgery
To assess corneal endothelial cell (CEC) quantity and quality in eye-bank prepared lamellar grafts for Descemet Membrane Endothelial Keratoplasty (DMEK) from organ cultured donor corneas that have not undergone de-swelling by media supplementation with dextran. Prior to graft preparation, corneas th...
Gespeichert in:
| Veröffentlicht in: | Cell and tissue banking 2019-06, Vol.20 (2), p.193-200 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 200 |
|---|---|
| container_issue | 2 |
| container_start_page | 193 |
| container_title | Cell and tissue banking |
| container_volume | 20 |
| creator | Salla, Sabine Kruse, Friedrich E. Walter, Peter Menzel-Severing, Johannes |
| description | To assess corneal endothelial cell (CEC) quantity and quality in eye-bank prepared lamellar grafts for Descemet Membrane Endothelial Keratoplasty (DMEK) from organ cultured donor corneas that have not undergone de-swelling by media supplementation with dextran. Prior to graft preparation, corneas that had not undergone de-swelling (n = 30) were placed into fresh storage medium without dextran (KMI). Corneas in the control group (n = 30) were placed in dehydration medium containing 5% dextran (KMII). Subtotal stripping of Descemet’s membrane (DM) was performed manually. Following graft preparation, 10 corneas of each group were cultured further in their respective medium for 24 h, 72 h, or 120 h, respectively. Before and after DM stripping, as well as at the end of culture, CEC numbers were obtained. At the end of culture, CEC morphology was graded using a scoring system and CEC metabolism was assessed by detection of adenosine triphosphate. At 24 h after DM stripping, mean CEC counts (in cells/mm
2
) were 2204 in corneas stored in KMII, and 2391 in corneas stored in KMI (
p
= 0.003). This corresponds to a mean relative CEC loss of 12.4% with dextran versus 9.7% without dextran (
p
= 0.04). At 72 h, CEC counts were 1946 in KMII, and 2289 in KMI (
p
= 0.004). This corresponds to CEC loss of 23% with dextran versus 14% without dextran (
p
= 0.009). At 120 h, CEC counts were 2047 in KMII, and 2230 in KMI (
p
= 0.14). This corresponds to CEC loss of 22.7% with dextran versus 17.2% without dextran (
p
= 0.14). Also, at 120 h after DM stripping, 6/10 corneas fell below a threshold of 2000 cells/mm
2
if stored in medium containing dextran, versus 1/9 corneas if stored without dextran (
p
= 0.003). Morphological assessment of CEC quality revealed equal scores for cell polymorphism (median = 1), granulation (median = 0) and segmentation (median = 1) in all groups. Lower ATP/protein ratios were observed in corneas stored in medium without dextran at 24 h (
p
|
| doi_str_mv | 10.1007/s10561-019-09757-8 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2185877655</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2185877655</sourcerecordid><originalsourceid>FETCH-LOGICAL-c419t-cfbcd3cdda64f7a03975c3d8d10294cbce6c0749616d3ac6bfc59c79b1b8c53c3</originalsourceid><addsrcrecordid>eNp9kctO3TAQhq2qiPsLsKgsdcPGxY6T2F5W3FoVxAJYW4ntnGOU2MEXcc7bYzhQJBasZkbzzT_2_AAcEfyLYMxOIsFNSxAmAmHBGob4N7BLGkZRy0n9veSUCyQopTtgL8YHjCvMKroNdihmgteY7YLVbZ7n0UzGpS5Z76AfoA-LzkGVx5SDgZPRNk_wyaYl1GaVQunZCJ1PMJjHbIPR0Do4B4NiCnaeS73MU6G0dz7AZGPMBg4lPbs-_wdjDgsT1gdga-jGaA7f4j64vzi_O_2Drm4u_57-vkKqJiIhNfRKU6V119YD6zAtH1VUc01wJWrVK9MqzGrRklbTTrX9oBqhmOhJz1VDFd0HxxvdOfjHbGKSk43KjGPnjM9RVoQ3nLG2aQr68xP64HNw5XWvFKkFEaJQ1YZSwccYzCDnYKcurCXB8sUXufFFFl_kqy-Sl6Efb9K5Lwf9P_JuRAHoBoil5cqBPnZ_IfsMMCia5Q</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2185149199</pqid></control><display><type>article</type><title>Supplementation of organ culture medium with dextran is not required in pre-stripped human donor tissue for DMEK surgery</title><source>MEDLINE</source><source>SpringerLink Journals - AutoHoldings</source><creator>Salla, Sabine ; Kruse, Friedrich E. ; Walter, Peter ; Menzel-Severing, Johannes</creator><creatorcontrib>Salla, Sabine ; Kruse, Friedrich E. ; Walter, Peter ; Menzel-Severing, Johannes</creatorcontrib><description>To assess corneal endothelial cell (CEC) quantity and quality in eye-bank prepared lamellar grafts for Descemet Membrane Endothelial Keratoplasty (DMEK) from organ cultured donor corneas that have not undergone de-swelling by media supplementation with dextran. Prior to graft preparation, corneas that had not undergone de-swelling (n = 30) were placed into fresh storage medium without dextran (KMI). Corneas in the control group (n = 30) were placed in dehydration medium containing 5% dextran (KMII). Subtotal stripping of Descemet’s membrane (DM) was performed manually. Following graft preparation, 10 corneas of each group were cultured further in their respective medium for 24 h, 72 h, or 120 h, respectively. Before and after DM stripping, as well as at the end of culture, CEC numbers were obtained. At the end of culture, CEC morphology was graded using a scoring system and CEC metabolism was assessed by detection of adenosine triphosphate. At 24 h after DM stripping, mean CEC counts (in cells/mm
2
) were 2204 in corneas stored in KMII, and 2391 in corneas stored in KMI (
p
= 0.003). This corresponds to a mean relative CEC loss of 12.4% with dextran versus 9.7% without dextran (
p
= 0.04). At 72 h, CEC counts were 1946 in KMII, and 2289 in KMI (
p
= 0.004). This corresponds to CEC loss of 23% with dextran versus 14% without dextran (
p
= 0.009). At 120 h, CEC counts were 2047 in KMII, and 2230 in KMI (
p
= 0.14). This corresponds to CEC loss of 22.7% with dextran versus 17.2% without dextran (
p
= 0.14). Also, at 120 h after DM stripping, 6/10 corneas fell below a threshold of 2000 cells/mm
2
if stored in medium containing dextran, versus 1/9 corneas if stored without dextran (
p
= 0.003). Morphological assessment of CEC quality revealed equal scores for cell polymorphism (median = 1), granulation (median = 0) and segmentation (median = 1) in all groups. Lower ATP/protein ratios were observed in corneas stored in medium without dextran at 24 h (
p
< 0.001), 72 h (
p
< 0.001), and 120 h (
p
= 0.02). Abandoning the use of dextran in corneas destined for DMEK surgery leads to increased CEC counts and thereby serves to reduce tissue loss.</description><identifier>ISSN: 1389-9333</identifier><identifier>EISSN: 1573-6814</identifier><identifier>DOI: 10.1007/s10561-019-09757-8</identifier><identifier>PMID: 30798407</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Adenosine triphosphate ; ATP ; Biomedical and Life Sciences ; Biomedicine ; Cell Biology ; Cell culture ; Cornea ; Cornea - cytology ; Cornea - surgery ; Corneal transplantation ; Culture media ; Culture Media - chemistry ; Dehydration ; Descemet Stripping Endothelial Keratoplasty - methods ; Dextran ; Dextrans - pharmacology ; Endothelial cells ; Endothelial Cells - cytology ; Eye surgery ; Grafting ; Grafts ; Granulation ; Humans ; Life Sciences ; Metabolism ; Morphology ; Ophthalmology ; Organ culture ; Organ Culture Techniques - methods ; Polymorphism ; Proteins ; Segmentation ; Supplements ; Surgery ; Tissue Banks ; Tissues ; Transplant Surgery ; Transplants & implants</subject><ispartof>Cell and tissue banking, 2019-06, Vol.20 (2), p.193-200</ispartof><rights>Springer Nature B.V. 2019</rights><rights>Cell and Tissue Banking is a copyright of Springer, (2019). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c419t-cfbcd3cdda64f7a03975c3d8d10294cbce6c0749616d3ac6bfc59c79b1b8c53c3</citedby><cites>FETCH-LOGICAL-c419t-cfbcd3cdda64f7a03975c3d8d10294cbce6c0749616d3ac6bfc59c79b1b8c53c3</cites><orcidid>0000-0001-8194-1037</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10561-019-09757-8$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10561-019-09757-8$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,777,781,27905,27906,41469,42538,51300</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30798407$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Salla, Sabine</creatorcontrib><creatorcontrib>Kruse, Friedrich E.</creatorcontrib><creatorcontrib>Walter, Peter</creatorcontrib><creatorcontrib>Menzel-Severing, Johannes</creatorcontrib><title>Supplementation of organ culture medium with dextran is not required in pre-stripped human donor tissue for DMEK surgery</title><title>Cell and tissue banking</title><addtitle>Cell Tissue Bank</addtitle><addtitle>Cell Tissue Bank</addtitle><description>To assess corneal endothelial cell (CEC) quantity and quality in eye-bank prepared lamellar grafts for Descemet Membrane Endothelial Keratoplasty (DMEK) from organ cultured donor corneas that have not undergone de-swelling by media supplementation with dextran. Prior to graft preparation, corneas that had not undergone de-swelling (n = 30) were placed into fresh storage medium without dextran (KMI). Corneas in the control group (n = 30) were placed in dehydration medium containing 5% dextran (KMII). Subtotal stripping of Descemet’s membrane (DM) was performed manually. Following graft preparation, 10 corneas of each group were cultured further in their respective medium for 24 h, 72 h, or 120 h, respectively. Before and after DM stripping, as well as at the end of culture, CEC numbers were obtained. At the end of culture, CEC morphology was graded using a scoring system and CEC metabolism was assessed by detection of adenosine triphosphate. At 24 h after DM stripping, mean CEC counts (in cells/mm
2
) were 2204 in corneas stored in KMII, and 2391 in corneas stored in KMI (
p
= 0.003). This corresponds to a mean relative CEC loss of 12.4% with dextran versus 9.7% without dextran (
p
= 0.04). At 72 h, CEC counts were 1946 in KMII, and 2289 in KMI (
p
= 0.004). This corresponds to CEC loss of 23% with dextran versus 14% without dextran (
p
= 0.009). At 120 h, CEC counts were 2047 in KMII, and 2230 in KMI (
p
= 0.14). This corresponds to CEC loss of 22.7% with dextran versus 17.2% without dextran (
p
= 0.14). Also, at 120 h after DM stripping, 6/10 corneas fell below a threshold of 2000 cells/mm
2
if stored in medium containing dextran, versus 1/9 corneas if stored without dextran (
p
= 0.003). Morphological assessment of CEC quality revealed equal scores for cell polymorphism (median = 1), granulation (median = 0) and segmentation (median = 1) in all groups. Lower ATP/protein ratios were observed in corneas stored in medium without dextran at 24 h (
p
< 0.001), 72 h (
p
< 0.001), and 120 h (
p
= 0.02). Abandoning the use of dextran in corneas destined for DMEK surgery leads to increased CEC counts and thereby serves to reduce tissue loss.</description><subject>Adenosine triphosphate</subject><subject>ATP</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Cell Biology</subject><subject>Cell culture</subject><subject>Cornea</subject><subject>Cornea - cytology</subject><subject>Cornea - surgery</subject><subject>Corneal transplantation</subject><subject>Culture media</subject><subject>Culture Media - chemistry</subject><subject>Dehydration</subject><subject>Descemet Stripping Endothelial Keratoplasty - methods</subject><subject>Dextran</subject><subject>Dextrans - pharmacology</subject><subject>Endothelial cells</subject><subject>Endothelial Cells - cytology</subject><subject>Eye surgery</subject><subject>Grafting</subject><subject>Grafts</subject><subject>Granulation</subject><subject>Humans</subject><subject>Life Sciences</subject><subject>Metabolism</subject><subject>Morphology</subject><subject>Ophthalmology</subject><subject>Organ culture</subject><subject>Organ Culture Techniques - methods</subject><subject>Polymorphism</subject><subject>Proteins</subject><subject>Segmentation</subject><subject>Supplements</subject><subject>Surgery</subject><subject>Tissue Banks</subject><subject>Tissues</subject><subject>Transplant Surgery</subject><subject>Transplants & implants</subject><issn>1389-9333</issn><issn>1573-6814</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kctO3TAQhq2qiPsLsKgsdcPGxY6T2F5W3FoVxAJYW4ntnGOU2MEXcc7bYzhQJBasZkbzzT_2_AAcEfyLYMxOIsFNSxAmAmHBGob4N7BLGkZRy0n9veSUCyQopTtgL8YHjCvMKroNdihmgteY7YLVbZ7n0UzGpS5Z76AfoA-LzkGVx5SDgZPRNk_wyaYl1GaVQunZCJ1PMJjHbIPR0Do4B4NiCnaeS73MU6G0dz7AZGPMBg4lPbs-_wdjDgsT1gdga-jGaA7f4j64vzi_O_2Drm4u_57-vkKqJiIhNfRKU6V119YD6zAtH1VUc01wJWrVK9MqzGrRklbTTrX9oBqhmOhJz1VDFd0HxxvdOfjHbGKSk43KjGPnjM9RVoQ3nLG2aQr68xP64HNw5XWvFKkFEaJQ1YZSwccYzCDnYKcurCXB8sUXufFFFl_kqy-Sl6Efb9K5Lwf9P_JuRAHoBoil5cqBPnZ_IfsMMCia5Q</recordid><startdate>20190601</startdate><enddate>20190601</enddate><creator>Salla, Sabine</creator><creator>Kruse, Friedrich E.</creator><creator>Walter, Peter</creator><creator>Menzel-Severing, Johannes</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-8194-1037</orcidid></search><sort><creationdate>20190601</creationdate><title>Supplementation of organ culture medium with dextran is not required in pre-stripped human donor tissue for DMEK surgery</title><author>Salla, Sabine ; Kruse, Friedrich E. ; Walter, Peter ; Menzel-Severing, Johannes</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c419t-cfbcd3cdda64f7a03975c3d8d10294cbce6c0749616d3ac6bfc59c79b1b8c53c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Adenosine triphosphate</topic><topic>ATP</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Cell Biology</topic><topic>Cell culture</topic><topic>Cornea</topic><topic>Cornea - cytology</topic><topic>Cornea - surgery</topic><topic>Corneal transplantation</topic><topic>Culture media</topic><topic>Culture Media - chemistry</topic><topic>Dehydration</topic><topic>Descemet Stripping Endothelial Keratoplasty - methods</topic><topic>Dextran</topic><topic>Dextrans - pharmacology</topic><topic>Endothelial cells</topic><topic>Endothelial Cells - cytology</topic><topic>Eye surgery</topic><topic>Grafting</topic><topic>Grafts</topic><topic>Granulation</topic><topic>Humans</topic><topic>Life Sciences</topic><topic>Metabolism</topic><topic>Morphology</topic><topic>Ophthalmology</topic><topic>Organ culture</topic><topic>Organ Culture Techniques - methods</topic><topic>Polymorphism</topic><topic>Proteins</topic><topic>Segmentation</topic><topic>Supplements</topic><topic>Surgery</topic><topic>Tissue Banks</topic><topic>Tissues</topic><topic>Transplant Surgery</topic><topic>Transplants & implants</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Salla, Sabine</creatorcontrib><creatorcontrib>Kruse, Friedrich E.</creatorcontrib><creatorcontrib>Walter, Peter</creatorcontrib><creatorcontrib>Menzel-Severing, Johannes</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nursing & Allied Health Database</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>Cell and tissue banking</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Salla, Sabine</au><au>Kruse, Friedrich E.</au><au>Walter, Peter</au><au>Menzel-Severing, Johannes</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Supplementation of organ culture medium with dextran is not required in pre-stripped human donor tissue for DMEK surgery</atitle><jtitle>Cell and tissue banking</jtitle><stitle>Cell Tissue Bank</stitle><addtitle>Cell Tissue Bank</addtitle><date>2019-06-01</date><risdate>2019</risdate><volume>20</volume><issue>2</issue><spage>193</spage><epage>200</epage><pages>193-200</pages><issn>1389-9333</issn><eissn>1573-6814</eissn><abstract>To assess corneal endothelial cell (CEC) quantity and quality in eye-bank prepared lamellar grafts for Descemet Membrane Endothelial Keratoplasty (DMEK) from organ cultured donor corneas that have not undergone de-swelling by media supplementation with dextran. Prior to graft preparation, corneas that had not undergone de-swelling (n = 30) were placed into fresh storage medium without dextran (KMI). Corneas in the control group (n = 30) were placed in dehydration medium containing 5% dextran (KMII). Subtotal stripping of Descemet’s membrane (DM) was performed manually. Following graft preparation, 10 corneas of each group were cultured further in their respective medium for 24 h, 72 h, or 120 h, respectively. Before and after DM stripping, as well as at the end of culture, CEC numbers were obtained. At the end of culture, CEC morphology was graded using a scoring system and CEC metabolism was assessed by detection of adenosine triphosphate. At 24 h after DM stripping, mean CEC counts (in cells/mm
2
) were 2204 in corneas stored in KMII, and 2391 in corneas stored in KMI (
p
= 0.003). This corresponds to a mean relative CEC loss of 12.4% with dextran versus 9.7% without dextran (
p
= 0.04). At 72 h, CEC counts were 1946 in KMII, and 2289 in KMI (
p
= 0.004). This corresponds to CEC loss of 23% with dextran versus 14% without dextran (
p
= 0.009). At 120 h, CEC counts were 2047 in KMII, and 2230 in KMI (
p
= 0.14). This corresponds to CEC loss of 22.7% with dextran versus 17.2% without dextran (
p
= 0.14). Also, at 120 h after DM stripping, 6/10 corneas fell below a threshold of 2000 cells/mm
2
if stored in medium containing dextran, versus 1/9 corneas if stored without dextran (
p
= 0.003). Morphological assessment of CEC quality revealed equal scores for cell polymorphism (median = 1), granulation (median = 0) and segmentation (median = 1) in all groups. Lower ATP/protein ratios were observed in corneas stored in medium without dextran at 24 h (
p
< 0.001), 72 h (
p
< 0.001), and 120 h (
p
= 0.02). Abandoning the use of dextran in corneas destined for DMEK surgery leads to increased CEC counts and thereby serves to reduce tissue loss.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>30798407</pmid><doi>10.1007/s10561-019-09757-8</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0001-8194-1037</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1389-9333 |
| ispartof | Cell and tissue banking, 2019-06, Vol.20 (2), p.193-200 |
| issn | 1389-9333 1573-6814 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2185877655 |
| source | MEDLINE; SpringerLink Journals - AutoHoldings |
| subjects | Adenosine triphosphate ATP Biomedical and Life Sciences Biomedicine Cell Biology Cell culture Cornea Cornea - cytology Cornea - surgery Corneal transplantation Culture media Culture Media - chemistry Dehydration Descemet Stripping Endothelial Keratoplasty - methods Dextran Dextrans - pharmacology Endothelial cells Endothelial Cells - cytology Eye surgery Grafting Grafts Granulation Humans Life Sciences Metabolism Morphology Ophthalmology Organ culture Organ Culture Techniques - methods Polymorphism Proteins Segmentation Supplements Surgery Tissue Banks Tissues Transplant Surgery Transplants & implants |
| title | Supplementation of organ culture medium with dextran is not required in pre-stripped human donor tissue for DMEK surgery |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-18T07%3A06%3A16IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Supplementation%20of%20organ%20culture%20medium%20with%20dextran%20is%20not%20required%20in%20pre-stripped%20human%20donor%20tissue%20for%20DMEK%20surgery&rft.jtitle=Cell%20and%20tissue%20banking&rft.au=Salla,%20Sabine&rft.date=2019-06-01&rft.volume=20&rft.issue=2&rft.spage=193&rft.epage=200&rft.pages=193-200&rft.issn=1389-9333&rft.eissn=1573-6814&rft_id=info:doi/10.1007/s10561-019-09757-8&rft_dat=%3Cproquest_cross%3E2185877655%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2185149199&rft_id=info:pmid/30798407&rfr_iscdi=true |