A new lysosome-targetable fluorescent probe with a large Stokes shift for detection of endogenous hydrogen polysulfides in living cells
Hydrogen polysulfides (H2Sn, n > 1) influence a variety of important biological functions and activities associated with hydrogen sulfide (H2S). The development of probes for rapid, selective, and sensitive detection of H2Sn still remains a great challenge. Lysosome plays crucial roles in various...
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| Veröffentlicht in: | Analytica chimica acta 2019-05, Vol.1056, p.117-124 |
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| creator | Ren, Yanshen Zhang, Lulu Zhou, Ziyan Luo, Yanjun Wang, Suilou Yuan, Shengtao Gu, Yueqing Xu, Yungen Zha, Xiaoming |
| description | Hydrogen polysulfides (H2Sn, n > 1) influence a variety of important biological functions and activities associated with hydrogen sulfide (H2S). The development of probes for rapid, selective, and sensitive detection of H2Sn still remains a great challenge. Lysosome plays crucial roles in various physiological processes among living cells, which arouse high interest in detecting endogenous lysosome-targetable H2Sn. To the best of our knowledge, there is no lysosome-targetable probe for H2Sn has been reported. In this work, a new lysosome-targetable probe NIPYNF, based on the scaffold of imidazo [1,5-]pyridine, with a large Stokes shift (215 nm), low detection limit (84 nM), fast response time (6 min) and low cytotoxicity was designed and synthesized. Furthermore, NIPYNF was successfully applied into the cell imaging for detection of endogenous lysosome-targetable H2Sn.
[Display omitted]
•A novel lysosome-targetable probe NIPYNF was reported.•NIPYNF could discriminate H2Sn over other analytes with high sensitivity, a low detection limit and a large Stokes shift.•NIPYNF could detect the endogenous H2Sn in lysosome of living cells. |
| doi_str_mv | 10.1016/j.aca.2018.12.051 |
| format | Article |
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[Display omitted]
•A novel lysosome-targetable probe NIPYNF was reported.•NIPYNF could discriminate H2Sn over other analytes with high sensitivity, a low detection limit and a large Stokes shift.•NIPYNF could detect the endogenous H2Sn in lysosome of living cells.</description><identifier>ISSN: 0003-2670</identifier><identifier>EISSN: 1873-4324</identifier><identifier>DOI: 10.1016/j.aca.2018.12.051</identifier><identifier>PMID: 30797452</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>A549 Cells ; Cell imaging ; Cell Survival ; Cells (biology) ; Cytotoxicity ; Fluorescent Dyes - metabolism ; Fluorescent indicators ; Fluorescent probe ; Humans ; Hydrogen ; Hydrogen polysulfides ; Hydrogen sulfide ; Hydrogen Sulfide - chemistry ; Hydrogen Sulfide - metabolism ; Hydrogen-Ion Concentration ; Imidazo[1,5-]pyridine ; Lysosome ; Lysosomes - metabolism ; Optical Imaging ; Polysulfides ; Pyridines ; Response time ; Spectrometry, Fluorescence ; Toxicity</subject><ispartof>Analytica chimica acta, 2019-05, Vol.1056, p.117-124</ispartof><rights>2019 Elsevier B.V.</rights><rights>Copyright © 2019 Elsevier B.V. All rights reserved.</rights><rights>Copyright Elsevier BV May 16, 2019</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c381t-fc148f61981f648e14b1209f67997f25474161f88e0b5d08407cf4764474754f3</citedby><cites>FETCH-LOGICAL-c381t-fc148f61981f648e14b1209f67997f25474161f88e0b5d08407cf4764474754f3</cites><orcidid>0000-0003-1430-4067 ; 0000-0002-9506-2102</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.aca.2018.12.051$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30797452$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ren, Yanshen</creatorcontrib><creatorcontrib>Zhang, Lulu</creatorcontrib><creatorcontrib>Zhou, Ziyan</creatorcontrib><creatorcontrib>Luo, Yanjun</creatorcontrib><creatorcontrib>Wang, Suilou</creatorcontrib><creatorcontrib>Yuan, Shengtao</creatorcontrib><creatorcontrib>Gu, Yueqing</creatorcontrib><creatorcontrib>Xu, Yungen</creatorcontrib><creatorcontrib>Zha, Xiaoming</creatorcontrib><title>A new lysosome-targetable fluorescent probe with a large Stokes shift for detection of endogenous hydrogen polysulfides in living cells</title><title>Analytica chimica acta</title><addtitle>Anal Chim Acta</addtitle><description>Hydrogen polysulfides (H2Sn, n > 1) influence a variety of important biological functions and activities associated with hydrogen sulfide (H2S). The development of probes for rapid, selective, and sensitive detection of H2Sn still remains a great challenge. Lysosome plays crucial roles in various physiological processes among living cells, which arouse high interest in detecting endogenous lysosome-targetable H2Sn. To the best of our knowledge, there is no lysosome-targetable probe for H2Sn has been reported. In this work, a new lysosome-targetable probe NIPYNF, based on the scaffold of imidazo [1,5-]pyridine, with a large Stokes shift (215 nm), low detection limit (84 nM), fast response time (6 min) and low cytotoxicity was designed and synthesized. Furthermore, NIPYNF was successfully applied into the cell imaging for detection of endogenous lysosome-targetable H2Sn.
[Display omitted]
•A novel lysosome-targetable probe NIPYNF was reported.•NIPYNF could discriminate H2Sn over other analytes with high sensitivity, a low detection limit and a large Stokes shift.•NIPYNF could detect the endogenous H2Sn in lysosome of living cells.</description><subject>A549 Cells</subject><subject>Cell imaging</subject><subject>Cell Survival</subject><subject>Cells (biology)</subject><subject>Cytotoxicity</subject><subject>Fluorescent Dyes - metabolism</subject><subject>Fluorescent indicators</subject><subject>Fluorescent probe</subject><subject>Humans</subject><subject>Hydrogen</subject><subject>Hydrogen polysulfides</subject><subject>Hydrogen sulfide</subject><subject>Hydrogen Sulfide - chemistry</subject><subject>Hydrogen Sulfide - metabolism</subject><subject>Hydrogen-Ion Concentration</subject><subject>Imidazo[1,5-]pyridine</subject><subject>Lysosome</subject><subject>Lysosomes - metabolism</subject><subject>Optical Imaging</subject><subject>Polysulfides</subject><subject>Pyridines</subject><subject>Response time</subject><subject>Spectrometry, Fluorescence</subject><subject>Toxicity</subject><issn>0003-2670</issn><issn>1873-4324</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kUGP1CAYhonRuLOrP8CLIfHipZWP0kLjabNR12QTD-qZtPRjhrEDI9DdzC_wb0szqwcPnoDwvB8v70vIK2A1MOje7evBDDVnoGrgNWvhCdmAkk0lGi6ekg1jrKl4J9kFuUxpX44cmHhOLhomeylaviG_rqnHBzqfUkjhgFUe4hbzMM5I7byEiMmgz_QYw4j0weUdHei8MvRrDj8w0bRzNlMbIp0wo8kueBosRT-FLfqwJLo7TXHd02MozyyzdVPROU9nd-_8lhqc5_SCPLPDnPDl43pFvn_88O3mtrr78unzzfVdZRoFubIGhLId9ApsJxSCGIGz3nay76XlrZACOrBKIRvbiSnBpLFCdqJcyFbY5oq8Pc8tP_q5YMr64NLqYPBYzGoOqlUSJLCCvvkH3Ycl-uJO85KjUqyUUCg4UyaGlCJafYzuMMSTBqbXlvRel5b02pIGrktLRfP6cfIyHnD6q_hTSwHenwEsUdw7jDoZh97g5GLJWE_B_Wf8b6PnonY</recordid><startdate>20190516</startdate><enddate>20190516</enddate><creator>Ren, Yanshen</creator><creator>Zhang, Lulu</creator><creator>Zhou, Ziyan</creator><creator>Luo, Yanjun</creator><creator>Wang, Suilou</creator><creator>Yuan, Shengtao</creator><creator>Gu, Yueqing</creator><creator>Xu, Yungen</creator><creator>Zha, Xiaoming</creator><general>Elsevier B.V</general><general>Elsevier BV</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QP</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7T7</scope><scope>7TA</scope><scope>7TB</scope><scope>7TK</scope><scope>7TM</scope><scope>7U5</scope><scope>7U7</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-1430-4067</orcidid><orcidid>https://orcid.org/0000-0002-9506-2102</orcidid></search><sort><creationdate>20190516</creationdate><title>A new lysosome-targetable fluorescent probe with a large Stokes shift for detection of endogenous hydrogen polysulfides in living cells</title><author>Ren, Yanshen ; Zhang, Lulu ; Zhou, Ziyan ; Luo, Yanjun ; Wang, Suilou ; Yuan, Shengtao ; Gu, Yueqing ; Xu, Yungen ; Zha, Xiaoming</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c381t-fc148f61981f648e14b1209f67997f25474161f88e0b5d08407cf4764474754f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>A549 Cells</topic><topic>Cell imaging</topic><topic>Cell Survival</topic><topic>Cells (biology)</topic><topic>Cytotoxicity</topic><topic>Fluorescent Dyes - metabolism</topic><topic>Fluorescent indicators</topic><topic>Fluorescent probe</topic><topic>Humans</topic><topic>Hydrogen</topic><topic>Hydrogen polysulfides</topic><topic>Hydrogen sulfide</topic><topic>Hydrogen Sulfide - chemistry</topic><topic>Hydrogen Sulfide - metabolism</topic><topic>Hydrogen-Ion Concentration</topic><topic>Imidazo[1,5-]pyridine</topic><topic>Lysosome</topic><topic>Lysosomes - metabolism</topic><topic>Optical Imaging</topic><topic>Polysulfides</topic><topic>Pyridines</topic><topic>Response time</topic><topic>Spectrometry, Fluorescence</topic><topic>Toxicity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ren, Yanshen</creatorcontrib><creatorcontrib>Zhang, Lulu</creatorcontrib><creatorcontrib>Zhou, Ziyan</creatorcontrib><creatorcontrib>Luo, Yanjun</creatorcontrib><creatorcontrib>Wang, Suilou</creatorcontrib><creatorcontrib>Yuan, Shengtao</creatorcontrib><creatorcontrib>Gu, Yueqing</creatorcontrib><creatorcontrib>Xu, Yungen</creatorcontrib><creatorcontrib>Zha, Xiaoming</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Toxicology Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Analytica chimica acta</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ren, Yanshen</au><au>Zhang, Lulu</au><au>Zhou, Ziyan</au><au>Luo, Yanjun</au><au>Wang, Suilou</au><au>Yuan, Shengtao</au><au>Gu, Yueqing</au><au>Xu, Yungen</au><au>Zha, Xiaoming</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A new lysosome-targetable fluorescent probe with a large Stokes shift for detection of endogenous hydrogen polysulfides in living cells</atitle><jtitle>Analytica chimica acta</jtitle><addtitle>Anal Chim Acta</addtitle><date>2019-05-16</date><risdate>2019</risdate><volume>1056</volume><spage>117</spage><epage>124</epage><pages>117-124</pages><issn>0003-2670</issn><eissn>1873-4324</eissn><abstract>Hydrogen polysulfides (H2Sn, n > 1) influence a variety of important biological functions and activities associated with hydrogen sulfide (H2S). The development of probes for rapid, selective, and sensitive detection of H2Sn still remains a great challenge. Lysosome plays crucial roles in various physiological processes among living cells, which arouse high interest in detecting endogenous lysosome-targetable H2Sn. To the best of our knowledge, there is no lysosome-targetable probe for H2Sn has been reported. In this work, a new lysosome-targetable probe NIPYNF, based on the scaffold of imidazo [1,5-]pyridine, with a large Stokes shift (215 nm), low detection limit (84 nM), fast response time (6 min) and low cytotoxicity was designed and synthesized. Furthermore, NIPYNF was successfully applied into the cell imaging for detection of endogenous lysosome-targetable H2Sn.
[Display omitted]
•A novel lysosome-targetable probe NIPYNF was reported.•NIPYNF could discriminate H2Sn over other analytes with high sensitivity, a low detection limit and a large Stokes shift.•NIPYNF could detect the endogenous H2Sn in lysosome of living cells.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>30797452</pmid><doi>10.1016/j.aca.2018.12.051</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0003-1430-4067</orcidid><orcidid>https://orcid.org/0000-0002-9506-2102</orcidid></addata></record> |
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| subjects | A549 Cells Cell imaging Cell Survival Cells (biology) Cytotoxicity Fluorescent Dyes - metabolism Fluorescent indicators Fluorescent probe Humans Hydrogen Hydrogen polysulfides Hydrogen sulfide Hydrogen Sulfide - chemistry Hydrogen Sulfide - metabolism Hydrogen-Ion Concentration Imidazo[1,5-]pyridine Lysosome Lysosomes - metabolism Optical Imaging Polysulfides Pyridines Response time Spectrometry, Fluorescence Toxicity |
| title | A new lysosome-targetable fluorescent probe with a large Stokes shift for detection of endogenous hydrogen polysulfides in living cells |
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