Induction of Th2-related immune responses and production of systemic IgA in mice intranasally immunized with Brucella abortus malate dehydrogenase loaded chitosan nanoparticles
[Display omitted] The aim of this study was to investigate the induction of mucosal immune responses by an important Brucella abortus antigen, malate dehydrogenase (Mdh), loaded in mucoadhesive chitosan nanoparticles (CNs) and immunized intranasally in a BALB/c mouse model. The production of cytokin...
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| Veröffentlicht in: | Vaccine 2019-03, Vol.37 (12), p.1554-1564 |
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| creator | Soh, Sang Hee Shim, Soojin Im, Young Bin Park, Hong-Tae Cho, Chong-Su Yoo, Han Sang |
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The aim of this study was to investigate the induction of mucosal immune responses by an important Brucella abortus antigen, malate dehydrogenase (Mdh), loaded in mucoadhesive chitosan nanoparticles (CNs) and immunized intranasally in a BALB/c mouse model. The production of cytokines was investigated in human leukemic monocyte cells (THP-1 cells) after stimulation with the nanoparticles. Mdh-loaded CNs (CNs-Mdh) induced higher interleukin (IL)-6 production than unloaded antigens and TF loaded CNs (CNs-TF). Using ELISpot to quantify cytokines and antibody-secreting cells in the intranasally immunized mice, IL-4 and IgG-secreting cells were found to be significantly increased at 4 weeks and 6 weeks post-immunization in the CNs-Mdh immunized group, respectively. Increases in Mdh-specific IgG, IgG1, and IgG2a antibodies were confirmed at 6 weeks after immunization, indicating a predominant IgG1 response. Analysis of the mucosal immune response in the intranasally immunized mice revealed, Mdh-specific IgA and total IgA in the nasal washes, genital secretions, fecal extracts and sera that were remarkably increased in the CNs-Mdh-immunized group compared to the CNs-TF-immunized group except total IgA of nasal wash. Therefore, the results indicated that the intranasal immunization of CNs-loaded B. abortus Mdh antigen effectively induced antigen-specific mucosal immune responses through the elicitation of Th2-related immune responses. |
| doi_str_mv | 10.1016/j.vaccine.2019.02.005 |
| format | Article |
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The aim of this study was to investigate the induction of mucosal immune responses by an important Brucella abortus antigen, malate dehydrogenase (Mdh), loaded in mucoadhesive chitosan nanoparticles (CNs) and immunized intranasally in a BALB/c mouse model. The production of cytokines was investigated in human leukemic monocyte cells (THP-1 cells) after stimulation with the nanoparticles. Mdh-loaded CNs (CNs-Mdh) induced higher interleukin (IL)-6 production than unloaded antigens and TF loaded CNs (CNs-TF). Using ELISpot to quantify cytokines and antibody-secreting cells in the intranasally immunized mice, IL-4 and IgG-secreting cells were found to be significantly increased at 4 weeks and 6 weeks post-immunization in the CNs-Mdh immunized group, respectively. Increases in Mdh-specific IgG, IgG1, and IgG2a antibodies were confirmed at 6 weeks after immunization, indicating a predominant IgG1 response. Analysis of the mucosal immune response in the intranasally immunized mice revealed, Mdh-specific IgA and total IgA in the nasal washes, genital secretions, fecal extracts and sera that were remarkably increased in the CNs-Mdh-immunized group compared to the CNs-TF-immunized group except total IgA of nasal wash. Therefore, the results indicated that the intranasal immunization of CNs-loaded B. abortus Mdh antigen effectively induced antigen-specific mucosal immune responses through the elicitation of Th2-related immune responses.</description><identifier>ISSN: 0264-410X</identifier><identifier>EISSN: 1873-2518</identifier><identifier>DOI: 10.1016/j.vaccine.2019.02.005</identifier><identifier>PMID: 30792035</identifier><language>eng</language><publisher>Netherlands: Elsevier Ltd</publisher><subject>Animals ; Antigens ; Brucella abortus ; Brucellosis ; Chitosan ; Chitosan nanoparticles ; Dehydrogenases ; Drug delivery systems ; Immune response ; Immunization ; Immunoglobulin A ; Infections ; Lymphocytes T ; Malate dehydrogenase ; Mucosal immune response ; Mucous membrane ; Nanoparticles ; Nasal immunization ; Proteins ; Vaccines</subject><ispartof>Vaccine, 2019-03, Vol.37 (12), p.1554-1564</ispartof><rights>2019</rights><rights>Copyright © 2019. Published by Elsevier Ltd.</rights><rights>Copyright Elsevier Limited Mar 14, 2019</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c393t-3ca446e1d8a8c5e6d9578d10be3a94beb39d2d198bce01cd099de54be9c5e3cc3</citedby><cites>FETCH-LOGICAL-c393t-3ca446e1d8a8c5e6d9578d10be3a94beb39d2d198bce01cd099de54be9c5e3cc3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0264410X19301884$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30792035$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Soh, Sang Hee</creatorcontrib><creatorcontrib>Shim, Soojin</creatorcontrib><creatorcontrib>Im, Young Bin</creatorcontrib><creatorcontrib>Park, Hong-Tae</creatorcontrib><creatorcontrib>Cho, Chong-Su</creatorcontrib><creatorcontrib>Yoo, Han Sang</creatorcontrib><title>Induction of Th2-related immune responses and production of systemic IgA in mice intranasally immunized with Brucella abortus malate dehydrogenase loaded chitosan nanoparticles</title><title>Vaccine</title><addtitle>Vaccine</addtitle><description>[Display omitted]
The aim of this study was to investigate the induction of mucosal immune responses by an important Brucella abortus antigen, malate dehydrogenase (Mdh), loaded in mucoadhesive chitosan nanoparticles (CNs) and immunized intranasally in a BALB/c mouse model. The production of cytokines was investigated in human leukemic monocyte cells (THP-1 cells) after stimulation with the nanoparticles. Mdh-loaded CNs (CNs-Mdh) induced higher interleukin (IL)-6 production than unloaded antigens and TF loaded CNs (CNs-TF). Using ELISpot to quantify cytokines and antibody-secreting cells in the intranasally immunized mice, IL-4 and IgG-secreting cells were found to be significantly increased at 4 weeks and 6 weeks post-immunization in the CNs-Mdh immunized group, respectively. Increases in Mdh-specific IgG, IgG1, and IgG2a antibodies were confirmed at 6 weeks after immunization, indicating a predominant IgG1 response. Analysis of the mucosal immune response in the intranasally immunized mice revealed, Mdh-specific IgA and total IgA in the nasal washes, genital secretions, fecal extracts and sera that were remarkably increased in the CNs-Mdh-immunized group compared to the CNs-TF-immunized group except total IgA of nasal wash. Therefore, the results indicated that the intranasal immunization of CNs-loaded B. abortus Mdh antigen effectively induced antigen-specific mucosal immune responses through the elicitation of Th2-related immune responses.</description><subject>Animals</subject><subject>Antigens</subject><subject>Brucella abortus</subject><subject>Brucellosis</subject><subject>Chitosan</subject><subject>Chitosan nanoparticles</subject><subject>Dehydrogenases</subject><subject>Drug delivery systems</subject><subject>Immune response</subject><subject>Immunization</subject><subject>Immunoglobulin A</subject><subject>Infections</subject><subject>Lymphocytes T</subject><subject>Malate dehydrogenase</subject><subject>Mucosal immune response</subject><subject>Mucous membrane</subject><subject>Nanoparticles</subject><subject>Nasal 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in mice intranasally immunized with Brucella abortus malate dehydrogenase loaded chitosan nanoparticles</atitle><jtitle>Vaccine</jtitle><addtitle>Vaccine</addtitle><date>2019-03-14</date><risdate>2019</risdate><volume>37</volume><issue>12</issue><spage>1554</spage><epage>1564</epage><pages>1554-1564</pages><issn>0264-410X</issn><eissn>1873-2518</eissn><abstract>[Display omitted]
The aim of this study was to investigate the induction of mucosal immune responses by an important Brucella abortus antigen, malate dehydrogenase (Mdh), loaded in mucoadhesive chitosan nanoparticles (CNs) and immunized intranasally in a BALB/c mouse model. The production of cytokines was investigated in human leukemic monocyte cells (THP-1 cells) after stimulation with the nanoparticles. Mdh-loaded CNs (CNs-Mdh) induced higher interleukin (IL)-6 production than unloaded antigens and TF loaded CNs (CNs-TF). Using ELISpot to quantify cytokines and antibody-secreting cells in the intranasally immunized mice, IL-4 and IgG-secreting cells were found to be significantly increased at 4 weeks and 6 weeks post-immunization in the CNs-Mdh immunized group, respectively. Increases in Mdh-specific IgG, IgG1, and IgG2a antibodies were confirmed at 6 weeks after immunization, indicating a predominant IgG1 response. Analysis of the mucosal immune response in the intranasally immunized mice revealed, Mdh-specific IgA and total IgA in the nasal washes, genital secretions, fecal extracts and sera that were remarkably increased in the CNs-Mdh-immunized group compared to the CNs-TF-immunized group except total IgA of nasal wash. Therefore, the results indicated that the intranasal immunization of CNs-loaded B. abortus Mdh antigen effectively induced antigen-specific mucosal immune responses through the elicitation of Th2-related immune responses.</abstract><cop>Netherlands</cop><pub>Elsevier Ltd</pub><pmid>30792035</pmid><doi>10.1016/j.vaccine.2019.02.005</doi><tpages>11</tpages></addata></record> |
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| source | Elsevier ScienceDirect Journals |
| subjects | Animals Antigens Brucella abortus Brucellosis Chitosan Chitosan nanoparticles Dehydrogenases Drug delivery systems Immune response Immunization Immunoglobulin A Infections Lymphocytes T Malate dehydrogenase Mucosal immune response Mucous membrane Nanoparticles Nasal immunization Proteins Vaccines |
| title | Induction of Th2-related immune responses and production of systemic IgA in mice intranasally immunized with Brucella abortus malate dehydrogenase loaded chitosan nanoparticles |
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