Determination of alkaline phosphatase activity based on enzyme-triggered generation of a thiol and the fluorescence quenching of silver nanoclusters
A fluorimetric method is described for the determination of alkaline phosphatase (ALP) activity. It is based on the use of polyethyleneimine-coated silver nanoclusters (AgNCs), which display an intense blue fluorescence peaking at 450 nm (under 375 nm excitation). ALP catalyzes the dephosphorylation...
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| Veröffentlicht in: | Mikrochimica acta (1966) 2019-03, Vol.186 (3), p.180-180, Article 180 |
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| container_title | Mikrochimica acta (1966) |
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| creator | Luo, Minchuan Su, Zhu Wang, Xinyi Li, Liang Tu, Yifeng Yan, Jilin |
| description | A fluorimetric method is described for the determination of alkaline phosphatase (ALP) activity. It is based on the use of polyethyleneimine-coated silver nanoclusters (AgNCs), which display an intense blue fluorescence peaking at 450 nm (under 375 nm excitation). ALP catalyzes the dephosphorylation of the thiophosphate amifostine to generate a thiol that binds to the AgNCs and causes its fluorescence to be quenched. Under the optimal experimental conditions, fluorescence linearly drops in the 0.08–2.0 U L
−1
ALP activity range, and the limit of detection is 0.02 U L
−1
. The method was successfully applied to the determination of ALP activity in spiked human serum samples.
Graphical abstract
Alkaline phosphatase (ALP) catalyzes the degradation of amifostine with a generation a thiol product. The thiol quenches the fluorescence of silver nanoclusters, and a method for the detection of ALP down to 0.02 U L
−1
was developed. |
| doi_str_mv | 10.1007/s00604-019-3301-4 |
| format | Article |
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−1
ALP activity range, and the limit of detection is 0.02 U L
−1
. The method was successfully applied to the determination of ALP activity in spiked human serum samples.
Graphical abstract
Alkaline phosphatase (ALP) catalyzes the degradation of amifostine with a generation a thiol product. The thiol quenches the fluorescence of silver nanoclusters, and a method for the detection of ALP down to 0.02 U L
−1
was developed.</description><identifier>ISSN: 0026-3672</identifier><identifier>EISSN: 1436-5073</identifier><identifier>DOI: 10.1007/s00604-019-3301-4</identifier><identifier>PMID: 30771096</identifier><language>eng</language><publisher>Vienna: Springer Vienna</publisher><subject>Alkaline Phosphatase - blood ; Alkaline Phosphatase - chemistry ; Amifostine - chemistry ; Analytical Chemistry ; Characterization and Evaluation of Materials ; Chemistry ; Chemistry and Materials Science ; Enzyme Assays - methods ; Enzymes ; Fluorescence ; Fluorometry - methods ; Humans ; Limit of Detection ; Metal Nanoparticles - chemistry ; Metal Nanoparticles - radiation effects ; Microengineering ; Nanochemistry ; Nanotechnology ; Original Paper ; Phosphatases ; Polyethyleneimine - chemistry ; Silver - chemistry ; Sulfhydryl Compounds - chemical synthesis ; Thiols ; Ultraviolet Rays</subject><ispartof>Mikrochimica acta (1966), 2019-03, Vol.186 (3), p.180-180, Article 180</ispartof><rights>Springer-Verlag GmbH Austria, part of Springer Nature 2019</rights><rights>COPYRIGHT 2019 Springer</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c383t-98bdb226683714216df62bac069b8fbad68befc366a6058ada81bb8e8f5bab113</citedby><cites>FETCH-LOGICAL-c383t-98bdb226683714216df62bac069b8fbad68befc366a6058ada81bb8e8f5bab113</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00604-019-3301-4$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00604-019-3301-4$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30771096$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Luo, Minchuan</creatorcontrib><creatorcontrib>Su, Zhu</creatorcontrib><creatorcontrib>Wang, Xinyi</creatorcontrib><creatorcontrib>Li, Liang</creatorcontrib><creatorcontrib>Tu, Yifeng</creatorcontrib><creatorcontrib>Yan, Jilin</creatorcontrib><title>Determination of alkaline phosphatase activity based on enzyme-triggered generation of a thiol and the fluorescence quenching of silver nanoclusters</title><title>Mikrochimica acta (1966)</title><addtitle>Microchim Acta</addtitle><addtitle>Mikrochim Acta</addtitle><description>A fluorimetric method is described for the determination of alkaline phosphatase (ALP) activity. It is based on the use of polyethyleneimine-coated silver nanoclusters (AgNCs), which display an intense blue fluorescence peaking at 450 nm (under 375 nm excitation). ALP catalyzes the dephosphorylation of the thiophosphate amifostine to generate a thiol that binds to the AgNCs and causes its fluorescence to be quenched. Under the optimal experimental conditions, fluorescence linearly drops in the 0.08–2.0 U L
−1
ALP activity range, and the limit of detection is 0.02 U L
−1
. The method was successfully applied to the determination of ALP activity in spiked human serum samples.
Graphical abstract
Alkaline phosphatase (ALP) catalyzes the degradation of amifostine with a generation a thiol product. The thiol quenches the fluorescence of silver nanoclusters, and a method for the detection of ALP down to 0.02 U L
−1
was developed.</description><subject>Alkaline Phosphatase - blood</subject><subject>Alkaline Phosphatase - chemistry</subject><subject>Amifostine - chemistry</subject><subject>Analytical Chemistry</subject><subject>Characterization and Evaluation of Materials</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Enzyme Assays - methods</subject><subject>Enzymes</subject><subject>Fluorescence</subject><subject>Fluorometry - methods</subject><subject>Humans</subject><subject>Limit of Detection</subject><subject>Metal Nanoparticles - chemistry</subject><subject>Metal Nanoparticles - radiation effects</subject><subject>Microengineering</subject><subject>Nanochemistry</subject><subject>Nanotechnology</subject><subject>Original Paper</subject><subject>Phosphatases</subject><subject>Polyethyleneimine - chemistry</subject><subject>Silver - chemistry</subject><subject>Sulfhydryl Compounds - chemical synthesis</subject><subject>Thiols</subject><subject>Ultraviolet Rays</subject><issn>0026-3672</issn><issn>1436-5073</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9UcuOFCEUJUbjtKMf4MawdFMjjyqKWk7G8ZFM4kbXBKhLNyMFLVCTtN_hB0unRt0ZFpd7Oedwcg9Crym5ooSM7wohgvQdoVPHOaFd_wTtaM9FN5CRP0U7QpjouBjZBXpRyj0hdBSsf44uOBlHSiaxQ7_eQ4W8-KirTxEnh3X4roOPgI-HVI4HXXUBrG31D76esGndjBsS4s_TAl3Nfr-H3GZ7iJD_qeB68ClgHed2A-zCmjIUC9EC_rG2cvBxf0YWHx4g46hjsmEtzU15iZ45HQq8eqyX6NuH2683n7q7Lx8_31zfdZZLXrtJmtkwJoTkI-0ZFbMTzGhLxGSkM3oW0oCzXAgtyCD1rCU1RoJ0g9GGUn6J3m66x5yap1LV4pvFEHSEtBbFqByGfpKSNejVBt3rAMpHl2rWtp0ZFm9TBOfb_HqkQ_uJszOBbgSbUykZnDpmv-h8UpSoc3pqS0-19NQ5PdU3zptHP6tZYP7L-BNXA7ANUNpTbHtX92nNse3oP6q_Ac9dqOU</recordid><startdate>20190301</startdate><enddate>20190301</enddate><creator>Luo, Minchuan</creator><creator>Su, Zhu</creator><creator>Wang, Xinyi</creator><creator>Li, Liang</creator><creator>Tu, Yifeng</creator><creator>Yan, Jilin</creator><general>Springer Vienna</general><general>Springer</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20190301</creationdate><title>Determination of alkaline phosphatase activity based on enzyme-triggered generation of a thiol and the fluorescence quenching of silver nanoclusters</title><author>Luo, Minchuan ; Su, Zhu ; Wang, Xinyi ; Li, Liang ; Tu, Yifeng ; Yan, Jilin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c383t-98bdb226683714216df62bac069b8fbad68befc366a6058ada81bb8e8f5bab113</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Alkaline Phosphatase - blood</topic><topic>Alkaline Phosphatase - chemistry</topic><topic>Amifostine - chemistry</topic><topic>Analytical Chemistry</topic><topic>Characterization and Evaluation of Materials</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Enzyme Assays - methods</topic><topic>Enzymes</topic><topic>Fluorescence</topic><topic>Fluorometry - methods</topic><topic>Humans</topic><topic>Limit of Detection</topic><topic>Metal Nanoparticles - chemistry</topic><topic>Metal Nanoparticles - radiation effects</topic><topic>Microengineering</topic><topic>Nanochemistry</topic><topic>Nanotechnology</topic><topic>Original Paper</topic><topic>Phosphatases</topic><topic>Polyethyleneimine - chemistry</topic><topic>Silver - chemistry</topic><topic>Sulfhydryl Compounds - chemical synthesis</topic><topic>Thiols</topic><topic>Ultraviolet Rays</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Luo, Minchuan</creatorcontrib><creatorcontrib>Su, Zhu</creatorcontrib><creatorcontrib>Wang, Xinyi</creatorcontrib><creatorcontrib>Li, Liang</creatorcontrib><creatorcontrib>Tu, Yifeng</creatorcontrib><creatorcontrib>Yan, Jilin</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Mikrochimica acta (1966)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Luo, Minchuan</au><au>Su, Zhu</au><au>Wang, Xinyi</au><au>Li, Liang</au><au>Tu, Yifeng</au><au>Yan, Jilin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Determination of alkaline phosphatase activity based on enzyme-triggered generation of a thiol and the fluorescence quenching of silver nanoclusters</atitle><jtitle>Mikrochimica acta (1966)</jtitle><stitle>Microchim Acta</stitle><addtitle>Mikrochim Acta</addtitle><date>2019-03-01</date><risdate>2019</risdate><volume>186</volume><issue>3</issue><spage>180</spage><epage>180</epage><pages>180-180</pages><artnum>180</artnum><issn>0026-3672</issn><eissn>1436-5073</eissn><abstract>A fluorimetric method is described for the determination of alkaline phosphatase (ALP) activity. It is based on the use of polyethyleneimine-coated silver nanoclusters (AgNCs), which display an intense blue fluorescence peaking at 450 nm (under 375 nm excitation). ALP catalyzes the dephosphorylation of the thiophosphate amifostine to generate a thiol that binds to the AgNCs and causes its fluorescence to be quenched. Under the optimal experimental conditions, fluorescence linearly drops in the 0.08–2.0 U L
−1
ALP activity range, and the limit of detection is 0.02 U L
−1
. The method was successfully applied to the determination of ALP activity in spiked human serum samples.
Graphical abstract
Alkaline phosphatase (ALP) catalyzes the degradation of amifostine with a generation a thiol product. The thiol quenches the fluorescence of silver nanoclusters, and a method for the detection of ALP down to 0.02 U L
−1
was developed.</abstract><cop>Vienna</cop><pub>Springer Vienna</pub><pmid>30771096</pmid><doi>10.1007/s00604-019-3301-4</doi><tpages>1</tpages></addata></record> |
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| subjects | Alkaline Phosphatase - blood Alkaline Phosphatase - chemistry Amifostine - chemistry Analytical Chemistry Characterization and Evaluation of Materials Chemistry Chemistry and Materials Science Enzyme Assays - methods Enzymes Fluorescence Fluorometry - methods Humans Limit of Detection Metal Nanoparticles - chemistry Metal Nanoparticles - radiation effects Microengineering Nanochemistry Nanotechnology Original Paper Phosphatases Polyethyleneimine - chemistry Silver - chemistry Sulfhydryl Compounds - chemical synthesis Thiols Ultraviolet Rays |
| title | Determination of alkaline phosphatase activity based on enzyme-triggered generation of a thiol and the fluorescence quenching of silver nanoclusters |
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