The Utility of Phosphohistone H3 (PHH3) in Follicular Lymphoma Grading: A Comparative Study With Ki-67 and H&E Mitotic Count
To determine the utility of phosphohistone H3 (PHH3) mitotic count (MC) in grading follicular lymphoma (FL). FL cases were identified (2005-2017). Three hematopathologists recorded their average Ki-67 proliferation index, MC/high-power field (hpf) using PHH3 and H&E stains, and number of centrob...
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| Veröffentlicht in: | American journal of clinical pathology 2019-06, Vol.151 (6), p.542-550 |
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| creator | Khieu, Michelle L Broadwater, Devin R Aden, James K Coviello, Jean M Lynch, David T Hall, Jordan M |
| description | To determine the utility of phosphohistone H3 (PHH3) mitotic count (MC) in grading follicular lymphoma (FL).
FL cases were identified (2005-2017). Three hematopathologists recorded their average Ki-67 proliferation index, MC/high-power field (hpf) using PHH3 and H&E stains, and number of centroblasts/hpf. Results were assessed for correlations and interobserver variability.
Forty-three cases of FL were studied. PHH3 MC resulted in the strongest correlation to grade (r = 0.701, P < .0001) compared with Ki-67 proliferation index (PI) (r = 0.681, P < .0001) and H&E MC (r = 0.536, P = .0002) and the strongest linear relationship to centroblast count (R2 = 0.453). Agreement among pathologists was strongest for PHH3 (intraclass correlation coefficient [ICC] = 0.86) followed by Ki-67 PI (ICC = 0.85) and H&E MC (ICC = 0.78).
PHH3 correlates best to histologic grade and has less interobserver variability compared with Ki-67 PI and H&E MC. These results support using PHH3 as an adjunct in FL grading. |
| doi_str_mv | 10.1093/AJCP/AQZ003 |
| format | Article |
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FL cases were identified (2005-2017). Three hematopathologists recorded their average Ki-67 proliferation index, MC/high-power field (hpf) using PHH3 and H&E stains, and number of centroblasts/hpf. Results were assessed for correlations and interobserver variability.
Forty-three cases of FL were studied. PHH3 MC resulted in the strongest correlation to grade (r = 0.701, P < .0001) compared with Ki-67 proliferation index (PI) (r = 0.681, P < .0001) and H&E MC (r = 0.536, P = .0002) and the strongest linear relationship to centroblast count (R2 = 0.453). Agreement among pathologists was strongest for PHH3 (intraclass correlation coefficient [ICC] = 0.86) followed by Ki-67 PI (ICC = 0.85) and H&E MC (ICC = 0.78).
PHH3 correlates best to histologic grade and has less interobserver variability compared with Ki-67 PI and H&E MC. These results support using PHH3 as an adjunct in FL grading.]]></description><identifier>ISSN: 0002-9173</identifier><identifier>EISSN: 1943-7722</identifier><identifier>DOI: 10.1093/AJCP/AQZ003</identifier><identifier>PMID: 30788495</identifier><language>eng</language><publisher>England: Oxford University Press</publisher><subject>Adult ; Aged ; Aged, 80 and over ; Diagnosis ; Health aspects ; Histology, Pathological ; Histones ; Histones - analysis ; Humans ; Immunohistochemistry ; Ki-67 Antigen - analysis ; Lymphoma ; Lymphoma, Follicular - pathology ; Lymphomas ; Methods ; Middle Aged ; Mitotic Index ; Neoplasm Grading ; Observer Variation ; Young Adult</subject><ispartof>American journal of clinical pathology, 2019-06, Vol.151 (6), p.542-550</ispartof><rights>American Society for Clinical Pathology, 2019.</rights><rights>COPYRIGHT 2019 Oxford University Press</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c384t-78b747ebde4a4f2b769a232ed722ea081e2af36fd6bdfc97b39399189b68458a3</citedby><cites>FETCH-LOGICAL-c384t-78b747ebde4a4f2b769a232ed722ea081e2af36fd6bdfc97b39399189b68458a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30788495$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Khieu, Michelle L</creatorcontrib><creatorcontrib>Broadwater, Devin R</creatorcontrib><creatorcontrib>Aden, James K</creatorcontrib><creatorcontrib>Coviello, Jean M</creatorcontrib><creatorcontrib>Lynch, David T</creatorcontrib><creatorcontrib>Hall, Jordan M</creatorcontrib><title>The Utility of Phosphohistone H3 (PHH3) in Follicular Lymphoma Grading: A Comparative Study With Ki-67 and H&E Mitotic Count</title><title>American journal of clinical pathology</title><addtitle>Am J Clin Pathol</addtitle><description><![CDATA[To determine the utility of phosphohistone H3 (PHH3) mitotic count (MC) in grading follicular lymphoma (FL).
FL cases were identified (2005-2017). Three hematopathologists recorded their average Ki-67 proliferation index, MC/high-power field (hpf) using PHH3 and H&E stains, and number of centroblasts/hpf. Results were assessed for correlations and interobserver variability.
Forty-three cases of FL were studied. PHH3 MC resulted in the strongest correlation to grade (r = 0.701, P < .0001) compared with Ki-67 proliferation index (PI) (r = 0.681, P < .0001) and H&E MC (r = 0.536, P = .0002) and the strongest linear relationship to centroblast count (R2 = 0.453). Agreement among pathologists was strongest for PHH3 (intraclass correlation coefficient [ICC] = 0.86) followed by Ki-67 PI (ICC = 0.85) and H&E MC (ICC = 0.78).
PHH3 correlates best to histologic grade and has less interobserver variability compared with Ki-67 PI and H&E MC. These results support using PHH3 as an adjunct in FL grading.]]></description><subject>Adult</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Diagnosis</subject><subject>Health aspects</subject><subject>Histology, Pathological</subject><subject>Histones</subject><subject>Histones - analysis</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Ki-67 Antigen - analysis</subject><subject>Lymphoma</subject><subject>Lymphoma, Follicular - pathology</subject><subject>Lymphomas</subject><subject>Methods</subject><subject>Middle Aged</subject><subject>Mitotic Index</subject><subject>Neoplasm Grading</subject><subject>Observer Variation</subject><subject>Young Adult</subject><issn>0002-9173</issn><issn>1943-7722</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNpt0d9r1TAUB_AgirtuPvkuAUEm0i2_1iR7K5dtVe_YFTcEX0LapmsubdMlqXDH_ngzNhWH5CEQPt_DyTkAvMHoACNJD4vPy_Vh8fUHQvQZWGDJaMY5Ic_BAiFEMok53QGvQtgghIlA7CXYoYgLweTRAtxddgZeRdvbuIWuhevOhalznQ3RjQaWFO6vy5J-gHaEp67vbT332sPVdkhq0PDM68aO18ewgEs3TNrraH8a-C3OzRZ-t7GDX2yWc6jHBpbvT-C5jS7aOuF5jHvgRav7YF4_3rvg6vTkcllmq4uzT8tildVUsJhxUXHGTdUYpllLKp5LTSgxTfql0UhgQ3RL87bJq6atJa-opFJiIatcsCOh6S7Yf6g7eXczmxDVYENt-l6Pxs1BEZxcinCZ6LsndONmP6buFKE5lRgzjv6qa90bZcfWRa_r-6KqyJlIY8c5S-rgPyqdxgy2TuNtbXr_J_DxIVB7F4I3rZq8HbTfKozU_a6V3tST0je3addJv31sda4G0_yxv5dLfwFE_6EU</recordid><startdate>20190601</startdate><enddate>20190601</enddate><creator>Khieu, Michelle L</creator><creator>Broadwater, Devin R</creator><creator>Aden, James K</creator><creator>Coviello, Jean M</creator><creator>Lynch, David T</creator><creator>Hall, Jordan M</creator><general>Oxford University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>20190601</creationdate><title>The Utility of Phosphohistone H3 (PHH3) in Follicular Lymphoma Grading: A Comparative Study With Ki-67 and H&E Mitotic Count</title><author>Khieu, Michelle L ; Broadwater, Devin R ; Aden, James K ; Coviello, Jean M ; Lynch, David T ; Hall, Jordan M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c384t-78b747ebde4a4f2b769a232ed722ea081e2af36fd6bdfc97b39399189b68458a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Diagnosis</topic><topic>Health aspects</topic><topic>Histology, Pathological</topic><topic>Histones</topic><topic>Histones - analysis</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Ki-67 Antigen - analysis</topic><topic>Lymphoma</topic><topic>Lymphoma, Follicular - pathology</topic><topic>Lymphomas</topic><topic>Methods</topic><topic>Middle Aged</topic><topic>Mitotic Index</topic><topic>Neoplasm Grading</topic><topic>Observer Variation</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Khieu, Michelle L</creatorcontrib><creatorcontrib>Broadwater, Devin R</creatorcontrib><creatorcontrib>Aden, James K</creatorcontrib><creatorcontrib>Coviello, Jean M</creatorcontrib><creatorcontrib>Lynch, David T</creatorcontrib><creatorcontrib>Hall, Jordan M</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Proquest Nursing & Allied Health Source</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>American journal of clinical pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Khieu, Michelle L</au><au>Broadwater, Devin R</au><au>Aden, James K</au><au>Coviello, Jean M</au><au>Lynch, David T</au><au>Hall, Jordan M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The Utility of Phosphohistone H3 (PHH3) in Follicular Lymphoma Grading: A Comparative Study With Ki-67 and H&E Mitotic Count</atitle><jtitle>American journal of clinical pathology</jtitle><addtitle>Am J Clin Pathol</addtitle><date>2019-06-01</date><risdate>2019</risdate><volume>151</volume><issue>6</issue><spage>542</spage><epage>550</epage><pages>542-550</pages><issn>0002-9173</issn><eissn>1943-7722</eissn><abstract><![CDATA[To determine the utility of phosphohistone H3 (PHH3) mitotic count (MC) in grading follicular lymphoma (FL).
FL cases were identified (2005-2017). Three hematopathologists recorded their average Ki-67 proliferation index, MC/high-power field (hpf) using PHH3 and H&E stains, and number of centroblasts/hpf. Results were assessed for correlations and interobserver variability.
Forty-three cases of FL were studied. PHH3 MC resulted in the strongest correlation to grade (r = 0.701, P < .0001) compared with Ki-67 proliferation index (PI) (r = 0.681, P < .0001) and H&E MC (r = 0.536, P = .0002) and the strongest linear relationship to centroblast count (R2 = 0.453). Agreement among pathologists was strongest for PHH3 (intraclass correlation coefficient [ICC] = 0.86) followed by Ki-67 PI (ICC = 0.85) and H&E MC (ICC = 0.78).
PHH3 correlates best to histologic grade and has less interobserver variability compared with Ki-67 PI and H&E MC. These results support using PHH3 as an adjunct in FL grading.]]></abstract><cop>England</cop><pub>Oxford University Press</pub><pmid>30788495</pmid><doi>10.1093/AJCP/AQZ003</doi><tpages>9</tpages></addata></record> |
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| identifier | ISSN: 0002-9173 |
| ispartof | American journal of clinical pathology, 2019-06, Vol.151 (6), p.542-550 |
| issn | 0002-9173 1943-7722 |
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| source | MEDLINE; Oxford University Press Journals All Titles (1996-Current); EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Adult Aged Aged, 80 and over Diagnosis Health aspects Histology, Pathological Histones Histones - analysis Humans Immunohistochemistry Ki-67 Antigen - analysis Lymphoma Lymphoma, Follicular - pathology Lymphomas Methods Middle Aged Mitotic Index Neoplasm Grading Observer Variation Young Adult |
| title | The Utility of Phosphohistone H3 (PHH3) in Follicular Lymphoma Grading: A Comparative Study With Ki-67 and H&E Mitotic Count |
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