First molecular detection of Mycoplasma wenyonii and the ectoparasite biodiversity in dairy water buffalo and cattle in Bohol, Philippines

Hemoplasmosis caused by Mycoplasma spp. have been associated with major economic losses in the global dairy production. Hemoplasma studies in the Philippines are limited despite its potential impact. This study mainly aimed to detect the presence of hemoplasma species in dairy water buffaloes and ca...

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Veröffentlicht in:Parasitology international 2019-06, Vol.70, p.77-81
Hauptverfasser: Ybañez, Adrian P., Ybañez, Rochelle Haidee D., Armonia, Reynald Klint M., Chico, James Knowell E., Ferraren, Kevin James V., Tapdasan, Emerson P., Salces, Caro B., Maurillo, Bon Christian A., Galon, Eloiza May S., Macalanda, Adrian Miki C., Moumouni, Paul Franck A., Xuan, Xuenan
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container_start_page 77
container_title Parasitology international
container_volume 70
creator Ybañez, Adrian P.
Ybañez, Rochelle Haidee D.
Armonia, Reynald Klint M.
Chico, James Knowell E.
Ferraren, Kevin James V.
Tapdasan, Emerson P.
Salces, Caro B.
Maurillo, Bon Christian A.
Galon, Eloiza May S.
Macalanda, Adrian Miki C.
Moumouni, Paul Franck A.
Xuan, Xuenan
description Hemoplasmosis caused by Mycoplasma spp. have been associated with major economic losses in the global dairy production. Hemoplasma studies in the Philippines are limited despite its potential impact. This study mainly aimed to detect the presence of hemoplasma species in dairy water buffaloes and cattle and know their ectoparasite biodiversity in Bohol, Philippines. Detection of Mycoplasma spp. was performed using peripheral blood smear examination (PBSE) and standard PCR using whole blood samples collected from 100 dairy water buffaloes and 40 dairy cattle. Available records on the average annual, monthly and daily milk production were compared between PCR-positive and PCR-negative animals. Ectoparasites were manually collected and identified. While PBSE results were all negative, PCR testing showed that 80% (80 water buffaloes and 32 cattle) were positive for Mycoplasma spp. On the other hand, a total of 1436 ectoparasites were collected (609 Haematopinus and 827 Rhipicephalus spp.). DNA sequencing revealed that obtained sequences (193 bp) from 7 animals were 99.5 to 100% similar to registered Mycoplasma wenyonii sequences. The study reports the first molecular characterization of M. wenyonii in the Philippines and probably the first detection in dairy water buffaloes in Southeast Asia.
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Hemoplasma studies in the Philippines are limited despite its potential impact. This study mainly aimed to detect the presence of hemoplasma species in dairy water buffaloes and cattle and know their ectoparasite biodiversity in Bohol, Philippines. Detection of Mycoplasma spp. was performed using peripheral blood smear examination (PBSE) and standard PCR using whole blood samples collected from 100 dairy water buffaloes and 40 dairy cattle. Available records on the average annual, monthly and daily milk production were compared between PCR-positive and PCR-negative animals. Ectoparasites were manually collected and identified. While PBSE results were all negative, PCR testing showed that 80% (80 water buffaloes and 32 cattle) were positive for Mycoplasma spp. On the other hand, a total of 1436 ectoparasites were collected (609 Haematopinus and 827 Rhipicephalus spp.). DNA sequencing revealed that obtained sequences (193 bp) from 7 animals were 99.5 to 100% similar to registered Mycoplasma wenyonii sequences. 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DNA sequencing revealed that obtained sequences (193 bp) from 7 animals were 99.5 to 100% similar to registered Mycoplasma wenyonii sequences. 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DNA sequencing revealed that obtained sequences (193 bp) from 7 animals were 99.5 to 100% similar to registered Mycoplasma wenyonii sequences. The study reports the first molecular characterization of M. wenyonii in the Philippines and probably the first detection in dairy water buffaloes in Southeast Asia.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>30776450</pmid><doi>10.1016/j.parint.2019.02.004</doi><tpages>5</tpages></addata></record>
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subjects Animals
Biodiversity
Buffaloes - parasitology
Cattle
Cattle Diseases - microbiology
Cattle Diseases - parasitology
Dairy cattle
Dairy water buffalo
Ectoparasites
Ectoparasitic Infestations - veterinary
Milk
Mycoplasma - genetics
Mycoplasma - isolation & purification
Mycoplasma Infections - veterinary
Mycoplasma wenyonii
PCR
Philippines
Polymerase Chain Reaction
Rhipicephalus - genetics
RNA, Ribosomal, 16S - genetics
Sequence Analysis, DNA
title First molecular detection of Mycoplasma wenyonii and the ectoparasite biodiversity in dairy water buffalo and cattle in Bohol, Philippines
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