Immobilization and stabilization of different β-glucosidases using the glutaraldehyde chemistry: Optimal protocol depends on the enzyme

Three β-glucosidases (Pectinex Ultra SP-L, Pectinex Ultra Clear and homemade preparation from Aspergillus niger) were immobilized using different strategies: ionic adsorption on aminated (MANAE)-agarose beads at pH 5, 7, and 9, followed by biocatalysts modification with glutaraldehyde, or on glutara...

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Veröffentlicht in:	International journal of biological macromolecules 2019-05, Vol.129, p.672-678
Hauptverfasser:	de Andrades, Diandra, Graebin, Natália G., Kadowaki, Marina Kimiko, Ayub, Marco A.Z., Fernandez-Lafuente, Roberto, Rodrigues, Rafael C.
Format:	Artikel
Sprache:	eng
Schlagworte:	Covalent attachment Enzyme stabilization Glutaraldehyde Ionic adsorption MANAE-agarose
Online-Zugang:	Volltext
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