A technical protocol for an experimental ex vivo model using arterially perfused porcine eyes
Ex vivo ocular perfused models have been described in the past and were applied in different mammalian species as platforms to test drug delivery systems and surgical techniques. However, reproduction of those methods is challenging because extensive and precise description of the protocols used is...
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| Veröffentlicht in: | Experimental eye research 2019-04, Vol.181, p.171-177 |
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| container_title | Experimental eye research |
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| creator | Rousou, C. Hoogenboom, P. van Overdam, K.A. Storm, G. Dorrestijn, J. Mastrobattista, E. |
| description | Ex vivo ocular perfused models have been described in the past and were applied in different mammalian species as platforms to test drug delivery systems and surgical techniques. However, reproduction of those methods is challenging because extensive and precise description of the protocols used is lacking. In this technical paper we provide a detailed description of all the steps to be followed from the enucleation of porcine eyes to cannulation of the ophthalmic artery and perfusion. This model can contribute to the reduction of use of living animals in ophthalmology research, whereas as opposed to in vitro models, it preserves tissue complexity and integrity. |
| doi_str_mv | 10.1016/j.exer.2019.02.003 |
| format | Article |
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This model can contribute to the reduction of use of living animals in ophthalmology research, whereas as opposed to in vitro models, it preserves tissue complexity and integrity.</description><subject>Animals</subject><subject>Extracorporeal model</subject><subject>Eye - blood supply</subject><subject>In Vitro Techniques - methods</subject><subject>Models, Animal</subject><subject>Ophthalmic Artery</subject><subject>Perfused porcine eye model</subject><subject>Perfusion - methods</subject><subject>Retinal perfusion</subject><subject>Retinal Vessels</subject><subject>Swine</subject><issn>0014-4835</issn><issn>1096-0007</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kM1KAzEYRYMotlZfwIVk6WbGL5P5BTel-AcFN7qUkMl80ZSZSU1mSvv2prS6FAKB5NwL9xByzSBmwPK7VYxbdHECrIohiQH4CZkyqPIIAIpTMgVgaZSWPJuQC-9X4ZWnRXpOJhwKnuVZMSUfczqg-uqNki1dOztYZVuqraOyp7hdozMd9kP4xC3dmI2lnW2wpaM3_SeVbgiAbNsdDaQePTZ0bZ0yPVLcob8kZ1q2Hq-O94y8Pz68LZ6j5evTy2K-jFQKMEQKslryVKWZwiZTCdcaSl7IXGYyYagrnmtd5zItsOK6hgxllZe6kHXZIG9qPiO3h96w4HtEP4jOeIVtK3u0oxcJK3k4Zc4CmhxQ5az3DrVYh4nS7QQDsdcqVmKvVey1CkhEkBZCN8f-se6w-Yv8egzA_QHAsHJjQtwrg32YYxyqQTTW_Nf_AyBCi0w</recordid><startdate>201904</startdate><enddate>201904</enddate><creator>Rousou, C.</creator><creator>Hoogenboom, P.</creator><creator>van Overdam, K.A.</creator><creator>Storm, G.</creator><creator>Dorrestijn, J.</creator><creator>Mastrobattista, E.</creator><general>Elsevier Ltd</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-5425-7210</orcidid></search><sort><creationdate>201904</creationdate><title>A technical protocol for an experimental ex vivo model using arterially perfused porcine eyes</title><author>Rousou, C. ; Hoogenboom, P. ; van Overdam, K.A. ; Storm, G. ; Dorrestijn, J. ; Mastrobattista, E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c400t-c05ba34c45ced5c23ff0837a6a5a21ef936ffb6a47e93fb05ea968f7ab8de3db3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Animals</topic><topic>Extracorporeal model</topic><topic>Eye - blood supply</topic><topic>In Vitro Techniques - methods</topic><topic>Models, Animal</topic><topic>Ophthalmic Artery</topic><topic>Perfused porcine eye model</topic><topic>Perfusion - methods</topic><topic>Retinal perfusion</topic><topic>Retinal Vessels</topic><topic>Swine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rousou, C.</creatorcontrib><creatorcontrib>Hoogenboom, P.</creatorcontrib><creatorcontrib>van Overdam, K.A.</creatorcontrib><creatorcontrib>Storm, G.</creatorcontrib><creatorcontrib>Dorrestijn, J.</creatorcontrib><creatorcontrib>Mastrobattista, E.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Experimental eye research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rousou, C.</au><au>Hoogenboom, P.</au><au>van Overdam, K.A.</au><au>Storm, G.</au><au>Dorrestijn, J.</au><au>Mastrobattista, E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A technical protocol for an experimental ex vivo model using arterially perfused porcine eyes</atitle><jtitle>Experimental eye research</jtitle><addtitle>Exp Eye Res</addtitle><date>2019-04</date><risdate>2019</risdate><volume>181</volume><spage>171</spage><epage>177</epage><pages>171-177</pages><issn>0014-4835</issn><eissn>1096-0007</eissn><abstract>Ex vivo ocular perfused models have been described in the past and were applied in different mammalian species as platforms to test drug delivery systems and surgical techniques. 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| ispartof | Experimental eye research, 2019-04, Vol.181, p.171-177 |
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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Animals Extracorporeal model Eye - blood supply In Vitro Techniques - methods Models, Animal Ophthalmic Artery Perfused porcine eye model Perfusion - methods Retinal perfusion Retinal Vessels Swine |
| title | A technical protocol for an experimental ex vivo model using arterially perfused porcine eyes |
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