Treponema pallidum induces the activation of endothelial cells via macrophage-derived exosomes
Recent studies have shown that exosomes play a role in pathogenesis and in the treatment of inflammatory diseases and tumours. We explored the effects of Treponema pallidum -induced macrophage-derived exosomes on vascular endothelial cells to determine whether they are involved in the pathogenesis o...
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| Veröffentlicht in: | Archives of Dermatological Research 2019-03, Vol.311 (2), p.121-130 |
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| creator | Xu, Bu-Fang Wang, Qian-Qiu Zhang, Jing-Ping Hu, Wen-Long Zhang, Rui-Li |
| description | Recent studies have shown that exosomes play a role in pathogenesis and in the treatment of inflammatory diseases and tumours. We explored the effects of
Treponema pallidum
-induced macrophage-derived exosomes on vascular endothelial cells to determine whether they are involved in the pathogenesis of syphilis. A syphilis infection model was established using rabbits to harvest
T. pallidum
at the peak of proliferation. Exosomes derived from macrophages were extracted using commercial kits and characterized by transmission electron microscopy, western blot assays, and nanoparticle tracking analysis. Secreted cytokine levels and the adhesion and permeability of human umbilical vein endothelial cells were evaluated in a co-culture model using the extracted exosomes. The results of this study revealed that exosomes derived from
T. pallidum
-infected macrophages enhanced cell adhesion and permeability. The levels of the secreted cytokines, including ICAM-1, VCAM-1, VEGF, and IL-8 were higher in the experimental group than in the control group. Our findings suggest that exosomes derived from
T. pallidum
-infected macrophages affect the cell adhesion and permeability of vascular endothelial cells. These changes may play important roles in syphilis pathogenesis. This study is the first to reveal the effects of exosomes derived from
T. pallidum
-infected macrophages on the adhesion, permeability, and secreted cytokines of human umbilical vein endothelial cells. |
| doi_str_mv | 10.1007/s00403-018-01888-4 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2179523099</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2179523099</sourcerecordid><originalsourceid>FETCH-LOGICAL-c375t-f548f0f683a55752b97c21d55bd26d51e6160685704aa70a6aff8136ca7663d73</originalsourceid><addsrcrecordid>eNp9kU9rFjEQxoNY7EvbL9CDBLx4WZ38zx6lWBUKXip4asi7mW1Tdjdrsvui396sb1XwYGAIZH7zZGYeQi4ZvGEA5m0BkCAaYHYLaxv5jOyYFLwB3X59TnYgJDRCt_qUXJTyCPUYkBzMC3IqwDAO1u7I3W3GOU04ejr7YYhhHWmcwtphocsDUt8t8eCXmCaaeopTSPV1iH6gHQ5DoYfo6ei7nOYHf49NwBwPGCh-TyWNWM7JSe-HghdP9xn5cv3-9upjc_P5w6erdzdNJ4xaml5J20OvrfBKGcX3rek4C0rtA9dBMdRMg7aqDuC9Aa9931smdOeN1iIYcUZeH3XnnL6tWBY3xrJ16CdMa3GcmVZxAW1b0Vf_oI9pzVPtbqNky4SVslL8SNXRSsnYuznH0ecfjoHbDHBHA1xdvvtlgNuKXj5Jr_sRw5-S3-uugDgCpaame8x___6P7E_tt5An</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2174913844</pqid></control><display><type>article</type><title>Treponema pallidum induces the activation of endothelial cells via macrophage-derived exosomes</title><source>MEDLINE</source><source>SpringerLink Journals - AutoHoldings</source><creator>Xu, Bu-Fang ; Wang, Qian-Qiu ; Zhang, Jing-Ping ; Hu, Wen-Long ; Zhang, Rui-Li</creator><creatorcontrib>Xu, Bu-Fang ; Wang, Qian-Qiu ; Zhang, Jing-Ping ; Hu, Wen-Long ; Zhang, Rui-Li</creatorcontrib><description>Recent studies have shown that exosomes play a role in pathogenesis and in the treatment of inflammatory diseases and tumours. We explored the effects of
Treponema pallidum
-induced macrophage-derived exosomes on vascular endothelial cells to determine whether they are involved in the pathogenesis of syphilis. A syphilis infection model was established using rabbits to harvest
T. pallidum
at the peak of proliferation. Exosomes derived from macrophages were extracted using commercial kits and characterized by transmission electron microscopy, western blot assays, and nanoparticle tracking analysis. Secreted cytokine levels and the adhesion and permeability of human umbilical vein endothelial cells were evaluated in a co-culture model using the extracted exosomes. The results of this study revealed that exosomes derived from
T. pallidum
-infected macrophages enhanced cell adhesion and permeability. The levels of the secreted cytokines, including ICAM-1, VCAM-1, VEGF, and IL-8 were higher in the experimental group than in the control group. Our findings suggest that exosomes derived from
T. pallidum
-infected macrophages affect the cell adhesion and permeability of vascular endothelial cells. These changes may play important roles in syphilis pathogenesis. This study is the first to reveal the effects of exosomes derived from
T. pallidum
-infected macrophages on the adhesion, permeability, and secreted cytokines of human umbilical vein endothelial cells.</description><identifier>ISSN: 0340-3696</identifier><identifier>EISSN: 1432-069X</identifier><identifier>DOI: 10.1007/s00403-018-01888-4</identifier><identifier>PMID: 30712088</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Animals ; Bacterial infections ; Capillary Permeability ; Cell activation ; Cell Adhesion ; Cell adhesion & migration ; Cell Communication ; Cell culture ; Cytokines ; Cytokines - metabolism ; Dermatology ; Disease Models, Animal ; Endothelial cells ; Endothelial Cells - microbiology ; Endothelial Cells - physiology ; Exosomes ; Exosomes - metabolism ; Human Umbilical Vein Endothelial Cells ; Humans ; Inflammatory diseases ; Intercellular adhesion molecule 1 ; Interleukin 8 ; Macrophages ; Macrophages - metabolism ; Medicine ; Medicine & Public Health ; Nanoparticles ; Original Paper ; Pathogenesis ; Permeability ; Rabbits ; Signal Transduction ; Syphilis ; Syphilis - immunology ; Transmission electron microscopy ; Treponema pallidum ; Treponema pallidum - physiology ; Tumors ; Umbilical vein ; Vascular cell adhesion molecule 1 ; Vascular endothelial growth factor</subject><ispartof>Archives of Dermatological Research, 2019-03, Vol.311 (2), p.121-130</ispartof><rights>Springer-Verlag GmbH Germany, part of Springer Nature 2019</rights><rights>Archives of Dermatological Research is a copyright of Springer, (2019). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c375t-f548f0f683a55752b97c21d55bd26d51e6160685704aa70a6aff8136ca7663d73</citedby><cites>FETCH-LOGICAL-c375t-f548f0f683a55752b97c21d55bd26d51e6160685704aa70a6aff8136ca7663d73</cites><orcidid>0000-0003-2707-1776</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00403-018-01888-4$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00403-018-01888-4$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30712088$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Xu, Bu-Fang</creatorcontrib><creatorcontrib>Wang, Qian-Qiu</creatorcontrib><creatorcontrib>Zhang, Jing-Ping</creatorcontrib><creatorcontrib>Hu, Wen-Long</creatorcontrib><creatorcontrib>Zhang, Rui-Li</creatorcontrib><title>Treponema pallidum induces the activation of endothelial cells via macrophage-derived exosomes</title><title>Archives of Dermatological Research</title><addtitle>Arch Dermatol Res</addtitle><addtitle>Arch Dermatol Res</addtitle><description>Recent studies have shown that exosomes play a role in pathogenesis and in the treatment of inflammatory diseases and tumours. We explored the effects of
Treponema pallidum
-induced macrophage-derived exosomes on vascular endothelial cells to determine whether they are involved in the pathogenesis of syphilis. A syphilis infection model was established using rabbits to harvest
T. pallidum
at the peak of proliferation. Exosomes derived from macrophages were extracted using commercial kits and characterized by transmission electron microscopy, western blot assays, and nanoparticle tracking analysis. Secreted cytokine levels and the adhesion and permeability of human umbilical vein endothelial cells were evaluated in a co-culture model using the extracted exosomes. The results of this study revealed that exosomes derived from
T. pallidum
-infected macrophages enhanced cell adhesion and permeability. The levels of the secreted cytokines, including ICAM-1, VCAM-1, VEGF, and IL-8 were higher in the experimental group than in the control group. Our findings suggest that exosomes derived from
T. pallidum
-infected macrophages affect the cell adhesion and permeability of vascular endothelial cells. These changes may play important roles in syphilis pathogenesis. This study is the first to reveal the effects of exosomes derived from
T. pallidum
-infected macrophages on the adhesion, permeability, and secreted cytokines of human umbilical vein endothelial cells.</description><subject>Animals</subject><subject>Bacterial infections</subject><subject>Capillary Permeability</subject><subject>Cell activation</subject><subject>Cell Adhesion</subject><subject>Cell adhesion & migration</subject><subject>Cell Communication</subject><subject>Cell culture</subject><subject>Cytokines</subject><subject>Cytokines - metabolism</subject><subject>Dermatology</subject><subject>Disease Models, Animal</subject><subject>Endothelial cells</subject><subject>Endothelial Cells - microbiology</subject><subject>Endothelial Cells - physiology</subject><subject>Exosomes</subject><subject>Exosomes - metabolism</subject><subject>Human Umbilical Vein Endothelial Cells</subject><subject>Humans</subject><subject>Inflammatory diseases</subject><subject>Intercellular adhesion molecule 1</subject><subject>Interleukin 8</subject><subject>Macrophages</subject><subject>Macrophages - metabolism</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Nanoparticles</subject><subject>Original Paper</subject><subject>Pathogenesis</subject><subject>Permeability</subject><subject>Rabbits</subject><subject>Signal Transduction</subject><subject>Syphilis</subject><subject>Syphilis - immunology</subject><subject>Transmission electron microscopy</subject><subject>Treponema pallidum</subject><subject>Treponema pallidum - physiology</subject><subject>Tumors</subject><subject>Umbilical vein</subject><subject>Vascular cell adhesion molecule 1</subject><subject>Vascular endothelial growth factor</subject><issn>0340-3696</issn><issn>1432-069X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kU9rFjEQxoNY7EvbL9CDBLx4WZ38zx6lWBUKXip4asi7mW1Tdjdrsvui396sb1XwYGAIZH7zZGYeQi4ZvGEA5m0BkCAaYHYLaxv5jOyYFLwB3X59TnYgJDRCt_qUXJTyCPUYkBzMC3IqwDAO1u7I3W3GOU04ejr7YYhhHWmcwtphocsDUt8t8eCXmCaaeopTSPV1iH6gHQ5DoYfo6ei7nOYHf49NwBwPGCh-TyWNWM7JSe-HghdP9xn5cv3-9upjc_P5w6erdzdNJ4xaml5J20OvrfBKGcX3rek4C0rtA9dBMdRMg7aqDuC9Aa9931smdOeN1iIYcUZeH3XnnL6tWBY3xrJ16CdMa3GcmVZxAW1b0Vf_oI9pzVPtbqNky4SVslL8SNXRSsnYuznH0ecfjoHbDHBHA1xdvvtlgNuKXj5Jr_sRw5-S3-uugDgCpaame8x___6P7E_tt5An</recordid><startdate>20190301</startdate><enddate>20190301</enddate><creator>Xu, Bu-Fang</creator><creator>Wang, Qian-Qiu</creator><creator>Zhang, Jing-Ping</creator><creator>Hu, Wen-Long</creator><creator>Zhang, Rui-Li</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>K9.</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-2707-1776</orcidid></search><sort><creationdate>20190301</creationdate><title>Treponema pallidum induces the activation of endothelial cells via macrophage-derived exosomes</title><author>Xu, Bu-Fang ; Wang, Qian-Qiu ; Zhang, Jing-Ping ; Hu, Wen-Long ; Zhang, Rui-Li</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c375t-f548f0f683a55752b97c21d55bd26d51e6160685704aa70a6aff8136ca7663d73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Animals</topic><topic>Bacterial infections</topic><topic>Capillary Permeability</topic><topic>Cell activation</topic><topic>Cell Adhesion</topic><topic>Cell adhesion & migration</topic><topic>Cell Communication</topic><topic>Cell culture</topic><topic>Cytokines</topic><topic>Cytokines - metabolism</topic><topic>Dermatology</topic><topic>Disease Models, Animal</topic><topic>Endothelial cells</topic><topic>Endothelial Cells - microbiology</topic><topic>Endothelial Cells - physiology</topic><topic>Exosomes</topic><topic>Exosomes - metabolism</topic><topic>Human Umbilical Vein Endothelial Cells</topic><topic>Humans</topic><topic>Inflammatory diseases</topic><topic>Intercellular adhesion molecule 1</topic><topic>Interleukin 8</topic><topic>Macrophages</topic><topic>Macrophages - metabolism</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Nanoparticles</topic><topic>Original Paper</topic><topic>Pathogenesis</topic><topic>Permeability</topic><topic>Rabbits</topic><topic>Signal Transduction</topic><topic>Syphilis</topic><topic>Syphilis - immunology</topic><topic>Transmission electron microscopy</topic><topic>Treponema pallidum</topic><topic>Treponema pallidum - physiology</topic><topic>Tumors</topic><topic>Umbilical vein</topic><topic>Vascular cell adhesion molecule 1</topic><topic>Vascular endothelial growth factor</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Xu, Bu-Fang</creatorcontrib><creatorcontrib>Wang, Qian-Qiu</creatorcontrib><creatorcontrib>Zhang, Jing-Ping</creatorcontrib><creatorcontrib>Hu, Wen-Long</creatorcontrib><creatorcontrib>Zhang, Rui-Li</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>Archives of Dermatological Research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Xu, Bu-Fang</au><au>Wang, Qian-Qiu</au><au>Zhang, Jing-Ping</au><au>Hu, Wen-Long</au><au>Zhang, Rui-Li</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Treponema pallidum induces the activation of endothelial cells via macrophage-derived exosomes</atitle><jtitle>Archives of Dermatological Research</jtitle><stitle>Arch Dermatol Res</stitle><addtitle>Arch Dermatol Res</addtitle><date>2019-03-01</date><risdate>2019</risdate><volume>311</volume><issue>2</issue><spage>121</spage><epage>130</epage><pages>121-130</pages><issn>0340-3696</issn><eissn>1432-069X</eissn><abstract>Recent studies have shown that exosomes play a role in pathogenesis and in the treatment of inflammatory diseases and tumours. We explored the effects of
Treponema pallidum
-induced macrophage-derived exosomes on vascular endothelial cells to determine whether they are involved in the pathogenesis of syphilis. A syphilis infection model was established using rabbits to harvest
T. pallidum
at the peak of proliferation. Exosomes derived from macrophages were extracted using commercial kits and characterized by transmission electron microscopy, western blot assays, and nanoparticle tracking analysis. Secreted cytokine levels and the adhesion and permeability of human umbilical vein endothelial cells were evaluated in a co-culture model using the extracted exosomes. The results of this study revealed that exosomes derived from
T. pallidum
-infected macrophages enhanced cell adhesion and permeability. The levels of the secreted cytokines, including ICAM-1, VCAM-1, VEGF, and IL-8 were higher in the experimental group than in the control group. Our findings suggest that exosomes derived from
T. pallidum
-infected macrophages affect the cell adhesion and permeability of vascular endothelial cells. These changes may play important roles in syphilis pathogenesis. This study is the first to reveal the effects of exosomes derived from
T. pallidum
-infected macrophages on the adhesion, permeability, and secreted cytokines of human umbilical vein endothelial cells.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>30712088</pmid><doi>10.1007/s00403-018-01888-4</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0003-2707-1776</orcidid></addata></record> |
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| ispartof | Archives of Dermatological Research, 2019-03, Vol.311 (2), p.121-130 |
| issn | 0340-3696 1432-069X |
| language | eng |
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| source | MEDLINE; SpringerLink Journals - AutoHoldings |
| subjects | Animals Bacterial infections Capillary Permeability Cell activation Cell Adhesion Cell adhesion & migration Cell Communication Cell culture Cytokines Cytokines - metabolism Dermatology Disease Models, Animal Endothelial cells Endothelial Cells - microbiology Endothelial Cells - physiology Exosomes Exosomes - metabolism Human Umbilical Vein Endothelial Cells Humans Inflammatory diseases Intercellular adhesion molecule 1 Interleukin 8 Macrophages Macrophages - metabolism Medicine Medicine & Public Health Nanoparticles Original Paper Pathogenesis Permeability Rabbits Signal Transduction Syphilis Syphilis - immunology Transmission electron microscopy Treponema pallidum Treponema pallidum - physiology Tumors Umbilical vein Vascular cell adhesion molecule 1 Vascular endothelial growth factor |
| title | Treponema pallidum induces the activation of endothelial cells via macrophage-derived exosomes |
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