Multicolor emitting N/S-doped carbon dots as a fluorescent probe for imaging pathogenic bacteria and human buccal epithelial cells
Carbon dots co-doped with nitrogen and sulfur (NSCDs) were obtained from thiourea and TAE (Tris-acetate-ethylenediamine) buffer using microwave assisted hydrothermal synthesis. The synergistic presence of nitrogen and sulfur as a dopant results in teasing fluorescence properties and a fluorescence q...
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Veröffentlicht in: | Mikrochimica acta (1966) 2019-03, Vol.186 (3), p.157-157, Article 157 |
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Sprache: | eng |
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Zusammenfassung: | Carbon dots co-doped with nitrogen and sulfur (NSCDs) were obtained from thiourea and TAE (Tris-acetate-ethylenediamine) buffer using microwave assisted hydrothermal synthesis. The synergistic presence of nitrogen and sulfur as a dopant results in teasing fluorescence properties and a fluorescence quantum yield of 57%. An HR-TEM study showed the NSCDs to be mono-dispersed and seemingly spherical with an average hydrodynamic diameter of 3.6 ± 0.88 nm. The NSCDs are nontoxic as proven by an MTT assay for cytotoxicity. The optical characterization was done by using UV-Vis absorption and fluorescence spectroscopy which revealed excitation wavelength-dependent multicolor emissions. The characterization of surface topology was done by using X-ray diffraction, FTIR, and X-ray photoelectron spectroscopy. The NSCDs were used to image various pathogenic bacteria
(E. coli, Klebsiella, Pseudomonas
&
Staphylococcus
) and human buccal epithelial cells by applying multicolor fluorometry.
Graphical abstract
Schematic presentation of microwave-assisted hydrothermal synthesis of nitrogen and sulfur doped carbon dots (NSCD) based on Thiourea and 50X Tris-acetate-ethylenediamine (TAE) buffer having multicolor fluorescence, used for tagging and imaging pathogenic bacteria and Human buccal epithelial cells using fluorescence microscope. |
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ISSN: | 0026-3672 1436-5073 |
DOI: | 10.1007/s00604-019-3270-7 |