Determination of Alzheimer biomarker DNA by using an electrode modified with in-situ precipitated molybdophosphate catalyzed by alkaline phosphatase-encapsulated DNA hydrogel and target recycling amplification
An electrochemical biosensor is described for highly sensitive determination of tDNA, an Alzheimer’s disease (AD)-related biomarker. Electroactive molybdophosphate anions were precipitated in-situ on a glassy carbon electrode (GCE) via catalytic hydrolysis by alkaline phosphatase (ALP). This is foll...
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| Veröffentlicht in: | Mikrochimica acta (1966) 2019-03, Vol.186 (3), p.158-158, Article 158 |
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| creator | Hua, Xiaoyu Zhou, Xingxing Guo, Shijing Zheng, Ting Yuan, Ruo Xu, Wenju |
| description | An electrochemical biosensor is described for highly sensitive determination of tDNA, an Alzheimer’s disease (AD)-related biomarker. Electroactive molybdophosphate anions were precipitated in-situ on a glassy carbon electrode (GCE) via catalytic hydrolysis by alkaline phosphatase (ALP). This is followed by recycling amplification of tDNA. Four DNA strands (referred to as S1, S2, S3 and S4) were designed to assemble X-shape DNA (X-DNA) building blocks. These were further extended into four directions under the action of DNA polymerase. The resultant two X-DNA motifs were polymerize. Simultaneously, ALP is encapsulated into a hydrogels network to obtain a porous material of type ALP@DNAhg. The GCE was modified with reduced graphene oxide functionalized with gold nanoparticles (Au@rGO). If ALP@DNAhg are captured via strand displacement, tDNA recycling assembly for signal amplification is initiated. This results in the immobilization of large amounts of ALP. On introduction of pyrophosphate and molybdate (MoO
4
2−
), ALP will catalyze the hydrolysis of pyrophosphate to produce phosphate. It will react with molybdate to form redox active phosphomolybdate anions (PMo
12
O
40
3−
). Its amperometrical signal depends on the concentration of tDNA in the 1.0 × 10
−2
to 1.0 × 10
4
pM concentration range, and the detection limit is 3.4 × 10
−3
pM.
Graphical abstract
Schematic presentation of (
a
) preparation of alkaline phosphatase-encapsulated DNA hydrogel (ALP@DNAhg). (
b
) fabrication of the biosensor for target DNA (tDNA) based on ALP@DNAhg to catalyze in situ precipitation of electroactive molybdophosphate anion (PMo
12
O
40
3−
) and tDNA recycling amplification, achieving tDNA-dependent electrochemical signal readout (X-DNA: X-shape DNA building block. TdT: terminal deoxynucleotidyl transferase. dATP: deoxyadenosine triphosphate. dTTP: deoxythymidine triphosphate. X-DNA-pAn and X-DNA-pTn: X-DNA motifs with poly-A and poly-T tails. ALP: alkaline phosphatase. ALP@DNAhg: ALP-encapsulated DNA hydrogels. Au@rGO: gold nanoparticles-functionalized reduced graphene oxide. GCE: glass carbon electrode. HP1, 2: hairpin DNA 1, 2. MCH: 6-mercaptohexanol. tDNA: target DNA. CV: cyclic voltammetry). |
| doi_str_mv | 10.1007/s00604-019-3283-2 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_proqu</sourceid><recordid>TN_cdi_proquest_miscellaneous_2179516689</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A715058283</galeid><sourcerecordid>A715058283</sourcerecordid><originalsourceid>FETCH-LOGICAL-c411t-49b2f67a0e78dcc5a419abac174918968d2943d9d28c08b7e120aef9a7eb75433</originalsourceid><addsrcrecordid>eNp9Ustu1DAUjRCIDoUPYIMssWGTYjsPJ8tRy0uqYANr68a-mXHr2MF2VKV_yR_hmWmRkBDywtY959yH7ymK14xeMErF-0hpS-uSsr6seFeV_EmxYXXVlg0V1dNiQylvy6oV_Kx4EeMNpUy0vH5enFVUsKZl1ab4dYUJw2QcJOMd8SPZ2vs9mgkDGYyfINzm19XXLRlWskTjdgQcQYsqBa-RTF6b0aAmdybtiXFlNGkhc0BlZpMgZWTydh20n_c-zvscIQoS2PU-Qzkn2FuwxiF5xCFiiU7BHBd71B-K71cd_A5tLq5JgrDDRHKNVdljR9NscxfqOMPL4tkINuKrh_u8-PHxw_fLz-X1t09fLrfXpaoZS2XdD3xsBVAUnVaqgZr1MIBiou5Z17ed5n1d6V7zTtFuEMg4BRx7EDiIpq6q8-LdKe8c_M8FY5KTiQqtBYd-iZIz0Tesbbs-U9-eqDuwKI0bfQqgDnS5zYugTZe3l1kX_2Dlo3EyyjscTY7_JWAngQo-xoCjnIPJG1slo_JgEHkyiMwGkQeDSJ41bx66XoYJ9R_FoyMygZ8IMUNuh0He-CW4_JP_yfobgnjKhQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2179516689</pqid></control><display><type>article</type><title>Determination of Alzheimer biomarker DNA by using an electrode modified with in-situ precipitated molybdophosphate catalyzed by alkaline phosphatase-encapsulated DNA hydrogel and target recycling amplification</title><source>MEDLINE</source><source>SpringerNature Journals</source><creator>Hua, Xiaoyu ; Zhou, Xingxing ; Guo, Shijing ; Zheng, Ting ; Yuan, Ruo ; Xu, Wenju</creator><creatorcontrib>Hua, Xiaoyu ; Zhou, Xingxing ; Guo, Shijing ; Zheng, Ting ; Yuan, Ruo ; Xu, Wenju</creatorcontrib><description>An electrochemical biosensor is described for highly sensitive determination of tDNA, an Alzheimer’s disease (AD)-related biomarker. Electroactive molybdophosphate anions were precipitated in-situ on a glassy carbon electrode (GCE) via catalytic hydrolysis by alkaline phosphatase (ALP). This is followed by recycling amplification of tDNA. Four DNA strands (referred to as S1, S2, S3 and S4) were designed to assemble X-shape DNA (X-DNA) building blocks. These were further extended into four directions under the action of DNA polymerase. The resultant two X-DNA motifs were polymerize. Simultaneously, ALP is encapsulated into a hydrogels network to obtain a porous material of type ALP@DNAhg. The GCE was modified with reduced graphene oxide functionalized with gold nanoparticles (Au@rGO). If ALP@DNAhg are captured via strand displacement, tDNA recycling assembly for signal amplification is initiated. This results in the immobilization of large amounts of ALP. On introduction of pyrophosphate and molybdate (MoO
4
2−
), ALP will catalyze the hydrolysis of pyrophosphate to produce phosphate. It will react with molybdate to form redox active phosphomolybdate anions (PMo
12
O
40
3−
). Its amperometrical signal depends on the concentration of tDNA in the 1.0 × 10
−2
to 1.0 × 10
4
pM concentration range, and the detection limit is 3.4 × 10
−3
pM.
Graphical abstract
Schematic presentation of (
a
) preparation of alkaline phosphatase-encapsulated DNA hydrogel (ALP@DNAhg). (
b
) fabrication of the biosensor for target DNA (tDNA) based on ALP@DNAhg to catalyze in situ precipitation of electroactive molybdophosphate anion (PMo
12
O
40
3−
) and tDNA recycling amplification, achieving tDNA-dependent electrochemical signal readout (X-DNA: X-shape DNA building block. TdT: terminal deoxynucleotidyl transferase. dATP: deoxyadenosine triphosphate. dTTP: deoxythymidine triphosphate. X-DNA-pAn and X-DNA-pTn: X-DNA motifs with poly-A and poly-T tails. ALP: alkaline phosphatase. ALP@DNAhg: ALP-encapsulated DNA hydrogels. Au@rGO: gold nanoparticles-functionalized reduced graphene oxide. GCE: glass carbon electrode. HP1, 2: hairpin DNA 1, 2. MCH: 6-mercaptohexanol. tDNA: target DNA. CV: cyclic voltammetry).</description><identifier>ISSN: 0026-3672</identifier><identifier>EISSN: 1436-5073</identifier><identifier>DOI: 10.1007/s00604-019-3283-2</identifier><identifier>PMID: 30715613</identifier><language>eng</language><publisher>Vienna: Springer Vienna</publisher><subject>Alkaline Phosphatase - metabolism ; Alzheimer Disease - diagnosis ; Alzheimer Disease - genetics ; Alzheimer's disease ; Analytical Chemistry ; Biomarkers ; Catalysis ; Characterization and Evaluation of Materials ; Chemistry ; Chemistry and Materials Science ; DNA ; DNA - analysis ; Electrochemical Techniques - methods ; Electrodes ; Humans ; Hydrogels ; Hydrolysis ; Microengineering ; Molybdenum ; Nanochemistry ; Nanotechnology ; Original Paper ; Phosphatases ; Phosphoric Acids</subject><ispartof>Mikrochimica acta (1966), 2019-03, Vol.186 (3), p.158-158, Article 158</ispartof><rights>Springer-Verlag GmbH Austria, part of Springer Nature 2019</rights><rights>COPYRIGHT 2019 Springer</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c411t-49b2f67a0e78dcc5a419abac174918968d2943d9d28c08b7e120aef9a7eb75433</citedby><cites>FETCH-LOGICAL-c411t-49b2f67a0e78dcc5a419abac174918968d2943d9d28c08b7e120aef9a7eb75433</cites><orcidid>0000-0002-2188-4621</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00604-019-3283-2$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00604-019-3283-2$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30715613$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hua, Xiaoyu</creatorcontrib><creatorcontrib>Zhou, Xingxing</creatorcontrib><creatorcontrib>Guo, Shijing</creatorcontrib><creatorcontrib>Zheng, Ting</creatorcontrib><creatorcontrib>Yuan, Ruo</creatorcontrib><creatorcontrib>Xu, Wenju</creatorcontrib><title>Determination of Alzheimer biomarker DNA by using an electrode modified with in-situ precipitated molybdophosphate catalyzed by alkaline phosphatase-encapsulated DNA hydrogel and target recycling amplification</title><title>Mikrochimica acta (1966)</title><addtitle>Microchim Acta</addtitle><addtitle>Mikrochim Acta</addtitle><description>An electrochemical biosensor is described for highly sensitive determination of tDNA, an Alzheimer’s disease (AD)-related biomarker. Electroactive molybdophosphate anions were precipitated in-situ on a glassy carbon electrode (GCE) via catalytic hydrolysis by alkaline phosphatase (ALP). This is followed by recycling amplification of tDNA. Four DNA strands (referred to as S1, S2, S3 and S4) were designed to assemble X-shape DNA (X-DNA) building blocks. These were further extended into four directions under the action of DNA polymerase. The resultant two X-DNA motifs were polymerize. Simultaneously, ALP is encapsulated into a hydrogels network to obtain a porous material of type ALP@DNAhg. The GCE was modified with reduced graphene oxide functionalized with gold nanoparticles (Au@rGO). If ALP@DNAhg are captured via strand displacement, tDNA recycling assembly for signal amplification is initiated. This results in the immobilization of large amounts of ALP. On introduction of pyrophosphate and molybdate (MoO
4
2−
), ALP will catalyze the hydrolysis of pyrophosphate to produce phosphate. It will react with molybdate to form redox active phosphomolybdate anions (PMo
12
O
40
3−
). Its amperometrical signal depends on the concentration of tDNA in the 1.0 × 10
−2
to 1.0 × 10
4
pM concentration range, and the detection limit is 3.4 × 10
−3
pM.
Graphical abstract
Schematic presentation of (
a
) preparation of alkaline phosphatase-encapsulated DNA hydrogel (ALP@DNAhg). (
b
) fabrication of the biosensor for target DNA (tDNA) based on ALP@DNAhg to catalyze in situ precipitation of electroactive molybdophosphate anion (PMo
12
O
40
3−
) and tDNA recycling amplification, achieving tDNA-dependent electrochemical signal readout (X-DNA: X-shape DNA building block. TdT: terminal deoxynucleotidyl transferase. dATP: deoxyadenosine triphosphate. dTTP: deoxythymidine triphosphate. X-DNA-pAn and X-DNA-pTn: X-DNA motifs with poly-A and poly-T tails. ALP: alkaline phosphatase. ALP@DNAhg: ALP-encapsulated DNA hydrogels. Au@rGO: gold nanoparticles-functionalized reduced graphene oxide. GCE: glass carbon electrode. HP1, 2: hairpin DNA 1, 2. MCH: 6-mercaptohexanol. tDNA: target DNA. CV: cyclic voltammetry).</description><subject>Alkaline Phosphatase - metabolism</subject><subject>Alzheimer Disease - diagnosis</subject><subject>Alzheimer Disease - genetics</subject><subject>Alzheimer's disease</subject><subject>Analytical Chemistry</subject><subject>Biomarkers</subject><subject>Catalysis</subject><subject>Characterization and Evaluation of Materials</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>DNA</subject><subject>DNA - analysis</subject><subject>Electrochemical Techniques - methods</subject><subject>Electrodes</subject><subject>Humans</subject><subject>Hydrogels</subject><subject>Hydrolysis</subject><subject>Microengineering</subject><subject>Molybdenum</subject><subject>Nanochemistry</subject><subject>Nanotechnology</subject><subject>Original Paper</subject><subject>Phosphatases</subject><subject>Phosphoric Acids</subject><issn>0026-3672</issn><issn>1436-5073</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9Ustu1DAUjRCIDoUPYIMssWGTYjsPJ8tRy0uqYANr68a-mXHr2MF2VKV_yR_hmWmRkBDywtY959yH7ymK14xeMErF-0hpS-uSsr6seFeV_EmxYXXVlg0V1dNiQylvy6oV_Kx4EeMNpUy0vH5enFVUsKZl1ab4dYUJw2QcJOMd8SPZ2vs9mgkDGYyfINzm19XXLRlWskTjdgQcQYsqBa-RTF6b0aAmdybtiXFlNGkhc0BlZpMgZWTydh20n_c-zvscIQoS2PU-Qzkn2FuwxiF5xCFiiU7BHBd71B-K71cd_A5tLq5JgrDDRHKNVdljR9NscxfqOMPL4tkINuKrh_u8-PHxw_fLz-X1t09fLrfXpaoZS2XdD3xsBVAUnVaqgZr1MIBiou5Z17ed5n1d6V7zTtFuEMg4BRx7EDiIpq6q8-LdKe8c_M8FY5KTiQqtBYd-iZIz0Tesbbs-U9-eqDuwKI0bfQqgDnS5zYugTZe3l1kX_2Dlo3EyyjscTY7_JWAngQo-xoCjnIPJG1slo_JgEHkyiMwGkQeDSJ41bx66XoYJ9R_FoyMygZ8IMUNuh0He-CW4_JP_yfobgnjKhQ</recordid><startdate>20190301</startdate><enddate>20190301</enddate><creator>Hua, Xiaoyu</creator><creator>Zhou, Xingxing</creator><creator>Guo, Shijing</creator><creator>Zheng, Ting</creator><creator>Yuan, Ruo</creator><creator>Xu, Wenju</creator><general>Springer Vienna</general><general>Springer</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-2188-4621</orcidid></search><sort><creationdate>20190301</creationdate><title>Determination of Alzheimer biomarker DNA by using an electrode modified with in-situ precipitated molybdophosphate catalyzed by alkaline phosphatase-encapsulated DNA hydrogel and target recycling amplification</title><author>Hua, Xiaoyu ; Zhou, Xingxing ; Guo, Shijing ; Zheng, Ting ; Yuan, Ruo ; Xu, Wenju</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c411t-49b2f67a0e78dcc5a419abac174918968d2943d9d28c08b7e120aef9a7eb75433</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Alkaline Phosphatase - metabolism</topic><topic>Alzheimer Disease - diagnosis</topic><topic>Alzheimer Disease - genetics</topic><topic>Alzheimer's disease</topic><topic>Analytical Chemistry</topic><topic>Biomarkers</topic><topic>Catalysis</topic><topic>Characterization and Evaluation of Materials</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>DNA</topic><topic>DNA - analysis</topic><topic>Electrochemical Techniques - methods</topic><topic>Electrodes</topic><topic>Humans</topic><topic>Hydrogels</topic><topic>Hydrolysis</topic><topic>Microengineering</topic><topic>Molybdenum</topic><topic>Nanochemistry</topic><topic>Nanotechnology</topic><topic>Original Paper</topic><topic>Phosphatases</topic><topic>Phosphoric Acids</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hua, Xiaoyu</creatorcontrib><creatorcontrib>Zhou, Xingxing</creatorcontrib><creatorcontrib>Guo, Shijing</creatorcontrib><creatorcontrib>Zheng, Ting</creatorcontrib><creatorcontrib>Yuan, Ruo</creatorcontrib><creatorcontrib>Xu, Wenju</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Mikrochimica acta (1966)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hua, Xiaoyu</au><au>Zhou, Xingxing</au><au>Guo, Shijing</au><au>Zheng, Ting</au><au>Yuan, Ruo</au><au>Xu, Wenju</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Determination of Alzheimer biomarker DNA by using an electrode modified with in-situ precipitated molybdophosphate catalyzed by alkaline phosphatase-encapsulated DNA hydrogel and target recycling amplification</atitle><jtitle>Mikrochimica acta (1966)</jtitle><stitle>Microchim Acta</stitle><addtitle>Mikrochim Acta</addtitle><date>2019-03-01</date><risdate>2019</risdate><volume>186</volume><issue>3</issue><spage>158</spage><epage>158</epage><pages>158-158</pages><artnum>158</artnum><issn>0026-3672</issn><eissn>1436-5073</eissn><abstract>An electrochemical biosensor is described for highly sensitive determination of tDNA, an Alzheimer’s disease (AD)-related biomarker. Electroactive molybdophosphate anions were precipitated in-situ on a glassy carbon electrode (GCE) via catalytic hydrolysis by alkaline phosphatase (ALP). This is followed by recycling amplification of tDNA. Four DNA strands (referred to as S1, S2, S3 and S4) were designed to assemble X-shape DNA (X-DNA) building blocks. These were further extended into four directions under the action of DNA polymerase. The resultant two X-DNA motifs were polymerize. Simultaneously, ALP is encapsulated into a hydrogels network to obtain a porous material of type ALP@DNAhg. The GCE was modified with reduced graphene oxide functionalized with gold nanoparticles (Au@rGO). If ALP@DNAhg are captured via strand displacement, tDNA recycling assembly for signal amplification is initiated. This results in the immobilization of large amounts of ALP. On introduction of pyrophosphate and molybdate (MoO
4
2−
), ALP will catalyze the hydrolysis of pyrophosphate to produce phosphate. It will react with molybdate to form redox active phosphomolybdate anions (PMo
12
O
40
3−
). Its amperometrical signal depends on the concentration of tDNA in the 1.0 × 10
−2
to 1.0 × 10
4
pM concentration range, and the detection limit is 3.4 × 10
−3
pM.
Graphical abstract
Schematic presentation of (
a
) preparation of alkaline phosphatase-encapsulated DNA hydrogel (ALP@DNAhg). (
b
) fabrication of the biosensor for target DNA (tDNA) based on ALP@DNAhg to catalyze in situ precipitation of electroactive molybdophosphate anion (PMo
12
O
40
3−
) and tDNA recycling amplification, achieving tDNA-dependent electrochemical signal readout (X-DNA: X-shape DNA building block. TdT: terminal deoxynucleotidyl transferase. dATP: deoxyadenosine triphosphate. dTTP: deoxythymidine triphosphate. X-DNA-pAn and X-DNA-pTn: X-DNA motifs with poly-A and poly-T tails. ALP: alkaline phosphatase. ALP@DNAhg: ALP-encapsulated DNA hydrogels. Au@rGO: gold nanoparticles-functionalized reduced graphene oxide. GCE: glass carbon electrode. HP1, 2: hairpin DNA 1, 2. MCH: 6-mercaptohexanol. tDNA: target DNA. CV: cyclic voltammetry).</abstract><cop>Vienna</cop><pub>Springer Vienna</pub><pmid>30715613</pmid><doi>10.1007/s00604-019-3283-2</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0002-2188-4621</orcidid></addata></record> |
| fulltext | fulltext |
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| ispartof | Mikrochimica acta (1966), 2019-03, Vol.186 (3), p.158-158, Article 158 |
| issn | 0026-3672 1436-5073 |
| language | eng |
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| source | MEDLINE; SpringerNature Journals |
| subjects | Alkaline Phosphatase - metabolism Alzheimer Disease - diagnosis Alzheimer Disease - genetics Alzheimer's disease Analytical Chemistry Biomarkers Catalysis Characterization and Evaluation of Materials Chemistry Chemistry and Materials Science DNA DNA - analysis Electrochemical Techniques - methods Electrodes Humans Hydrogels Hydrolysis Microengineering Molybdenum Nanochemistry Nanotechnology Original Paper Phosphatases Phosphoric Acids |
| title | Determination of Alzheimer biomarker DNA by using an electrode modified with in-situ precipitated molybdophosphate catalyzed by alkaline phosphatase-encapsulated DNA hydrogel and target recycling amplification |
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